Article Per Year (5 Year)
p-Index From 2021 - 2026
3.874
P-Index
This Author published in this journals
All Journal EKSAKTA: Journal of Sciences and Data Analysis Jurnal Florea Buletin Anatomi dan Fisiologi (Bulletin of Anatomy and Physiology) Biomolecular and Health Science Journal Best Journal (Biology Education, Sains and Technology) Indonesia Berdaya Indonesian Community Journal Jurnal Pengabdian Masyarakat Bangsa ABDI DAYA: Jurnal Pengabdian dan Pemberdayaan Masyarakat SWAGATI: Journal of Community Service Edubiotik : Jurnal Pendidikan, Biologi dan Terapan Genbinesia Journal of Biology
Rofiatun Solekha
Universitas Muhammadiyah Lamongan
Agriculture, Biological Sciences & Forestry Arts Humanities Biochemistry, Genetics & Molecular Biology Chemical Engineering, Chemistry & Bioengineering Chemistry Civil Engineering, Building, Construction & Architecture Computer Science & IT Decision Sciences, Operations Research & Management Earth & Planetary Sciences Economics, Econometrics & Finance Education Energy Engineering Environmental Science Health Professions Immunology & microbiology Industrial & Manufacturing Engineering Law, Crime, Criminology & Criminal Justice Materials Science & Nanotechnology Mathematics Medicine & Pharmacology Neuroscience Nursing Physics Public Health Social Sciences Veterinary Other

Published : 23 Documents Claim Missing Document
Claim Missing Document
Check
Articles

Found 23 Documents
Search

 1   2   3 
Comparative Expression Analysis of Geraniol Biosynthesis genes in Differents Accessions of Cymbopogon nardus Solekha, Rofiatun; Sabilla Khoirotunnisa; Siti Lathifatul Mahmudah; Puput Virnanda Yuniar; R. Achmad Djazuli
Florea : Jurnal Biologi dan Pembelajarannya Vol. 13 No. 1 (2026)
Publisher : UNIVERSITAS PGRI MADIUN

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.25273/florea.v13i1.24357

Abstract

Citronella (Cymbopogon nardus) is rich in absorbing flavonoids, and its precious geraniol is superior, none can be compared. Despite having a number of well-known cultivars of citronella in Indonesia with unique bioactive potential, the expression profiles of genes and geraniol profile content across these plants remain unknown. Here we report on the relative expression of crucial biosynthetic and regulatory genes involved in geraniol production, analyzed in relation to β-carotene levels and geraniol content measured from five different Indonesian citronella cultivars. Gene expression was quantified using real-time quantitative PCR (RT-qPCR), targeting nine genes: CnDXS, CnIDI, CnGPPS, CnGES, CnLIS, CnUGT, CnMYB, CnGEDH, and CnGDP. Geraniol content was determined according to High Performance Liquid Chromatography (HPLC). Citrona 1 Agribun and Citrona 2 Agribun showed higher expression of the upstream biosynthetic genes (CnGES, CnGPPS) and the regulatory gene CnMYB for which showed also good linearity with highest geraniol 24.71 mg/L and 26.35 mg/L, respectively. These results demonstrate a positive expression levels and geraniol content, highlighting the transcriptional complexity in citronella geraniol biosynthesis. The conclussion, suggest possible molecular candidates for enhancing contents in biofortification scenarios (e.g., selective breeding or genome editing) for essential oil quality.
Kajian Etnobotani Tumbuhan Obat oleh Masyarakat di Kecamatan Modo Kabupaten Lamongan Ramadhan, Muhamad Syahrur; Solekha, Rofiatun; Tamam, Muhammad Badrut
BEST Journal (Biology Education, Sains and Technology) Vol 8, No 2 (2025): September 2025
Publisher : Program Studi Pendidikan Biologi

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.30743/best.v8i2.13389

Abstract

Indonesia merupakan negara megabiodiversitas dengan kekayaan hayati dan budaya yang luar biasa, terdiri dari sekitar 300 etnis yang masing-masing memiliki warisan budaya, termasuk pengetahuan tradisional tentang tumbuhan obat etnobotani mempelajari hubungan manusia dan tumbuhan, menjadi solusi pelestarian pengetahuan tradisional dengan mendokumentasikan pemanfaatan tumbuhan obat dengan metode observasi dan wawancara. Hasil penelitian menunjukkan jenis tumbuhan obat yang digunakan adalah asam jawa, beluntas, jahe jambu biji, kencur, kunyit, lengkuas, mengkudu, salam, sambiroto, sirih, tapak liman, yodium. Pemanfaatkan sebagai pelangsing, pegal linu, menghangatkan badan, nafsu makan, meredakan batuk, mual, diare, sembelit, meredakan asam lambung, menurunkan tekanan darah, kolesterol, darah tinggi, pegal pegal, membersihkan keputihan, meningkatkan kekebalan tubuh, menyembuhkan luka. Cara pengolahan tumbuhan obat adalah dengan cara rebus, dioles dan dikeringkan penelitian ini menunjukkan bahwa masyarakat Kecamatan Modo memiliki pengetahuan tradisional yang cukup kaya mengenai tumbuhan obat dan cara pemanfaatannya, yang berpotensi untuk dikembangkan dalam pelestarian kearifan lokal dan pengobatan alternatif. Berdasarkan hasil analisis Presentase Nilai Penggunaan PPV bagian tumbuhan yang paling banyak dimanfaatkan adalah batang (rimpang) kunyit (Curcuma longa L.) dengan nilai 46,30%. Dari data yang dihasilkan Tingkat Kepercayaan (Fidelity Level) masyarakat Kecamatan Modo adalah 31,6%.
Bioactif and Bioactivity Test of Moringa (Moringa sp.), Starfruit Wuluh (Averrhoa bilimbi), and Keres (Muntingia calabura) Latifah, Indah Ttriwahyuni; Solekha, Rofiatun; Haibaturrahma, Nur Fatimah Azzahra; Wulandari, Ayu Dewi; Ayuni, Rieke Dwi; Nisa, Salasun; Alfanani, Syafiq; Ramadhan, Muhammad Syahrur; Khakim, Arsanul
BEST Journal (Biology Education, Sains and Technology) Vol 8, No 1 (2025): Juni 2025
Publisher : Program Studi Pendidikan Biologi

