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Patogenitas dan Pengendalian Infeksi Vibrio sp pada Udang Vaname (Litopenaeus vannamei) Menggunakan Ekstrak Daun Miana (Coleus scutellarioides (L) Benth) Buana Basir; Halimah Halimah; Kariyanti Kariyanti
Musamus Fisheries and Marine Journal Vol 5 No 2 (2023): Musamus Fisheries and Marine Journal
Publisher : Faculty of Agriculture Musamus University

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.35724/mfmj.v5i2.5458

Abstract

Penyakit menjadi kendala utama karena dapat menimbulkan angka kematian yang relatif tinggi. Salah satu jenis penyakit yang dapat menyebabkan kematian massal pada udang adalah vibriosis. Pengendalian penyakit telah dilakukan oleh para petani dengan menggunakan antibiotik. Namun hal ini berdampak pada resistensi dan residu pada udang. Oleh karena itu diperlukan pengobatan alternatif yang bersifat alami dan lebih ramah lingkungan. Penelitian ini bertujuan untuk mengetahui kinerja daun miana lam dalam mengobati infeksi penyakit Vibrio sp pada udang vannamei. Penelitian ini dilaksanakan pada bulan Juni-Juli 2020 yang bertempat di Laboratorium Peternakan Universitas Hasanuddin. Persiapan bakteri dilakukan di Laboratorium Kesehatan Ikan dan Lingkungan BPBAP (Balai Budidaya Air Payau) Takalar. Metode penelitian dirancang menggunakan 3 perlakuan dan 3 kali ulangan dengan konsentrasi ekstrak daun miana 1000 µg/mL. Udang terkena V. alginolyticus. dengan kepadatan bakteri 103, 105, dan 107 CFU/mL. Data dianalisis secara deskriptif dengan membandingkan kondisi udang yang sehat dan yang sakit. Hasil penelitian menunjukkan bahwa udang yang terinfeksi V. alginolyticus sembuh setelah udang direndam dengan ekstrak daun miana sebanyak empat kali. Kesimpulannya penggunaan ekstrak daun miana dapat menjadi alternatif pengobatan udang yang menyembuhkan V. alginolyticus. alginolyticus dipulihkan setelah udang direndam dengan ekstrak daun miana sebanyak empat kali. Kesimpulannya penggunaan ekstrak daun miana dapat menjadi alternatif pengobatan udang yang menyembuhkan V. alginolyticus. alginolyticus dipulihkan setelah udang direndam dengan ekstrak daun miana sebanyak empat kali. Kesimpulannya penggunaan ekstrak daun miana dapat menjadi alternatif pengobatan udang yang menyembuhkan V. alginolyticus.
Toksisitas Daun Miana (Coleus scutellarioides (L) Benth) Sebagai Antibakteri Pada Udang Vannamei (Litopenaeus vannamei) Basir, Buana; Isnansetyo, Alim; Istiqomah, Indah; Jabbar, Firmansyah Bin Abd
SIGANUS: Journal of Fisheries and Marine Science Vol 1 No 2 (2020): SIGANUS: Journal of Fisheries and Marine Science
Publisher : Universitas Sulawesi Barat

