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All Journal Jurnal Biologi dan Pembelajarannya (JBP) JURNAL PENGABDIAN KEPADA MASYARAKAT Jurnal Biologi Tropis JURNAL PENDIDIKAN TAMBUSAI Jurnal Mangifera Edu Jurnal Review Pendidikan dan Pengajaran (JRPP) Biopendix: Jurnal Biologi, Pendidikan dan Terapan Jurnal Pembelajaran dan Biologi Nukleus Best Journal (Biology Education, Sains and Technology) Jurnal Biogenerasi SOSIOEDUKASI : JURNAL ILMIAH ILMU PENDIDIKAN DAN SOSIAL Spizaetus: Jurnal Biologi dan Pendidikan Biologi JURNAL BIOSHELL Bioscientist : Jurnal Ilmiah Biologi Jurnal Simki Pedagogia TEACHING : Jurnal Inovasi Keguruan dan Ilmu Pendidikan Bioed : Jurnal Pendidikan Biologi Jurnal Pijar MIPA Pedagogika: Jurnal Ilmu-Ilmu Kependidikan Jurnal Biologi
Manalu, Kartika
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Agriculture, Biological Sciences & Forestry Arts Humanities Biochemistry, Genetics & Molecular Biology Chemistry Economics, Econometrics & Finance Education Environmental Science Health Professions Immunology & microbiology Languange, Linguistic, Communication & Media Library & Information Science Mathematics Physics Public Health Social Sciences Other

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IDENTIFIKASI KERAGAMAN MORFOLOGI DAUN TANAMAN UBI KAYU (Manihot escolenta Cantz) BERDASARKAN PENANDA RAPD (RANDOM AMPLIFIED POLYMORPHIC DNA) Hazmi, Hafiza; Idami, Zahratul; Manalu, Kartika
Jurnal Biogenerasi Vol. 11 No. 1 (2026): Volume 11, no 1, 2026, (Januari - Maret 2026)
Publisher : Universitas Cokroaminoto Palopo

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.30605/87gabb18

Abstract

Cassava Plants (Manihot esculenta Crantz), traditionally cultivated and offering many benefits as food ingredients, animal feed, and industrial raw materials. Cassava is also known to be rich in carbohydrates, soluble dietary fiber, and beneficial micronutrients for the body. Morphological and genetic diversity in cassava plants is crucial for the development of superior and improved varieties. This study aims to identify the morphological diversity of cassava leaf plants (Manihot esculenta Crantz) and analyze dendrogram relationships using RAPD (Random Amplified Polymorphic DNA) markers. The research was conducted in Serdang Bedagai Regency and the laboratory of the State Islamic University of North Sumatra, Medan. The methods used included sample collection, DNA isolation, PCR amplification, electrophoresis, and data analysis. The results showed variations in leaf morphology as observed in the number of leaflets, color, and shape of leaves in cassava varieties Genderuwo, Roti, Keriting, and Jari. Morphologically, the Genderuwo and Roti cassava leaves were the most similar. The dendrogram analysis using RAPD primers OPA 2, OPA 3, OPA 5, and OPA 7 showed a close relationship between Genderuwo and Roti varieties (coefficient 0.85), while the Jari variety had the most distant relationship (coefficient 0.60). This research can serve as a reference in cassava plant breeding to produce superior varieties  
ANALISIS FINGERPRINT DNA GENOTIPE BELIMBING DENGAN GENUS Averrhoa MENGGUNAKAN PRIMER RANDOM AMPLIFIED POLYMORPHIC Hikmah, Nur; Idami, Zahratul; Manalu, Kartika
Jurnal Biogenerasi Vol. 11 No. 1 (2026): Volume 11, no 1, 2026, (Januari - Maret 2026)
Publisher : Universitas Cokroaminoto Palopo

