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Djohan, Herlinda
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UJI EFEKTIVITAS KOMBINASI PERASAN BUAH LABU AIR DAN JAHE MERAH SEBAGAI ANTIBAKTERI Salmonella typhi Djohan, Herlinda; Triana, Linda; Nurhayati, Etiek
Jurnal Laboratorium Khatulistiwa Vol 8, No 1 (2024): November 2024
Publisher : poltekkes kemenkes pontianak

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.30602/jlk.v8i1.1584

Abstract

Typhoid fever is an acute disease caused by the bacteria Salmonella typhi. This bacteria is usually found in contaminated water or food. Apart from that, this bacteria can also be transmitted from infected people. The treatment carried out is using modern drugs such as the antibiotic chloramphenicol. However, the use of modern medicine has several disadvantages such as side effects. On the other hand, the use of natural ingredients as natural medicine has been widely used. One of them is that water gourd is empirically used as a fever reducer. Water gourd contains secondary metabolites, namely saponins, steroids and phenols which have been proven to have antibacterial activity and are able to inhibit the growth of Salmonella typhi bacteria. Another natural ingredient that has antibacterial activity is red ginger. Red ginger contains active compounds that have antibacterial effects such as flavonoids, phenols, treponoids and essential oils. Antibacterial activity was obtained by measuring the diameter of the inhibition zone after administering 4 variations of the concentration of the juice sample. The positive control used amoxicillin 30 µg/disk and the negative control used distilled water. The research design used was a quasi-experimental method. The samples were water gourd and red ginger juice with varying concentrations of 40%, 60%, 80% and 100% and the average inhibition zone values were obtained, namely 8.875 mm, 9.875 mm, 11.25 mm and 14.25 mm. Based on the results of research and computerized data processing using the Kruskall Walis test, a value of p = 0.000 (p<0.05) was obtained, which means that there is an effect of the concentration of pumpkin juice and red ginger on the inhibitory power of Salmonella thypi bacteria.
KAJIAN TENTANG PENURUNAN JUMLAH DONOR DARAH AKIBAT PANDEMI COVID-19: DATA SELAMA 4 TAHUN DARI UDD PMI KOTA PONTIANAK Aditia, Aditia; Samwidya, Veronika Sriayu; Nuswantoro, Ari; Djohan, Herlinda; Supriyanto, Supriyanto; Reza, Nur
Media Penelitian dan Pengembangan Kesehatan Vol. 32 No. 4 (2022): MEDIA PENELITIAN DAN PENGEMBANGAN KESEHATAN
Publisher : Poltekkes Kemenkes Bandung

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.34011/jmp2k.v32i4.1890

Abstract

The COVID-19 pandemic has left a noticeable mark on the health care sector. One such example is the blood services provided by the Blood Donor Unit (UDD) of Indonesian Red Cross (PMI). Every time there was an increase in COVID-19 cases, blood donation activities sharply decreased, according to the UDD PMI. During the COVID-19 pandemic, there was a scarcity of blood donors. This research aimed to see the difference in the number of blood donors before and during the pandemic. This research was descriptive analytical with a cross-sectional design by collecting data on the number of blood donors at UDD PMI Pontianak City from 2018 to 2022. The results of the study show that the number of blood donors before the pandemic was 52,607 donors, while the number of blood donors during the pandemic was 48,421 donors. which means there was a decrease in the number of blood donors by 4,186 donors (7.96%). The Independent Samples Test obtained a significant value of 0.045 (p < 0.05), which means there was a difference in the number of blood donors before and during the COVID-19 pandemic at UDD PMI Pontianak City. Concerns about the risk of infection and government policies limiting activities outside the home can explain why the COVID-19 pandemic has resulted in a decline in blood donation rates.
HUBUNGAN MENGKONSUMSI TABLET BESI TERHADAP KADAR HEMOGLOBIN PADA IBU HAMIL TRIMESTER DUA DI PUSKESMAS SIANTAN TENGAH KELURAHAN SIANTAN TENGAH Sutriswanto, Sutriswanto; Slamet, Slamet; Djohan, Herlinda; Febrianti, Aprilia
Jurnal Laboratorium Khatulistiwa Vol 8, No 2 (2025): Mei 2025
Publisher : poltekkes kemenkes pontianak

