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All Journal AL KAUNIYAH Biogenesis: Jurnal Ilmiah Biologi Biotropika Biology, Medicine, & Natural Product Chemistry Jurnal Biologi Tropis Journal of Dedicators Community Jurnal Ilmiah Fillia Cendekia JSMARTech : Journal of Smart Bioprospecting and Technology J-PEN Borneo : Jurnal Ilmu Pertanian Soeropati: Journal of Community Service Borneo Journal of Biology Education (BJBE) Biopedagogia Tri Dharma Mandiri: Diseminasi dan Hilirisasi Riset Kepada Masyarakat (Jurnal Pengabdian Kepada Masyarakat) Agrikan: Jurnal Agribisnis Perikanan Jurnal Sains Alami (Known Nature)
Ramadhan, Majida
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Religion Agriculture, Biological Sciences & Forestry Arts Humanities Biochemistry, Genetics & Molecular Biology Chemical Engineering, Chemistry & Bioengineering Chemistry Earth & Planetary Sciences Economics, Econometrics & Finance Education Environmental Science Health Professions Immunology & microbiology Materials Science & Nanotechnology Medicine & Pharmacology Neuroscience Public Health Social Sciences Veterinary Other

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Journal : JSMARTech : Journal of Smart Bioprospecting and Technology

 1 
Investigation of Total Phenol Content in Red Pomegranate Leaves Extract (Punica granatum L.) Januarista, Terra; Mubarakati, Nurul Jadid; Ramadhan, Majida
JSMARTech: Journal of Smart Bioprospecting and Technology Vol. 5 No. 1 (2024): JSMARTech Volume 5, No. 1, 2024
Publisher : JSMARTech

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.21776/ub.jsmartech.2024.005.01.09

Abstract

Red pomegranate leaves (Punica granatum L.) is one of the plants that has many benefits and potential as herbal medicine. Red pomegranate leaves have bioactivities such as alkaloids, flavonoids, phenols, saponins, tannins and terpenoids so that they can be used as an alternative antioxidant. This study aims to determine the comparison of the amount of total phenol content in red pomegranate leaves extract using maceration and digestion extraction methods. Total phenol content was determined using the Folin Ciocalteau method with a spectrophotometer UV-Vis. The research method carried out was descriptive experimental quantitative and data analysis was carried out using one way ANOVA. The results of the determination of total phenol content in gallic acid obtained a regression equation y = 0.012x + 0.545 with R2 = 0.9981. The results showed that the total phenol content in the ethanol extract of red pomegranate leaves was greater with the maceration method of 91.83 (µg GAE/g sample) compared to the digestion method of 83.27 (µg GAE/g sample). It can be concluded that the two methods used on red pomegranate leaf samples have no significant difference in total phenol content.
Exploration of Secondary Metabolites in Black Pomegranate Peel Extract (Punica granatum L.) as Antioxidants Using DPPH Method Rofiidah, Anisatul; Innaya, Allinha Yusfin; Putri, Nela Vede Rhofa; Maulida, Hilda Jihan; Dewi, Lina Purnama; Al Shofura, Nadya Raisa; Badria, Shoimatul Umi; Faiza, Hilda Wahyu; Hidayah, Ulfa; Ramadhani, Mieta Widya; Rafsanjani, Ramizard; Januarista, Terra; Faisal, Faisal; Ramadhan, Majida
JSMARTech: Journal of Smart Bioprospecting and Technology Vol. 5 No. 2 (2024): JSMARTech Volume 5, No. 2, 2024
Publisher : JSMARTech

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.21776/ub.jsmartech.2024.005.02.46

Abstract

Pomegranate (Punica granatum) is a plant that contains secondary metabolite compounds that can be used as medicinal content. Black pomegranate contains the highest secondary metabolites compared to red pomegranate and white pomegranate, the skin of the pomegranate contains flavonoids, phenols, tannins, and saponins. This study aims to determine the antioxidant activity of black pomegranate peel extract (Punica granatum L.) with the DPPH method. DPPH method is a method to determine the antioxidant activity in the sample by looking at its ability to counteract the free radical compound 2,2-diphenyl-1-picrylhydrazyl. The results of testing the antioxidant activity of black pomegranate skin (Punica granatum L.) produced an IC50 value of 47.51 ppm which can be concluded that black pomegranate skin is classified as very strong. This research is important because there has not been much research on black pomegranate, especially its skin, so there is a need for additional expansion and renewal of the species. In addition, it can be used as the main basis for the advancement of natural materials, especially in the field of health.
Characterization and Identification of Phytochemical Compounds Soursop Leaf Extract (Annona muricata L.) Amrullah, Kammal Nizam Amrullah; Ma’rifah, Shofia Jannatul; Azizah, Nur; Jamilah, Zahrotul; Safitri, Dewi Astriana; Bawani, Elen Ayu; Ramadhan, Majida; Hayati, Ari; Nafisa; Mahyantika, Seftya Putri
JSMARTech: Journal of Smart Bioprospecting and Technology Vol. 6 No. 2 (2025): Vol. 6 No. 2 (2025): JSMARTech Volume 6, No 2, 2025
Publisher : JSMARTech

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.21776/ub.jsmartech.2025.006.02.07

