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Rasmi, Dewa Ayu Citra
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Agriculture, Biological Sciences & Forestry Biochemistry, Genetics & Molecular Biology Chemical Engineering, Chemistry & Bioengineering Chemistry Economics, Econometrics & Finance Education Immunology & microbiology Library & Information Science Materials Science & Nanotechnology Physics Public Health Social Sciences Other

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Isolation and Identification of Endophytic Bacteria Producing IAA and Phosphate Solubilizers from Mangrove Roots in the Bagek Kembar Sekotong Area and In Vitro Testing on the Germination of Vigna radiata L. Havizah, Ilma Nur; Zulkifli, Lalu; Rasmi, Dewa Ayu Citra; Sedijani, Prapti
Jurnal Biologi Tropis Vol. 25 No. 4b (2025): Special Issue
Publisher : Biology Education Study Program, Faculty of Teacher Training and Education, University of Mataram, Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.29303/jbt.v25i4b.10069

Abstract

Mangroves are a potential source of plant growth-promoting bacteria (PGPB) due to their unique habitat. Exploring potential endophytes as biofertilizers to reduce dependence on chemical fertilizers. This study aims to determine the effect of IAA-producing endophytic bacteria and phosphate solubilizers from mangrove roots on the germination of mung beans (Vigna radiata L.). The bacteria were isolated from mangrove roots in the Bagek Kembar Mangrove Ecosystem Area, Sekotong, West Lombok. The study began with the isolation of endophytic bacteria, followed by characterization, including morphological identification, cell morphology (via Gram staining), and physiological properties (biochemical testing). The potential of endophytic bacteria to produce IAA was tested qualitatively (using colorimetry) and quantitatively (using a spectrophotometer at a wavelength of 530 nm). Additionally, the ability of endophytic bacteria to solubilize phosphate was tested both qualitatively (using a screening method) and quantitatively (using a spectrophotometer at a wavelength of 880 nm). To test the effect of endophytic bacteria on soybean germination, green soybean seeds were grown on modified Murphy medium for 5 days, with the observed parameters being plant height, root length, fresh weight, and dry weight. The results of the green soybean germination observations were analyzed using One-Way ANOVA. Based on the research results, 11 endophytic bacterial isolates were found to be capable of producing IAA in the range of 16.88 to 30.28 ppm. Meanwhile, in terms of phosphate solubility, based on the screening results, 6 out of 11 isolates were able to solubilize phosphate with an average dissolved phosphate concentration of 17.63 ppm on the 4th day of incubation. The results of the analysis of the effect of IAA-producing bacteria and phosphate solubilizers on the germination of green beans showed a significant effect on plant height, root length, fresh weight, and dry weight of green bean plants.
Isolation and Identification of IAA-Producing and Phosphate-Solubilizing Rhizobacteria from Sesuvium portulacastrum and In Vitro Growth-Promotion Effect on Vigna radiata L. Yuliana, Baiq Fadilla; Zulkifli, Lalu; Rasmi, Dewa Ayu Citra; Sedijani, Prapti
Jurnal Biologi Tropis Vol. 25 No. 4a (2025): Special Issue
Publisher : Biology Education Study Program, Faculty of Teacher Training and Education, University of Mataram, Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.29303/jbt.v25i4a.10070

Abstract

Sesuvium portulacastrum is a coastal plant resistant to high salt content and drought. Exploration of rhizosphere bacteria is necessary to obtain potential isolates that can be developed as biofertilizers in the future. This study aims to isolate and identify rhizosphere bacteria from Sesuvium portulacastrum that are able to produce Indole Acetic Acid (IAA) and dissolve phosphate, and to test their effects in vitro on the germination of Vigna radiata L. Rhizosphere bacteria from the Bagek Kembar Mangrove Ecosystem Area, Sekotong, West Lombok Regency. A total of 14 bacterial isolates were successfully obtained and characterized based on morphological, physiological, and biochemical properties. All isolates produced IAA qualitatively and quantitatively (19.85–33.71 ppm) with isolate KT1 having the highest production (33.71 ppm). Qualitative phosphate solubilization test showed that 12 isolates were able to form a clear zone, while quantitatively it ranged from 7.86 to 16.18 ppm with isolate K8 having the highest value (16.18 ppm). Selected isolates KT1 (the highest IAA producer), K8 (the highest phosphate solubilizer), and K5 (both with high abilities) were tested for their effect on mung bean germination. Inoculation had a significant effect on plant height, root length, and dry weight (p<0.05). These results indicate that S. portulacastrum rhizosphere bacteria have the potential to be an environmentally friendly biofertilizer for plants in high-salinity soils.
Endophytic Bacteria of Mangrove Roots from the West Coast of Lombok Island with Phosphate-Solubilizing and IAA-Producing Abilities as Biofertilizer Candidates Zulkifli, Lalu; Sakaroni, Rubiyatna; Rasmi, Dewa Ayu Citra; Wirajagat, Gde Cahyadi
Jurnal Biologi Tropis Vol. 25 No. 4b (2025): Special Issue
Publisher : Biology Education Study Program, Faculty of Teacher Training and Education, University of Mataram, Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.29303/jbt.v25i4b.11175

