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All Journal Jurnal Teknologi Laboratorium Jurnal Penelitian Kesehatan Suara Forikes Health Notions THE JOURNAL OF MUHAMMADIYAH MEDICAL LABORATORY TECHNOLOGIST Window of Health : Jurnal Kesehatan Jurnal Ilmu dan Teknologi Kesehatan Jurnal Analis Medika Biosains (JAMBS) INDONESIAN JOURNAL OF MEDICAL LABORATORY SCIENCE AND TECHNOLOGY Poltekita : Jurnal Ilmu Kesehatan JPP Jurnal Kesehatan Poltekkes Palembang Medica Arteriana (Med-Art) Medicra (Journal of Medical Laboratory Science/Technology) CENDEKIA : Jurnal Ilmu Pengetahuan Devotion: Journal of Research and Community Service International Journal of Advanced Health Science and Technology HEALTHY: Jurnal Inovasi Riset Ilmu Kesehatan Analis Kesehatan Sains (e-Journal) Jurnal Rakat Sehat : Pengabdian Kepada Masyarakat Jurnal Pengabdian Kesehatan Beguai Jejama Strada Jurnal Ilmiah Kesehatan (SJIK) Folia Medica Indonesiana
Evy Diah Woelansari
Jurusan Teknologi Laboratorium Medis, Poltekkes Kemenkes Surabaya Surabaya
Biochemistry, Genetics & Molecular Biology Chemistry Computer Science & IT Dentistry Education Electrical & Electronics Engineering Health Professions Immunology & microbiology Medicine & Pharmacology Neuroscience Nursing Public Health Social Sciences Veterinary Other

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Validation of Rapid Antibody (IgM – IgG) Test Kit for SARS-CoV-2 Infection in Surabaya, Indonesia Museyaroh Museyaroh; Puspa Wardani; Aryati Aryati; Evy Diah Woelansari
JURNAL INDONESIA DARI ILMU LABORATORIUM MEDIS DAN TEKNOLOGI Vol 4 No 2 (2022): Enhanced knowledge of laboratory medicine's role in healthcare
Publisher : Universitas Nahdlatul Ulama Surabaya

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.33086/ijmlst.v4i2.3027

Abstract

At the beginning of the Corona Virus Disease 2019 (COVID-19) pandemic, rapid test examinations were widely used as a screening for Severe Acute Respiratory Syndrome Coronavirus-2 (SARS-CoV-2) infection. The purpose of this examination was to detect SARS-CoV-2 IgM/IgG antibodies in the patient's body. One of these tests uses the immunochromatographic method. This study aims to determine the validity of immunochromatography. The study was conducted from August to September 2020. The sample used in this study was 100 patients. The research was conducted at Husada Utama Hospital Surabaya, Indonesia. According to the study's findings, the Zybio brand reagent kit has an accuracy of 85%, a sensitivity of 82%, a specificity of 88%, a positive predictive value of 87%, and a negative predictive value of 83%. In the group of patients who experienced clinical symptoms, < 7 had a sensitivity of 50%, specificity of 88%, positive predictive value of 60%, negative predictive value of 83%, and accuracy of 77.94% while the group of patients experiencing clinical symptoms > 7 days, had a sensitivity value of 100 %, specificity of 88%, positive predictive value of 84%, negative predictive value of 100%, and accuracy of 92.68%. Based on these results, the conclusion is that the Zybio brand reagent kit has a relatively high sensitivity, specificity, positive predictive value, negative value, and sample accuracy. In the group with clinical sensitivity < 7 days, the positive predictive value and accuracy are lower than the sample group with clinical symptoms > 7 days but have the same specificity.
KORELASI HASIL UJI WIDAL DENGAN RAPID TEST IgG DAN IgM PADA PASIEN SUSPEK DEMAM TIFOID Fazira Azmi Haq; Evy Diah Woelansari; Christ Kartika Rahayuningsih; Wisnu Istanto
HEALTHY : Jurnal Inovasi Riset Ilmu Kesehatan Vol. 5 No. 4 (2026)
Publisher : Pusat Pengembangan Pendidikan dan Penelitian Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.51878/healthy.v5i4.14121

