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All Journal HAYATI Journal of Biosciences International Journal of Biosciences and Biotechnology METAMORFOSA Journal of Biological Sciences Jurnal Biologi Udayana Bumi Lestari SIMBIOSIS ISM (Intisari Sains Medis) : Jurnal Kedokteran Advances in Tropical Biodiversity and Environmental Sciences

Ni Putu Adriani Astiti

Program Studi Biologi, Fakultas Matematika Dan Ilmu Pengetahuan Alam, Universitas Udayana

Author-ID : 220296

Agriculture, Biological Sciences & Forestry Humanities Biochemistry, Genetics & Molecular Biology Earth & Planetary Sciences Energy Environmental Science Immunology & microbiology Medicine & Pharmacology Veterinary

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STRUKTUR HISTOLOGI HATI MENCIT(Mus musculus L.) SETELAH PEMBERIAN EKSTRAK DAUN EKOR NAGA (Rhapidhophora pinnata Schott) Ni Komang Tia Pramesti; Ngurah Intan Wiratmini; Ni Putu AdrianiAstiti
SIMBIOSIS Vol 5 No 2 (2017)
Publisher : Jurusan Biologi Universitas Udayana

ABSTRACT Rhapidhophora pinnata, Schott is a traditional medical plant that has been use remedy for treatment of hypertension, stroke. Aims of this research is to determine the effect of Rhapidhophora pinnata Schott leaf extract toward mouse (Mus musculus L) liverhistological structure. This research used completely randomized design with 28 female mice that were divide into 4 groups of P0 (control) received 0,9% NaCl, group P1, P2, and P3 received 50, 100 and 150 mg/kg bw respectively by oral administration. The treatment were given daily for 14 days. All of the mice were sacrificed by ether after 15 days. The liver were examined for their histopathological changes, namely fatty degeneration, hydropic degenaration and necrosis. Results were statistically analised by Kruskal-Wallis method. Treatment groups showed no significant differences with regard of liver histopatological changes, however hemorrhage, sinusoid congestion and inflamatory cell infiltration were found in liver. Keyword : Rhapidhophora pinnata Schott, liver, mice

USE OF MEDIA TYPES AND FERTILIZER TREATMENTS FOR THE GROWTH OF DENDROBIUM ORCHIDS Ida Ayu Meva Lianawati; Ni Putu Adriani Astiti; Ni Luh Suriani
SIMBIOSIS Vol 9 No 1 (2021)
Publisher : Jurusan Biologi Universitas Udayana

ABSTACT Reseach on dendrobium orchids was planted in the media of wood charcoal and coconut fibers and it is needed with Gandasil B fertilizer wood charcoal and coconut fiber media and is needed with Gandasil B and Multitonik fertilizer. This study aims to determine the effect of media and fertilizer on orchid growth and to determine pests and diseases in Dendrobium orchid plants. This reseach was conducted in May until July 2019 in the Plant Physiology Laboratory Biologi Prodi Studi Biologi Fakultas Matematika dan Ilmu Pengetahuan Alam Universitas Udayana. Qualitative and quantitative data are used that combine the Gandasil B fertilizer and fibers and wood charcoal media Fertilizer Gandasil B fertilizer with concetration G0 (0 g L-1), G2 (20 g L-1), G3 (30 g L-1), G4 (40 g L-1), G5 (50 g L-1). Fertilizer Multitonik M0 (0 g L-1), M1 (1,1 g L-1), M2 (1,2 g L-1), M3 (1,3 g L-1), M4 (1,4 g L-1), M5 (1,5 g L-1). Each fertilizer concentration was repeated 6 times. The results showed the best orchid growth was found in the use of Multitonik fertilizer and the best orchid growth was found in the use of Multitonik number of leavers and root growth were found in the use of coconut fibers media

AKTIVITAS ANTIDIABETES EKSTRAK ETANOL DAUN BELIMBING BESI (Averhhoa carrambola) PADA MENCIT (Mus musculus) NI PUTU SRI PUSPITA WIDI YANTHI; Ni Putu Adriani Astiti; Ni Wayan Sudatri
SIMBIOSIS Vol 9 No 2 (2021)
Publisher : Jurusan Biologi Universitas Udayana