Abstract

Mangrove ecosystems are unique habitats with extreme environmental conditions that select for adaptive endophytic microorganisms, making them a potential source of plant growth-promoting bacteria (PGPB). Exploring the potential of endophytes as biofertilizers to reduce dependence on chemical fertilizers is crucial in the context of sustainable agriculture. This study aims to characterize the potential of mangrove root endophytic bacteria from the coast of Lombok as biofertilizer agents through the evaluation of Indole-3-Acetic Acid (IAA) hormone production and phosphate solubilization ability. Endophytic bacteria were isolated from roots, evaluated for IAA production using the Salkowski method, while phosphate solubilization ability was evaluated qualitatively on Pikovskaya agar and quantitatively in liquid culture using molybdenum blue spectrophotometry. Potential isolates were characterized morphologically to determine their bacterial genus. The results showed that all isolates produced IAA, with AV1 (51.52 ppm) and AV4 (42.86 ppm) categorized as high producers. Phosphate solubilization ability varied significantly, with AV1 showing the strongest activity (index 4.41), followed by M1 (1.93), while some isolates showed low to no activity. Quantitative tests showed a peak in dissolved phosphate on day 4, with M2 (18.20 ppm) and M1 (16.31 ppm) being the most efficient phosphate solubilizers. Phenotypic characterization identified all promising isolates (AV1, AV4, M1) as Gram-positive, rod-shaped bacteria belonging to the genus Bacillus sp. based on Bergey’s Manual of Systematic Bacteriology. Overall, AV1, AV4, and M1 were identified as the most promising candidates for further development as biofertilizers.
Japanese Papaya (Cnidoscolus aconitifolius): An Integrated Biological System with Mechanism-Based Utilization Sedijani, Prapti; Mahrus, Mahrus; Rasmi, Dewa Ayu Citra; Kusmiyati, Kusmiyati; Khaerudin, Khaerudin
Jurnal Biologi Tropis Vol. 25 No. 4a (2025): Special Issue
Publisher : Biology Education Study Program, Faculty of Teacher Training and Education, University of Mataram, Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.29303/jbt.v25i4a.11234

Abstract

Cnidoscolus aconitifolius (Japanese papaya or chaya) is a tropical plant from the Euphorbiaceae family known for its high nutritional value and diverse biological activities. Several studies have reported antioxidant, antimicrobial, and other biological properties, suggesting the involvement of complex molecular systems. This article presents a conceptual literature review using a systems biology approach to understand Japanese papaya as an integrated biological system in which nutritional components, secondary metabolites, and enzymatic activities interact. By positioning hydrolytic enzyme systems as the central mechanism, this review discusses the potential utilization of Japanese papaya as a biological resource and as a basis for the development of functional derivative products, including biomolecular hydrolysates. Possible applications in mechanism-based bioconversion processes are also briefly addressed.
Co-Authors A Wahab Jufri Ahmad Raksun, Ahmad Arfinal, Arfinal Arianti, Septia Fitri Aulia Havizah, Ilma Nur Heru Setiawan Hidayati, Siti Nur Isnaini I Gde Mertha I Wayan Merta Ilhamdi, M Liwa Jamaluddin Khaerudin Khaerudin Khairuddin Kusmiyati Kusmiyati Kusmiyati Kusuma, Anindita Suliya Hangesti Mandra LALU ZULKIFLI Lestari, Tri Ayu Mahrus Mahrus Mahrus, Mahrus Nathnia, Elga Amelinda Ni Luh Putu Pebri Artayani . Nurikhsanti, Mikiyal PRAPTI SEDIJANI, PRAPTI Sakaroni, Rubiyatna Saputri, Andini Sari, Neti Kumala Sativa, Denianto Yoga Sedijani , Prapti Sembiring, Rinawati Sukarso, Aa Sukarso, AA. Surhayani, Evi Utami, Ni Wayan Anggun Diah Wirajagat, Gde Cahyadi Yuliana, Baiq Fadilla Zanaria