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.30602/jlk.v8i2.1854

Abstract

Anemia adalah penurunan kapasitas darah dalam membawa oksigen karena jumlah sel darah merah yang kurang dari normal bisa disebabkan kehilangan banyak darah (Parulian et al., 2016). Anemia kehamilan disebut potensi membahayakan ibu dan anak, karena itulah anemia memerlukan perhatian serius dari semua pihak yang terkait dalam pelayanan kesehatan (Pratiwi & Fatimah, 2019). Beberapa penelitian melaporkan bahwa pemberian tablet Fe dapat meningkatkan kadar hemoglobin serta mengurangi kejadian anemia pada ibu hamil. Penelitian ini bertujuan untuk mengetahui apakah terdapat perbedaan kadar hemoglobin ibu hamil trimester dua sebelum dan sesudah mengkonsumsi tablet Fe di Puskesmas Siantan Tengah Kelurahan Siantan Tengah. Metode yang digunakan dalam peneltian ini adalah metode Automatic Hematology Analyzer. Sampel yang diencerkann dengan larutan elektrolit dialirkan melalui micro-apertura yang telah terkalibrasi. Hasil penelitian hasil uji normalitas bahwa nilai p-value bernilai 0,108 dan 0,200 dimana lebih besar bila dibandingkan taraf signifikansi 0,05, sehingga data berdistribusi normal. Hasil pengujian homogenitas nilai signifikansi bernilai 0,983 dimana lebih besar dibandingkan 0,05, data tersebut memiliki varians yang sama (homogen). Kesimpulan dari penelitian yang telah dilakukan terdapat perbedaan kadar hemoglobin pada ibu hamil trimester dua sebelum dan sesudah mengkonsumsi tablet Fe di Puskesmas Siantan Tengah Kelurahan Siantan Tengah dengan hasil uji diketahui nilai sig. (2-tailed) sebesar 0,000 < 0,0, maka Ho diterima. Bahwa ada perbedaan rata-rata antara hasil pemeriksaan pertama dan persamaan kedua.
Gambaran Kadar Glukosa Darah Puasa Pada Lanjut Usia (Lansia) di Puskesmas Kota Pontianak Agustriana, Fadhila; Nurhayati, Etiek; Salim, Maulidiyah; Djohan, Herlinda
Jurnal Laboratorium Khatulistiwa Vol 8, No 2 (2025): Mei 2025
Publisher : poltekkes kemenkes pontianak

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.30602/jlk.v8i2.1890

Abstract

Lanjut usia (lansia) merupakan kelompok usia ≥60 tahun yang rentan mengalami berbagai masalah kesehatan, salah satunya adalah gangguan metabolisme glukosa yang dapat berujung pada diabetes melitus. Kadar glukosa darah yang tidak terkontrol dapat meningkatkan risiko komplikasi kronis dan memperburuk kualitas hidup lansia. Penelitian ini bertujuan untuk menggambarkan kadar glukosa darah puasa (GDP) pada lansia yang berkunjung ke Puskesmas Perumnas II Sungai Beliung, Kota Pontianak periode Januari-Desember 2024. Penelitian ini menggunakan desain deskriptif dengan pendekatan cross-sectional. Sampel berjumlah 561 orang lansia yang dipilih menggunakan teknik purposive sampling dengan kriteria inklusi lansia yang melakukan pemeriksaan GDP di Puskesmas Perumnas II, sedangkan kriteria ekslusinya adalah lansia yang melakukan pemeriksaan glukosa darah sewaktu di Puskesmas Perumnas II. Hasil penelitian menunjukkan bahwa dari 561 lansia yang melakukan pemeriksaan GDP terdapat 379 (67,56%) lansia yang memiliki kadar GDP ≥126 dan hanya ada 182 (32,44%) lansia yang memiliki kadar GDP <126. Kurangnya aktivitas fisik, usia lanjut dan pola makan tinggi karbohidrat adalah faktor-faktor yang berkontribusi terhadap peningkatan kadar glukosa darah. Penelitian ini merekomendasikan perlunya edukasi berkelanjutan dan pemantauan rutin kadar glukosa darah puasa pada lansia di tingkat pelayanan primer guna mencegah komplikasi yang lebih lanjut.
The relationship between blood albumin and the incidence of preeclampsia in pregnant women Shodiq, Ja'far; Nurhayati, Etiek; Djohan, Herlinda
ELECTRON (Journal of Science and Technology) Vol. 7 No. 2 (2025): ELECTRON (Journal of Science and Technology)
Publisher : Borneo Scientific Publishing

