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All Journal HAYATI Journal of Biosciences Jurnal Ilmu Pertanian Indonesia Jurnal Ilmu Dasar Biology, Medicine, & Natural Product Chemistry Jurnal Sumberdaya Hayati Makara Journal of Science Molekul: Jurnal Ilmiah Kimia
Prastya, Muhammad Eka
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Agriculture, Biological Sciences & Forestry Biochemistry, Genetics & Molecular Biology Chemistry Control & Systems Engineering Earth & Planetary Sciences Immunology & microbiology Materials Science & Nanotechnology Mathematics Medicine & Pharmacology Public Health

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Antioxidant and Cytotoxic Properties of Extract from Soil Bacteria Isolated from Muna Island, Southeast Sulawesi Priyanto, Jepri Agung; Hening, Egiyanti Nur Widhia; Permatasari, Vera; Prastya, Muhammad Eka; Hasidu, La Ode Abdul Fajar; Primahana, Gian
Jurnal ILMU DASAR Vol 25 No 1 (2024)
Publisher : Fakultas Matematika dan Ilmu Pengetahuan Alam Universitas Jember

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.19184/jid.v25i1.39244

Abstract

Soil bacteria could be the promising source of secondary metabolites with diverse pharmacological activities. Bacteria coded as P1 and M7 were isolated from soil obtained from Muna Island, Southeast Sulawesi. This research aimed to evaluate the antioxidant activity, cytotoxic property, and identify the chemical profile of the extract derived from soil bacteria P1 and M7. Based on 2,2'-azino-bis (3-ethylbenzothiazoline-6-sulfonic acid (ABTS) and 2,2-diphenyl-1-picrylhydrazyl (DPPH) assay, extract derived from P1 isolate was moderately active against ABTS free radical with IC50 of 126.22±10.82 µg/mL, and less active on DPPH (IC50: 721.95±16.59 µg/mL), while M7 extract was less active towards DPPH and ABTS. Interestingly, these extracts in the concentration of 100 µg/mL were cytotoxic to breast cancer cell (MCF-7), as tested by 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyl-2H-tetrazolium bromide (MTT) assay. MCF-7 viability was decreased up to 69.83±1.46 % and 61.49±0.76 % after treated with P1 and M7 extract, respectively. The extracts were also induced membrane disruption and apoptosis of MCF-7 cells. The extracts contained phenol (9.52±0.22 and 10.41±0.09 mg EAG/g extract, for P1 and M7, respectively) and flavonoids (9.16±0.16 and 9.29±0.24 mg EQ/g extract, for P1 and M7, respectively). According to Gas Chromatography Mass Spectrometry (GC-MS) analysis, Pyrrolo[1,2-a] pyrazine-1,4-dione, hexahydro-3-(2-methylpropyl) that previously well known as antioxidant and cytotoxic compounds, were found in the M7 and P1 extract. This compound may play a vital role in the radical scavenging and cytotoxic properties of P1 and M7 extracts. The results of this study indicate that P1 and M7 extracts could be the potential source of antioxidant and cytotoxic compounds and need to be further developed for pharmaceutical use.
Uji Aktivitas Antibakteri dan Antioksidan dari Metabolit Ekstraseluler Bakteri Endofit Tumbuhan Mahang (Macaranga bancana): Antibacterial and Antioxidant Activity Test of Extracellular Metabolites of Endophytic Bacteria of Mahang Plants (Macaranga bancana) Chang, Helen; Pujiyanto, Sri; Prastya, Muhammad Eka; Primahana, Gian; Yuswan, Apriza; Retnowati, Dwi; Permatasari, Vera
Jurnal Sumberdaya Hayati Vol. 10 No. 4 (2024): 2024
Publisher : Departemen Biologi, Institut Pertanian Bogor

