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Hubungan Kekerabatan Kepiting Rajungan (Portunus sp.) Berdasarkan DNA Barcoding di Perairan Percut Sei Tuan Kabupaten Deli Serdang Zuhra, Arini; Manalu, Kartika; Idami, Zahratul
Jurnal Biogenerasi Vol. 10 No. 1 (2024): Volume 10 Nomor 1, Agustus 2024 - Februari 2025
Publisher : Universitas Cokroaminoto Palopo

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.30605/biogenerasi.v10i1.4834

Abstract

Rajungan crab (Portunus sp.) is one type of crab that has a fairly high commodity value, this species has a fairly wide distribution covering the Indo-Pacific Ocean and also offshore, this crab has an important role in ecological stability. Portunus sp. has a more attractive appearance compared to other crabs. The purpose of this study is to look at the (dominant) crab species found in January (west wind season) in the waters of Percut Sei Tuan based on the genes CO1, and phylogenetics (kinship relationship) of Portunus sp. in the waters of Percut with Portunus sp. in NCBI or Genebank. The method used in this study is DNA isolation on specimens using the Favorgen kit locus of the CO1 gene in the genome of the Portunus sp. amplified by PCR technique with LCO1940 (forward) and HCO2198 (reverse) primers. The results of the research that have been carried out have found 3 species from the genus Portunus, namely P. sanguinolentus, P. trituberculatus and P. pelagicus. Of the three species found in the Percut Sei Tuan Waters, P. trituberculatus and P. pelagicus have a closer kinship relationship compared to P. sanguinolentus, based on the percentage of AT content and GC content, the AT content is higher at 61.03% which means that the three species have primitive properties. The genetic distance between P. sanguinolentus and P. trituberculatus is closer (19.32) compared to P. pelagicus. Based on the phylogenetic tree reconstruction, P. trituberculatus and P. sanguinolentus are closer to P. pelagicus with the Neighbor-Joining method and the Kimura-2-Parameter calculation model. Thus, it can be concluded that DNA barcoding using the CO1 gene locus can be used as a method in identifying Portunus sp. molecularly and kinship relationships with other species.
ANALISIS MOLEKULER KERANG (Anadara sp.) MENGGUNAKAN MARKER GEN CYTOCHROME OXYDASE SUBUNIT 1 (CO1) DI PERAIRAN PERCUT SEI TUAN Dwi Pratiwi, Anggrainy; Idami, Zahratul; Manalu, Kartika
Jurnal Biogenerasi Vol. 10 No. 1 (2024): Volume 10 Nomor 1, Agustus 2024 - Februari 2025
Publisher : Universitas Cokroaminoto Palopo

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.30605/biogenerasi.v10i1.4835

Abstract

Anadara sp. clams are very popular clams among the community. Anadara sp. also usually live in waters with muddy and sandy surfaces. DNA barcoding is a barcode sequence to identify unknown species. CO1 is a molecular marker commonly used in molecular research on animals. The purpose of this study was to determine the species of Anadara sp. clams found in Percut Sei Tuan Waters using gene markers, the diversity of Anadara sp. clams using CO1 Gene Markers, and the kinship of Anadara sp. clams found in Percut Sei Tuan Waters. This study has 4 stages, namely DNA isolation using the Favorgen kit, PCR amplification, electrophoresis and sequencing. The DNA sequencing results were analyzed using the MEGA 11 application. The results showed that of the three samples that had been analyzed using the CO1 gene marker obtained using BLAST and NCBI, namely the Anadara granosa, Anadara antiquata, and Anadara gubernaculum samples. With the calculation results of higher AT content (28.9%) and lower GC content (20.12%) which states that the species is more primitive. In the pairwise distance matrix, the samples of Anadara antiquata (Percut Sei Tuan) and Anadara granosa (Percut Sei Tuan) which are in the same clade with different sub clades have a genetic distance ranging from 111.45 to 213.77. The phylogenetic tree also shows that the three samples have a close kinship relationship.
Analisis Komponen Literasi Sains pada Buku Teks Biologi SMA/Ma Kelas X Marsyah, Shafira Hana; Manalu, Kartika; Rambe, Riris Nurkholidah
Jurnal Simki Pedagogia Vol 6 No 2 (2023): Volume 6 Nomor 2 Tahun 2023
Publisher : Universitas Nusantara PGRI Kediri