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.53770/electron.v7i2.517

Abstract

Maternal Mortality Rate (MMR) is one of the important indicators of public health status. Maternal Mortality Rate (MMR) in West Kalimantan Province was 131 cases. The cause of this maternal mortality rate (22.61%) is hypertension in pregnancy. If pregnant women experience a lack of albumin levels (Hypoalbuminemia) then fetal development will be hampered, it can even cause babies to be born malnourished. Pregnant women who have low albumin levels will have a higher risk of preeclampsia. The purpose of this study was to determine the relationship between blood albumin and the incidence of preeclampsia in pregnant women and examine blood albumin as a risk factor for the incidence of preeclampsia in pregnant women. This research method is descriptive analytic with a cross-sectional approach, The population of this study were pregnant women who performed pregnancy checks at the Tanjungpura University Pratama Clinic. The sampling technique used was Incidental Sampling with a sample size of 43 respondents. The results of the analysis using the Chi-Square test obtained a p-value on Fisher's Exact Test of 0.001 with a sig limit (<0.05). And obtained the odd risk value in the risk estimate table of 20.3. The conclusion of this study shows that there is an association between blood albumin and the incidence of preeclampsia. And blood albumin can be used as a risk factor for the occurrence of preeclampsia in pregnant women.
Exploring C-Reactive Protein Levels in Menstruating Female Students: A Descriptive Study Khoirunzia, Agiesa; Djohan, Herlinda; Triana, Linda; Nurhayati, Etiek
MEDICA (International Medical Scientific Journal) Vol. 7 No. 1 (2025): MEDICA (Internasional Medical Scientific Journal)
Publisher : Borneo Scientific Publishing

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.53770/medica.v7i1.482

Abstract

During menstruation, prostaglandins induce uterine contractions, which contribute to the thickening of the endometrial mucosa and the constriction of blood vessels. Elevated levels of prostaglandins may influence C-Reactive Protein (CRP) levels, a marker that increases in response to inflammation or infection as part of the body's immune response. This study aimed to examine CRP levels in female students during menstruation. A descriptive research design was employed to assess CRP levels in menstruating female students. The sample consisted of blood serum collected from 30 students at Madrasah Aliyah Pondok Pesantren Pembangunan Ushuluddin in Singkawang City. The results showed that among the participants, 5 students aged 16 and 1 student aged 15 exhibited positive CRP levels. Regarding the duration of menstruation, positive CRP levels were detected in 4 students on the first day, 1 student on the second day, and 1 student on the third day. Overall, 20% of the students showed positive CRP levels, while 80% had negative CRP levels. The conclusion indicates that a subset of menstruating female students exhibited elevated CRP levels, particularly during the early days of menstruation. This suggests that menstruation, likely through prostaglandin-induced inflammation, may be associated with an increase in CRP levels in some individuals.
The Relationship Between NS1 Examination and the Examination of Hemoglobin, Hematocrit, Leukocytes, Platelets, and Erythrocytes in Dengue Fever Patients in The Pediatric Ward Ratulangi, Agus Dimas; Djohan, Herlinda; Kamilla, Laila
MEDICA (International Medical Scientific Journal) Vol. 7 No. 1 (2025): MEDICA (Internasional Medical Scientific Journal)
Publisher : Borneo Scientific Publishing

