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Pengenalan Healthy Nutritious Food Knowledge dan Personal Hygiene di SD Negeri Gadingsari, Kecamatan Binakal, Kabupaten Bondowoso Puspito, Agung Nugroho; Ubaidillah, Mohammad; Widyawati, Arista; Nabyla, Cahyani Farhatin; Amami, Nurillah Adi Aurura; Herlambang, Yasina Alhadila Nazima
Jurnal Pengabdian Nasional (JPN) Indonesia Vol. 6 No. 2 (2025): Mei
Publisher : Lembaga Penelitian dan Pengabdian Kepada Masyarakat (LPPM) STMIK Indonesia Banda Aceh

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.63447/jpni.v6i2.1246

Abstract

Children's ability to eat healthy and nutritious food is closely related to education. Education is an inseparable process as an effort to grow and develop the potential in every human being. Especially in children, education plays an important role in forming a healthy diet and good hygiene behavior. However, in several studies in Indonesia, it is not uncommon to find that there is still a lack of knowledge about healthy and nutritious food and good hand washing among elementary school students which harms their health. As in SDN Gadingsari, Binakal District, there are still many elementary school students who have a low understanding of healthy and nutritious food. The author's purpose in carrying out this social project is to socialize, provide education and understanding directly to SDN Gadingsari students about the importance of eating healthy and nutritious food and good hygiene behavior through interactive learning such as showing videos as a result of eating nutritious healthy food, washing hands, providing material on the concept of the contents of my plate, the content in nutritious food such as carbohydrates, protein, fat, fiber, vitamins, and minerals, games to gymnastics together. The results showed that the students were very enthusiastic about learning about healthy and nutritious food. It is hoped that students can apply it in their daily lives.
ANALISIS SEGRAGASI GENOTIP BIOAKTIF DAN MORFOLOGI PADA TURUNAN F2 HASIL PERSILANGAN KETAN HITAM DAN CIHERANG Nugroho Puspito, Agung; Deviga Intan, Fia; Ubaidillah, Mohammad; Rhamadinda Nendra Tigara, Mellfani
Jurnal Penelitian Sains dan Teknologi Indonesia Vol 1 No 1 (2022): Jurnal Hasil Penelitian UNIVERSITAS JEMBER
Publisher : Lembaga Penelitian dan Pengabdian Kepada Masyarakat (LP2M) Universitas Jember

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.19184/jpsti.v1i1.47

Abstract

Tanaman padi dapat dikelompokkan menjadi 2 jenis, yaitu padi pigmented dan non pigmented. Salah satu contoh padi berpigmen yaitu padi beras merah dan padi beras hitam. Padi berpigmen dikenal sebagai sumber senyawa antioksidan termasuk flavonoid, antosianin, dan senyawa fenolik. Penelitian ini bertujuan untuk menganalisis segregasi hasil persilangan tanaman padi varietas Ketan Hitam dengan Ciherang melalui analisis karakter morfologi dan genotip bioaktif menggunakan marka molekuler SSR. Metode penelitian yang dilakukan yaitu dengan persiapan dan pemeliharaan tanaman (persiapan bahan tanam, pengolahan tanah, penanaman, pemupukan, pengairan, penyiangan, dan pemanenan) dan analisis genotip bioaktif (ekstraksi DNA, proses kerja PCR, elektroforesis dan pengamatan UV Transluminator). Analisis PCR menggunaan marka SSR dengan primer RM 346, RM 316, RM 228, dan RM 339. Analisis data yang digunakan adalah analisis distribusi frekuensi kuantitatif dengan panduan IRRI. Segragasi karakter morfologi pada populasi F2 hasil persilangan antara Ketan Hitam dan Ciherang masih tinggi, dimana dapat dilihat dari pola segragasi karakter pada tinggi tanaman, jumlah anakan, anakan produktif dan berat 1000 bijinya yang dikendalikan oleh banyak gen dan dipengaruhi oleh faktor lingkungan di dalamnya karena tidak memenuhi nisbah genetika mendel ataupun penyimpangannya. Bentuk morfologi hasil persilangannya lebih cenderung mirip dengan tetua betina atau Ketan Hitam. Sedangkan untuk hasil PCR dengan menggunakan marka SSR menunjukkan bahwa RM 346, RM 339 dan RM 228 dapat memvalidasi keseluruhan sampel yang digunakan baik tetua persilangan maupun F2 hasil persilangannya, sedangkan untuk RM 316 tidak dapat memvalidasi keseluruhan pita-pita DNA pada sampel penelitian.
EFFICIENT REGENERATION LOCAL BANANA TISSUE CULTURE USING FLORAL APICES BY CYTOKININ COMBINATION Makhsunah, Isnina Tasya; Habibi, Ali Mukit; Khofifa, Rendryana Aulia Nur; Ubaidillah, Mohammad
Jurnal Bioteknologi & Biosains Indonesia (JBBI) Vol. 10 No. 2 (2023)
Publisher : BRIN - Badan Riset dan Inovasi Nasional