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.29244/jsdh.10.4.190-197

Abstract

The increasing pollution which promotes accumulation of free radicals and a significant number of bacterial resistances to antibiotics have prompted various efforts to search for new sources of bioactive compounds. The aim of this study was to investigate the antibacterial and antioxidant properties of bioactive compounds produced by endophyte bacteria from M. bancana. The most potential bacterial isolate was extracted its active constituents until obatined crude extract and tested using disc diffusion technique and detemined MIC and MBC values. Antioxidant properties using DPPH radicals were performed followed by GC-MS analysis of its crude extract. We obtained that MB3.1 isolate exhibited antibacterial activity against all four tested bacteria including Escherichia coli strain ATCC 8739, Pseudomonas aeruginosa strain ATCC 9027, Staphylococcus aureus strain ATCC 25923, and Bacillus subtilis strain ATCC 6633. The best MIC values of this extract were shown against B. subtilis with an MIC value of 39.06 μg/ml. Further, MB3.1 extract showed a moderate antioxidant activity with an IC50 value of 248.19 μg/ml. GC-MS analysis of MB3.1 extract revealed 10 dominant compounds, including phenylethyl alcohol, benzeneethanol, 4-hydroxy, and tryptophol, which might be responsible for the antibacterial and antioxidant properties.
Evaluation of Antibacterial and Antibiofilm Effects from Soil Streptomyces spp. against Multidrug-Resistant Bacteria Prastya, Muhammad Eka; Simbolon, Sumihartati; Priyanto, Jepri Agung; Hasidu, La Ode Abdul Fajar; Permatasari, Vera; Primahana, Gian; Dewi, Rizna Triana; Kristiana, Rhesi; Suryanti, Erma
HAYATI Journal of Biosciences Vol. 32 No. 1 (2025): January 2025
Publisher : Bogor Agricultural University, Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.4308/hjb.32.1.12-26

Abstract

The global increase in multidrug-resistant (MDR) bacterial infection has rapidly gained concern globally. This study aimed to investigate antibacterial and antibiofilm potential of 25 soil actinomycete strains against MDR strains including Escherichia coli strain M4, Pseudomonas aeruginosa strain M19, Klebsiella pneumoniae strain M19, Bacillus subtilis strain M18, and Methicillin Resistant Staphylococcus aureus (MRSA). In this study, three actinomycete isolates encoded APM-7, APM-11, and APM-21 showed a strong and broad antibacterial spectrum. The minimum inhibitory concentration (MIC) of extracts derived from these isolates was ranged from 78 μg/ml to 10,000 μg/ml. In addition, The extracts also displayed significant biofilm inhibition values ranging from 6.06 to 72.4%. Based on the results, APM-21 extract had the best antibacterial and antibiofilm activities with the strongest values against MRSA. According to the nucleotide sequencing of the 16S rRNA gene, APM-7, APM-11, and APM-21 strains possessed similar identities with Streptomyces cyaneus, Streptomyces coerulescens, and Streptomyces panayensis, respectively. Based on Liquid Chromatography Tandem-Mass Spectrometry (LC-MS/MS) analysis, two antibacterial compounds, namely rancimanycin III, and enteromycin were detected in all those three extracts. Interestingly, APM-21 extract also contained two prominent antibacterial substances including paramagnetoquinone C, and caerulomycin I, suggesting their contribution to the most potential activities. Moreover, new insights were provided into a promising candidate for use in an active compound combating strategy to control MDR bacterial strain infection.
Antioxidant Activity and Phenolics of Kabau (Archidendron bubalinum) Pod Peel Extract from East Lampung on Blood Cell Male Mice Induced by Cadmium Riana, Elisa Nurma; Prastya, Muhammad Eka; Primahana, Gian; Anisa, Hida Arliani Nur; Khairani, Iffa Afiqa; Imaniar, Lisana Husna
Jurnal Ilmu Pertanian Indonesia Vol. 30 No. 2 (2025): Jurnal Ilmu Pertanian Indonesia
Publisher : Institut Pertanian Bogor

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.18343/jipi.30.2.353

Abstract

Cadmium (Cd) increases oxidative stress, which increases the likelihood of health problems. To protect the body from oxidative stress, natural antioxidants obtained from plants are required. The kabau plant (Archidendron bubalinum) is thought to have antioxidant phytochemicals. The purpose of this study was to assess the antioxidant activity and total phenolic component content of kabau pod peels, as well as their effect on the quantity of erythrocytes and leukocytes in male mice exposed to cadmium. This study used a completely randomized design, with 24 mice divided into six groups: three control groups and three treatment groups. The findings of assessing the antioxidant activity of peel extract revealed that the IC50 was 93.6 ppm, which is considered strong. The phenolic content of peel extract was 72.26 mg GAE/g. Giving pod peel extract to mice increased the number of erythrocytes and leukocytes, however the impact was not significant (p = 0.028 and P = 0.239). Mice with 380 mg/kg BW produced better outcomes. Keywords: antioxidant, Archidendron, blood cell, kabau, phenolics
Potensi Aktivitas Antibakteri dan Antioksidan dari Senyawa Bioaktif Bakteri Tanah Asal Samarinda, Kalimantan Timur: The Antibacterial and Antioxidant Potential of Bioactive Metabolites from Soil-Derived Bacteria in Samarinda, East Kalimantan Atwita, Syelziva Yonda; Malau, Jekmal; Permatasari, Vera; Primahana, Gian; Dewijanti, Indah D.; Yuswan, Apriza; Prastya, Muhammad Eka
Jurnal Sumberdaya Hayati Vol. 11 No. 2 (2025): 2025
Publisher : Departemen Biologi, Institut Pertanian Bogor

