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All Journal Jurnal Teknologi Laboratorium Jurnal Penelitian Kesehatan Suara Forikes Health Notions THE JOURNAL OF MUHAMMADIYAH MEDICAL LABORATORY TECHNOLOGIST Window of Health : Jurnal Kesehatan Jurnal Ilmu dan Teknologi Kesehatan Jurnal Analis Medika Biosains (JAMBS) INDONESIAN JOURNAL OF MEDICAL LABORATORY SCIENCE AND TECHNOLOGY Poltekita : Jurnal Ilmu Kesehatan JPP Jurnal Kesehatan Poltekkes Palembang Jaringan Laboratorium Medis Medica Arteriana (Med-Art) Medicra (Journal of Medical Laboratory Science/Technology) CENDEKIA : Jurnal Ilmu Pengetahuan Devotion: Journal of Research and Community Service International Journal of Advanced Health Science and Technology HEALTHY: Jurnal Inovasi Riset Ilmu Kesehatan Analis Kesehatan Sains (e-Journal) Jurnal Rakat Sehat : Pengabdian Kepada Masyarakat Jurnal Pengabdian Kesehatan Beguai Jejama Strada Jurnal Ilmiah Kesehatan (SJIK) Folia Medica Indonesiana
Evy Diah Woelansari
Jurusan Teknologi Laboratorium Medis, Poltekkes Kemenkes Surabaya Surabaya
Biochemistry, Genetics & Molecular Biology Chemistry Computer Science & IT Dentistry Education Electrical & Electronics Engineering Health Professions Immunology & microbiology Medicine & Pharmacology Neuroscience Nursing Public Health Social Sciences Veterinary Other

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STUDI SURVEI : GAMBARAN PENYAKIT MENULAR PADA WANITA DI KABUPATEN MAGETAN Riyanto, Digdo; Woelansari, Evy Diah; Nabilah, Musholli Himmatun
HEALTHY : Jurnal Inovasi Riset Ilmu Kesehatan Vol. 4 No. 3 (2025)
Publisher : Pusat Pengembangan Pendidikan dan Penelitian Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.51878/healthy.v4i3.6793

Abstract

ABSTRACT Sexually Transmitted Infections (STIs) such as HIV, Hepatitis B, and Syphilis are serious public health problems, especially among high-risk groups like female sex workers. The Pasar Sayur Magetan area has a high potential for STI transmission due to nighttime entertainment activities and the presence of dimly lit establishments. This study aims to identify the presence of STIs among women in Magetan as a basis for planning prevention and education programs by the government and health authorities. This research uses an analytical cross-sectional survey design with venous blood samples collected in EDTA anticoagulant tubes from female sex workers at Pasar Sayur Magetan. Data were collected through Rapid Diagnostic Tests (RDT) using immunochromatographic methods to detect HIV, Hepatitis B, and Syphilis, along with questionnaires to identify risk factors and behaviors influencing STI transmission. The study found cases of HIV and Syphilis among female sex workers in Pasar Sayur Magetan. The main risk factors include unsafe sexual behavior, inconsistent condom use, frequent partner changes, and limited education and knowledge. Although all respondents practiced good genital hygiene, this did not fully prevent STIs transmitted through blood and bodily fluids. This survey reveals the prevalence of STIs and the main risk factors in Pasar Sayur Magetan. These findings serve as a foundation for the government and health services to design prevention, education, and early detection programs to reduce cases and increase awareness of safe sexual behavior. ABSTRAK Infeksi Menular Seksual (IMS) seperti HIV, Hepatitis B, dan Sifilis menjadi masalah kesehatan serius, terutama bagi kelompok berisiko tinggi seperti wanita pekerja seks. Kawasan Pasar Sayur Magetan berpotensi tinggi dalam penyebaran IMS karena aktivitas hiburan malam dan keberadaan warung remang-remang. Penelitian ini bertujuan untuk mengetahui adanya penyakit penyakit menular seksual pada wanita di daerah Magetan, agar dapat digunakan sebagai dasar dalam perencanaan program pencegahan dan edukasi oleh pemerintah dan dinas Kesehatan. Penelitian ini menggunakan desain survei analitik cross-sectional dengan menggunakan sampel darah vena antikoagulan EDTA pada wanita pekerja seks di Pasar Sayur Magetan. Data dikumpulkan dari hasil pemeriksaan Rapid Diagnostic Test (RDT) metode imunokromatografi untuk mendeteksi HIV, Hepatitis B, dan Sifilis, serta kuesioner untuk mengidentifikasi faktor risiko dan perilaku yang mempengaruhi penularan IMS. Penelitian ini menemukan adanya kasus HIV dan Sifilis di kalangan wanita pekerja seks di Pasar Sayur Magetan dengan faktor risiko utama berupa perilaku seksual tidak aman dan ketidakkonsistenan penggunaan kondom serta keterbatasan pendidikan dan pengetahuan. Kesimpulannya, meskipun praktik genital higiene baik, risiko penularan IMS tetap terjadi. Survei ini mengungkap tingkat penyebaran PMS di Pasar Sayur Magetan serta faktor risiko utama. Temuan ini menjadi dasar bagi pemerintah dan dinas kesehatan dalam merancang program pencegahan, edukasi, dan deteksi dini guna mengurangi kasus serta meningkatkan kesadaran akan perilaku seksual yang aman.
Comparison of Platelet Index in Type 2 Diabetes Mellitus Patients With and Without Diabetic Ulcers Rahma Yuliana, Putri; Suhariyadi, Suhariyadi; Woelansari, Evy Diah
Devotion : Journal of Research and Community Service Vol. 6 No. 11 (2025): Devotion: Journal of Community Research
Publisher : Green Publisher Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.59188/devotion.v6i11.25573