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.55981/jbbi.2023.3123

Abstract

Banana (Musa sp.) is an important crop that is widely cultivated and consumed in Indonesia. Banana plants production can be increased through tissue culture with the use of explants and the right combination of hormones. This study was conducted to determine the use of flower apices explants on local banana varieties using a combination of cytokinin hormones containing BAP (Benzylaminopurine) and several levels of TDZ (Thidiazuron). The result of this research was analyzed using a one-way analysis of variance. The hormone combination in this study consists of MS + BAP 2 mg/L + three concentrations of TDZ (2 mg/L, 2.5 mg/L, and 3 mg/L) as a regeneration medium. Three local banana varieties (Kepok Putih, Kepok Kuning, and Pisang Susu) was cultured on the three combination medium. The results showed that the use of flower apices explants contained in banana flowers grown on MS media with a combination of cytokinin hormones could increase the efficiency of explant regeneration. Media MS + BAP 2 mg/L + TDZ 3 mg/L gave the best regeneration rate for Kepok Putih (62.50%), Kepok Kuning (62.50%) and Banana Susu (56.25%) compared to TDZ 2. mg/L and TDZ 3 mg/L. The novelty of this study can provide information in the propagation of local banana plants using flower apices explants.
Response of Rice Somatic Embryogenesis to Exogenous Melatonin About Its Role in Scavenging Reactive Oxygen Species Ubaidillah, Mohammad; Al Ayyubi, Nabila Nur Aisyah; Khofifa, Rendryana Aulia Nur; Dewanti, Parawita
AGRIVITA Journal of Agricultural Science Vol 46, No 1 (2024)
Publisher : Faculty of Agriculture University of Brawijaya in collaboration with PERAGI

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.17503/agrivita.v46i1.4060

Abstract

The success rate of explant morphogenesis in plant breeding using tissue culture techniques is frequently plagued by browning due to the oxidation of phenolic compounds. The cumulated amount of reactive oxygen species (ROS) drives the oxidation of phenolic compounds. Melatonin is reported to take a part in modulating the regulation of antioxidant gene expression, reducing the accumulation of reactive oxygen species, and enhancing the efficacy of tissue culture. This study aims to determine the optimal melatonin concentration on the efficiency of plantlet regeneration and expression of the antioxidant resistance gene in rice callus. This study utilizes rice TN1, Gogo Niti II, Ketan Hitam, and Cigeulis cultivars. Melatonin at 0, 10, and 15 µM concentrations is supplemented in plantlet regeneration media. Rice antioxidant-related genes, Mn-SOD, Cu/ZnSOD, Cytosolic APX, CAT, GPOD, OsAPX, and OsCATA, expressed after melatonin supplementation. Melatonin concentration at 10 µM generates the highest expression of all tested genes in TN1 compared to other varieties. The cumulated amount of hydrogen peroxide (H2O2) shows that Melatonin has the potential to increase the proportion of plant regeneration in Cigeulis (90.48%) and Ketan Hitam (91.67%) varieties with a concentration of 10 µM and in TN1 (94.44%) and Gogo Niti II (80%) at a concentration of 15 µM.
Morphological, Physiological and Molecular Characteristics of Tolerant Sugarcane to Waterlogging Stress Wibisono, Viki Bayu; Avivi, Sholeh; Ubaidillah, Mohammad; Sri Hartatik
Jurnal Agronomi Indonesia (Indonesian Journal of Agronomy) Vol. 50 No. 2 (2022): Jurnal Agronomi Indonesia
Publisher : Indonesia Society of Agronomy (PERAGI) and Department of Agronomy and Horticulture, Faculty of Agriculture, IPB University, Bogor, Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (726.434 KB) | DOI: 10.24831/jai.v50i2.40760