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.29244/jsdh.11.2.63-73

Abstract

Antibiotic resistance is a global challenge, especially in the treatment of bacterial infections. This study aims to explore the antibacterial and antioxidant potential of bioactive compounds isolated from soil bacteria in Samarinda, East Kalimantan. Of the 30 isolates tested, 1 potential isolate was obtained, namely isolate code T1.16. This potential bacterial isolate was further cultured and fermented in Tryptic Soybean and Luria Bertani Broth medium to obtain its crude secondary metabolite. Interestingly, its crude extract showed antibacterial activity with MIC values of 100.4-3,211 µg/ml against Escherichia coli strain ATCC 8739, Pseudomonas aeruginosa strain ATCC 9027, Bacillus subtilis strain ATCC 6633, and Staphylococcus aureus strain ATCC 25923. Based on 1,1-diphenyl-2-picrylhydrazyl (DPPH) method was used to identify antioxidant activity, IC50 values obtained ranged from 990-1730 µg/ml. Ultimately, by using Gas Chromatography Mass Spectrometry (GC-MS) analysis, bioactive compounds in the form of Cyclo(L-prolyl-L-valine), 2-methylpropyl and phenylmethyl in bacterial extract T1.16 have potential antioxidant and antibacterial bioactivities. These results support the potential development of new antibacterial and antioxidant compounds from soil bacteria, as well as the importance of further exploration for pharmacological applications.
Dammarane-Type Triterpenoids from Twigs of Aglaia Foveolata and Their Antibacterial Activity Hutagaol, Ricson Pemimpin; Mozef, Tjandrawati; Nurilmala, Febi; Primahana, Gian; Fajriah, Sofa; Prastya, Muhammad Eka; Hidayatullah, Sigit; Satyaningsih, Desy
Molekul Vol 20 No 2 (2025)
Publisher : Universitas Jenderal Soedirman

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20884/1.jm.2025.20.2.16241

Abstract

ABSTRACT. The Aglaia species, which contains triterpenoids, is the most numerous in the Meliaceae family. Aglaia foveolata (A. foveolata) is a type of plant that has many benefits, as medicinal ingredients. The potential of this plant is inseparable from the content of various bioactive compounds. This study aims to isolate, characterize the active compound from the twigs of A. foveolata and test its activity as an antibacterial. Three dammarane-type triterpenoids were isolated from the A. foveolata twigs which is, namely dammar-24-en-3β,20-diol (1), an epimeric mixture of shoreic and eichlerianic acid (2, 3). Their chemical structures were determined based on spectroscopic data using infrared, high-resolution mass spectrometry, and including one and two-dimensional NMR techniques, as well as through data comparison of the reported compound. Compound 1 was reported for the first time to be successfully isolated from this species. All these substances were tested for the first time for their antibacterial activity against two Gram-positive bacteria Staphylococcus aureus and Bacillus subtilis and two Gram-negative bacteria Escherichia coli and Pseudomonas aeruginosa, through this study. Compound 1 was inactive, the epimeric mixture of 2 and 3 showed moderate antibacterial activity with a minimum inhibitory concentration (MIC) value ranging from 31.7 to 126.6 ppm, particularly against S. aureus with a MIC value of 31.7 ppm. Keywords: Aglaia, Elucidation, Isolation, Spectroscopy, Bacterial
Evaluasi Aktivitas Antioksidan dan Antibakteri Dari Daun Empat Tumbuhan Suku Baduy Sebagai Sumber Bahan Alam Potensial Permatasari, Vera; Riskiana, Angger Arfi; Saefurohman, Asep; Prastya, Muhammad Eka; Ridha, Rafifa; Primahana, Gian; Dewijanti, Indah Dwiatmi
Jurnal Sumberdaya Hayati Vol. 11 No. 3 (2025): 2025
Publisher : Departemen Biologi, Institut Pertanian Bogor

