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All Journal Jurnal Rekayasa Mesin Jurnal Ilmu Kedokteran (Journal of Medical Science) Pharmaciana: Jurnal Kefarmasian Jurnal Rekayasa Mesin Turbo : Jurnal Program Studi Teknik Mesin Al Qalam: Jurnal Ilmiah Keagamaan dan Kemasyarakatan Jurnal Literasiologi Teknomekanik Narra J Jurnal Farmanesia Indonesian Journal of Cancer Jurnal Kedokteran Hewan The International Journal of Tropical Veterinary and Biomedical Research Al-Muhith : Jurnal Ilmu Qur'an dan Hadits Jurnal Kewirausahaan dan Bisnis Jurnal Medika Veterinaria
Darmawi .
Faculty Of Engimeering, Department Of Mechanical Engineering Of Sriwijaya University
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Journal : Jurnal Kedokteran Hewan

 1 
ANTIBACTERIAL ACTIVITY AND HEMATOLOGICAL PROFILE OF RAT (Rattus norvegicus) DUE TO ADMINISTRATION OF ETHANOL EXTRACT OF SOURSOP FLOWERS (Annona muricata L.) AND Salmonella enteritidis INFECTION Zuraidawati, Zuraidawati; Sugito, Sugito; Darmawi, Darmawi; Taufiq, Taufiq
Jurnal Kedokteran Hewan Vol 14, No 2 (2020): June
Publisher : Universitas Syiah Kuala

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.21157/j.ked.hewan.v14i2.11646

Abstract

This study aimed to determine the antibacterial activity of soursop flower extract (Annona muricata L.) and hematological profile of rats (Rattus norvegicus) due to administration of soursop flower ethanol extract and Salmonella enteritidis infection. The concentrations of soursop flower ethanol extract used for the antibacterial activity test were 25%, 50%, 75%, 100%, with the antibiotic ampicillin 10 g/disk was used s a positive control (PC) and dimethyl sulfoxide (DMSO) 10% as a negative control (NC). For examination of hematological features, 15 male rat aged two months were used. All rats were divided into 5 treatment groups, each consisting of three rats. The NC group was not given soursop flower ethanol extract and S. enteritidis infection. The PC group was not given soursop flower extract but was given S. enteritidis infection. Groups P1, P2, and P3 were given ethanol extracts of soursop flower at a dose of 0.18, 0.36, and 0.72 g/rat/day peroral for a week using gastric sonde. On the following day (after administration of soursop flower extract) the first blood drawing was performed. All rats, except NC group, were then infected with S. enteritidis intraperitonially at dose of 3x108 CFU/mL dose (0.5 mL McFarland). One week after being infected with S. enteritidis, a second blood drawing was performed. The results of the antibacterial activity test showed that there was no antibacterial activity was observed since no inhibition at various concentrations was formed. The administration of soursop flower extract at various dosage levels was able to maintain the number of leukocytes but reduced the number of erythrocytes, hemoglobin concentration, hematocrit value and the number of platelets in rats; whereas S. enteritidis infection decreased all the hematologic features of lab rats.
KOSAKONIA SP. PROTEOLYTIC BACTERIA ISOLATED FROM RUMEN AND RETICULUM OF ACEH CATTLE Safika, Safika; Sari, Wenny Novita; Ademi, Gressha Vionalle; Arsih, Ulfi Widi; Darmawi, Darmawi
Jurnal Kedokteran Hewan Vol 14, No 2 (2020): June
Publisher : Universitas Syiah Kuala

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.21157/j.ked.hewan.v14i2.16426

Abstract

The aim of this study was to identify proteolytic bacteria from the ruminal and reticulum fluids of aceh cattle based on the 16S rRNA gene. Samples used were ruminal and reticulum fluids of aceh cattle slaughtered in Abattoir of Aceh Besar. Samples were diluted and cultured into Skim Milk Agar medium at 39 C for 48 hours. The morphology of bacterial colonies growth in the medium was observed. Colonies resulted in the largest clear zone were isolated and used for Deoxyribonucleic Acid (DNA) isolation, 16S rRNA gene amplification and sequencing. Theresults showed that morphology of dominant colonies was yellowish white color, round shape, position on the agar surface. The results ofphylogenetic analysis of RS1 and ReS2 isolates isolated from rumen and reticulum fluids of aceh cattle respectively had a close familialrelationship and belonged to the bacterial group of Kosakonia. Sequence homology showed isolate RS1 and ReS2 are probably either newEnterobacteriaceae species or unconfirmed species. Halo zone produced by ruminal bacteria had a wider diameter (25 mm vs 20 mm) than thatcaused by reticulum bacteria). Based on the results, RS1 (bacterium in the rumen) and ReS2 (bacterium in the reticulum) belong to similar type, namely Kosakonia sp. with a proteolytic activity. Presumably, these bacteria originate from the rumen that enters the reticulum with degraded feed.
TOTAL BACTERIA AND IDENTIFICATION OF PROTEOLYTIC RUMINAL AND RETICULUM BACTERIA FROM LOCAL KACANG GOAT Safika, Safika; Darmawi, Darmawi; Ramadhani, Fitria Sari; Nurhaspika, Nurhaspika; Moliwati, Moliwati
Jurnal Kedokteran Hewan Vol 12, No 1 (2018): March
Publisher : Universitas Syiah Kuala