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.30605/5d69v436

Abstract

Fingerprinting is a technique in DNA technology that can be used to see individual diversity and can also differentiate individuals from each other even though they are very closely related. This study aims to see the results of DNA fingerprinting of starfruit genotypes with the genus Averrhoa using Random Amplified Polymorphic DNA (RAPD) primers and to see the polymorphism value of each Random Amplified Polymorphic DNA (RAPD) primer. The research method used is Fingerprint DNA with Random Amplified Polymorphic DNA (RAPD) primers, namely OPA-2, OPA-3, OPA-5, OPA-7, OPD-11 and OPD-13. The results of the research show that the fingerprint results can be seen clearly. using electrophoresis. The best results were the primers OPA 2, OPA3 with 6 band patterns, followed by the second highest number of OPA5, OPD 11 with 5 band patterns, and the third highest number of OPA7, OPD 13 with 4 DNA fingerprint band patterns, the results of the kinship relationship between each Averrhoa have The polymorphic value shows that sweet starfruit (Averrhoa carambola L.) has a relationship of around 0.62% with starfruit. wuluh ( Averrhoa bilimbi L.). The Demak starfruit (Averrhoa carambola Cultivar Demak) has a relationship of around 0.71% with the Sembiring starfruit (Averrhoa carambola).  
ANALISIS KERAGAMANGENETIK DNA UBI JALAR (Ipomea batatas L.)DENGAN MARKA RANDOM AMPLIFIED POLYMORPHIC DNA (RAPD)   Wulan Dari, Tri; Idami, Zahratul; Manalu, Kartika
Jurnal Biogenerasi Vol. 11 No. 1 (2026): Volume 11, no 1, 2026, (Januari - Maret 2026)
Publisher : Universitas Cokroaminoto Palopo

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.30605/2dg8y347

Abstract

Sweet potato (Ipomea batatas L.) is a national food security supported by one of the potential plant resources. After rice, corn, and cassava, sweet potato (Ipomea batatas L.) contains the fourth highest carbohydrate. Sweet potato is a good source of thiamine (vitamin B1), riboflavin, vitamin A, vitamin B, and vitamin C. The purpose of this study was to determine the genetics of the analysis results presented in sweet potato (Ipomea batatas L.) using dendograms and DNA banding patterns in sweet potatoes based on RAPD markers. The research method used is an experimental method to collect data by conducting direct experiments, isolating DNA, and conducting PCR using the RAPD technique; Six RAPD primers were used: OPA 2, OPA 3, OPA5, OPA7, OPD 11, and OPD 13. The study resulted in genetic diversity seen from the DNA banding pattern that forms polymorphism. RAPD produces bands measuring 100-900 bp. The genetic diversity of 4 types of Ipomea batatas L. plants analyzed using the NTSYS pc-2.02i application on the kinship relationship displayed with a dendogram shows the same diversity, namely orange sweet potatoes with yellow sweet potatoes, which have the highest level of similarity because they join at a coefficient value of around 0.91. While in purple sweet potatoes joining the group (yellow sweet potatoes and orange sweet potatoes) at a coefficient value of 0.86 indicates that purple sweet potatoes are slightly more different but still in the group. Then in white sweet potatoes have a connection coefficient value of around 0.81 which indicates that this sample is the most different compared to the others)
Co-Authors Adisty, Yuda Agusna, Sri Anas, Nirwana Andini, Pinkhan Aliffia Sekar Arbaiyah, Seri Aulya, Milda Ayundari, Sella Bintang, Safliah Rahmayani Br Hombing, Dinda Anisah Dwi Pratiwi, Anggrainy Fatimah Azzahra Fermani, Nuritia Fitri Amalia Ginting, Monika Habibunnisa, Habibunnisa Hasibuan, Ira Aqrabi Br Hasra, Barratun Nisa Hazmi, Hafiza Herni, Zulfiana Holila, Nur Hutasuhut, Melfa Aisyah Jayanti, Ummi Jayanti, Ummi Nur Afinni Dwi Julianty, Dwita Khadijah, Nur Khairuna Khairuna Kinanti, Aulia Andin Kirana, Fira Maha, Destri Br Marsyah, Shafira Hana Najah, Nurun Nasution, Rizki Amelia Nst, Rizki Amelia Nur Hikmah Pasaribu, Nurul Alda Pulungan, Erlinda Marito Putri Lubis, Febrianti Putri, Amelisa Rafika Annisa, Rafika Rahmadina, Rahmadina Rambe, Riris Nurkholidah Raselia, Citra Rasyidah Rasyidah Raudhatul Jannah Rohani Rohani Sartika, Dea Simarmata, Faried Raja Sinaga, Sabariah Siregar, Abdul Hasym Mazhurin Siregar, Khoirul Bahria Siti Fatimah Sucahyo, Ahmad Imam Tazkiah, Citra Utami, Mutiara Alya Widya Ulfa, Syarifah Wulan Dari, Tri Zahratul Idami Zelila, Umma Selma Zuhra, Arini