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.31605/siganus.v1i2.653

Abstract

Tujuan Penelitian ini untuk menganalisis toksisitas daun miana sebagai antibakteri pada udang vanamei. Penelitian ini dilaksanakan pada Maret sampai Oktober 2019, di Laboratorium Sekolah Tinggi Teknologi Kelautan (STITEK) Makassar. Sampel daun miana berasal dari beberapa daerah di Sulawesi Selatan. Uji aktivitas antibakteri ekstrak daun miana dengan tiga fraksi, yaitu ethanol, n-hexane, dan chloroform menggunakan metode difusi kertas cakram dengan konsentrasi ekstrak 500 µg/mL dan 1000 µg/mL, sebanyak tiga kali ulangan. Uji toksisitas metode BST (LC70) dengan konsentrasi uji 2500, 1250, 625, 312.5, 156.25, 78.125 µg/mL. Data yang diperoleh dianalisis secara deskriptif. Hasil penelitian menunjukkan bahwa uji aktivitas ekstrak daun miana tertinggi pada fraksi chloroform, dengan diameter hambat 28 mm. Sementara pada antibiotik oxytetracycline diameter hambat sebesar 31 mm. Nilai toksisitas pada tingkat mortalitas 30% pada konsentrasi 1250 µg/mL. Ekstrak daun miana fraksi chloroform mampu menggantikan peran antibiotic oxytetracycline dengan penggunaan konsentrasi dibawah 1250 µg/mL.
In Vitro Anti-Vibrio Activity of Miana Leaves (Coleus scutellarioides (L) Benth) Basir, Buana; Alim Isnansetyo; Indah Istiqomah
JFMR (Journal of Fisheries and Marine Research) Vol. 8 No. 1 (2024): JFMR on March
Publisher : Faculty of Fisheries and Marine Science, Brawijaya University, Malang, Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.21776/ub.jfmr.2024.008.01.7

Abstract

The aim of this research is to determine the bioactive compounds of miana leaves of Coleus scutellarioides (L) Benth against Vibrio spp.. This research was carried out from April to August 2018 at the Fish Pests and Diseases Laboratory, Department of Fisheries, Gadjah Mada University. Miana leaf samples were dried at room temperature, macerated with ethanol solvent and tested for antibacterial activity. Separation of bioactive compounds was carried out using liquid partition and silica gel using the column chromatography method. MIC (minimum inhibitory concentration) is applied to evaluate antibacterial activity. The results showed that the highest antibacterial activity was found in the chloroform fraction with inhibitory zone diameters for V. parahaemolitycus, V. alginolitycus, and V. harveyii of 25 mm, 26 mm, and 20 mm at a dose of 20 µg/disk. These findings indicate that the antibacterial activity of miana leaf crude extract is comparable to oxytetracycline with inhibition zones of 25 mm, 31 mm and 20 mm respectively against similar bacterial strains at 20 µg/disk. Therefore, miana leaves are a prospective natural source of anti-vibrio compounds.
Survival rate of tiger shrimp (Penaeus monodon) pre and post infected with White Spot Syndrome Virus (WSSV) fed with the addition of miana leaf extract (Coleus scutellarioides) Febriani, Keky; Basir, Buana; Heriansah, Heriansah
Akuatikisle: Jurnal Akuakultur, Pesisir dan Pulau-Pulau Kecil Vol 7, No 2 (2023)
Publisher : Sangia Research Media and Publishing

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.29239/j.akuatikisle.7.2.151-158

Abstract

The plant species of miana (Coleus scutellariodes) has been historically recognised for its antiviral properties.  However, there is currently no scientific evidence supporting the efficacy of miana leaf extract in preventing White Spot Syndrome Virus (WSSV) in tiger prawns (Penaeus monodon) when administered via feed. This study aimed to determine the impact of including miana leaf extract in the diet on the  survival percentage of tiger prawns that have been infected with the WSSV. The study was carried out between June and August 2023 in the Laboratory of Parasite and Fish Disease and the Hatchery Laboratory of Hasanuddin University. This study employed a completely randomized experimental design consisting of five distinct treatments, each with three replicates. The experimental treatments included the control group (treatment A), where food was provided without the inclusion of miana leaf extract. Furthermore, there were four treatment groups (treatments B, C, D and E) where feed was supplemented with miana leaf extract at concentrations of 125, 250, 500, and 750 µg mg-1 feed, respectively. Rearing was performed in two phases: preinfection (25 days) and post- infection (7 days). The statistical results of the statistical on pre-WSSV infection indicated that the addition of miana leaf extract to the food resulted in survival that was not significantly different (p>0.05). The results were different post-infection, significantly (p<0.05) the shrimp fed with the addition of miana leaf extract were higher than those without the extract. Furthermore, there was a significant difference in shrimp survival between pre- and post-WSSV infection. WSSV mitigation through immunostimulants using miana leaf extract, which is inexpensive and environmentally friendly, has the potential to minimize the impact of WSSV infection.
PENGEMBANGAN KEWIRAUSAHAAN BERKELANJUTAN DAN TERPADU DI STITEK BALIK DIWA Basir, Buana; Kabangnga, Arnold; Saokani, Jawiana; Hidayani, Mesalina Tri; Hamsia, .
Jurnal Dinamika Pengabdian Vol. 6 No. 1 (2020): JURNAL DINAMIKA PENGABDIAN VOL. 6 NO. 1 OKTOBER 2020
Publisher : Departemen Budidaya Pertanian Fakultas Pertanian UNHAS

