Article Per Year (5 Year)
p-Index From 2021 - 2026
2.908
P-Index
This Author published in this journals
All Journal Bioma : Berkala Ilmiah Biologi Jurnal Ilmu Lingkungan Biota: Jurnal Ilmiah Ilmu-Ilmu Hayati Biosfer: Jurnal Tadris Biologi Jurnal Biologi Tropis Jurnal Biogenerasi BIO-EDU: Jurnal Pendidikan Biologi Biofarm : Jurnal Ilmiah Pertanian BIOVALENTIA: Biological Research Journal Kenanga : Journal of Biological sciences and Applied Biology Journal Of Agriculture Sciences (Agrosci) Journal of Community Service (Abdisci)
Pratiwi, Dian Neli
Unknown Affiliation
Agriculture, Biological Sciences & Forestry Biochemistry, Genetics & Molecular Biology Education Environmental Science Immunology & microbiology Languange, Linguistic, Communication & Media Medicine & Pharmacology Social Sciences Other

Published : 20 Documents Claim Missing Document
Claim Missing Document
Check
Articles

Found 20 Documents
Search

 1   2 
AMPLIFIKASI GEN N-METHYLTRANSFERASE KOPI ROBUSTA DI PERKEBUNAN KOPI RAKYAT WIYONO LAMPUNG: AMPLIFIKASI GEN N-METHYLTRANSFERASE KOPI ROBUSTA DI PERKEBUNAN KOPI RAKYAT WIYONO LAMPUNG Lestari, Septi Wahyu; Rustiati, Elly Lestari; Priyambodo, Priyambodo; Wahyuningsih, Sri; Sandra, Shifa; Winarno, Winarno; Thesalonika, Natasya; Febriansyah, Muhammad; Ashari, Minanti Mayda; Andriyani, Yuliana; Afandi, Aril; Maharani, Annisa Lidya; Pratiwi, Dian Neli; Srihanto, Eko Agus; Suhada, Suhada; Permatasari, Nindy
Jurnal Biogenerasi Vol. 10 No. 2 (2025): Volume 10 no 2 periode februari - september 2025 ( continues)
Publisher : Universitas Cokroaminoto Palopo

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.30605/biogenerasi.v10i2.5544

Abstract

Kopi robusta (Coffea canephora) merupakan salah satu tanaman perkebunan yang umum ditanam di Indonesia karena memiliki nilai ekonomis tinggi. Kabupaten Pesawaran menjadi salah satu daerah di Lampung yang banyak mengusahatanikan kopi robusta dalam skala perkebunan rakyat. Isolasi dan amplifikasi gen menjadi langkah awal dalam analisis molekuler untuk mengetahui keragaman gen pada kopi robusta. Penelitian ini bertujuan untuk menganalisis hasil isolasi dan amplifikasi DNA daun kopi robusta dari perkebunan kopi rakyat Pesawaran, Lampung. Isolasi DNA dari 5 sampel daun kopi robusta telah dilakukan mengacu pada protokol kit isolasi DNA dari Geneaid Genomic DNA Mini Kit (Plant). Hasil ekstraksi kemudian diamplifikasi menggunakan primer N-methyltransferase dengan metode Polymerase Chain Reaction (PCR). Visualisasi hasil ekstraksi dan amplifikasi dilakukan menggunakan elektroforesis gel agarosa 1%. Visualisasi atas hasil ekstraksi telah menunjukkan diperolehnya ekstrak DNA yang baik. Visualisasi atas hasil amplifikasi menunjukkan telah diperolehnya fragmen DNA sepanjang 600bp.
Phylogenetic Construction of Stingless Bees in Bandar Lampung based on 16S rRNA Gene Priyambodo, Priyambodo; Rustiati, Elly Lestari; Pratiwi, Dian Neli; Susanto, Alvin Wiwiet; Srihanto, Eko Agus; Saswiyanti, Enny
Biosfer: Jurnal Tadris Biologi Vol 15 No 2 (2024): Biosfer: Jurnal Tadris Biologi
Publisher : UNIVERSITAS ISLAM NEGERI RADEN INTAN LAMPUNG