Senyawa antioksidan, flavonoid, dan saponin banyak terkandung dalam daun belimbing besi (Averrhoa carambola) sehingga tujuan dari penelitian ini untuk melihat pengaruh antidiabetes ekstrak daun belimbing besi pada mencit. Penelitian ini menggunakan rancangan acak lengkap (RAL) dengan 6 perlakuan, yaitu P1 (aquadest tanpa aloksan) sebagai kontrol negatif, P2 (aquadest dengan aloksan) sebagai control positif, P3 metformin 500 mg/kgBB), P4 (aloksan + ekstrak daun belimbing besi 100 mg/kgBB) , P5 (aloksan + ekstrak daun belimbing besi 150 mg/kgBB), P6 (aloksan + ekstrak daun belimbing besi 200 mg/kgBB). Sebelumnya hewan coba dijadikan diabetes dengan menginduksi aloksan sebanyak 120 mg/kgBB secara intramuscular pada otot paha. Pembuatan ekstrak daun belimbing besi menggunakan metode maserasi. Setelah hewan diabetes, diberikan perlakuan ekstrak etanol daun belimbing besi sesuai rancangan percobaan, dimulai hari ke-7 sampai hari ke-21. Kadar glukosa darah diukur dengan glucometer setiap minggu. Hasil penelitian menunjukkan nilai penurunan kadar glukosa darah terbaik terjadi pada minggu pertama dan minggu kedua. Pada minggu pertama, perlakuan metformin mampu menurunkan glukosa darah mencit sebanyak 88.4%, sedangkan perlakuan ekstrak etanol daun belimbing besi dosis 200 mg sebanyak 60%. Pada minggu kedua, 65.8% untuk metformin, dan 63% untuk dosis ekstrak daun belimbing 200 mg/kg BB. Ekstrak etanol daun belimbing besi berpotensi sebagai obat antidiabetes. Kata Kunci: mencit, diabetes mellitus, glukosa darah, daun belimbing besi.

AKLIMATISASI ANGGREK HITAM (Coelogyne pandurata Lindl.) HASIL PERBANYAKAN IN VITRO PADA MEDIA BERBEDA Ni Kade Ayu Purnama Adi; Ida Ayu Astarini; Ni Putu Adriani Astiti
SIMBIOSIS Vol 2 No 2 (2014)
Publisher : Jurusan Biologi Universitas Udayana

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Black orchid (Coelogyne pandurata Lindl.) is an orchid endemic to the island ofBorneo. However, its existence is increasingly threatened with extinction. Conventionalpropagation efforts require a long time. Therefore in vitro propagation was performed. Thepurpose of this study was to determine the growth response of black orchids on the media anddifferent planting techniques. Black orchid plantlets that have been sub-cultured wasacclimatized in four different media types ie moss, fern, wood charcoal, wood charcoal andcoconut fiber mixture and different planting techniques namely compot (community pot) andthe individual, were allowed to grow for three months. Randomized block design (RBD) wasutilised, obtained 8 combination treatments, with 7 replicates. The results showed blackorchid has a good growth response in the media moss, ferns, and a mixture of wood charcoaland coconut fiber, while the wood charcoal media showed unfavorable results. Differentplanting techniques showed no significant results. Percentage of high life shown in mixedmedia wood charcoal and coconut fiber and compot techniques.Keyword : Black orchid (Coelogyne pandurata Lindl.), acclimatization, media

EFEKTIFITAS DAYA HAMBAT BAKTERI Streptomyces sp TERHADAP Erwinia sp PENYEBAB PENYAKIT BUSUK REBAH PADA TANAMAN LIDAH BUAYA (Aloe barbadensis Mill) SARMILA TASNIM; RETNO KAWURI; NI PUTU ADRIANI ASTITI
SIMBIOSIS Vol I, No 1, Tahun 2013
Publisher : Jurusan Biologi Universitas Udayana

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Streptomyces sp was conducted from December 2010 - June 2011 at the Laboratoryof Microbiology, Biology Department, Math and Science Faculty, UdayanaUniversity Bukit Jimbaran-Bali. Implementation stages of the research consisted ofisolation and testing of the antibiotic activity Streptomyces sp to inhibit growthbacterial pathogens Erwinia sp as a cause of disease in plants fallen foul (Soft rot) ofAloe barbadensis Mill.The results of this study have eight isolates of Streptomyces spwith macroscopic and microscopic characters are varied. Furthermore, all isolateswere obtained and then tested against antibiotic activity to inhibit growth the bacteriaErwinia sp. Test results obtained by Streptomyces sp that has the most effective ininhibiting the ability of the bacteria Erwinia sp isolates are Streptomyces sp2for (45%).

IDENTIFIKASI SENYAWA AKTIF DAUN PLETEKAN (Ruellia tuberosa L.) DENGAN MENGGUNAKAN GC-MS Ida Ayu Nopiari; Ni Putu Adriani Astiti; Ngurah Intan Wiratmini
SIMBIOSIS Vol 4 No 2 (2016)
Publisher : Jurusan Biologi Universitas Udayana

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ABSTRACT The use of traditional medicines in human therapies has been claimed to be much safer than that of synthetic medicines with many negative side effects. Pletekan plant (Ruellia tuberosa L.) has been used empirically for years as an antidiabetic as its leaves contain poly phenolic compounds with the ability to stimulate beta cells of the pancreas so that its capability to produce insulin improved. The main objective of our research was to identify active compounds contained in Ruellia tuberosa L. leaves extracted with ethanol. Fractionation of these active compounds was conducted by applying column and thin layer chromatography using Dichlorometan and Ethylacetat with a ratio of 6:3 as eluent. Each active compound resulted from fractionation was identified using GC-MS analysis. The results showed that four active compounds (Hexadecanamide, 9-Octadecenamide, (Z), Octadecenamide and 1,2-Benzenedicarboxylic acid were identified in this analysis. Keywords: Ruellia tuberosa L., identification of active compounds.

