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ANDRIA AGUSTA
Research Center For Biology, Indonesia Institute Of Sciences (LIPI), Cibinong, Bogor 16911, Indonesia
Agriculture, Biological Sciences & Forestry Automotive Engineering Biochemistry, Genetics & Molecular Biology Chemical Engineering, Chemistry & Bioengineering Chemistry Computer Science & IT Decision Sciences, Operations Research & Management Earth & Planetary Sciences Energy Environmental Science Health Professions Immunology & microbiology Materials Science & Nanotechnology Medicine & Pharmacology Public Health Other

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Journal : JURNAL BIOLOGI INDONESIA

 1 
Eksplorasi Keanekaragaman Aktinomisetes Tanah Ternate Sebagai Sumber Antibiotik Nurkanto, Arif; Listyaningsih, Febrianti; Julistiono, Heddy; Agusta, Andria
JURNAL BIOLOGI INDONESIA Vol 6, No 3 (2010): JURNAL BIOLOGI INDONESIA
Publisher : Perhimpunan Biologi Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (274.268 KB) | DOI: 10.14203/jbi.v6i3.3141

Abstract

ABSTRACTExploration of Soil Actinomycetes Diversity from Ternate as Indigenous Antibiotic Sources.Actinomycetes of soil samples from Ternate, North Moluccas were isolated using SDS-YEmethod in humic acid vitamin agar. Ternate has high abundance of Actinomycetes, approximately6.00 – 487 x 104 CFU/ g soil, depends on habitat types. We have selected 60 isolates andconducted antibiotic screening against pathogenic bacteria and fungi using agar diffusionmethod and found both narrow and broad antibiotic spectrum types . Based on 16S rDNAanalysis, all Actinomycetes with antibiotic activities are belong to the genus Streptomyces. .Minimum Inhibitor Concentration (MIC) value was determined by broth microdilution method.It was found that MIC values varied, depended on microbial tested. We found two isolateswith higher antibiotic activity compared to the commercial antibiotics (chloramphenicol,erythromycin for antibacterial and nystatin, kabicidin for antifungal). Cell destruction analysiscaused antibiotic activities was conducted through leak of protein and nuclatic acid.Key words : Actinomycetes, soil, Ternate, antibiotic, cell distruction
Identifikasi Molekular dan Karakterisasi Morfo-Fisiologi Actinomycetes Penghasil Senyawa Antimikroba Nurkanto, Arif; Agusta, Andria
JURNAL BIOLOGI INDONESIA Vol 11, No 2 (2015): JURNAL BIOLOGI INDONESIA
Publisher : Perhimpunan Biologi Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.14203/jbi.v11i2.2193

Abstract

ABSTRACTThe objectives of study were to identify antimicrobial producing Actinomycetes using 16S rDNA analyses and morphology and physiology characteristics. Eight Actinomycetes strain with the higest antibacterial and antifungal activity were selected and identified using six primers (20F, 520F, 920F, 1500R, 920R, and 520R). Morphological observation and physiology analyses were performed to the selected strain to accurately identify the strains. Morphological characters observed were aerial mycelium, spore chain, colony form, and pigment production. Physiological characterizations were antimicrobial properties, growth temperature, pH tolerance, salinity concentration for growth, sugars assimilation, and some enzymes production (arginine dihydrolase, urease, ß-glucosidase, protease, ß-galactosidase). Based on homology search by BLAST program and phylogenetic tree analyses, all of isolates were identified as the genus Streptomyces. They belong to eight different spesies. Isolates RC-SS-37-4, RC-SS-37-16 and BL-22-3 have been identified as Streptomyces costaricanus (100 %), Streptomyces costaricanus (99.8 %) and Streptomyces parvulus (98.6 %), respectively. Five isolates were identified as Streptomyces spp. (BL-36-1, BL-20-2, BL-14-2, BL-22-1 and BL-06-5) and can be presumed as new species because of the low homology value to their closest related spesies. Keywords : actinomycetes, antimicrobial, morphology, phylogenetic, physiology, 16S rRNA gene. 
Biotransformasi 2E-6E-Farnesol oleh Jamur Endofit Botryosphaeria sp. CA2C-3 yang Diisolasi dari Temu Hitam (Curcuma aeruginosa ROXB.) Agusta, Andria
JURNAL BIOLOGI INDONESIA Vol 9, No 2 (2013): JURNAL BIOLOGI INDONESIA
Publisher : Perhimpunan Biologi Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.14203/jbi.v9i2.173

