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All Journal Jurnal Penelitian Sains Jurnal Riset Kesehatan Poltekkes Depkes Bandung INDONESIAN JOURNAL OF MEDICAL LABORATORY SCIENCE AND TECHNOLOGY JPP Jurnal Kesehatan Poltekkes Palembang Jurnal Sintesis: Penelitian Sains, Terapan dan Analisisnya Current Biomedicine Mukhtabar: Journal of Medical Laboratory Technology Jurnal Kesehatan Siliwangi Jurnal Pengabdian Masyarakat Kesehatan Indonesia Media Penelitian dan Pengembangan Kesehatan Jurnal Bioteknologi & Biosains Indonesia (JBBI) Jurnal Info Kesehatan Jurnal Bioteknologi & Biosains Indonesia
Merdekawati, Fusvita
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Agriculture, Biological Sciences & Forestry Biochemistry, Genetics & Molecular Biology Chemical Engineering, Chemistry & Bioengineering Chemistry Decision Sciences, Operations Research & Management Dentistry Education Environmental Science Health Professions Immunology & microbiology Materials Science & Nanotechnology Mathematics Medicine & Pharmacology Nursing Physics Public Health Social Sciences Veterinary

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Journal : Mukhtabar: Journal of Medical Laboratory Technology

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Optimization of Concentration and Staining Duration of Methyl Green in The Examination of Escherichia coli DNA Bands Using Agarose Gel Electrophoresis Adrian Prasetya, Rifky; Merdekawati, Fusvita; Iin Nur Indra, Asep; Djuminar, Ai
Mukhtabar Journal of Medical Laboratory Technology Vol 2 No 2 (2024): Mukhtabar: Journal of Medical Laboratory Technology (October 2024)
Publisher : LPPM STIKes Muhammadiyah Ciamis

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.52221/mjmlt.v2i2.696

Abstract

Background & Objective: Nowadays, many alternative dyes are used for staining DNA during electrophoresis, one of which is Methyl Green. Methyl Green has several advantages, including being cheaper than EtBr, having low toxicity, being non-carcinogenic, and as a cationic dye, Methyl Green is resistant to photobleaching. This study aims to determine the optimal concentration and staining duration of Methyl Green as a DNA dye for examining Escherichia coli DNA bands using agarose gel electrophoresis. Method: The research method used is experimental. Amplified Escherichia coli 16S rRNA gene DNA, sized 584 bp, which has undergone electrophoresis, was stained with Methyl Green dye at concentrations of 0.10%, 0.15%, 0.20%, 0.25%, 0.00015%, 0.00020%, 0.00025%, and 0.00030% with varying immersion times of 10, 15, 20, and 25 minutes. Result: The resulting DNA bands were analyzed or measured for surface area using ImageJ software. The mean value for each experimental group was calculated. The highest mean value was used as the basis for determining the most optimal condition. Conclusion: This study concludes that the optimal concentration and staining duration of Methyl Green, based on the highest mean value of 19,844,845, is 0.00030% Methyl Green with a staining duration of 25 minutes.
Assessment of Analytical Specificity in qPCR: SYBR Green and TaqMan Probe Methods for Isonazid-Resistant Mycobacterium tuberculosis Merdekawati, Fusvita; Suryawan, Muhammad Raihan; Nurhayati, Betty; Rinaldi, Sonny Faisal; Juliastuti, Aditya
Mukhtabar Journal of Medical Laboratory Technology Vol 3 No 1 (2025): Mukhtabar: Journal of Medical Laboratory Technology (April 2024)
Publisher : LPPM STIKes Muhammadiyah Ciamis

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.52221/mjmlt.v3i1.793

Abstract

Background & Objective: Mycobacterium tuberculosis, often referred to as M. tuberculosis, is an infectious pathogen that is responsible for causing tuberculosis (TB), a prevalent condition that is a leading cause of death globally. The spread of tuberculosis bacteria that are resistant to certain medicines, such as isoniazid, is currently on the rise. Therefore, molecular testing, such as quantitative polymerase chain reaction (qPCR), is needed to rapidly and reliably identify isoniazid-resistant tuberculosis germs. Method: The purpose of this study was to assess the analytical specificity of TaqMan Probe and SYBR Green qPCR techniques for the detection of isoniazid-resistant tuberculosis. This study was descriptive and quantitative. Analytical specificity was ascertained using MTB DNA with the S315G mutation spiked with E. coli DNA. A paired t-test was used to assess the primary data. Result: The results show that the analytical specificity values based on the significance of the paired t-test for the SYBR Green and TaqMan Probe methods were 0.398 and 0.790, respectively. Conclusion: Based on these results, it can be concluded that the analytical specificity of the TaqMan Probe qPCR method was 1.99 times greater than that of the SYBR Green method.
Co-Authors Abror, Yogi Khoirul Adrian Prasetya, Rifky Anhar, Citra Amaniah Dewi Nurhayati Djuminar, Ai Dzulhizza, Zaskia Puteri Endarwati, Dwi Veni Endrique Putri, Salmanda Ernawati Ernawati Febriyanti, Intan Hardiana, Acep Tantan Hasan, Zulfikar Ali Hayati, Eem Heri Setiyo Bekti I Gusti Agung Ayu Dharmawati Iin Nur Indra, Asep Iis Kurniati Indra , Asep Iin Nur Indra, Asep Iin Nur Irawan, Danni Juliastuti, Aditya Khairunnisa, Syirin Nisrina Khoirul Abror, Yogi Kurnaeni, Nani Lesiani, Bunga Rossa Maynar, Larasati Putri Nina Marliana, Nina Noviar, Ganjar nur amani putri, afifah Nur Habibah Nurhayati, Betty Nurhayati, Nuli Pratama, Resta Ratna Juwita Rahayu, Ira Gustira Rahmawati, Tia Rinaldi, Sonny Faisal Rinaldi, Sonny Feisal Rohayati rohayati rohayati Saputra, Fitrianingsih Shabrina, Almas Simangunsong, Kristina Solihat, M. Firman Sufa, Hafizah Ilmi Sulaeman, Rizal Pratama Sundara Mulia, Yuliansyah Suryawan, Muhammad Raihan Syafitri, Andita Izmi Tantan, Acep Trimulyani, Annisa Zahrani, Azka Hasbia Zanuba, Haifanisya Zahra Zuri Rismiarti