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Andini, Ary
Program Studi Analis Kesehatan, Fakultas Kesehatan, Universitas Nahdlatul Ulama Surabaya
Religion Agriculture, Biological Sciences & Forestry Arts Humanities Biochemistry, Genetics & Molecular Biology Chemical Engineering, Chemistry & Bioengineering Chemistry Civil Engineering, Building, Construction & Architecture Computer Science & IT Dentistry Economics, Econometrics & Finance Education Electrical & Electronics Engineering Engineering Environmental Science Health Professions Immunology & microbiology Languange, Linguistic, Communication & Media Law, Crime, Criminology & Criminal Justice Library & Information Science Mathematics Medicine & Pharmacology Neuroscience Nursing Public Health Social Sciences Veterinary Other

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Journal : INDONESIAN JOURNAL OF MEDICAL LABORATORY SCIENCE AND TECHNOLOGY

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CYTOTOXICITY ASSAY USING BRINE SHRIMP LETHALITY TEST ON COLLAGEN-CHITOSAN WOND DRESSING STERILIZED BY ULTRAVIOLET LIGHT Ary Andini; Endah Prayekti; Devyana Dyah Wulandari; Ersalina Nidianti
JURNAL INDONESIA DARI ILMU LABORATORIUM MEDIS DAN TEKNOLOGI Vol 2 No 1 (2020): Laboratory Examinations Support in Medical Toxicology
Publisher : Universitas Nahdlatul Ulama Surabaya

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.33086/ijmlst.v2i1.1467

Abstract

Collagen gives a moist state on the wound area to accelerate the wound healing process. Chitosan is a polymer as known as non-toxic, antibacterial, antifungal, biodegradable, and biocompatible materials. Combination of collagen and chitosan expected to be the best biomaterials as a wound dressing for the healing process. The study aimed to determine cytotoxicity assay on collagen-chitosan wound dressing sterilized by ultraviolet (UV) Light using Brine Shrimp Lethality Test (BSLT) method. The test groups were divided into K0, K1, K2, and K3 groups. K0 contained pure chitosan as a control group, K1 contained collagen 25%-chitosan 75%, K2 contained collagen 50%-chitosan 50%, K3 contained collagen 75%-Chitosan 25%. Collagen extracted from skin and scalp of snakehead fish (Channa striata) then mixed with chitosan until formed collagen-chitosan wound dressing. This study used Brine Shrimp Level Test (BSLT) method with solution concentration 10, 50, 100, 250, 500, 750 and 1000 ppm. Based on the results showed that K0, K1, K2, and K3 group had LC50 >1000 that proven collagen-chitosan wound dressing was non-toxic materials. As conclusion of the study explained composite wound dressing based on collagen-chitosan in all groups that sterilized under UV-Light along 15 minutes was not toxic based on Brine Shrimp Lethality Test showed LC50 >1000.
Co-Authors Ainiyah, Siti Dzurrotul Aisyah . Akhmad, Nur Faradila Alfri Danti Izky Oktavia Andreas Putro Ragil Santoso, Andreas Putro Ragil Angggraini, Rahayu Anggriyani, Rinda Anita Anita Anita Anita Anita Ardyarini Dyah Savitri Arsya Tazkiya Asror, Anita Rizki Rahmawati Atika Nur Laila Awwalia, Evy Sylvia Brina Thursina Dibiasi Budiarti, Rizqi Putri Nourma Chilyatuz Zahroh Devita Rahma Putri Deviyani, Dira Devyana Dyah Wulandari Edjianto, Soebagijo Poegoeh Endah Budi Permana Putri Endah Prayekti Endang Sulistiyani Ersalina Nidianti Evi Sylvia Awwalia Evy Sylvia Awwalia Fadillah Triasmoro Fadlillah Triasmoro Iskandar Fakurazi, Sharida Farah Nuriannisa Fauziah, Luqiana Fitria Agustina Gilang Nugraha Halimah Prisilia Hamiduumajid Ballihg Ballihgoo Hana Murni Kartika Sari Handayani Handayani Hermawati, Septina Isro’ Hidayat, Muhammad Taufiq Husein Firdaus Ibrahim Dwi Waluyo Putra Ima Kurniastuti Imratul Mukarramah Indah Lestari Indah Lestri Indah Nur Kamaliyah Irda Puspita Kamariyah, ST. Kardina, Rizki Nurmalya Khamida Khamida Khiliah Navis Khusnah, Hidayatul Kifli, Nurolaini Lili Susan Rahmawati Maghfuroh, Qonita Iklila Mardhotillah, Rachma Rizqina Marinda Dwi Puspitarini Mega Silvia Anggraini Sukri Moch Samsul Arifin Mohammad Nasir Mufarikoh, Zainatul Muhamad Damar Baskara Muhammad Afwan Romdloni Muhammad Raffli Muhammad Taufiq Hidayat Mukarramah, Imraatul Nathalya Dwi Kartika Sari Navis, Khiliah Nerisafitra, Paramitha Nila Qurrotul A’yun Nur Hamidah Paramitha Nerisafitra Prayekti, Endah Puspitasari, Adela Puspitasari, Renny Novi Rahayu Anggraini Rahmawati Fitri Awalia Rahmawati, Lili Susan Ratna Sofaria Munir Retno Diah Putri Ekayanti Rhomadhoni , Muslikha Nourma Riyan Sisiawan Putra Rizki Amalia Rizki Nurmalya Kardina Rizki Nurmalya Kardina Rohmah, Wardah Rukman, Nia Kurniaty Salimi, Syafrina Maulidyatus Salman Firmansyah Satya Nugraha Wirayudha Siska Wulandari, Siska Siti Dzurrotul Ainiyah Siti Husnul Khotimah Siti Nur Aniza Sofaria Munir, Ratna Soleha, Umdatus Sri Hartatik Suryandaru, Suryandaru Syafiuddin, Achmad Thomas Sumarsono Thomas Sumarsono Wandara Sekar Ayu Pramesti Wideistianti, Annisa Pricilya Widya Novita Sari Widya Novitasari Wijanarko, Robert Aditya Wulandari, Deyana Dyah Yauwan Tobing Lukiyono Zain, R.A Wafiqah Aulia Zainatul Mufarikoh