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.21157/j.ked.hewan.v12i1.9288

Abstract

This study aimed to calculate the total bacteria and to identify the proteolytic bacteria in rumen and reticulum of local Kacang goat. The samples used were the rumen and reticulum fluids of five goats at abattoirs in Banda Aceh. Isolation of proteolytic bacteria was carried out using skim milk agar with pour plate method and incubated at 39 C for 48 hours. The bacterial colonies morphology was observed and the total bacterial count was recorded. DNA of the widest proteolytic index colony was isolated, amplified, and sequenced. The results showed that the dominant colonies morphology was white with position inside the agar. The average of total proteolytic bacteria in goat rumen fluid and reticulum fluid were 6.416x106 CFU/mL and 2,382x107 CFU/mL, respectively. Isolates with the widest proteolytic index was Ru3 (2.5 mm) in the rumen which homology and phylogenetic tree analysis of 16S rRNA showed 93% sequence similarity with Bacillus subtilis, while in reticulum was Re1 (2.0 mm) which has 92% sequence similarity to Tatumella. It is concluded that the number of proteolytic bacteria in reticulum is greater than the number of proteolytic bacteria in the rumen of local kacang goat. Homology analysis in this study proved that the Ru3 and Re1 isolate were probably either a new species or unconfirmed species.
Co-Authors A, Yufrizal Ademi, Gressha Vionalle Aini, Sabrina Ambiyar, Ambiyar Amiruddin Amiruddin Anas, Marnisyah Aneka Firdaus Angraini, Nani Angreini, Meliyantika Annisa, Aiza Apriyan, Dian Arenja, Regina Trisya Arman Sayuti Arsih, Ulfi Widi Arwizet Arwizet, Arwizet Awaluddin Awaluddin Beočanin, Anđelija Budi Syahri budianto panjaitan Dion, Selin Dwinna Aliza Dwisnu, Erlian Erianza, Dava Erwin Erwin Etriwati E Fadhil Fuad Rachman Ferdian, Riyan Giovani, Edo Habibie, Maulana Hasan, Denny Irmawati Hasan, Maryatun Helmi, T. Zahrial Heni Pujiastuti Husni Hidayat Irwin Bizzy Jamil, Kurnian Fitri Khairussyifa, Najmi Kurnia F. Jamil Kusuma, Herwin Wijaya Lalu Muhammad Irham Liansyah, Tita M. M Amin Indra Putra M. Nur Salim M. Syukri Ismail Maimun Syukri, Maimun Maryanti, Yossi Moliwati, Moliwati MS, Firdaus Mukamal, M. Farda Insan Muslim Akmal N. Nazaruddin Nathania, Auren Ningrum, Sania Devita Nora Usrina Nurhaspika, Nurhaspika Nuzul Asmilia Pangaribuan, Machyuddin Tumpak Marojahan Prawana, Yogi Ramadhani, Fitria Sari Razali Daud Razali, Renardy Reza S, Donel Safika S, Safika Saud, Eddy Setiawan, Sepdirama Sibuan, Sibuan Siti Aisyah Solly Aryza Sugeng Prasetyo Sugito - Suhandri, Wiwin Sukma, Zahwa Tamara Sumanto Sumanto Suparno Suparno Syafruddin Syafruddin Tabrani, Tabrani Taufiq Taufiq Ummu Balqis Usman, Said Wenny Novita Sari Winarto Winarto Wirawan Adikusuma Wiwit Ade Fidiawati Yenni Yusriani Yuliani Yuliani Zahra, Leina Putri Zamzami, Rumi Sahara Zikra, Izza Fattalia Zulkifli, Baidillah Zuraidawati Zuraidawati, Zuraidawati