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20956/jdp.v6i1.7628

Abstract

The underdeveloped entrepreneurial condition at the Balik Diwa Marine Technology College is suspected to be due to the limited business facilities. Moreover, the student mindset is not focused on the benefits of sustainable business independence. High entrepreneurial motivation is defeated by the desire of students to immediately graduate and get a certificate to find work in various agencies. The solution presented by higher education institutions and the Ministry of Research, Technology and Higher Education is to motivate students about the benefits of doing independent and sustainable business activities, by rolling out several student activity programs such as the Student entrepreneurship program, village development grant programs, student creativity programs, integrated learning and work programs, and programs. Entrepreneurial development. The entrepreneurship development program (PPK) is a program that is temporarily run by the Balik Diwa Makassar high school of marine technology which involves students and alumni as tenants. The method used in fostering tenants is through entrepreneurship training, comparative studies, and apprenticeships to small and medium-sized companies, assistance in starting and developing businesses according to their respective competencies, talents, and interests. After going through the selection, training, comparative study, and apprenticeship stages, Tenan seemed to be more enthusiastic with high enthusiasm in starting their respective businesses. There are 12 types of businesses formed from 20 tenants, and more than five types of businesses that have worked from upstream to downstream, starting from the process of pioneering and producing products to marketing. Several types of business, namely Herbal Probiotic (PH) Jolly, Saline to Fresh Ornamental Fish, aquaponic catfish enlargement, crab shell chitosan, seaweed lotion, seaweed crackers /sticks, and LACUBA fishing aid.   Keywords: Entrepreneurship program, tenants, business, students.   ABSTRAK Kondisi kewirausahaan yang kurang berkembang di Sekolah Tinggi Teknologi Kelautan Balik Diwa diduga karena masih terbatasnya fasilitas usaha dan pola pikir mahasiswa yang kurang terarah terhadap manfaat dari kemandirian usaha yang berkelanjutan. Motivasi wirausaha yang tinggi terkalahkan oleh keinginan mahasiswa untuk segera lulus dan mendapatkan ijazah buat mencari pekerjaan di berbagai instansi. Solusi yang dihadirkan oleh perguruan tinggi dan Kemristek Dikti adalah memberi motivasi kepada mahasiswa tentang manfaat melakukan kegiatan usaha mandiri dan berkelanjutan, dengan menggulirkan beberapa program kegiatan mahasiswa seperti program kewirausahaan mahasiswa, program hibah bina desa, program kreativitas mahasiswa, program belajar dan bekerja terpadu, dan program pengembangan kewirausahaan. Program pengembangan kewirausahaan (PPK) merupakan program yang sementara dijalankan oleh Sekolah Tinggi Teknologi Kelautan Balik Diwa Makassar yang melibatkan mahasiswa dan alumni sebagai tenan. Metode yang digunakan dalam membina tenan yaitu melalui pelatihan kewirausahaan, studi banding dan magang ke perusahaan kecil dan menengah, pendampingan dalam merintis dan mengembangkan usaha sesuai dengan kompetensi, bakat dan minat masing-masing. Setelah melalui tahap seleksi, pelatihan, studi banding dan magang, tenan terlihat lebih antusias dengan semangat yang tinggi dalam merintis usahanya masing-masing. Usaha yang terbentuk sebanyak 12 jenis dari 20 orang tenan, dan lebih dari lima jenis usaha yang sudah berkiprah dari hulu hingga hilir, mulai dari proses merintis dan menghasilkan produk hingga sampai ke pemasaran sudah dilakukan. Beberapa jenis usaha tersebut, yaitu Probiotik Herbal (PH) Jolly, Ikan Hias salin ke tawar, pembesaran lele akuaponik, Kitosan cangkang rajungan, lotion rumput laut, krupuk/stik rumput laut, dan alat bantu tangkap ikan LACUBA.   Kata kunci: Program kewirausahaan, tenan, usaha, mahasiswa.
Effect of Coleus blumei Miana Leaf Extract in Feed on Whiteleg Shrimp Immune Response Basir, Buana; Jum’ah, Ulfatul; Rapi, Nuraeni Lewa; Kustam, Sri Rukmini
Jurnal Perikanan Universitas Gadjah Mada Vol 27, No 1 (2025)
Publisher : Universitas Gadjah Mada

