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INHIBITORY POTENTIAL OF BUTTERFLY PEA FLOWER (CLITORIA TERNATEA L) DECOCTION AGAINST ESCHERICHIA COLI BACTERIA Christian , Diky; Ihsan, Bagus Muhammad; Supriyanto, Supriyanto; Djohan, Herlinda
SINERGI : Jurnal Riset Ilmiah Vol. 2 No. 11 (2025): SINERGI : Jurnal Riset Ilmiah, November 2025
Publisher : Lembaga Pendidikan dan Penelitian Manggala Institute

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.62335/sinergi.v2i11.1947

Abstract

Penelitian ini bertujuan untuk mengetahui potensi daya hambat rebusan bunga telang (Clitoria ternatea L.) terhadap pertumbuhan Escherichia coli serta menilai efektivitas tiga konsentrasi larutan yang digunakan. Fokus penelitian diarahkan untuk menjawab apakah rebusan bunga telang dapat menghasilkan zona hambat ketika diuji menggunakan prosedur standar pada bakteri uji. Metode penelitian menggunakan rancangan eksperimental dengan uji difusi cakram Kirby–Bauer pada media Mueller Hinton Agar. Rebusan bunga telang disiapkan dalam konsentrasi 40%, 50%, dan 60%, kemudian diuji melalui enam kali replikasi pada setiap kelompok perlakuan. Pengamatan dilakukan setelah inkubasi untuk menentukan ada atau tidaknya zona hambat. Hasil penelitian menunjukkan bahwa seluruh konsentrasi rebusan tidak menghasilkan zona bening, sehingga tidak terdapat aktivitas antibakteri terhadap E. coli. Meskipun skrining fitokimia menunjukkan adanya flavonoid, saponin, dan tanin, senyawa tersebut tidak memberikan efek penghambatan melalui proses perebusan air. Temuan ini mengindikasikan bahwa metode ekstraksi air kurang efektif untuk menghasilkan komponen aktif yang mampu menghambat bakteri. Kesimpulannya, rebusan bunga telang pada konsentrasi 40–60% tidak memiliki daya hambat terhadap Escherichia coli. .
Gambaran Kadar Protein Total pada Ibu Hamil di Wilayah Kerja Puskesmas Purnama, Kota Pontianak Regita, Lidia; Nurhayati, Etiek; Tumpuk, Sri; Djohan, Herlinda
SENTRI: Jurnal Riset Ilmiah Vol. 4 No. 11 (2025): SENTRI : Jurnal Riset Ilmiah, November 2025
Publisher : LPPM Institut Pendidikan Nusantara Global

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.55681/sentri.v4i11.4964

Abstract

Maternal Mortality Rate is one of the indicators for assessing the quality of services place. In West Kalimantan Province, AKI is the highest serious concern, based on data that the local Health Office recorded about 432 cases of maternal deaths between 2022 and 2024. Preeclampsia, a severe pregnancy complication characterized by hypertension and proteinuria, has been identified as a major cause of this mortality. Early detection of proteinuria, which can be done through a simple urine test, is a key strategy for risk mitigation. This study aims to determine the prevalence of proteinuria levels among pregnant women in the working area of the Purnama Community Health Center, Pontianak City, as part of an effort for early detection. The research method used is a quantitative descriptive study with a case study approach, involving 30 pregnant women as samples. The examination was performed using the urine dipstick method. Demographic data, specifically age and blood type, as well as the proteinuria test results, were analyzed descriptively to identify their frequency and percentage. The study's results show that out of the 30 samples, 13 (43.3%) tested positive for proteinuria, with a breakdown of 7 samples showing trace positive (+ Protein), 4 samples showing positive (Pro 1 (+)), and 2 samples showing positive (Pro 2 (+)). These findings confirm a significant prevalence of preeclampsia risk among the sample population and underscore the importance of routine screening programs and further clinical evaluation for pregnant women who test positive.
Pre-Analytical Stability of Fasting Blood Glucose: A Comparison Between Immediate Analysis and Two-Hour Room Temperature Storage Sugito, Sugito; Djohan, Herlinda; Tumpuk, Sri; Sidiq, Alfino Validita; Agum, Dexa
Jurnal Laboratorium Khatulistiwa Vol 9, No 1 (2025): November 2025
Publisher : poltekkes kemenkes pontianak

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.30602/jlk.v9i1.2138

Abstract

The stability of blood samples for glucose testing is a critical preanalytical factor. In vitro glycolysis at room temperature can significantly decrease glucose levels, potentially leading to inaccurate diagnostic results. This study aimed to compare fasting blood glucose levels examined immediately and those stored for 2 h at room temperature. This descriptive, cross-sectional study involved 35 serum samples from participants undergoing fasting blood glucose tests at the Sintang Regional Health Laboratory. Each sample was split into two aliquots: one was analyzed immediately after processing (baseline), and the other was analyzed after being stored for 2 hours at room temperature (25-30°C). Glucose levels were measured using the GOD-PAP method with a Microlab 300 spectrophotometer. Statistical analysis was performed using the Shapiro-Wilk normality test and paired T-test to compare the differences in glucose levels between the two groups. The mean glucose level examined immediately was 90.8 mg/dL (SD=5.098), while the level after the 2-hour delay was 85.8 mg/dL (SD=4.451), showing an average decrease of 5.0 mg/dL. The data were normally distributed (p=0.129), and the paired T-test showed a highly statistically significant difference (p<0.001) between the two examination conditions. Storing serum samples at room temperature for 2 h significantly decreased glucose levels. This finding underscores the importance of immediate sample processing for glucose testing to ensure accurate diagnosis. If a delay is unavoidable, sample refrigeration is highly recommended to inhibit the glycolysis process.