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.24042/biosfer.v15i2.24589

Abstract

Stingless bees are one of the honey-producing groups that are currently in increasing demand. The significant rise in honey demand necessitates research into the variety of stingless bee species that have the potential to produce high-quality honey in large quantities. This study aims to determine the genetic relationship of stingless bees from two colonies in Rajabasa, Bandar Lampung. Samples were collected from two different colonies in Rajabasa, Bandar Lampung. Molecular analysis was conducted, including DNA extraction, amplification, DNA sequencing, and phylogenetic analysis using the 16S rRNA gene. The study assessed homology values, genetic distance, and the phylogenetic map. The results indicated that the individual samples, GB1 and GB2, found in Bandar Lampung, were closely related to the species Heterotrigona itama. This was confirmed by the query value of both samples being 100%, with similarity values of 96.99% and 97.20%, respectively. The genetic distance analysis, visualized in the phylogenetic map, also shows that the two samples are close relatives of Heterotrigona itama.ABSTRAK: Lebah tanpa sengat menjadi salah satu kelompok penghasil madu yang saat ini makin banyak diminati masyarakat. Peningkatan kebutuhan madu yang signifikan, memerlukan penelitian tentang ragam spesies lebah tanpa sengat yang potensial dalam menghasilkan madu yang berkualitas prima dalam jumlah yang melimpah. Penelitian ini telah dilakukan dengan tujuan untuk mengetahui kedekatan genetik lebah tanpa sengat dari dua koloni di Rajabasa, Bandar Lampung. Sampel diambil dari dua koloni yang berbeda di Rajabasa, Bandar Lampung. Analisis molekuler telah dilakukan dengan tahapan ekstraksi DNA, amplifikasi DNA, sekuensing DNA, dan dilanjutkan dengan analisis filogenetik dengan menggunakan gen 16S rRNA. Analisis dilakukan untuk mengetahui nilai homologi, jarak genetik, dan peta filogenetik. Hasil menunjukkan bahwa individu sampel GB1 dan GB2 yang ditemukan di Bandar Lampung mempunyai kedekatan dengan spesies Heterotrigona itama. Hal ini ditunjukkan dengan nilai query keduanya sebesar 100% dan nilai similarity masing-masing adalah 96,99% dan 97,20%. Analisis jarak geneti yang divisualisasi dalam peta filogenetik juga menunjukkan bahwa kedua sampel yang diperoleh merupakan kerabat dekat dari Heterotrigona itama.
Species Confirmation for Robusta Coffee in Sedayu, Semaka, Tanggamus: Social Forestry Data Base Management Rustiati, Elly Lestari; Priyambodo, Priyambodo; Srihanto, Eko Agus; Pratiwi, Dian Neli; Susanto, Alvin Wiwiet; Febriansyah, Muhammad; Kusuma, Andriyani Wijaya; Saswiyanti, Enny
Jurnal Ilmu Lingkungan Vol 23, No 4 (2025): July 2025
Publisher : School of Postgraduate Studies, Diponegoro Univer