Abstract

Cekaman genangan menyebabkan penurunan hasil 15-45% pada tanaman tebu. Penelitian ini bertujuan untuk mengetahui karakteristik morfologi, fisiologi, dan molekuler tanaman tebu toleran terhadap cekaman genangan. Percobaan dilaksanakan di Laboratorium Program Studi Agroteknologi, Fakultas Pertanian, Universitas Jember, Jawa Timur pada bulan September 2021-Februari 2022. Rancangan percobaan menggunakan rancangan acak kelompok (RAK) faktorial. Faktor pertama yaitu genotipe tebu dengan enam genotipe (Bululawang, PS 862, Cening, PS 881, M3, M4). Faktor kedua yaitu periode penggenangan dengan tiga taraf (30 hari, 60 hari, dan 90 hari). Faktor ketiga yaitu perlakuan cekaman genangan (tanpa cekaman genangan dan dengan cekaman genangan). Variabel pengamatan meliputi tinggi tanaman, luas permukaan daun, diameter batang, bobot segar akar, jaringan aerenkim, klorofil total, kerapatan stomata, kandungan H2O2 dan ekspresi gen antioksidan. Hasil penelitian menunjukkan bahwa cekaman genangan berdampak pada pembentukan jaringan aerenkim, penurunan bobot segar akar, perubahan tinggi tanaman, penurunan luas area daun, penurunan klorofil total, penurunan kerapatan stomata, peningkatan kandungan hidrogen peroksida dan ekspresi gen antioksidan. Genotipe Cening menunjukkan respon persentase penurunan yang rendah dibandingkan dengan kontrol tanpa cekaman pada variabel morfologi, fisiologis dan ekspresi gen antioksidan yang lebih tinggi. Kata kunci: antioksidan, banjir, ketahanan, reactive oxygen species, Cening
Karakterisasi Morfologi dan Molekuler Galur Padi Introduksi Golden Rice F8 terhadap Penyakit Blas dan Hawar Daun Bakteri Mai Abdul Imam Buqori, Dwi; Agus Dyanshah, Danny; Hartatik, Sri; Bayu Wibisono, Viki; Ubaidillah, Mohammad
Jurnal Agronomi Indonesia (Indonesian Journal of Agronomy) Vol. 50 No. 3 (2022): Jurnal Agronomi Indonesia
Publisher : Indonesia Society of Agronomy (PERAGI) and Department of Agronomy and Horticulture, Faculty of Agriculture, IPB University, Bogor, Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (563.893 KB) | DOI: 10.24831/jai.v50i3.41305