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.22146/jfs.106891

Abstract

Coleus blumei (miana) can be used as an antimicrobial, antibacterial, antioxidant, and anti-inflammatory. Research on miana leaf extract in feed to increase the immune response of whiteleg shrimp has not been found. This study aims to analyze miana leaf extract in feed on the immune response of whiteleg shrimp. The study was conducted in July 2023 at the Hasanuddin University Laboratory and the Bogor Agricultural Institute Laboratory. The method used was a completely randomized design, 4 treatments and 3 replications, extracts of 0 g, 1 g, 2.5 g, and 7.5 g/kg of feed. The statistical results of the treatment gave a significant effect (p<0.05) on total hemocytes and phenoloxidase activity but did not give a significant effect (p>0.05) on the survival of whiteleg shrimp. The extract of 7.5 g/kg of feed had the highest impact on total hemocytes 8.43x103 cells/mm3, phenoloxidase activity 0.222 optical density ƛ=490, and 100% survival. The extract of 0 g/kg feed had the lowest impact on total hemocytes, 2.37x103 cells/mm3, phenoloxidase activity of 0.09 Optical Density ƛ=490, and survival of 90%. Conclusion, Coleus blumei leaf extract in feed can improve the immune response and survival of whiteleg shrimp. The recommended concentration in aquaculture is 7.5 g/kg.
Utilization of skipjack tuna Katsuwonus pelamis offal waste for mass culture of Daphnia sp. as feed for Betta sp. Cahyono, Indra; Mutmainah, Sri Nurul; Basir, Buana; Mulyawan, Ardi Eko; Mulyani, Sri
Jurnal Akuakultur Indonesia Vol. 24 No. 2 (2025): Jurnal Akuakultur Indonesia
Publisher : Indonesian Society of Scientific Aquaculture (ISSA)

