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All Journal HAYATI Journal of Biosciences VALENSI Tadris: Jurnal keguruan dan Ilmu Tarbiyah Indonesian Journal of Biotechnology Science and Technology Indonesia Jurnal Penelitian Pendidikan IPA (JPPIPA) Jurnal SOLMA Jurnal Riset Pendidikan Kimia (JRPK) Jurnal Riset Sains dan Kimia Terapan JSMARTech : Journal of Smart Bioprospecting and Technology Makara Journal of Science SEGARA : Jurnal Pengabdian Kepada Masyarakat Jurnal Kimia Unand Jurnal Bioteknologi & Biosains Indonesia (JBBI) Jurnal Bioteknologi & Biosains Indonesia
Kurniadewi, Fera
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Agriculture, Biological Sciences & Forestry Humanities Biochemistry, Genetics & Molecular Biology Chemical Engineering, Chemistry & Bioengineering Chemistry Dentistry Earth & Planetary Sciences Education Energy Environmental Science Health Professions Immunology & microbiology Materials Science & Nanotechnology Medicine & Pharmacology Neuroscience Physics Other

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DETERMINATION OF OPTIMAL ANNEALING TEMPERATURE Vibrio alginolyticus PRIMERS USING POLYMERASE CHAIN REACTION METHOD Putri, Gladys Indira; Nurjayadi, Muktiningsih; Declan, Jefferson Lynford; Krisdawati, Ismaya; Juliansyah, Dandy Akbar; Fahriza, Tiara; Azzahra, Maharanianska; Maulana, Irvan; Kartika, Irma Ratna; Kurniadewi, Fera; Sukmawati, Dalia; Saamia4, Vira; Saputro, Dwi Anna Oktaviani; Wiranatha, I Made; Abomoelak, Bassam; El-Enshasy, Hesham Ali
Jurnal Bioteknologi & Biosains Indonesia (JBBI) Vol. 10 No. 2 (2023)
Publisher : BRIN - Badan Riset dan Inovasi Nasional

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.55981/jbbi.2023.2976

Abstract

Food poisoning is a global issue of grave concern. If food is not properly cooked, it can be a medium for the spread of pathogenic bacteria. Vibrio alginolyticus is one of the pathogenic bacteria that can cause food poisoning. real-time Polymerase Chain Reaction (rt-PCR) can detect pathogenic bacteria in food, so it is necessary to determine the optimal annealing temperature. This research aims to obtain the optimal annealing temperature of the Va_Chr1_FR primer using Gradient PCR. The DNA concentration used was 174.5 with an A260/A280 purity of 1.94. The temperature range tested, 53°C-62°C, corresponds to the melting temperature of the Va_Chr1_FR primers. The primers designed were F5'-TTCTTCTGTTGTAGGTTCCG-F3' and R5'-CCAGCCCTCACATCTAATAC-R3'. Based on these results, a temperature of 60°C is deemed as the most optimal annealing temperature because it produces one of the brightest bands on electrophoresis with an amplicon length of 146 bp. The findings of this study will be beneficial to the development of Va_Chr1_FR Vibrio alginolyticus primers testing on food samples using the real-time PCR method. 
Experimental and Molecular Docking Study of 3′,4′,5′-Trimethoxychalcones Targeting Overexpressed Protein in HCT-116 Colon Cancer Cells Danova, Ade; Wonganan, Piyanuch; Hermawati, Elvira; Kurniadewi, Fera; Musthapa, Iqbal; Chavasiri, Warinthorn
Jurnal Kimia Valensi Jurnal Kimia VALENSI, Volume 11, No. 2, November 2025
Publisher : Department of Chemistry, Faculty of Science and Technology Syarif Hidayatullah Jakarta State Islamic University

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.15408/jkv.v11i2.46483

Abstract

Cancer poses a substantial global health challenge. Colorectal cancer (CRC) is the second leading cause of cancer-related mortality after lung cancer and is associated with high mortality rates worldwide. Chalcones have attracted significant interest because of their diverse biological properties, including potential anticancer effects. In this study, five 3′,4′,5′-trimethoxychalcones (1-5) were tested against HCT-116 colon cancer cells using an MTT assay for the first time. Molecular docking was conducted to predict molecular interactions targeting three proteins (tubulin, EGFR, and CDK2). Among the five, four compounds (1, 3, 4, and 5) exhibited strong inhibitory activity against HCT-116 colon cells, with IC50 values < 10 µM. Compounds 1-5 showed potency as drug candidates based on the Lipinski rules and pharmacokinetic profiles using SwissADME and pkCSM online tools. Moreover, molecular docking was performed on compound 5 against three protein targets (tubulin, EGFR, CDK2) with binding affinities of -7.4, -7.3, and -8.5 kcal/mol, respectively, and showed major H-bond interactions. Therefore, these results suggest that compound 5 could be a potential inhibitor to be developed in future studies, both in vitro and in vivo, to understand its inhibition mechanism and efficacy.
Pelatihan Pemanfaatan Aplikasi Gimkit untuk Guru Kimia Madrasah Aliyah (MA) di MGMP Kimia Provinsi DKI Jakarta Kartika, Irma Ratna; Kurniadewi, Fera; Widiawan, Ages Kayla Shyfa; Nugroho, Aidahaya Hanannisa; Handayani, Fitria; Dewanti, Farradilla Alenia; Irwanto, Irwanto; Susilo, Ahmad Dwi; Barkah, Eka; Saputra, Irwan; Allanas, Edith; Laurensia, Laurensia; Asworo, Yuliana Dwi; Rustini, Yeyet; Kartikawati, Nindya Galuh
Jurnal SOLMA Vol. 14 No. 3 (2025)
Publisher : Universitas Muhammadiyah Prof. DR. Hamka (UHAMKA Press)