Abstract

Golden rice merupakan padi yang di dalam endosperma bijinya memiliki kandungan β-karoten atau provitamin A. Beberapa upaya telah dilakukan untuk meningkatkan ketahanannya terhadap penyakit blas dan hawar daun bakteri (HDB). Hasil pemuliaan Golden rice telah mendapatkan turunan ke 8 (F8) yang bersifat stabil. Penelitian ini bertujuan untuk mengkarakterisasi agromorfologi dan genotipe galur padi introduksi Golden rice F8 PAC Nagdong/IR36/IR64. Penelitian dilakukan di Laboratorium Agroteknologi, Fakultas Pertanian, Universitas Jember. Benih yang digunakan yaitu tiga galur padi intorduksi Golden rice F8 PAC Nagdong/IR36/IR64 dengan kode 302/IR-2-2(6)/1, 302/IR-2-2(7)/2, 302/IR-2-2(8)/1 serta varietas IR36 dan IR64 sebagai tanaman kontrol. Analisis genotipe meliputi gen Pik-s, Pi54, Pi-ta, Xa4 dan Xa7. Hasil penelitian menunjukkan bahwa karakter agromorfologi pada seluruh galur Golden rice untuk karakter tinggi tanaman, jumlah anakan dan jumlah anakan produktif tidak berbeda nyata. Tinggi tanaman Golden rice termasuk dalam kategori semidwarf, jumlah anakan >24.6 dengan anakan produktif mencapai lebih dari 85%, panjang malai antara 20.09-20.85 cm, dan bobot per rumpun mencapai 42.94 g serta seluruh galur Golden rice memiliki gen pengkode sifat tahan terhadap penyakit blas dan HDB. Kata kunci: β-karoten, galur, HDB, introduksi, marka
Pengaruh Melatonin Eksogen terhadap Performa dan Perkembangan Organ Generatif Tanaman Okra (Abelmoschus esculentus L.) Rohman, M Andik Fathur; Hartatik, Sri; Buqori, Dwi Mai Abdul Imam; Puspito, Agung Nugroho; Ubaidillah, Mohammad
Jurnal Agronomi Indonesia (Indonesian Journal of Agronomy) Vol. 50 No. 3 (2022): Jurnal Agronomi Indonesia
Publisher : Indonesia Society of Agronomy (PERAGI) and Department of Agronomy and Horticulture, Faculty of Agriculture, IPB University, Bogor, Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (948.223 KB) | DOI: 10.24831/jai.v50i3.42121

Abstract

Buah okra kaya akan manfaat yang berguna memenuhi nutrisi yang dibutuhkan oleh tubuh. Melatonin merupakan biomolekul pleiotropik alami yang ditemukan secara luas pada tumbuhan maupun hewan untuk meningkatkan hasil, pembungaan, pembentukan dan perkembangan buah, partenokarpi, dan ripening. Penelitian bertujuan mengetahui respon melatonin eksogen terhadap performa dan perkembangan organ generatif tanaman okra. Penelitian dilakukan pada Desember 2021-Februari 2022 di lahan okra PT. Gading Mas Indonesia Teguh, Ajung, Jember, Indonesia. Pemberian melatonin dilakukan secara eksogen dengan menyemprotkan ke semua bagian tanaman okra yang sudah masuk fase generatif. Percobaan dilakukan dengan satu faktor konsentrasi melatonin dengan empat taraf yaitu 0 µM, 200 µM, 350 µM, dan 500 µM. Kombinasi perlakuan dilakukan pengulangan sebanyak lima kali sehingga didapatkan 20 satuan percobaan. Hasil penelitian menunjukkan melatonin berpengaruh terhadap performa dan kualitas tanaman okra. Konsentrasi 350 µM menghasilkan rata-rata jumlah daun tertinggi 14.8 daun. Konsentrasi 500 µM menghasilkan waktu gugur kelopak paling lama dengan rata-rata 4.8 hari dan persentase keberhasilan pembentukan buah sebesar 100 %. Variabel jumlah biji paling tinggi pada perlakuan kontrol dengan rata-rata 57.2 biji dan pematangan buah atau ripening paling lama pada konsentrasi 500 µM dengan rata-rata 33.6 hari. Melatonin tidak berpengaruh nyata terhadap panjang buah dan diameter buah okra. Kata kunci: hormon, partenokarpi, ripening, sayuran
Flower bagging affects seed quality and dormancy period in Na-Oogst tobacco (Nicotiana tabacum L. var H-382) Thamrin, Nurhaliza; Amilin, Miftahul; Mufadilah, Mitha Aprilia; Ubaidillah, Mohammad
Jurnal Agronomi Indonesia (Indonesian Journal of Agronomy) Vol. 51 No. 2 (2023): Jurnal Agronomi Indonesia (Indonesian Journal of Agronomy)
Publisher : Indonesia Society of Agronomy (PERAGI) and Department of Agronomy and Horticulture, Faculty of Agriculture, IPB University, Bogor, Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.24831/ija.v51i2.45493