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.14710/jil.23.4.1149-1154

Abstract

Lampung, including the Register 31 Pematang Arahan area which is next to the Bukit Barisan Selatan National Park, is known for its high biodiversity, and its coffee production. Gapoktanhut Lestari Sejahtera, consisting of 13 Forest Farmer Groups (KTH), practiced community-based forest coffee under the KPH Kotaagung Utara, Tanggamus, Lampung. To support the development of biodiversity data base for KPH Kotaagung Utara, molecular-based species identification for robusta coffee has been carried out under BLU LPPM Unila grant year 2023 and in collaboration with the Lampung Disease Investigation Center. Robusta coffee leaf samples from six KTH areas (Bumi Mulyo, Sido Makmur 1, Sido Makmur 2, Sido Makmur 3, Mandiri Jaya, and Murah Rejeki) were collected for molecular species confirmation based on the N-methyl transferase genetic marker. Molecular analysis included DNA extraction, amplification, and electrophoresis conducted at the Lampung Disease Investigation Center. Sequencing was based on the Coffea canephora CAF2 genetic marker. Bioinformatics analysis was performed using the Basic Local Alignment Search Tool (BLAST) and Molecular Evolutionary Genetics Analysis (MEGA) software version 6. Identical sequence lengths of samples in 6 KTH are varied in base pair length and nitrogen base sequences, ranging from 163-530 bp, with 5 out of 6 KTH having a genetic distance of 0.000%, while 1 sample had a genetic distance of 0.006%. It indicates that robusta coffee samples from 6 KTH areas are genetically closely related. It is supported by its phylogenetic tree, the coffee species cultivated by Gapoktanhut Lestari Sejahtera is molecularly confirmed to be robusta coffee, Coffea canephora.
CITIZEN RESEARCH PRACTICE: LEARNING DATA COLLECTION ON SUMATRAN SLOW LORIS -ELECTRICAL GRID INTERACTION IN LABUHAN RATU VII, EAST LAMPUNG Rustiati, Elly Lestari; Priyambodo, Priyambodo; Sandora, Desta; Dwitama , Harry Putra; Subagyo, Agus; Pratiwi, Dian Neli; Bayu , Raden Fadli; Sunandar, Sunandar; Ariyanto, Slamet Puji; Supriyadi, Tatang
Jurnal Abdisci Vol 3 No 4 (2026): Vol 3 No 4 Tahun 2026
Publisher : Ann Publisher

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.62885/abdisci.v3i4.913

Abstract

Background. Supporting the need for the electricity network to carry out daily activities was done by PLN including Penyulang Kecak, the Sribawono Substation for Labuhan Ratu VII, Labuhan Ratu, East Lampung, a WKNP buffer village, the natural habitat of sumatran slow lorises and the electricity grid. Aims. To discussion/interview with village officials related to areas affected by interaction with sumatran slow lorises, from representatives of Labuhan Ratu VII was conducted Methods. Community service in the introduction and collection of community-based data related to the interaction of sumatran slow lorises with the electricity network was carried out by meetings, discussions and companion visits Conclusion. The interaction of sumatran slow loris wildlife and the electrical grid can result its losses both injuries and fatalities. Mitigation conflict tools has been set. Strengthening the local communities supports, companionship on citizen research, community-based data collection assistance was done in Labuhan Ratu VII, East Lampung. Knowledge on sumatran slow loris, electrical grid and conflict mitigation was engaged. Implementation. Data collection based on rapid assesment includes the research perception, document and data collection in the field was introduced and applied
Qualitative Analysis of Partial 16S rRNA Amplicon of Mitochondrial Gene of Stingless Bees in Pesawaran Priyambodo, Priyambodo; Rustiati, Elly Lestari; Pratiwi, Dian Neli; Susanto, Alvin Wiwiet; Imtitsal, Aulia; Fahrezi, Aditya; Febriansyah, Muhammad; Kusuma, Andriyani Wijaya; Srihanto, Eko Agus; Saswiyanti, Enny; Sidik, Mahfud; Sa'uddah, Lousanja D.; Lestari, Indah Ayu; Yani, Ani Andri; Ramadhan, Viki
Jurnal Biologi Tropis Vol. 23 No. 1 (2023): Special Issue
Publisher : Biology Education Study Program, Faculty of Teacher Training and Education, University of Mataram, Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.29303/jbt.v23i1.6470