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.53770/medica.v7i1.486

Abstract

The NS1 antigen test is developed to detect dengue virus infection during the acute phase, a stage marked by various hematological abnormalities. An increase in hemoglobin levels greater than 20% can support the diagnosis of Dengue Hemorrhagic Fever (DHF), often caused by hemoconcentration due to plasma leakage, which also raises erythrocyte levels in the bloodstream. This study aimed to examine the relationship between NS1 test results and hemoglobin, hematocrit, leukocyte, platelet, and erythrocyte levels in pediatric DHF patients at Dr. Soedarso Regional Hospital. Using a cross-sectional design with total sampling, 203 samples were collected from suspected pediatric DHF patients between May and July 2023. Data included NS1 results and hematology parameters. The analysis showed a significant association between positive NS1 results and hemoglobin levels (p = 0.025) as well as platelet counts (p = 0.035), but no significant relationship with hematocrit (p = 0.872), leukocytes (p = 0.269), or erythrocytes (p = 0.060). In conclusion, NS1 positivity is significantly associated with hemoglobin levels and platelet count, but not with hematocrit, leukocytes, or erythrocytes in pediatric DHF patients.
Phytochemical Identification and Antioxidant Activity Test of Acetone Extract of Celery Leaves (Apium graveolens L.) Using the DPPH (2,2-diphenyl-1-picrylhydrazyl) Method Auliannisa, Riska; Jenny, Gervacia; Djohan, Herlinda
MEDICA (International Medical Scientific Journal) Vol. 6 No. 3 (2024): MEDICA (Internasional Medical Scientific Journal)
Publisher : Borneo Scientific Publishing

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.53770/medica.v6i3.488

Abstract

Free radicals are formed in the body through oxidation processes and cell combustion during breathing, metabolism, excessive physical activity, as well as exposure to external pollution. Antioxidants play an important role in protecting the body from oxidative damage. Celery leaves (Apium graveolens L.), which are rich in flavonoid compounds, have the potential to be a natural source of antioxidants. This study aims to identify the secondary metabolite content and measure the antioxidant activity of acetone extract of celery leaves based on IC50 values using the DPPH (2,2-diphenyl-1-picrylhydrazyl) method. The study uses a quasi-experimental design with purposive sampling techniques. The antioxidant activity of acetone extract of celery leaves at concentrations of 30 ppm, 50 ppm, 70 ppm, and 90 ppm was tested using a UV-VIS spectrophotometer at a wavelength of 516 nm. The results showed the presence of secondary metabolite compounds such as alkaloids, flavonoids, phenols, triterpenoids, saponins, and tannins in the acetone extract of celery leaves. The IC50 value of the acetone extract of celery leaves was 115.09490 ppm, indicating moderate antioxidant activity (IC50 value between 100–250 ppm). These findings conclude that acetone extract of celery leaves has potential to be used as a natural antioxidant source.
POTENSI JUS BAYAM MERAH (AMARANTHUS TRICOLOR L) SEBAGAI ALTERNATIF PEWARNAAN TELUR CACING SOIL TRANSMITTED HELMINTH Artanti, Lina Yunda; Sungkawa, Hendra Budi; Djohan, Herlinda; Alfianita, Riska
PREPOTIF : JURNAL KESEHATAN MASYARAKAT Vol. 8 No. 2 (2024): AGUSTUS 2024
Publisher : Universitas Pahlawan