Abstract

Hyperglycaemia is caused by poor insulin production and insulin resistance, which is the beginning of type 2 diabetes mellitus. The blockage of blood vessels caused by this disease is due to the increase in blood sugar levels, which is the cause of diabetic ulcers. As a result, patients are often unaware of their injuries. This research aims to identify differences in platelet indices (MPV, PDW and PCT) in people with type 2 diabetes mellitus who have diabetic ulcers and those who do not. This research uses observational analysis methods and a cross-sectional analysis approach. The independent variable in this study was type 2 diabetes mellitus patients with and without diabetic ulcers, while the dependent variable in this study was the platelet value, consisting of MPV in type 2 diabetes mellitus patients with and without diabetic ulcers of 7,716 fL and 7,837 fL. The mean PCT in type 2 diabetes mellitus patients with and without diabetic ulcers was 0.225% and 0.245%, respectively. The mean PDW in type 2 diabetes mellitus patients with and without diabetic ulcers was 18.825% and 14.237%, respectively. The results of data analysis using unpaired t-test showed a p-value of 0.00 for PDW and 0.002 for MPV, so it was concluded that there were differences in MPV and PDW in type 2 diabetes mellitus patients who had diabetic ulcers and those who did not.
Lymphocyte Count and SARS-CoV-2 Antibody Level in Healthy Donors' Blood at an Indonesian Blood Transfusion Center Prasasti, Adelia Gita; Woelansari, Evy Diah; , Suhariyadi; Anggraini, Anita Dwi
Folia Medica Indonesiana Vol. 59, No. 4
Publisher : Folia Medica Indonesiana

Show Abstract | Download Original | Original Source | Check in Google Scholar

Abstract

Highlights: 1. It is essential to conduct research on SARS-CoV-2 for the purpose of acquiring further understanding, especially concerning the production of antibodies examined using antibody titer blood tests. 2. Although the relationship between the examined variables is not significant, this study offers valuable information on blood test results after the COVID-19 vaccination, which can serve as scientific evidence for further research. Abstract Severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) is a virus that infects the respiratory system by attacking the mucous and epithelial cells. This infection commonly leads to an increase in lymphocyte count as an immune response to invading pathogens. Moreover, antibodies bind and inactivate foreign substances to destroy pathogens and inhibit their replication. These mechanisms prompt the objective of this study, which was to define the relationship between lymphocyte count and SARS-CoV-2 antibody level. This analytical observational study used a cross-sectional approach with quantitative analysis methods and purposive sampling. Healthy donors who had received coronavirus disease (COVID-19) vaccines provided the samples for this study. A total of 30 blood samples were collected from the Blood Transfusion Center of the Indonesian Red Cross Surabaya Area. This study was conducted in May 2022 at two distinct locations. The examination of lymphocytes was carried out using the flow cytometry method in the Hematology Laboratory, Department of Medical Laboratory Technology, Politeknik Kesehatan Kemenkes Surabaya, Surabaya, Indonesia. In addition, the antibody titer test using the enzyme-linked immunosorbent assay (ELISA) method was performed in the Immunoserology Laboratory of the Surabaya Health Laboratory Center, Surabaya, Indonesia. The analysis revealed an average lymphocyte concentration of 2.2633 x 103/µl and an average antibody level of 0.2197 according to the optical density (OD) ratio. The data analysis was performed using Spearman's rank correlation statistical test (p<0.005), and the results indicated a lack of significance with p=0.262. In conclusion, there is no relationship between total lymphocyte count and SARS-CoV-2 antibody level.
Perbandingan Pemeriksaan Skrining Antibodi (Indirect Coombs Test) dengan Direct Coombs Test pada Pasien dengan Riwayat Transfusi Darah Berulang Arta, Shela Yuni; Suhariyadi, Suhariyadi; Woelansari, Evy Diah; Rahayuningsih, Christ Kartika
Jaringan Laboratorium Medis Vol. 7 No. 2 (2025): November 2025
Publisher : Poltekkes Kemenkes Semarang