Abstract

Soursop leaves (Annona muricata L.) have long been used in traditional medicine in Indonesia to treat various diseases. This study aimed to characterize and identify phytochemical compounds present in soursop leaf extract. The analysis was carried out using UV-Visible spectrophotometric, FT-IR spectroscopy, and phytochemical screening to identify the presence of bioactive compounds such as alkaloids, flavonoids, and tannins. UV-Visible spectrophotometric analysis showed a characteristic of the isoflavone spectrum with two absorption peaks of 310-330 nm. FT-IR revealed strong absorption at 3380 cm-1 (O-H group), 1737 cm-¹ (C=O group), and 718 cm-1 (C-H alkene group), along with moderate absorption at 2922 cm-1 and 2852 cm-2 (C-H alkane group) and 1650 cm-1 (C=C alkene group). The total flavonoid content of the extract was 89.201 mg/g at 1000 ppm and 87.220 mg/g at 500 ppm. These findings indicate that soursop leaves are rich in flavonoids and other bioactive compounds with potential medicinal value.   Keywords: Soursop leaves, UV-Visible, FT-IR, phytochemical screening, total flavonoids
Profile of Secondary Metabolites and Study of Antibacterial Activity of Ethanol Extracts of Mulberry Fruit (Morus alba) in Inhibiting Escherichia coli and Staphylococcus aureus Seftya Putri Mahyantika; Abdoes Sjakoer, Nour; Ramadhan, Majida
JSMARTech: Journal of Smart Bioprospecting and Technology Vol. 6 No. 2 (2025): Vol. 6 No. 2 (2025): JSMARTech Volume 6, No 2, 2025
Publisher : JSMARTech

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.21776/ub.jsmartech.2025.006.02.15

Abstract

Mulberry fruit is known as a medicinal plant with various health benefits, including as an antibacterial agent. This study aims to identify the content of secondary metabolites and evaluate their antibacterial activity. The methods used in this study were experiments with qualitative and quantitative approaches. Extraction was performed using ethanol as a solvent with the maceration technique, while phytochemical analysis was conducted using specific reagent tests to detect the presence of bioactive compounds. Antibacterial activity testing was carried out using the disc diffusion method with varying concentrations of the extract at 25%, 50%, 75%, and 100%. Phytochemical analysis results showed that mulberry fruit extract contains alkaloids, flavonoids, phenols, saponins, tannins, and triterpenoids. Antibacterial activity testing against Escherichia coli produced inhibition zones of 19.25 mm, 20.33 mm, 26 mm, and 27.17 mm, respectively, while for Staphylococcus aureus, the inhibition zones were 17.58 mm, 20.08 mm, 25.75 mm, and 30.92 mm, respectively. In general, the inhibition zones formed showed an increase with increasing extract concentration. However, statistically, there was no significant difference in inhibiting both bacteria. Based on these results, mulberry fruit extract has balanced antibacterial potential against both Gram-positive and Gram-negative bacteria, making it a promising candidate for development as a broad-spectrum natural antibacterial agent. Keywords: antibacterial, Escherichia coli, mulberry, phytochemical screening, Staphylococcus aureus
Co-Authors Abdoes Sjakoer, Nour abdul wachid, abdul Achyar, Zhahrotun Khofifah Nur Ahmad Syauqi ahmad syauqi, ahmad Al Shofura, Nadya Raisa Aldina, Devana Rahma Amrullah, Kammal Nizam Amrullah, Kammal Nizam Amrullah Anggraeni, Agintha Silvya Anita Puspa Widiyana Ari Hayati Arianti, Leny Ardini Aziz, Achmad Qomarul Aziza, Nur Badria, Shoimatul Umi Bawani, Elen Ayu Dewi, Lina Purnama Eka Fitriah, Eka Erna Sulistyowati Erna Susanti Faisal Faisal Faisal Faiza, Hilda Wahyu Fatmawati Fatmawati Febrianti, Dewi Retno Fitirah, Eka Gemilang, Satria Hidayah, Ulfa Husain Latuconsina Husain, Muhammad Hafizh Ilfathoniyah, Aulia Innaya, Allinha Yusfin Jamilah, Zahrotul Januarista, Terra Jayanti, Angelina Khuluqiyyah, Wildhatu Dzati Fiqhul Mahanem Mat Noor, Mahanem Mat Mahyantika, Seftya Mahyantika, Seftya Putri Mardhiyah, Mardhiyah Maulida, Hilda Jihan Maulidya, Sabrina Dita Ma’rifah, Shofia Jannatul Mudzakaroh, Luluk Muhammad Fahmi Hidayatullah Muzayyanah, Nana Nafisa Nafisa, Nafisa Nirwana, Linggar Nour Athiroh Abdoes Sjakoer Nur Azizah Nurul Jadid Mubarokati Paramitha, Armanda Bella Prasetyo, Doni Pratiwi, Lidia Wahyu Putri, Nela Vede Rhofa Rafsanjani, Ramizard Rahmatullah, Muhammad Ali Rahmawati, Rizka Dwi Ramadhani, Lailatul Ramadhani, Mieta Widya Ratna Djuniwati Lisminingsih Rofiidah, Anisatul Rofiq, Muhammad Fadlan Rolita, Ninda Norma Safitri, Dewi Astriana Salamah, Forrela Zahwa Salsabillah, Salma Savana, Amartya Gesit Seftya Putri Mahyantika Siti Aminah Sri Wahyuni Tresna, Adira Tatus Wahidah, Shinta Nuriyah Wati, Dwi Kartika Wulandari, Rossa Martha Yaqin, Ngainul Yuana, Hera Yudi Purnomo Zaki, Siti Muzakiyah