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.31004/prepotif.v8i2.30402

Abstract

Cacing yang ditularkan dari tanah adalah cacing yang menginfeksi manusia melalui tanah. Identifikasi telur cacing dilakukan secara mikroskopis menggunakan teknik pewarnaan. Pewarna yang digunakan dalam memeriksa telur cacing menggunakan 2% eosin. Namun, eosin memiliki kekurangan karena tidak mudah terurai, sehingga diperlukan pewarna alternatif yang lebih ramah lingkungan. Tanaman bayam merah berpotensi untuk digunakan sebagai pewarna alami karena mengandung senyawa antosianin. Penelitian ini bertujuan untuk mengetahui potensi sari bayam merah (amaranthus tricolor L) sebagai alternatif pewarna telur cacing yang ditularkan melalui tanah. Desain penelitian eksperimen semu (quasi experiment) dengan teknik purposive sampling. Sampel yang digunakan adalah sari batang amaranthus tricolor L (amaranthus tricolor L) dengan perbandingan 1:1, 1:2, 1:3, 1:4, dan 1:5 menggunakan metode langsung dan diulang sebanyak 5 kali. Berdasarkan hasil penelitian, rasio 1:1 jus batang bayam merah menunjukkan hasil 60%, rasio 1:2 menunjukkan hasil 53%, rasio 1:3 menunjukkan hasil 53%, rasio 1:4 menunjukkan hasil 40% dan rasio 1:5 menunjukkan hasil 40%. Kesimpulan dari penelitian ini adalah konsentrasi 1:1 memiliki kualitas tertinggi yang dianggap berpotensi sebagai alternatif pewarnaan.
Potensi Air Perasan Batang Bayam Merah (Amaranthus Tricolor L) Sebagai Alternatif Pewarnaan Telur Cacing Soil Transmitted Helminth Artanti, Lina Yunda; Sungkawa, Hendra Budi; Djohan, Herlinda; Nuswantoro, Ari; Alfianita, Riska
Innovative: Journal Of Social Science Research Vol. 4 No. 4 (2024): Innovative: Journal Of Social Science Research
Publisher : Universitas Pahlawan Tuanku Tambusai

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.31004/innovative.v4i4.13043

Abstract

in the examination of worm eggs uses 2% eosin. However, eosin has shortcomings because it is not easily decomposed, so an alternative coloring that is more environmentally friendly is needed. Red spinach plants have the potential to be used as a natural dye because they contain anthocyanin compounds. This study aims to determine the potential of red spinach stem juice (amaranthus tricolor L) as an alternative to staining soil transmitted helminth eggs. Research design of quasi experiment with purposive sampling technique. Samples used red spinach stem juice (amaranthus tricolor L) in a ratio of 1:1, 1:2, 1:3, 1:4, and 1:5 by direct method and repeated 5 times. Based on the results of the research, the juice of red spinach stems in the ratio of 1:1 showed 60% results, the ratio of 1:2 showed 53% results, the ratio of 1:3 showed 53% results, the ratio of 1:4 showed 40% results and the ratio of 1:5 showed 40% results. The conclusion of this study is that the concentration of 1: 1 has the highest quality which is considered to have potential as an alternative coloring.
Co-Authors Aditia, Aditia Affifatuzahara, Affifatuzahara Agum, Dexa Agustriana, Fadhila Al-qorifah, Fahma Ajeng Alfianita, Riska Anjelica, Benedikta Ari Nuswantoro Artanti, Lina Yunda Auliannisa, Riska Budi Sungkawa, Hendra Chitra, Fara Christian , Diky Febrianti, Aprilia Furwanti, Endang Sri Hendra Budi Sungkawa, Hendra Budi Ihsan, Bagus Muhammad Ika Sartika Imma Fatayati Khoirunzia, Agiesa Laila Kamilla Linda Triana Maulidiyah Salim Muhammad Ihsan, Bagus Ningsih, Nur Reza nirnadia, nirnadia Nurhayati, Etiek Nurhayati, Widya Pratama, Victorinus Alfino PUTRI, SILVIA Ratnawaty, Gervacia Jenny Ratulangi, Agus Dimas Regita, Lidia Reza, Nur Roofiif, Sayyid Al Samwidya, Veronika Sriayu Shafitri, Anisya Nur Shodiq, Ja'far Sidiq, Alfino Validita Slamet . Sugito - Supriyanto Supriyanto Sutriswanto, Sutriswanto Suwandi, Edy Trifani, Paula Jaenne Tumpuk, Sri Ulfa, Nur Uq'ma, Nurul Widiyanti, Leonita Wulandari, Rizky Astri