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.22236/solma.v14i3.20603

Abstract

Pendahuluan: Penurunan skor sains siswa Indonesia dari 403 poin (tahun 2015) menjadi 383 poin (tahun 2022) mendorong penerapan Kurikulum Merdeka berbasis digital dan inovatif. Sehingga diperlukan pelatihan pembuatan soal interaktif berbasis game online yang bertujuan membekali guru Kimia Madrasah Aliyah (MA) Provinsi DKI Jakarta yang tergabung dalam Musyawarah Guru Mata Pelajaran (MGMP) untuk menguasai pembuatan soal interaktif berbasis game online guna mempersiapkan siswa menghadapi ujian berbasis teknologi serta mengurangi kecemasan siswa. Metode: Kegiatan dilakukan secara luring dan daring pada 20 guru melalui tahap sosialisasi informasi, pelatihan, dan evaluasi. Hasil: Kegiatan pelatihan meningkatkan pemahaman dan penguasaan guru Kimia MA tentang pembuatan soal interaktif berbasis game online, yaitu Gimkit. Kesimpulan: Kegiatan ini berhasil meningkatkan kompetensi pedagogik guru Kimia MA dalam pemanfaatan teknologi, serta berkontribusi terhadap peningkatan kualitas pembelajaran di MGMP melalui penggunaan aplikasi Gimkit.
Co-Authors Aboemolak, Bassam Abomoelak, Bassam Abumoelak, Bassam Achmad Ridwan, Achmad Ade Danova Agus Setiawan Akbar, Dandy Allanas, Edith Amyyana, Afwu Hayyi Angelina, Helzi Anggraeni, Rosita Gio Anis, Mhd. Aqilah, Amadita Shafa Asworo, Yuliana Dwi Aulia Rachma, Lu'luunnisa Azizah, Puan Aqila Azzahra, Maharanianska Barkah, Eka Berkahingrum, Ayu Bunga Falana, Isfasona Chavasiri, Warinthorn Chen, Shyi-Tien Dalia Sukmawati Danova, Ade Darmiyanti, Waskitarini Darwis, Zulmanelis Dewanti, Farradilla Alenia Didin Mujahidin El Enshasy, Hesham El-Enshasy, Hesham Ali Elenshasy, Hesham Ali Enshasy, Hesham Ali El Fahriza, Tiara Fitria Handayani Fitriyanti, Anisa Ghufrooni, Rifqi Grace, Grace Hadi Nugrahaningsih, Tritiyatma Hadinugrahaningsih, Tritiyatma Hermawati, Elvira Iqbal Musthapa Irwanto Irwanto Istantia Chantika, Kinanti Jefferson Lynford Declan Juliansyah, Dandy Akbar Kartika, Irma Kartika, Irma Ratna Kartikawati, Nindya Galuh Krisdawati, Ismaya Laurensia, Laurensia Maria Paristiowati Maulana, Irvan Muktiningsih Nurjayadi Musie, Royna Rahma Nugroho, Aidahaya Hanannisa Nurhayadi, Muktiningsih Nurul Fadillah Octaviany, Sheila Putri Fajriani, Fathya Putri, Adinda Myra Amalia Putri, Gladys Indira Putri, Gusti Angieta Rahmawati, Atikah Nur Ratna Kartika, Irma Rosita Rosita Rustini, Yeyet Saamia, Vira Saamia4, Vira Saputra, Irwan Saputro, Dwi Anna Oktaviani Sari, Ely Puspita Septiani, Elsa Shangkara, Muhammad Arkent Sihombing, Ananda Indah Putri Siti Fatimah SRI RAHAYU Susilo, Ahmad Dwi Syafriani, Yolanda Febrica Umiatin, Umiatin Widiawan, Ages Kayla Shyfa Wiranatha, I Made Wonganan, Piyanuch Yuli Rahmawati