Abstract

Typhoid fever is a systemic infectious disease caused by Salmonella enterica serovar Typhi and requires laboratory examinations to support diagnosis. The Widal test and IgG/IgM rapid test are serological examinations that are still widely used in healthcare facilities, but they have different test characteristics. This study aimed to analyze the correlation between Widal test results and IgG and IgM rapid test results in patients with suspected typhoid fever at Dr. H. Moh. Anwar Regional General Hospital, Sumenep. This study employed an analytical observational design with a cross-sectional approach and purposive sampling technique. The study was conducted from February 2 to 28, 2026, involving 35 patients with suspected typhoid fever. The examinations included the slide agglutination Widal test and immunochromatographic IgG/IgM rapid test. Data were analyzed using Spearman's correlation test with a significance level of p < 0.05. The results showed a significant correlation between the Widal test and the IgG rapid test, with a correlation coefficient of r = 0.667 and p < 0.001, indicating a strong positive correlation. A significant correlation was also found between the Widal test and the IgM rapid test, with r = 0.861 and p < 0.001, indicating a very strong positive correlation. In conclusion, there was a significant correlation between Widal test results and both IgG and IgM rapid test results in patients with suspected typhoid fever. These findings indicate an association between the results of the two serological examinations; however, their interpretation should consider the patient's clinical condition and the stage of disease progression. ABSTRAK Demam tifoid merupakan penyakit infeksi sistemik yang disebabkan oleh Salmonella enterica serovar Typhi dan memerlukan pemeriksaan penunjang untuk membantu diagnosis. Uji Widal dan rapid test IgG/IgM merupakan pemeriksaan serologis yang masih digunakan di fasilitas pelayanan kesehatan, tetapi memiliki karakteristik pemeriksaan yang berbeda. Penelitian ini bertujuan menganalisis korelasi hasil uji Widal dengan rapid test IgG dan IgM pada pasien suspek demam tifoid di RSUD Dr. H. Moh. Anwar Sumenep. Penelitian menggunakan desain observasional analitik dengan pendekatan cross-sectional dan teknik purposive sampling. Penelitian dilaksanakan pada 2–28 Februari 2026 dengan jumlah sampel sebanyak 35 pasien suspek demam tifoid. Pemeriksaan dilakukan menggunakan uji Widal metode slide aglutinasi dan rapid test IgG/IgM metode imunokromatografi. Data dianalisis menggunakan uji korelasi Spearman dengan tingkat kemaknaan p < 0,05. Hasil penelitian menunjukkan terdapat korelasi signifikan antara hasil uji Widal dengan rapid test IgG dengan koefisien korelasi r = 0,667 dan p < 0,001 yang menunjukkan hubungan kuat. Korelasi antara hasil uji Widal dengan rapid test IgM juga signifikan dengan r = 0,861 dan p < 0,001 yang menunjukkan hubungan sangat kuat. Dengan demikian, terdapat korelasi signifikan antara hasil uji Widal dengan rapid test IgG dan IgM pada pasien suspek demam tifoid. Hasil tersebut menunjukkan adanya keterkaitan antara hasil kedua pemeriksaan serologis, tetapi interpretasinya tetap perlu mempertimbangkan kondisi klinis dan fase perjalanan penyakit.
GAMBARAN PEMERIKSAAN RHEUMATOID FACTOR PADA LANSIA DI WILAYAH KERJA PUSKESMAS PUCANG SEWU SURABAYA Widya Kartika Sari; Evy Diah Woelansari; Museyaroh Museyaroh; Dwi Krihariyani
CENDEKIA: Jurnal Ilmu Pengetahuan Vol. 6 No. 4 (2026)
Publisher : Pusat Pengembangan Pendidikan dan Penelitian Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.51878/cendekia.v6i4.14049