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.30743/best.v8i1.10729

Abstract

This study evaluated the bioactivity of Moringa plant extracts (Moringa oleifera), Star Fruit Wuluh (Averrhoa bilimbi), and Keres (Muntingia calabura) on antioxidant and antibacterial activities. Extraction was carried out using the maceration method with a 96% ethanol solvent. The antioxidant test was carried out using the DPPH method (2,2-diphenyl-1- picrylhydrazyl), while the antibacterial test was carried out on Staphylococcus aureus bacteria using the well diffusion method. The results showed that Moringa extract had a strong linear relationship between concentration and antioxidant activity, while Star Fruit and Keres extracts showed low antioxidant activity with IC50 values above 100 ppm, indicating weak activity. In the antibacterial test, Star Fruit extract produced a significant inhibition zone, although it was classified as a weak response. These findings suggest that all three plants have potential as sources of bioactive compounds, although optimal efficacy requires optimization of extraction and concentration methods. Further research is needed to identify the main active compounds and explore their mechanisms of action.
Co-Authors Abdul Majid Aini, Nur Sofiatul Aisyah Hadi Ramadani Aisyah, Neelam Aji Naufal Syafiq Alfanani, Syafiq Aluisius Hery Pratono Angella Ananda Saputra Angella Ananda Syaputra Ayuni, Rieke Dwi A’in, Nur Khurotul Badriatul Musyarofah Badriatul Musyarofah Badriyatul Musyarofah Bagus Dwi Jauhari Bambang Budiarto, Bambang Chandra Tri uliana Sari Dimas Arya Nugraha Dini Ermavitalini Djumadi, Firman Rosjadi Djuwari Djuwari Dwi Dianita Irawan Dwi Zulianti Elliv Hidayatul Lailiyah Fadhilah, Mei Sasti Febianti Dwi Hapsari Febrianti, Rohmatul Ula Fika Nur Fitriana Putri Bukhori Finaty Ahsanah, Finaty Haibaturrahma, Nur Fatimah Azzahra Halma, emil Milhatul Maflahah Hariadi, Sugeng Hery Purnobasuki Humairah, Humairah Husna, Etika Ziadana Al Idfi Setyaningrum, Idfi Karin Alifia Rachmadani Karin Alifia Rachmadani Karin Alifia Rachmadani Khakim, Arsanul Khoirin Nisa Laila Ainur Rohmah Lailatus Fitri Latifah, Indah Ttriwahyuni Lilis Maghfuroh Mahbubillah, M. Ainul Mahbubilllah, M Ainul Mintarti Ariani Mufti Ari Bianto Muhibanah, Fina Naskhi, Rifqi Ahmad Sandi Ni'matuzahroh Ni'matuzahroh Nisa, Salasun Nynda Ayu Nadira Savitri Nynda Ayu Nadira Savitri Oktavian, Ahnaf Rafli Pangestika, Alvi Widya Prasetiyo, Agung Yudha Prastiyanto, Muhammad Evy Puput Virnanda Yuniar R. Achmad Djazuli Rahayu , Anisa Esti Rahmahana, Ratna Syifa’a Rahmawati, Isabela Selvi Rajamin, Irza Meingindra Putri Ramadhan, Muhamad Syahrur Ramadhan, Muhammad Syahrur Reny Rosalina Rusandini, Khaela Vici Sabilla Khoirotunnisa Safitri, Putri Farida Salasun Nisah Salsabila, Hana Salwa, Aulia Alfiyatus Setiawati, Etika Setiyowati, Putri Ayu Ika Silvi Wasi’atul Af'idah Siti Lathifatul Mahmudah Sri Bintang Sahara Mahaputra Kusumanegara Sundari, Made Siti Tamam, Muhammad Badrut Trisiya, Oktavia Wulandari, Ayu Dewi Yaumi, Sri Yuanita Rachmawati