Abstract

Seeds of tobacco plants are unable to germinate immediately after harvest because they have a dormancy period. The seed dormancy period may be shortened by bagging methods to affect the hormone content in the seeds. The bagging flower makes the dark conditions in the process of flowering and seed development, affecting the content of the hormone’s auxin and ABA. This study aimed to determine the effects of different bag colors on the dormancy period of tobacco seeds as evidenced by their vigor and viability. The research was conducted from March to August 2022 at PT. Dwi Light Tobacco Green House, Lombok Wetan, Bondowoso, Indonesia. Four crepe colors of paper were used as bagging treatments (black, red, yellow, blue) and control (without bagging) and replicated four times. The results showed that the bagging treatment significantly affected one hundred-seed weight, seed germination, seed growth rate, and seedling height. Black crepe paper resulted in the highest seed germination percentage (98.5%) and seed growth rate (11.68). Keywords: bag color, seed germination, viability, vigor
OPTIMIZATION OF PRIMARY SOMATIC EMBRYOGENESIS INDUCTION OFCOCOA (Theobroma cacaoL.) THROUGH THE ADDITION OF PHENOLICCOMPOUNDSUPPRESSANT MATERIALS Priandani, Putri Oktaviana; Pancaningtyas, Sulistyani; Ubaidillah, Mohammad
Jurnal Bioteknologi & Biosains Indonesia (JBBI) Vol. 10 No. 1 (2023)
Publisher : BRIN - Badan Riset dan Inovasi Nasional

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.55981/jbbi.2023.1701

Abstract

Somatic embryogenesis is a propagation technique in which explants form somatic embryos. The percentage of primary somatic embryo formation in cocoa tends to be low due to the significant secretion of phenolic components in flower explants, thereby reducing the conversion rate of explants into embryos. This is indicated by browning that occurs in explants or the culture medium. The purpose of this study was to optimize primary somatic embryogenesis induction in cocoa through the addition of several types of phenolic compound suppressor materials to the culture medium. Staminodia explants were grownon DKW medium with factorial treatment: various phenolic compound suppressing materials (control; 300 mg L-1PVP; 15 mg L-1silver nitrate; 12 mg L-1melatonin; 300 mg L-1ascorbic acid) and cocoa cultivars (MCC-02 and ICCRI-09). The results showed that each material treatment produced a different response in suppressing phenolic compounds in each cultivar. The melatonin treatment resulted in the lowest percentage of browning intensity, which also had the best impact on morphology, callus size, wet weight,and the percentage of live explants.
SEGREGATION ANALYSIS OF MORPHOLOGICAL CHARACTER AND BIOACTIVE GENOTYPE OF RICE PLANT F3 (CEMPO SALAMET/IR64) POPULATION Ubaidillah, Mohammad
Jurnal Bioteknologi & Biosains Indonesia (JBBI) Vol. 9 No. 1 (2022)
Publisher : BRIN - Badan Riset dan Inovasi Nasional