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.19027/jai.24.2.233-243

Abstract

This study aims to assess the impact of skipjack tuna (Katsuwonus pelamis) offal soaking water on the mass culture of Daphnia sp. for the growth of Betta fish (Betta sp.). This study was conducted for 30 days with treatment of offal 4.5 ml/L (treatment B), 7.5 ml/L (treatment C), 10.5 ml/L (treatment D) and commercial feed without skipjack offal soaking water as control (treatment A). The variables data of this study consisted of growth rate and population density of Daphnia sp., which were analyzed using variance analysis. The results showed that the highest population growth rate was found in the treatment of offal soaking water 4.5 ml/L with a value of 11.59% and a density of 260 individuals/liter on the 19th day of rearing. Daphnia sp. produced from soaking tuna offal can also be used as a natural food for the growth and development of Betta fish, although its effectiveness is still lower than other organic materials. Keywords: Daphnia sp., growth, mass culture, skipjack offal ABSTRAK Penelitian ini bertujuan untuk mengetahui pengaruh air rendaman jeroan ikan cakalang (Katsuwonus pelamis) pada kultur massal Daphnia sp. terhadap pertumbuhan ikan Betta (Betta sp.). Penelitian ini dilakukan selama 30 hari dengan perlakuan air rendaman jeroan ikan cakalang sebanyak 4,5 ml/L (perlakuan B), 7,5 ml/L (perlakuan C), 10,5 ml/L (perlakuan D) dan pakan komersil tanpa air rendaman jeroan ikan cakalang sebagai kontrol (perlakuan A). Data variabel penelitian terdiri dari laju pertumbuhan dan kepadatan populasi Daphnia sp. dianalisis menggunakan analisis ragam. Hasil penelitian menunjukkan bahwa laju pertumbuhan populasi tertinggi pada perlakuan menggunakan air rendaman jeroan 4,5 ml/L dengan nilai 11,59% dan dengan kepadatan individu 260 individu/liter pada hari pemeliharaan ke 19. Daphnia sp. yang dihasilkan dari perendaman jeroan ikan cakalang juga dapat digunakan sebagai pakan alami untuk pertumbuhan dan perkembangan ikan Betta (Betta sp.) walaupun efektivitasnya masih lebih rendah dibandingkan bahan organik lainnya. Kata kunci: Daphnia sp., jeroan cakalang, kultur massal, pertumbuhan
EFEKTIVITAS PENGGUNAAN DAUN KELOR SEBAGAI BAHAN BAKU PAKAN IKAN NILA (Oreochromis niloticus) Basir, Buana; Nursyahran, Nursyahran
OCTOPUS: JURNAL ILMU PERIKANAN Vol. 7 No. 2 (2018): OCTOPUS
Publisher : Universitas Muhammadiyah Makassar

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.26618/octopus.v7i2.2466

Abstract

Penelitian ini bertujuan menganalisis efektivitas penggunaan daun kelor sebagai bahan baku pakan ikan nila (Oreochromis niloticus). Penelitian dilakukan selama 40 hari, menggunakan metode eksperimen dengan Rancangan Acak Lengkap (RAL), empat perlakuan dan tiga kali ulangan. Perlakuan A sebagai kontrol tanpa penggunaan daun kelor (0%). Perlakuan B dengan penggunaan daun kelor sebesar 10%. Perlakuan C dengan penggunaan daun kelor sebesar 15 %, dan perlakuan D dengan penggunaan daun kelor sebesar 20%. Penelitian dilaksanakan pada bulan April sampai dengan Mei Tahun 2017. Hasil penelitian menunjukkan bahwa penggunaan daun kelor sebagai bahan baku pada pakan sebesar 20% (perlakuan D) menghasilkan pertumbuhan bobot tertinggi pada ikan nila (Oreochromis niloticus), dengan nilai sintasan tertinggi pada penggunaan tepung daun kelor sebesar 15 % (perlakuan C). Penggunaan daun kelor sebagai bahan baku pada pakan efektif meningkatkan bobot pertumbuhan ikan nila (Oreochromis niloticus) dengan dosis 20% di dalam pakan.
Effect of chitosan from crab shells (Portunus pelagicus) on the chemical properties of liquid fertilizer Putri, Try Amelia; Harianti, Harianti; Basir, Buana; Khairiyah, Zul
Akuatikisle: Jurnal Akuakultur, Pesisir dan Pulau-Pulau Kecil Vol 9, No 2 (2025)
Publisher : Sangia Research Media and Publishing

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.29239/j.akuatikisle.9.2.123-126