Abstract

The objective of study was to investigate the microbial transformation reaction of 2E-6E-farnesol by the endophyticfungi isolated from temu hitam (Curcuma aeruginosa ROXB. The transformation was carried out in PDB with (2E-6E-farnesol), incubated at room temperature (25-32o C) under shaking condition at 120 rpm for two days produceda major biotransformed product. Structure elucidation based on 1D- and 13C-NMR analysis showed that the biotransformedproduct was 10,11-dihydroxi-2E-6E-farnesol. It It was verified that biotransformation reaction of 2E-6E-farnesol into 10,11-dihydroxi-2E-6E-farnesol through an intermediate 10,11-epoxi-2E-6E-farnesol.Keywords: Temu hitam; Curcuma aeruginosa; endophytic fungi; biotransformation; 2E,6E-farnesol; 10,11-dihydroxi-2E,6E-farnesol; 10,11-epoxi-2E-6E-farnesol.
Senyawa Antibakteri Daun Belawan Putih (Tristaniopsis whiteana) Handayani, Dewi; S. Achmadi, Suminar; Agusta, Andria
JURNAL BIOLOGI INDONESIA Vol 10, No 1 (2014): JURNAL BIOLOGI INDONESIA
Publisher : Perhimpunan Biologi Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (175.137 KB) | DOI: 10.14203/jbi.v10i1.334

Abstract

Isolation of antibacterial compounds from chloroform extract of belawan putih (Tristaniopsis whiteana) leaves hasbeen done. The bioautography analysis of the extract against Escherichia coli and Staphylococcus epidermidis,subsequently followed by isolation and purification of active compounds led us to obtain two pure compounds,compound 1 and 2. Futhermore, the compound 2 was identified as betulinic acid based on their spectroscopic dataincluding ultraviolet spectrum, infrared spectrum, 1D- and 2D-NMR spectra. In addition, this is the first report ofthe occurrence of this compound in the plant of Tristaniopsis whiteana and the genus of Tristaniopsis.Keywords: antibacterial, active compound, leaves, Tristaniopsis whiteana
SENYAWA ANTIBAKTERI DAUN BELAWAN PUTIH (TRISTANIOPSIS WHITEANA) Handayani, Dewi; S. Achmadi, Suminar; Agusta, Andria
JURNAL BIOLOGI INDONESIA Vol 10, No 1 (2014): JURNAL BIOLOGI INDONESIA
Publisher : Perhimpunan Biologi Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.14203/jbi.v10i1.334

Abstract

Isolation of antibacterial compounds from chloroform extract of belawan putih (Tristaniopsis whiteana) leaves hasbeen done. The bioautography analysis of the extract against Escherichia coli and Staphylococcus epidermidis,subsequently followed by isolation and purification of active compounds led us to obtain two pure compounds,compound 1 and 2. Futhermore, the compound 2 was identified as betulinic acid based on their spectroscopic dataincluding ultraviolet spectrum, infrared spectrum, 1D- and 2D-NMR spectra. In addition, this is the first report ofthe occurrence of this compound in the plant of Tristaniopsis whiteana and the genus of Tristaniopsis.Keywords: antibacterial, active compound, leaves, Tristaniopsis whiteana
EKSPLORASI KEANEKARAGAMAN AKTINOMISETES TANAH TERNATE SEBAGAI SUMBER ANTIBIOTIK Nurkanto, Arif; Listyaningsih, Febrianti; Julistiono, Heddy; Agusta, Andria
JURNAL BIOLOGI INDONESIA Vol 6, No 3 (2010): JURNAL BIOLOGI INDONESIA
Publisher : Perhimpunan Biologi Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.14203/jbi.v6i3.3141

Abstract

ABSTRACTExploration of Soil Actinomycetes Diversity from Ternate as Indigenous Antibiotic Sources.Actinomycetes of soil samples from Ternate, North Moluccas were isolated using SDS-YEmethod in humic acid vitamin agar. Ternate has high abundance of Actinomycetes, approximately6.00 ? 487 x 104 CFU/ g soil, depends on habitat types. We have selected 60 isolates andconducted antibiotic screening against pathogenic bacteria and fungi using agar diffusionmethod and found both narrow and broad antibiotic spectrum types . Based on 16S rDNAanalysis, all Actinomycetes with antibiotic activities are belong to the genus Streptomyces. .Minimum Inhibitor Concentration (MIC) value was determined by broth microdilution method.It was found that MIC values varied, depended on microbial tested. We found two isolateswith higher antibiotic activity compared to the commercial antibiotics (chloramphenicol,erythromycin for antibacterial and nystatin, kabicidin for antifungal). Cell destruction analysiscaused antibiotic activities was conducted through leak of protein and nuclatic acid.Key words : Actinomycetes, soil, Ternate, antibiotic, cell distruction
BIOTRANSFORMASI 2E-6E-FARNESOL OLEH JAMUR ENDOFIT BOTRYOSPHAERIA SP. CA2C-3 YANG DIISOLASI DARI TEMU HITAM (CURCUMA AERUGINOSA ROXB.) Agusta, Andria
JURNAL BIOLOGI INDONESIA Vol 9, No 2 (2013): JURNAL BIOLOGI INDONESIA
Publisher : Perhimpunan Biologi Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.14203/jbi.v9i2.173