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.29244/jsdh.11.3.122-129

Abstract

Baduy indigenous plants have great potential as natural sources of bioactive compounds, yet scientific studies on their biological activities remain limited. This study aimed to evaluate the antioxidant, antibacterial activities, and total phenolic content of the leaves from four Baduy plant species: Gardenia jasminoides, Strobilanthes crispus, Ficus callosa, and Artocarpus camansi. The leaves were extracted with methanol, followed by fractionation using n-hexane and ethyl acetate. Antioxidant activity was assessed using DPPH and ABTS assays, total phenolic content was determined by the Folin–Ciocalteu method, and antibacterial activity was tested against Staphylococcus aureus, Bacillus subtilis, Pseudomonas aeruginosa, and Escherichia coli using the broth dilution method to determine Minimum Inhibitory Concentration (MIC). The results showed that the ethyl acetate fraction of A. camansi leaves exhibited the highest total phenolic content (23.53 mg GAE/g extract). This fraction demonstrated strong antioxidant activity in the ABTS assay (IC50 = 18.55 ppm) and moderate activity in the DPPH assay (IC50 = 50.67 ppm). Moreover, it showed strong antibacterial activity against S. aureus, B. subtilis, and P. aeruginosa. These findings indicate that A. camansi leaves possess promising potential as a natural source of antioxidant and antibacterial agents.
Extracellular Metabolites of Clove-Endophytic Bacteria, Niallia nealsonii DCL1, Exhibit Potential Cellular Antioxidant and Antiaging Activities Salsabilah, Nadiyah; Prastya, Muhammad Eka; Astuti, Rika Indri
Biology, Medicine, & Natural Product Chemistry Vol 14, No 2 (2025)
Publisher : Sunan Kalijaga State Islamic University & Society for Indonesian Biodiversity

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.14421/biomedich.2025.142.747-755

Abstract

The clove plant, Syzygium aromaticum L., is a highly valued spice in Indonesia, recognized for its extensive applications in food preservation and medicinal uses. In a prior study, six endophytic bacteria were successfully isolated from clove leaves. One of these isolates, designated as DCL1, is a Gram-positive bacterium identified as Niallia nealsonii. This study aimed to determine the antioxidant and antiaging properties of the extracellular metabolite of DCL1. Extracellular metabolites were extracted with ethyl acetate and evaluated for antioxidant activity via the DPPH assay. Total phenolic and flavonoid compounds were also quantified with further metabolilte profiling using Liquid Chromatography Tandem-Mass Spectrometry (LC-MS/MS) analysis. Further study of antiaging activity was assayed using yeast model Schizosacchromyces pombe. The extract from N. nealsonii DCL1 cultures harvested at 28 and 40 hours yielded 0.01% and 0.009%, respectively. Our findings indicate that the 40-hour extract contains higher levels of phenolics (93.54 mg gallic acid equivalents/g extract) and flavonoids (12.09 mg quercetin equivalents/g extract) compared to the 28-hour extract. Additionally, the 40-hour extract exhibited greater antioxidant activity than the 28-hour extract, as demonstrated by DPPH (IC50 222 µg/mL) and ABTS (IC50 511.43 µg/mL) assays. Moreover, the 40-hour extract significantly prolonged the viability of S. pombe, suggesting its potential as an anti-aging agent. LC-MS/MS analysis identified several potential bioactive compounds, including gallic acid and N-carbamoyl-2,3-dihydroxybenzamide, which may contribute to its bioactivity. Ultimately, our study indicates that DCL1 40-h extract showed antiaging potential, which is promising as source and formulation of biopharmaceutical or cosmeceutical products. 
Eugenol Exhibits Antimutagenic Activity in Model Yeast Schizosaccharomyces pombe by Regulating DNA Damage Repair System: A Transcriptomic Analysis Muhammad Zulfiqri, Aldy; Astuti, Rika Indri; Prastya, Muhammad Eka
HAYATI Journal of Biosciences Vol. 32 No. 6 (2025): November 2025
Publisher : Bogor Agricultural University, Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.4308/hjb.32.6.1538-1550