Abstract

Pesawaran is one of the areas that has the potential to cultivate stingless bees as producers of honey and propolis. This study was aimed at qualitatively analysing partial amplicons of the 16S rRNA gene from individuals found at the research site. The research was performed from April to September 2023 in an explorative approach. Based on the exploration that has been done in Harapan Jaya Village, Way Ratai District, Pesawaran Regency, individual stingless bees were obtained from 5 different colonies. Individual samples of stingless bees were further extracted and amplified by commercial kits. Each individual from 5 different colonies has successfully obtained its DNA amplicons. Based on qualitative analysis using 1% agarose gel, the size of the partial gene ranged from 400bp to 500bp. This result is in accordance with the data contained in the genbank, where the 16S rRNA gene size is more than 500bp. Therefore, it has been concluded that the size of the partial amplicons of the 16S rRNA gene of individual stingless bees from the 5 colonies that were obtained is actually in range of the size of the 16S rRNA gene of stingless bees that have been recorded in the genbank.
Konstruksi Filogenetik Spesies Lebah Tanpa Sengat di Lampung Timur Berdasarkan Gen 16S rRNA Imtitsal, Aulia; Priyambodo; Rustiati, Elly Lestari; Pratiwi, Dian Neli; Susanto, Alvin Wiwiet; Ramadhan, Viki; Fahrezi, Aditya; Febriansyah, Muhammad; Kusuma, Andriyani Wijaya; Srihanto, Eko Agus; Saswiyanti, Enny
Biota : Jurnal Ilmiah Ilmu-Ilmu Hayati Vol 10, No 3 (2025): October 2025
Publisher : Universitas Atma Jaya Yogyakarta

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.24002/biota.v10i3.9506

Abstract

Keanekaragaman flora dan fauna Indonesia sangat beragam. Jenis fauna yang terkenal di Indonesia adalah lebah tanpa sengat yang tersebar di seluruh wilayah di Indonesia. Informasi keragaman lebah tanpa sengat dan hubungan kekerabatan masing-masing kelompok lebah akan memberikan informasi terkait data populasi. Analisis molekuler berguna sebagai ilmu dan alat untuk melakukan identifikasi spesies. Analisis molekuler keragaman lebah tanpa sengat dapat menggunakan DNA mitokondria (mtDNA) pada lebah. Salah satu gen pengkode DNA mitokondria adalah gen 16S rRNA. Penelitian ini bertujuan untuk menganalisis sekuen sampel lebah tanpa sengat secara molekuler dan menyusun peta kekerabatan sehingga dapat menjadi data genetik dan informasi keanekaragaman spesie lebah tanpa sengat di Kabupaten Lampung Timur. Metode yang digunakan adalah  isolasi dan amplifikasi gen DNA, elektroforesis dan visualisasi, sekuensing dan analisis hasil sekuensing  menggunakan Basic Local Alignment Search Tool (BLAST), dan  konstruksi pohon filogenetik menggunakan Molecular Evolution Genetic Analysis (MEGA11). Berdasarkan penelitian ini 3 sampel lebah tanpa sengat yang didapatkan dari eksplorasi di Kabupaten Lampung Timur terdeteksi sebagai spesies Heterotrigona itama dan memiliki kekerabatan yang dekat dengan spesies sundatrigona sp dan paling jauh dengan Paratrigona lineatifrons. Hal ini sesuai dengan hasil uji BLAST dan analisis data molekuler menggunakan software Mega 11.
ONLINE AND SEMI-ONLINE MENTORING METHODS: COMMUNITY EMPOWERMENT STRATEGIES DURING THE NEW NORMAL PANDEMIC Rustiati, Elly Lestari; Virnarenata, Elsa; Priyambodo, Priyambodo; Alimudin, Salih; Asmi, Dwi; Santoso, Edi; Yulianti, Yanti; Pratiwi, Dian Neli; Suroso, Erdi
Jurnal Abdisci Vol 1 No 5 (2024): Vol 1 No 5 Tahun 2024
Publisher : Ann Publisher