EKSPLORASI SPATIAL DAN IDENTIFIKASI CENDAWAN ENDOFIT PADA TANAMAN KAKAO (Theobroma cacao L.) DI BALI Lilis Riana Tambunan; Meitini Proborini; Putu Adriani Astiti
SIMBIOSIS Vol 6 No 1 (2018)
Publisher : Jurusan Biologi Universitas Udayana

Cocoa plant (Theobroma cacao L.) can grow well in the highlands and lowlands, a plantation and industrial plantwhich is known as one of the export commodities, as raw material for the food and pharmaceutical industries which cancontribute to an increase in foreign exchange endophytic fungus is a fungus living on a live network of plants such asseeds, leaves, flowers, twigs, stems and roots. Truffles are generally mutualistic endophyte and is known to stimulate thegrowth of plants, capable of producing antibiotics and plant growth regulators (hormones). This study aims to determinethe diversity and identify endophytic fungi on Kakao.yang found in two different locations in Bali, in the village of KajaBuahan District of Payangan, Gianyar and the Village of Tua, Marga, Tabanan regency subdistricts. Research carried outin the field with the exploration methods, isolation, identification and diversity analysis conducted at the Laboratory ofPlant Taxonomy (Mycology), Department of Biology, Faculty of Mathematics, University of Udayana. Laboratoryresearch conducted by isolating the roots, leaves, plant stems and content of the soil (rhizosphere) cocoa. Once isolated,grown on PDA medium subsequently incubated at room temperature 250C. Isolates that grow back isolated and purifiedon a PDA medium for further identified to the genera or species level. A total of six isolates of endophytic fungi identifiedin the rhizosphere and cocoa plant that is where four of these isolates were identified to species, two isolates were notidentified until genus. Fungi were identified namely Aspergillus flavus, Rhizopus stolonifer, Cladosporiumsphaeropermum, Trichoderma viride, isolates 1 and 2 isolates.Keywords: cocoa, endophytic fungi, highlands and lowlands

PENGARUH EKSTRAK BUNGA FLAMBOYAN (Delonix regia Hook Raf) TERHADAP PERKECAMBAHAN DAN PERTUMBUHAN CABAI MERAH BESAR (Capsicum annuum L.) Royana Pakpahan; Ni Putu Adriani Astiti; I.G.A. Sugi Wahyuni
SIMBIOSIS Vol 5 No 1 (2017)
Publisher : Jurusan Biologi Universitas Udayana

The reach aim to the effect of flamboyant (Delonix regia Hook Raf.) flower extract on the growth of large red pepper plant (Capsicum annuum L.), for use a land under of flamboyant trees. The research design used was Random Block Design, with five treatments extract concentration, control, 5%, 10%, 15%, and 20%, each treatment was repeated five time. Observations were made during the 15 weeks. The results showed the percentage of germination and plant height are effect significant, while the number of leaves, root length and dry weight of the plant does not provide effect for the growth of large red pepper plant. Keywords : flamboyant, allelopathy, large red pepper

Co-Authors A.A.S. A. Sukmaningsih AA Istri Mas Padmiswari Bernadina Metboki Deglory Tunmuni Dwi Ariani Yulihastuti ENIEK KRISWIYANTI I Gusti Ayu Sugi Wahyuni I Ketut Ginantra I Ketut Suada IDA AYU ASTARINI Ida Ayu Meva Lianawati Ida Ayu Nopiari Ida Bagus Gede Darmayasa IRIANI SETYAWATI Jeremy Pramana Kadek Ayu Trisna Yanti Lilis Riana Tambunan Luh Putu Wrasiati MADE PHARMAWATI Made Ria Defiani Meitini Proborini Wahyuni Ngurah Intan Wiratmini Ni Kade Ayu Purnama Adi Ni Komang Tia Pramesti Ni Luh Suriani Ni Made Dewi Wahyuni Ni Made Puspawati Ni Made Sastriyani Wiendra Ni Nyoman Nila Arieswari NI PUTU SRI PUSPITA WIDI YANTHI Ni Wayan Sudatri Pande Putu Purwaningsih Putu Yayun Antari Budaya Retno Kawuri Riana Dyah Suryaningrum Royana Pakpahan SANG KETUT SUDIRGA SARMILA TASNIM Yan Ramona Yuli Setiawati Yulidia Iriani Yulidia Iriani Yully Muharyati

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