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All Journal Jurnal Biologi dan Pembelajarannya (JBP) Jurnal Sumberdaya Hayati Jurnal Pembelajaran dan Biologi Nukleus Konservasi Hayati Bioscientist : Jurnal Ilmiah Biologi PROFICIO: Jurnal Pengabdian Masyarakat Farmers: Journal of Community Services Maximus: Journal of Biological and Life Sciences TeknoKreatif: Jurnal Pengabdian kepada Masyarakat Indonesian Journal of Jamu
Mulyana, Jeane Siswitasari
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Agriculture, Biological Sciences & Forestry Humanities Biochemistry, Genetics & Molecular Biology Chemical Engineering, Chemistry & Bioengineering Chemistry Computer Science & IT Education Environmental Science Health Professions Immunology & microbiology Languange, Linguistic, Communication & Media Medicine & Pharmacology Social Sciences Other

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Quantitative Analysis of Phytochemical Compounds and Antihyperglycemic Potential of Robusta Coffee from West Lampung Khairani, Iffa Afiqa; Mulyana, Jeane Siswitasari; Olivia, Rahel Noviantika; Riana, Elisa Nurma; Anisa, Hida Arliani Nur
Jurnal Sumberdaya Hayati Vol. 10 No. 1 (2024): 2024
Publisher : Departemen Biologi, Institut Pertanian Bogor

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.29244/jsdh.10.1.1-6

Abstract

West Lampung Regency in Lampung Province produces high quality robusta coffee with a distinct, strong bitter flavour. Bitter flavour indicates the amount of bioactive substances produced by plants called phytochemicals. The aim of this study is to analyse the phytochemical compounds and antihyperglycemic potential of robusta coffee beans from West Lampung, Indonesia. Quantitative phytochemical analysis was carried out using the thin layer chromatography method and spectrophotometry. Animal experimental design using robusta coffee on hyperglycemia conditions in mice which was induced by alloxan 170 mg/kgBW subcutaneously and given by 1 ml of brewed robusta coffee for 10 days. The robusta coffee from West Lampung contained total caffeine 4,014.87 µg/g, total flavonoid content (TFC) 93.6 mg quercetin equivalent per gram of sample, with total alkaloid content (TAC) 0.848 mg of quinine equivalent per gram of sample, total tannin content (TTC) 182.3 mg of tannic acid equivalent per gram of sample, and total saponin content (TSC) 24.2 mg of quillaja bark equivalent per gram of sample. The administration of 1 ml/mice/day of robusta coffee brewed for 10 days did not show a decrease in blood sugar level in hyperglycemic mice, due to the short duration of the study, so the role of coffee in decreasing hyperglycemia conditions has not been optimally observed.
Aktivitas Penghambatan Bakteri Kitinolitik asal Limbah Pengolahan Udang Lampung terhadap Fusarium sp. dan Phytopthora sp. Suryanti, Erma; Chusniasih, Dewi; Mulyana, Jeane Siswitasari; Rahmah, Nadia; Mulyadi, Salsa Pratiwi; Istiadi, Khaerunissa Anbar
Jurnal Biologi dan Pembelajarannya (JB&P) Vol 12 No 2 (2025): OKTOBER 2025
Publisher : Universitas Nusantara PGRI Kediri

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.29407/jbp.v12i2.25907

Abstract

Organisme Pengganggu Tanaman (OPT) seperti Fusarium sp. dan Phytophthora sp. merupakan salah satu faktor yang menyebabkan penurunan produktivitas dan kegagalan panen tanaman hortikultura. Untuk mengatasi serangan OPT, petani melakukan pengendalian jamur patogen dengan menyemprotkan fungisida kimia. Namun, penggunaan fungisida sintesis/kimiawi yang tidak terkontrol dapat menyebabkan pencemaran serta resistensi pada OPT. Oleh sebab itu, diperlukan alternatif pengendalian yang lebih aman dengan biokontrol menggunakan bakteri kitinolitik. Bakteri kitinolitik akan mendegradasi kitin pada dinding sel jamur secara spesifik sehingga jamur tidak dapat berkembang dan mengalami kematian. Pada studi ini, dilakukan proses isolasi bakteri kitinolitik dari limbah unit pengolahan udang wilayah Lampung sehingga memiliki potensi penemuan bakteri kitinolitik dengan aktivitas degradasi kitin yang berbeda. Isolat bakteri kitinolitik potensial akan diujikan efektivitasnya sebagai antifungi patogen pada kapang model Fusarium sp. dan Phytophthora sp. secara in vitro. 12 isolat asal limbah pengolahan udang diuji aktivitas kitinolitiknya dengan aktivitas kitinolitik 0,37 – 19,7. Isolat bakteri diketahui mampu menghambat pertumbuhan fungi dengan indeks penghambatan tertinggi oleh isolat  C.68 sebesar 60,5% terhadap Fusarium sp. dan isolat S6.3 sebesar 72,68% terhadap Phytopthora sp.
Phytochemical Profile, Antioxidant Activity, and Hematological and Liver–Kidney Serum Markers of Lampung Green Robusta Coffee Extract in Lead-Induced Mice Khairani, Iffa Afiqa; Ananta, Jeni Putri; Firmansyah, Kiki; Anisa, Hida Arliani Nur; Riana, Elisa Nurma; Mulyana, Jeane Siswitasari; Fatriani, Rizka
Jurnal Jamu Indonesia Vol. 11 No. 2 (2026): Jurnal Jamu Indonesia
Publisher : Tropical Biopharmaca Research Center, IPB University

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.29244/jji.v11i2.472

Abstract

Lampung Province is one of Indonesia’s major robusta coffee–producing regions, and green coffee beans contain bioactive compounds with potential biological benefits. This study analyzed the phytochemical composition and antioxidant activity of ethanolic extract of Lampung green robusta coffee beans and evaluated its effects on hematological parameters and liver–kidney serum markers in lead acetate–induced mice. Phytochemical screening revealed alkaloids, flavonoids, phenolics, saponins, tannins, and terpenoids. Quantitative analysis showed total phenolics of 122.76 mg GAE/g extract, flavonoids of 12.23 mg QE/g extract, tannins of 208.86 mg TAE/g extract, and saponins of 1.32%. The extract exhibited very strong antioxidant activity (IC₅₀ = 21.99 ppm). Lead acetate exposure (20 mg/kg BW for 14 days) decreased erythrocyte counts and increased leukocyte and urea levels. Administration of the extract (400 mg/kg BW) improved erythrocyte counts, modulated leukocyte levels, and reduced urea concentrations.
Co-Authors Adhha, Nurul Ananta, Jeni Putri Andriyana, Ahmad Dodi ANDY DARMAWAN Anisa, Hida Arliani Nur Antonius Antonius Dewi Chusniasih DINA ANGGRAINI Fatriani, Rizka Firmansyah, Kiki Gres Maretta, Gres Iffa Afiqa Khairani Imaniar, Lisana Husna Khaerunissa Anbar Istiadi Luxiana, Eri Maulana, Muhammad Aqil Melinia, Nur Astri Mulyadi, Salsa Pratiwi Nadia Rahmah, Nadia Novriadi Novriadi, Novriadi Nur Hidayah Nurcahya, Gebriel Nurhayu, Winati Olivia, Rahel Noviantika Riana, Elisa Nurma Sari, Dian Anggria Sari, Mayang Rosi Dwi Sattia, Ana Suryanti, Erma Yanti Ariyanti, Yanti Zahra, Anisa Mumtaz