Abstract

Free radicals that accumulate in cells can cause oxidative imbalance and trigger oxidative stress at the cellular level. Such conditions lead to damage to genetic material that accelerates cellular aging. Plant secondary metabolites have been reported to exhibit antioxidant activities that have the potential to overcome oxidative stress. Eugenol, derived from cloves (Syzygium aromaticum), has been shown to possess antioxidant activity in vitro. However, there is no information on the antioxidative activity of eugenol at the cellular level. Our study focused on the effect of eugenol as an antigenotoxic agent in preventing DNA damage caused by oxidative stress treatment. Further analysis on the cellular levels of eugenol was done through a transcriptomics study in the model yeast Schizosaccharomyces pombe. Treatment with eugenol may increase the cell viability of yeast exposed to mutagenic UV and EMS, suggesting that eugenol can act as an antigenotoxic agent. Indeed, eugenol treatment can repair DNA damage as indicated by the DNA nicking assay. Eugenol showed potential as an antiaging agent by regulating the cell cycle of S. pombe, specifically by slowing down at the G1 phase and progressing towards the S phase. Transcriptomics analysis revealed that eugenol treatment can regulate genes related to DNA damage response, specifically mismatch repair, base excision repair, and Nucleotide excision repair, in the yeast S. pombe. Our data suggest that eugenol has the potential to regulate the oxidative stress-induced DNA damage response in yeast S. pombe, which supports cell longevity.
Antibacterial Activity of Endophytic Actinobacteria Isolated from Ziziphus mauritiana Against Multidrug-Resistant Strains Khairani, Hafifatunil; Priyanto, Jepri Agung; Prastya, Muhammad Eka; Retnowati, Dwi
Makara Journal of Science Vol. 29, No. 1
Publisher : UI Scholars Hub

Show Abstract | Download Original | Original Source | Check in Google Scholar

Abstract

Increasing resistance to existing antibiotics has led to a need for new alternative sources. Among the possibilities that need to be explored is the endophytic actinobacteria associated with Ziziphus mauritiana. The present study aimed to evaluate the antibacterial activity of endophytic actinobacteria isolated from Z. mauritiana against five multidrug-resistant bacterial strains. Thirty actinobacteria isolates were obtained from the roots, stem, and leaves of the plant using the serial dilution technique. Using a double-layer agar test, we found that colonies from five of the 30 isolates (16%) exhibited antibacterial activity against methicillin-resistant Staphylococcus aureus (MRSA) and the M18 strain of Bacillus subtilis. Interestingly, the crude extracts derived from four selected isolates (BBK 1, DBK 2, DBK 3, and DBSB 1) showed inhibitory activity against MRSA, with inhibition zone diameters ranging from 8 (±0.8) to 11 (±2.9) mm. The highest inhibition zone was seen in DBK 2. This had a zone diameter of 11 (±2.9) mm, which is not signifi-cantly different from the zone diameter of 12 (±0) mm seen in tetracycline. The minimum inhibitory concentrations (MICs) were determined using the micro-broth dilution technique. This showed MICs ranging from 2,500–5,000 μg/mL in the four extracts. Based on 16S ribosomal ribonucleic acid (rRNA) sequences, the four selected isolates were found to belong to the Streptomyces spp., with similarity values exceeding 99%. These four isolates require further study as potential antibiotic sources, particularly against MRSA.
Co-Authors Anisa, Hida Arliani Nur Asep Saefurohman Atwita, Syelziva Yonda Chang, Helen Cordova, Datu Muhammad Dewijanti, Indah D. Dewijanti, Indah Dwiatmi Dwi Retnowati Febi Nurilmala Filailla, Euis Hasidu, La Ode Abdul Fajar Hening, Egiyanti Nur Widhia Hidayatullah, Sigit Hutagaol, Ricson Pemimpin Iffa Afiqa Khairani Imaniar, Lisana Husna Jekmal Malau Jepri Agung Priyanto, Jepri Agung Khairani, Hafifatunil Muhammad Zulfiqri, Aldy Permatasari, Vera Primahana, Gian Putra, Rayhan Helmyana Rhesi Kristiana Riana, Elisa Nurma Ridha, Rafifa Rika Indri Astuti Riskiana, Angger Arfi Rizna Triana Dewi Salsabilah, Nadiyah Satyaningsih, Desy Simbolon, Sumihartati Sofa Fajriah Sri Pujiyanto SUKIRNO Suryanti, Erma Tjandrawati Mozef Yuswan, Apriza Zahra, Aliya Azkia