Abstract

Rheumatoid Factor (RF) is an autoantibody commonly used as a supporting laboratory test for the identification of Rheumatoid Arthritis (RA). Rheumatoid Arthritis is an autoimmune disease characterized by chronic inflammation of the joints and may occur among older adults. This study aimed to describe the results of Rheumatoid Factor examination among elderly individuals in the working area of Pucang Sewu Public Health Center, Surabaya. This study employed a descriptive design involving 34 elderly respondents selected using purposive sampling based on predetermined inclusion and exclusion criteria. Rheumatoid Factor examination was performed using the latex agglutination method. Samples with reactive results were subsequently examined using a semi-quantitative method to determine the Rheumatoid Factor titer. Data were analyzed descriptively and presented in frequency distribution tables based on Rheumatoid Factor results, sex, and age group. The results showed that 31 respondents (91.2%) had negative Rheumatoid Factor results, while 3 respondents (8.8%) had positive results. All respondents with positive results had a Rheumatoid Factor titer of 16 IU/mL. Based on sex, all positive results were found among male respondents. Based on age group, positive results were found among respondents aged 60–69 years and 80–89 years. It can be concluded that most elderly individuals in the working area of Pucang Sewu Public Health Center, Surabaya, had negative Rheumatoid Factor results, while a small proportion had positive results with low titers. ABSTRAK Rheumatoid Factor (RF) merupakan salah satu autoantibodi yang digunakan sebagai pemeriksaan penunjang dalam identifikasi Rheumatoid Arthritis (RA). Rheumatoid Arthritis merupakan penyakit autoimun yang ditandai dengan peradangan kronis pada sendi dan dapat ditemukan pada kelompok lanjut usia. Penelitian ini bertujuan untuk mengetahui gambaran hasil pemeriksaan Rheumatoid Factor pada lansia di wilayah kerja Puskesmas Pucang Sewu Surabaya. Penelitian ini menggunakan desain deskriptif dengan melibatkan 34 responden lansia yang dipilih menggunakan teknik purposive sampling berdasarkan kriteria inklusi dan eksklusi. Pemeriksaan Rheumatoid Factor dilakukan menggunakan metode aglutinasi lateks. Sampel dengan hasil reaktif selanjutnya diperiksa secara semikuantitatif untuk menentukan titer Rheumatoid Factor. Data dianalisis secara deskriptif dan disajikan dalam bentuk tabel distribusi frekuensi berdasarkan hasil pemeriksaan Rheumatoid Factor, jenis kelamin, dan kelompok usia. Hasil penelitian menunjukkan bahwa 31 responden (91,2%) memiliki hasil Rheumatoid Factor negatif, sedangkan 3 responden (8,8%) menunjukkan hasil positif. Seluruh responden dengan hasil positif memiliki titer Rheumatoid Factor sebesar 16 IU/mL. Berdasarkan jenis kelamin, seluruh hasil positif ditemukan pada responden laki-laki. Berdasarkan kelompok usia, hasil positif ditemukan pada kelompok usia 60–69 tahun dan 80–89 tahun. Dapat disimpulkan bahwa sebagian besar lansia di wilayah kerja Puskesmas Pucang Sewu Surabaya memiliki hasil Rheumatoid Factor negatif, sedangkan sebagian kecil menunjukkan hasil positif dengan titer rendah.
Co-Authors , Suhariyadi ., Pestariati A.A. Ketut Agung Cahyawan W Abror, Yogi Khoirul Adelia Gita Prasasti Alifiah, Salsabiil Rochman Aliya Ayu Rizqiyah Anggraini, Anita Dwi Anita Dwi Anggraini Aprilyadi, Nadi Arifin, Syamsul Diah Aryati Aryati Cheppy Juwita Ayuningrum Christ Kartika Rahayuningsih Christiyaningsih, Juliana Diah Titik Mutiarawati Edy Haryanto Eka Safitri, Devayanti Endah Astuti, Sri Sulami Endarini, Lully Hanni Entuy Kurniawan Kurniawan Fadia Rakhmalia Fazira Azmi Haq Fortuna Aliza Dewi Handayati, Anik Hariyanto Hariyanto Istanto, Wisnu istiqomah istiqomah Istiqomah Istiqomah Juliana Christyaningsih Kartika Rahayuningsih, Christ Krihariyani, Dwi Kumahartati, Intan Liliek Soetjiatie Luthfi Rusyadi Mamik Mamik Mamik Mamik Minarti Minarti Minarti Minarti Mohd Nazil Salleh Museyaroh, Museyaroh Mutiarawati, Diah Titik Nabilah, Musholli Himmatun Niswata, Navila Arfianti Novikasari, Caturizkyana Prasasti, Adelia Gita Purnomowati, Anik Purwati Purwati Puspa Wardani Rahayuningsih, Christ. Kartika Rahma Yuliana, Putri Ramadhany, Rieke Dwi Retno Sasongkowati Retno Sasongkowati Retno Sasongkowati Rieke Dwi Ramadhany Riyanto, Digdo Rizqiyah, Aliya Ayu Salsabiil Rochman Alifiah Sasongkowati, Retno Sri Utami Suhariyadi Suhariyadi Suhariyadi Suhariyadi Suliati Suliati Suliati Suliati Syafira, Nurah Widiastutik, Dyah Widya Kartika Sari Wisnu Istanto Wisnu Istanto