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.55981/jbbi.2022.1783

Abstract

Red rice contains high anthocyanin and bioactive antioxidant compounds that prevent free radical reactions. Cempo Salamet has potential as an antioxidant source, and the characteristics are red colored grains, 4–5 months old, 169 cm plant height, 7 productive tillers per plant, and resistance to blast disease. IR64 had been developed with the following characteristics: 3 months old, 85 cm plant height, 20–35 productive tillers per plant, resistance to brown leafhoppers pigment. This study aimed to obtain information on the segregation of the F3 population from crosses between the Cempo Salamet and IR64 varieties. Research methods included preparation and maintenance with genotype analysis. PCR analysis was conducted using SSR markers with primer RM346, RM316, RM228, and RM339. The segregation in F3 plants was 50% for >130 cm plant height, 51% for 10–19 tillers per plant, 67% for 2.2 g/100-grain weight, and 33% strong red for colour intensity. The findings demonstrated that SSR markers RM346, RM339, and RM228 could validate Cempo Salamet, IR64, and F3 DNA bands. However, RM316 could not validate all DNA bands in the research sample.
Co-Authors Adhitya Wardhono Adinda Putri Maharani Affiana, Alfina Nur Agus Dyanshah, Danny Al Ayyubi, Nabila Nur Aisyah Aliyah, Rachmah Amami, Nurillah Adi Aurura Amelia, Wirda Fransisca Amilin, Miftahul Andini, Azizah Dian Anggira, Desi Aprilia, Sofi Arinanta, Arya Arniyanti, Muslimah Ashilia, Indah Aulia, Isthifa Annisa Aurelya, Lalyta Az-zahro, Aulia Fatimah Azmi, Fatihah Ulul Azzahra, Ajeng Putri Bayu Aprillianto Bayu Wibisono, Viki Buqori, Dwi Mai Abdul Imam Cahyani, Ardhya Pramisti Regita Candra Prayoga, Mohammad Choirunnisa, Eviyanti Deviga Intan, Fia Dewi Kusuma Wardani Dewi, Ruli Kusuma Dyanshah, Danny Agus Faiqah, Qazzalva Nidya Faizyah, Nadia Nurdiyana Fatmawati, Yeni Fauziah, Rafika Febriani, Nawal Aprodita Febriyanti, Dwi Aidil Ferdyansyah, Dandy Firdaus, Rayhan Wahyu Habibi, Ali Mukit Hapsari, Salu Silvi Hartita, Margaretha Putri Harun, Anindita Firma Aulia Herlambang, Yasina Alhadila Nazima Hidayat, Adrian Shevchenko Hoiriyah, Alfiatin Imelda, Laela Vita Intansari, Herlindha Angghelinna Devfi Isnainun, Ainaya Jamila, Raudhotun Jayanti, Silvi Dwi Karyaningtyas, Setyowati Kaylani, Gyzna Luzetta Khofifa, Rendryana Aulia Nur Kim, Kyung Min Kirana, Zakiyah Galuh Pusphita Candra Kristianawati, Fany Indah Kumala, Velliana Dhea Mai Abdul Imam Buqori, Dwi Makhsunah, Isnina Tasya Marvintha, Kirana Ndaru Maulina, Presilia Eka Ma’arif, Samsul Misi, Dina Aulia Mubarakah, Lailatul Mufadilah, Mitha Aprilia Nabila, Nailun Nabyla, Cahyani Farhatin Nailah, Nurun Nasir, M Abd Nayyiroh, Dini Anjum Milatin Novita, Septian Abelia Nugroho Puspito, Agung Nugroho, Alfin Nur Hasanah Nur, Sefi Anindia Tiyana NURUL HIDAYAH Parawita Dewanti Prakoso, Bhim Prasetyaningrum, Bintang Dwi Priandani, Putri Oktaviana Puspito, Agung Nugroho Putra, Iqbal Firlana Putri, Aulia Meuthia Putri, Duety Nectarinia Putri, Revita Salsabila Qori’ah, Ciplis Gema Rachma P. A, Shabrina Rachmandhika, Yusuf Rafa, Muhammad Ramadhani, Vian Restanto, Didik Pudji Rhamadinda Nendra Tigara, Mellfani Rohman, M Andik Fathur Rozzita, Noor Rusdi, Nabila Maulida Salsabila, Ayu Saputra, Martha Alit Saputro Gumelar, Bachtiar Sari, Sasmita Sarifudin, Ahmat Setiyono Setiyono Shafany, Astry Sholeh Avivi Sifah, Isrina Sinulingga, Trisna Handayani BR Siswanto, Siti Mazzhidha Rhaumi Novia Sitompul, Novrida Yanti Sofwah, Ayaa Sri Hartatik Sri Hartatik Sukaesih, Nabilah Shintia Sulistyani Pancaningtyas Sultonia, Ivone Suryalaksono, Kemal Beryl Suud, Hasbi Mubarak Thamrin , Nurhaliza Thamrin, Nurhaliza Tri Agus Siswoyo TRI HANDOYO Wahyu, Dahlia Tiara Trisnaning Wardan, Nuril Maulidiyah Wibisono, Viki Bayu Widyawati, Arista Wijayanti, Karmila Dwi Zahrosa, Dimas Bastara Zakaria, Moch Faesal septean Zaphora, Shineya Zennita