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.31983/jlm.v7i2.13643

Abstract

Repeated blood transfusions can increase risk of alloimmunization and autoimunization, which causes formation alloantibodies and autoantibodies. The presence of these antibodies can make cross-matching more difficult and increase the possibility of hemolytic transfusion risk. Antibody screening tests (Indirect Coombs Test) and Direct Coombs Test play a role in detecting the presence of alloantibodies and autoantibodies in patient with a history of repeated transfusion. The aim of the study was to determine the diferences in results between antibody screening (Indirect Coombs Test) and Direct Coombs Test in patients receiving repeated blood transfusions. This study used a quasi-experimental design with a cross-sectional approach. The subjects were patients with a history of blood transfusions more than 5 times at RSUD R.T. Notopuro Sidoarjo totalling 60 samples. The data obtained were then analyzed statistically to see if there were any significant differences between the two types of examinations. The results obtained showed a significant difference between the results of the antibody screening test (ICT) and the Direct Coombs Test (DCT) (p-value 0.000), that more patients with repeated blood transfusions had positive DCT results (75%). Some patients showed positive results on one or both types of tests, indicating the presence of alloantibodies or autoantibodies. ICT and DCT tests provide complementary information in detecting antibodies in patients with repeated transfusions. The use of both is important in improving the safety of blood transfusions and minimizing the risk of hemolytic reactions.
Co-Authors , Suhariyadi ., Pestariati A.A. Ketut Agung Cahyawan W Abror, Yogi Khoirul Adelia Gita Prasasti Alifiah, Salsabiil Rochman Aliya Ayu Rizqiyah Anggraini, Anita Dwi Anita Dwi Anggraini Aprilyadi, Nadi Arifin, Syamsul Diah Arta, Shela Yuni Aryati Aryati Ayu Puspitasari, Ayu Cheppy Juwita Ayuningrum Christ Kartika Rahayuningsih Christiyaningsih, Juliana Diah Titik Mutiarawati Edy Haryanto Eka Safitri, Devayanti Endarini, Lully Hanni Entuy Kurniawan Kurniawan Evi Yunita Nugrahini Fadia Rakhmalia Fortuna Aliza Dewi Ginarsih, Yuni Handayati, Anik Hariyanto Hariyanto Hermiyanti, Pratiwi Ira Puspita Sari Ira Rahayu Tiyar Istanto, Wisnu istiqomah istiqomah Istiqomah Istiqomah Juliana Christyaningsih Kasiati Kiaonarni O.W Krihariyani, Dwi Kumahartati, Intan Lembunai Tat Alberta Liliek Soetjiatie Lully Hani Endarini Luthfi Rusyadi Mamik Mamik Mamik Mamik Minarti Minarti Minarti Minarti Mohd Nazil Salleh Mujayanto Museyaroh, Museyaroh Mutiarawati, Diah Titik Nabilah, Musholli Himmatun Niswata, Navila Arfianti Novikasari, Caturizkyana Nur Hatijah Nurwening TW P, Teresia Retna Pertiwi, Resty Cahya Prasasti, Adelia Gita Purnomowati, Anik Purwati Purwati Puspa Wardani Rahayu Sumaningsih Rahayuningsih, Christ Kartika Rahayuningsih, Christ. Kartika Rahma Yuliana, Putri Ramadhany, Rieke Dwi Retno Sasongkowati Retno Sasongkowati Retno Sasongkowati Rieke Dwi Ramadhany Riyanto, Digdo Rizqiyah, Aliya Ayu Salsabiil Rochman Alifiah Sasongkowati, Retno Sri Sulami Endah Astuti Sri Utami Suhariyadi Suhariyadi Suhariyadi Suhariyadi Suhariyadi Suhariyadi Suhariyadi Suhariyadi Suhariyadi Suhariyadi Suliati Suliati Suliati Suliati Suliati Sumasto, Hery Syafira, Nurah Teta Puji Rahayu Widiastutik, Dyah Wisnu Istanto Yohanes Kambaru W Zerlinda, Nur