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.62885/abdisci.v1i5.207

Abstract

 Community stewardship can be done directly. Direct interaction is commonly applied, appreciated, and easy to communicate. During the COVID-19 pandemic, direct interaction and visits are almost impossible due to activity limitations and social distancing. Online and semi-online interaction can be applied by inviting community limited representatives combined with the online method. Initiation on online communication is introduced. This project was conducted in companionship and training in Braja Harjosari and Labuhan Ratu VII, East Lampung. Following the introduction of the online and semionline methods, local people can apply and share their knowledge of the new normal paradigm.
TRAINING ON EDU-AGROTOURISM FINANCIAL GOVERNANCE OF KWT BINA PERTANI, KAMPUNG LIMAN BENAWI, TRIMURJO, CENTRAL LAMPUNG Rahayu, Sri Retnaning; Rustiati, Elly Lestari; Pratiwi, Dian Neli; Septiana, Ana; Al Amin, Muhammadi Ridho; Kurniawan, Angga
Jurnal Abdisci Vol 1 No 8 (2024): Vol 1 No 8 Tahun 2024
Publisher : Ann Publisher

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.62885/abdisci.v1i8.326

Abstract

A training session on Edu-agrotourism financial governance was conducted for KWT Bina Pertani, a farmer women's group in Kampung Liman Benawi, Trimurjo, Central Lampung, on January 26, 2024. The primary objective of this fundamental accounting and finance training was to enhance the understanding of financial management and the skills of KWT Bina Pertani members and village civil servants. The curriculum encompassed an overview of financial statements, rudimentary bookkeeping, the compilation of financial statements, and strategies for crop pricing. The training objectives were achieved through coordination, agreement, training materials and equipment preparation, and activities and evaluations. The training was delivered face-to-face with direct practice, and the participants enthusiastically received it. The training session yielded positive outcomes, with successful material delivery and achievement of the activity objectives.
INITIATION OF ECONOMIC TRANSFORMATION AT BRAJA GEMILANG BASED ON PRA: EMPOWERMENT MODEL FOR SUSTAINABLE DEVELOPMENT Subagyo, Agus; Lestari Rustiati, Elly; Priyambodo; Sendora, Desta; Pratiwi, Dian Neli; Wahyono, Agus; Suwarno
Jurnal Abdisci Vol 3 No 4 (2026): Vol 3 No 4 Tahun 2026
Publisher : Ann Publisher

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.62885/abdisci.v3i4.955

Abstract

Braja Gemilang, Braja Selebah, East Lampung, has great natural and social resource potential, but has not been explored optimally. Participatory Rural Appraisal (PRA) was applied as the main approach to explore the potential local economy that is environmentally friendly, form business groups, and develop partnership networks. Activities conducted in July – August 2025 included participatory mapping, focus group discussions, comparative study visits, and partnership facilitation for three priority ventures: mocaf/tiwul, cassava-based flour production, organic fertilizer, and sumatran rhino food plant nursery. The initial output includes the formation of business groups (mocaf house), initial collaboration. The PRA-based mentoring model and approach showed support in both local welfare and conservation.
Keragaman Genetik Kopi Robusta Berdasarkan Karakter Fenotipe Batang di Perkebunan Kopi Rakyat Bogorejo, Lampung: Genetic Diversity of Robusta Coffee Based on Stem Phenotypic Characters in Bogorejo Coffee Plantation, Lampung Thesalonika, Natasya; Sandra, Shifa; Priyambodo, Priyambodo; Rustiati, Elly Lestari; Winarno, Winarno; Lestari, Septi Wahyu; Ashari, Minanti Mayda; Andriyani, Yuliana; Maharani, Annisa Lidya; Afandi, Aril; Febriansyah, Muhammad; Pratiwi, Dian Neli; Permatasari, Nindy; Suhada, Suhada
Biofarm : Jurnal Ilmiah Pertanian Vol. 21 No. 1 (2025): BIOFARM JURNAL ILMIAH PERTANIAN
Publisher : Universitas Pekalongan