Abstract

Swimming crab shell waste is a fishery byproduct that has the potential to pollute the environment if not utilized properly. One alternative utilization is to process it into chitosan, which is then formulated into liquid fertilizer. This study aimed to determine the effect of chitosan concentration based on swimming crab shells on the chemical properties of liquid fertilizer. The study was conducted from February to March 2025. Chitosan and liquid fertilizer production was conducted at the Fishery Products Biochemistry Laboratory, Pangkep State Agricultural Polytechnic. The method used was an experimental laboratory with a Completely Randomized Design (CRD), with three treatments and three replications, with chitosan concentrations of 2%, 4%, and 6%. Data were analyzed using One-Way ANOVA and the W-Tukey HSD test. If the assumption of homogeneity of variance was not met, Welch ANOVA with the Games-Howell test was used to determine differences in effects between treatments. The results showed that the treatment had a significant effect (P<0.05) on most of the chemical properties of liquid fertilizer. The chemical properties of pH were (2.90 3.00 3.23), C-organic were (1.08% 2.72% 4.21%), macronutrients N + P2O5 + K2O were (0.76% 0.64% 1.19%). In conclusion, increasing the concentration of chitosan improves the chemical properties of liquid fertilizer, so that crab shell chitosan has the potential as a material for liquid organic fertilizer formulation.
AKTIVITAS ANTIBAKTERI TUMBUHAN DARAT DAN PESISIR DARI SULAWESI SELATAN TERHADAP PENYAKIT VIBRIO Basir, Buana; Kariyanti, Kariyanti; Isnansetyo, Alim
MARLIN Vol 4, No 1 (2023): (Februari) 2023
Publisher : Politeknik Kelautan dan Perikanan Pangandaran

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.15578/marlin.V4.I1.2023.49-56

Abstract

Salah satu alternatif penanganan penyakit bakteri Vibrio sp. adalah dengan penggunaan bahan bioaktif alami dari tumbuhan yang dapat menggantikan peran antibiotik Penelitian bertujuan untuk menganalisis aktivitas antibakteri tumbuhan darat dan pesisir dari Sulawesi Selatan terhadap bakteri Vibrio sp.. Penelitian dilaksanakan pada bulan April sampai dengan Agustus 2018, dengan pengambilan sampel uji di daerah Sulawesi Selatan. Penelitian dilakukan dengan metode percobaan dengan beberapa tahapan, yaitu persiapan sampel, maserasi sampel, partisi, dan uji aktivitas antibakteri. Sampel yang diuji sebanyak 30 sampel yang berasal dari 26 jenis tanaman dengan konsentrasi 500 µg/mL dan 1000 µg/mL. Jenis vibrio yang digunakan adalah V. parahaemolitycus, V. harveyii, dan V. alginolitycus. Analisis data dilakukan secara kualitatif berdasarkan zona hambat yang terbentuk. Berdasarkan hasil uji aktivitas antibakteri dari crude ekstrak ditemukan enam sampel yang memiliki aktivitas anti-vibrio. yaitu daun sukun, kulit buah sukun, kulit batang sukun. daun jara, daun avicennia, dan daun miana, dengan masing-masing nilai aktivitas sebesar 12 mm, 10 mm, 9 mm, 12,5 mm, 16 mm, dan 19 mm. Aktivitas antibakteri tertinggi dihasilkan oleh crude ekstrak daun miana pada fraksi etanol sebesar 23-24 mm.One alternative treatment for the bacterial disease is Vibrio sp. with natural bioactive ingredients from plants that can replace the role of antibiotics. The research aimed to analyze the antibacterial activity of miana leaves against Vibrio sp. The research was carried out from April to August 2018, by taking test samples in the South Sulawesi area. The study was conducted using an experimental method with several stages, sample preparation, sample maceration, partitioning, and antibacterial activity test. The samples tested were 30 samples from 26 types of plants with concentrations of 500 µg/mL and 1000 µg/mL. The types of vibrios used were V. parahaemolitycus, V. harveyii, and V. alginolitycus. Data analysis was carried out qualitatively based on the inhibition zones formed. Based on the results of the antibacterial activity test of the crude extract, it was found that six samples had anti-vibrio activity namely breadfruit leaves, breadfruit skin, breadfruit bark. Jara leaves, Avicennia leaves, and Miana leaves, with activity values of 12 mm, 10 mm, 9 mm, 12.5 mm, 16 mm, and 19 mm, respectively. Crude miana leaf extract had the highest antibacterial activity 23-24 mm ethanol production.