Abstract

The objective of study was to investigate the microbial transformation reaction of 2E-6E-farnesol by the endophyticfungi isolated from temu hitam (Curcuma aeruginosa ROXB. The transformation was carried out in PDB with (2E-6E-farnesol), incubated at room temperature (25-32o C) under shaking condition at 120 rpm for two days produceda major biotransformed product. Structure elucidation based on 1D- and 13C-NMR analysis showed that the biotransformedproduct was 10,11-dihydroxi-2E-6E-farnesol. It It was verified that biotransformation reaction of 2E-6E-farnesol into 10,11-dihydroxi-2E-6E-farnesol through an intermediate 10,11-epoxi-2E-6E-farnesol.Keywords: Temu hitam; Curcuma aeruginosa; endophytic fungi; biotransformation; 2E,6E-farnesol; 10,11-dihydroxi-2E,6E-farnesol; 10,11-epoxi-2E-6E-farnesol.
IDENTIFIKASI MOLEKULAR DAN KARAKTERISASI MORFO-FISIOLOGI ACTINOMYCETES PENGHASIL SENYAWA ANTIMIKROBA Nurkanto, Arif; Agusta, Andria
JURNAL BIOLOGI INDONESIA Vol 11, No 2 (2015): JURNAL BIOLOGI INDONESIA
Publisher : Perhimpunan Biologi Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.14203/jbi.v11i2.2193

Abstract

ABSTRACTThe objectives of study were to identify antimicrobial producing Actinomycetes using 16S rDNA analyses and morphology and physiology characteristics. Eight Actinomycetes strain with the higest antibacterial and antifungal activity were selected and identified using six primers (20F, 520F, 920F, 1500R, 920R, and 520R). Morphological observation and physiology analyses were performed to the selected strain to accurately identify the strains. Morphological characters observed were aerial mycelium, spore chain, colony form, and pigment production. Physiological characterizations were antimicrobial properties, growth temperature, pH tolerance, salinity concentration for growth, sugars assimilation, and some enzymes production (arginine dihydrolase, urease, ß-glucosidase, protease, ß-galactosidase). Based on homology search by BLAST program and phylogenetic tree analyses, all of isolates were identified as the genus Streptomyces. They belong to eight different spesies. Isolates RC-SS-37-4, RC-SS-37-16 and BL-22-3 have been identified as Streptomyces costaricanus (100 %), Streptomyces costaricanus (99.8 %) and Streptomyces parvulus (98.6 %), respectively. Five isolates were identified as Streptomyces spp. (BL-36-1, BL-20-2, BL-14-2, BL-22-1 and BL-06-5) and can be presumed as new species because of the low homology value to their closest related spesies. Keywords : actinomycetes, antimicrobial, morphology, phylogenetic, physiology, 16S rRNA gene. 
Co-Authors A.A. Ketut Agung Cahyawan W Agus Budiawan Naro Putra AHMAD FATHONI Ahmad Fathoni Ahmad Fathoni Andi Saptaji Kamal Anita, Syahfitri Antonius Herry Cahyana Antonius Herry Cahyana ARIEF NURKANTO Arif Nurkanto Arif Nurkanto Arif Nurkanto Atit Kanti Chairul Chairul Chairul Chairul DEWI WULANSARI Dewi Wulansari Dwinna Rahmi Edi Santosa Efendi, Darda Efendi, Oscar Efendi, Sintia Frisky Efendy, Oscar Efendy, Oscar Effendi, Oscar Elsera Br Tarigan, Elsera Br Evana Evana, Evana - Farida, Yunahara Hafid, Lukman Hartutiningsih-M. Siregar HEDDY JULISTIONO Heddy Julistiono Herjuno Ari Nugroho, Herjuno Ari Inanpi Hidayati Sumiasih, Inanpi Hidayati Kamal, Andi Saptaji Kartika D. Palupi Kartika Dyah Palupi Kartika Dyah Palupi Kartika Dyah Palupi Keim, Ary P Koraag, Alfa Michael Gabriel Lestari, Vera Budi lina marlina, lina Listyaningsih, Febrianti Listyaningsih, Febrianti Marlin Megalestin Raunsai Mohammad Fathi Royyani, Mohammad Fathi Muhamad Ilyas Muhamad Ilyas, Muhamad Muhammad Ilyas Muhammad Ilyas N.L.P. Rischa Phadmacanty Octavia Siti Nurhayati Palupi, Kartika D. Pariwidjayanti, Aris Medta Pariwidjayanti, Aris Medta Partomuan Simanjuntak Phadmacanty, N.L.P. Rischa Pipit Irawati Praptiwi Praptiwi Praptiwi Praptiwi Praptiwi Praptiwi Praptiwi Praptiwi, Praptiwi, - Pratiwi Pratiwi Putra, Aldho Pramana Putri, Ade Lia R. Subekti Purwantoro Rahmat Budiarto Rahmawati Ridwan Raunsai, Marlin Megalestin Roedhy Poerwanto Rofiq, Muhamad Abdul Royani, M Fathi S. Achmadi, Suminar S. Achmadi, Suminar Siti Aisyah Sri Yuliani SUDARMONO SUDARMONO Suryati Suryati Tarigan, Elsera Br. Taruna Shafa Arzam, Taruna Shafa Tiwi, Praptiwi Wartika Rosa Farida Wellyzar Sjamsuridzal Yuliasri Jamal YULIASRI JAMAL