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.31941/biofarm.v21i1.5937

Abstract

Pengembangan bibit unggul pada tanaman kopi robusta dapat dilakukan dengan pengamatan pada karakter batang. Hal ini penting mengingat kopui robusta menjadi salah satu penyumbang devisi dan sumber komoditas uatam di Indonesia, khususnya Lampung. Penelitian dilaksanakan untuk mengamati keragaman genetik tanaman kopi robusta pada Perkebunan Kopi Rakyat di Desa Bogorejo, Kecamatan Gedong Tataan, Kabupaten Pesawaran, Provinsi Lampung berdasarkan karakteristik fenotipe batang. Penelitian dilakukan dengan purposive sampling pada 30 individu. Pengamatan kualitatif dilakukan pada lima karakter batang, sedangkan pengukuran karakter kuantitatif dilakukan pada 11 karakter batang. Hasil menunjukkan bahwa terdapat 11 individu yang mempunyai sambungan batang bawah-batang atas, dengan seluruh individu tanaman kopi robusta mempunyai warna dan permukaan batang yang seragam, baik batang bawah dan batang atas. Di sisi lain, pengamatan terhadap karakter kuantitatif menunjukkan ragam yang lebih tinggi sehingga nampak adanya pengaruh genetik dan lingkungan terhadap karakter fenotipe batang tanaman kopi. Adapun rerata karakter kuantitatif individu pada populasi tersebut adalah tinggi tanaman total 124,29 cm, tinggi batang bawah 98,30 cm, lingkar batang bawah 15,05 cm, diameter batang bawah 47,25 cm, tinggi batang atas 70,88 cm, lingkar batang atas 14,78 cm, diameter batang atas 46,41 cm, jumlah cabang ortrotop pada batang utama 1,60 buah, jumlah jabang plagiotrop pada tabang utama 8 buah, tinggi cabang ortotrop pertama pada batang utama 39,43 cm, dan tinggi cabang plagiotrop pertama pada batang utama 114,30 cm.
Co-Authors Adelia, Ersa Imelda Afandi, Aril Agus Subagyo Al Amin, Muhammadi Ridho Alfandi, Aril Alimudin, Salih Angga Kurniawan Ariyanto, Slamet Puji Arum, Setiawan Ashari, Minanti Mayda Azzahra, Laila Salwa Bayu , Raden Fadli Dwi Asmi Dwitama , Harry Putra Edi Santoso Eko Agus Srihanto, Eko Agus Elly Lestari Rustiati Elly Lestari, Rustiati Erdi Suroso Fahrezi, Aditya Febriansyah, Muhammad Febriyani, Dwi Ayu Firwantoni, Firwantoni Gina Dania Pratami, Gina Dania Imtitsal, Aulia Indah Ayu Lestari, Indah Ayu Jani Master, Jani Krismuniarti, Elisabeth Devi Kusuma, Andriyani Wijaya Lestari, Septi Wahyu Maharani, Annisa Lidya Novianasari, Tika P. Haryanto, Sugeng Permatasari, Nindy Priyambodo Priyambodo Priyambodo Priyambodo Priyambodo Raden Mohamad Herdian Bhakti Ramadan, Viki Ramadhan, Viki Rustiaati, Elly Lestari Sa'uddah, Lousanja D. Samsul , Bakri Sandora, Desta Sandra, Shifa Saswiyanti, Enny Sendora, Desta Septiana, Ana Sidik, Mahfud Sri Retnaning Rahayu, Sri Retnaning Suhada Suhada, Suhada Sunandar Sunandar Supriyadi, Tatang Susanto, Alvin Wiwiet Suwarno Thesalonika, Natasya Virnarenata, Elsa Wahyono, Agus WINARNO Winarno Winarno Winda, Indriyanti Yani, Ani Andri Yanti Yulianti Yanti, Yulianti Yuliana Andriyani