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.29407/jsp.v6i2.222

Abstract

This study aims to analyze the category of scientific literacy in class X biology textbooks at MAS Nurul Iman Mahato. The research used in this research uses a qualitative approach with content analysis. Content analysis is commonly used in analyzing the validity of the contents of a book to draw conclusions from the contents of books and documents using certain procedures. This research was conducted at MAS Nurul Iman located in Mahato Bandar Selamat Village, North Tambusai District, Rokan Hulu Regency, Riau Province with biology textbooks for class X in MAS Nurul Iman Mahato. Data collection used using interview and observation techniques. Analyzing the data obtained, the steps taken to obtain analysis data from the data that have been obtained by 1) Summing the occurrence of scientific indicators from each component of the scientific literacy category, 2) Calculating the percentage of occurrence of scientific literacy indicators in the books being analyzed 3) Calculating the reliability of the data 4) Draw Conclusions. From the results of the research discussed earlier, it can be concluded that there are several percentages of the appearance of the scientific literacy category in class X biology textbooks at MAS Nurul Iman Mahato. Viewed from the aspect of science as the body of knowledge, the presentation of scientific literacy in class X biology textbooks in MAS Nurul Iman Mahato of 46.68%, seen from the aspect of science as a way of knowledge that the presentation of scientific literacy in class X biology textbooks at MAS Nurul Iman Mahato is 19.55%, viewed from the aspect of science as a way of thinking that the presentation of scientific literacy in class X biology textbooks at MAS Nurul Iman Mahato by 13.30%, seen from the aspect of science as an aspect of science interaction with technology and social that the presentation of scientific literacy in class X biology textbooks at MAS Nurul Iman Mahato is 13.99 %.
Analisis Buku Teks Pembelajaran Biologi SMA Kelas XI Kurikulum 2013 Berdasarkan Literasi Ilmiah Di Kecamatan Barumun Kabupaten Padang Lawas Pulungan, Erlinda Marito; Manalu, Kartika; Jayanti, Ummi Nur Afinni Dwi
Spizaetus: Jurnal Biologi dan Pendidikan Biologi Vol 4 No 1 (2023): Spizaetus: Jurnal Biologi dan Pendidikan Biologi
Publisher : Program Studi Pendidikan Biologi, FKIP, Universitas Nusa Nipa

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.55241/spibio.v4i1.118

Abstract

Penelitian ini bertujuan untuk mendeskripsikan tingkat literasi ilmiah buku teks pelajaran Biologi SMA kelas XI berdasarkan tema literasi ilmiah dan secara khusus penelitian ini tekanan pada dimensi literasi ilmiah.Penelitian ini menggunakan pendekatan kualitatif dengan metode (content analysis). setiap bab pada buku teks biologi. Analisis data dilakukan dengan metode dokumentasi Hasil penelitian pada kedua buku menunjukkan bahwa literasi sains sebagai jalan investigasi menjadi dimensi yang paling dominan muncul pada buku I sebesar 62,5% sedangkan pada buku II sebesar 60%. Dimensi sains sebagai batang tubuh proporsinya lebih banyak pada buku I yaitu sebesar 60%. Sedangkan pada buku II 50%.Sedikit sekali bagian yang menekan dimensi sains sebagai jalan berpikir dengan proporsi rata-rata pada buku I sebesar 40%, sedangkan pada buku II sebesar 32.5% . Dimensi sains dan interaksinya dengan teknologi dan masyarakat muncul paling sedikit dan kurang ditekankan pada kedua buku dengan jumlah rata-rata sebesar 25%. Dari hasil tersebut dapat disimpulkan bahwa tingakat literasi ilmiah buku teks pelajaran biologi SMA kelas XI yang digunakan di Kecamatan Barumun cukup baik sesuai dengan penilaian penilaian