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All Journal International Journal of Public Health Science (IJPHS) HAYATI Journal of Biosciences Journal of Tropical Life Science : International Journal of Theoretical, Experimental, and Applied Life Sciences Majalah Kedokteran Bandung Jurnal Biosains Pascasarjana Indonesian Journal of Tropical and Infectious Disease Majalah Kedokteran Gigi Indonesia Universa Medicina Health Notions Molecular and Cellular Biomedical Sciences (MCBS) WICAKSANA: Jurnal Lingkungan dan Pembangunan Jurnal Kedokteran: Media Informasi Ilmu Kedokteran dan Kesehatan Indian Journal of Forensic Medicine & Toxicology Folia Medica Indonesiana

Yoes Prijatna Dachlan

Faculty Of Medicine, Airlangga University

Author-ID : 2278001

Agriculture, Biological Sciences & Forestry Biochemistry, Genetics & Molecular Biology Civil Engineering, Building, Construction & Architecture Dentistry Earth & Planetary Sciences Environmental Science Health Professions Immunology & microbiology Medicine & Pharmacology Neuroscience Nursing Public Health

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Induction of Plasmodium falciparum strain 2300 dormant forms by artemisinin Lilik Maslachah; Yoes Prijatna Dachlan; Chairul A. Nidom; Loeki Enggar Fitri
Universa Medicina Vol. 34 No. 1 (2015)
Publisher : Faculty of Medicine, Universitas Trisakti

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.18051/UnivMed.2015.v34.25-34

BACKGROUND The presence of Plasmodium falciparum resistance and decreased efficacy of artemisinin and its derivatives has resulted in the issue of malaria becoming increasingly complex, because there have been no new drugs as artemisinin replacements. The aims of this research were to evaluate in vitro changes in ultrastructural morphology of P. falciparum 2300 strain after exposure to artemisinin. METHODS The research used an experimental design with post test only control group. Cultures of P. falciparum 2300 strain in one control and one mutant group were treated by exposure to artemisinin at IC50 10-7 M for 48 hours. Ultrastructural phenotypic examination of ring, trophozoite and schizont morphology and developmental stage in the control and mutant group were done at 0, 12, 24, 36, 48 hours by making thin blood smears stained with 20% Giemsa for 20 minutes and examined using a microscope light at 1000x magnification. RESULTS Dormant forms occurred after 48 hours of incubation with IC50 10-7 M artemisinin in the control group. In the mutant group, dormant forms, trophozoites with blue cytoplasm and normal schizont developmental stages were seen. Ultrastructural phenotypic morphology at 0, 12, 24, 36, 48 hours showed that in the control group dormant formation already occurred with exposure to IC50 10-7 M, while in the mutant group dormant formation occurred only with exposure to IC50 2.5x10-5 M. CONCLUSION Exposure to artemisinin antimalarials in vitro can cause phenotypic morphological changes of dormancy in P. falciparum Papua 2300 strain.

The Relationship of Hemoglobin, Interleukin-10 and Tumor Necrosis Factor Alpha Levels In Asymptomatic Malaria Patients in Trenggalek, Jawa Timur, Indonesia Arif Rahman Nurdianto; Heny Arwati; Yoes Prijatna Dachlan; Dyah Ayu Febiyanti
Molecular and Cellular Biomedical Sciences Vol 3, No 1 (2019)
Publisher : Cell and BioPharmaceutical Institute

Background: Malaria is still a universal health problem, especially in tropical countries because of high morbidity and mortality rates. Infection by Plasmodium falciparum and Plasmodium vivax could result in asymptomatic disease of malaria and be found in Trenggalek, Jawa Timur. Differences in pathogenesis among affected individuals are affected by many factors, and the immune system is one of them. Among substances involved in the malarial immunity is Tumor Necrosis Factor (TNF)-α and Interleukin (IL)-10, produced by the body's defense system as the reaction to the parasite. Therefore a study was designed to detect the level of TNF-α and IL-10 in asymptomatic malaria patients.Materials and Methods: A cross-sectional study was conducted. Thirty male asymptomatic malaria subjects, age 21 to 60 years were selected. Blood from each subject was collected and the levels of TNF-α and IL-10 were analyzed using enzyme-linked immunosorbent assay (ELISA) method. Significant values considered at p<0.05.Results: There was an increased level of TNF-α with the average of 218.760 pg/µL, and an increased level of IL-10 with an average of 257.574 pg/µL in asymptomatic malaria subjects. In normal person IL-10 level is 12.6 (8.5-16.7) pg/mL and the levels of TNF-α in normal person is 0-1.5 pg/mL because they are not produce. There was a positive correlation of TNF-α with IL-10 (r=0.332; p>0.05), and positive correlation between TNF-α and the rate of hemoglobin (r=0.002; p>0.05). IL-10 was correlated negatively with the rate of hemoglobin (r=-0.363; p<0.05).Conclusion: The results from this study conclude that TNF-α and IL-10 levels increase in asymptomatic malaria subjects.Keywords: asymptomatic malaria, TNF-α, IL-10, parasite, hemoglobin

Phytoconstituent Analysis and Antibacterial Potential of Epicarp Extracts from Mature Fruits of Persea americana Mill Cyuzuzo Callixte; Dusabimana Jean Damascene; Anwar Ma'aruf; Yoes Prijatna Dachlan; Anggraini Dwi Sensusiati; Ndayisaba Daniel; Eka Nora Vitaloka Aprilia Putri Winthoko
Molecular and Cellular Biomedical Sciences Vol 4, No 2 (2020)
Publisher : Cell and BioPharmaceutical Institute

Background: World Health Organization (WHO) has reported the antimicrobial resistance as one among the ten threats to global health in 2019. The development of plant-derived antibiotics is currently considered as a modern medicine’s greatest success. Persea americana is a plant with high medicinal profile which allow its different parts to be used for therapeutic purposes. This study is aimed to determine the antibacterial potential of ethanol and chloroform extracts from epicarp of mature fruits of P. americana Mill against human pathogens.Materials and Methods: The epicarps of avocado were dried in oven and ground into powder using porcelain mortar and pestle. The powdered plant materials were extracted with both 96% ethanol and chloroform. Extracts were qualitatively screened to examine their bioactive contents and agar well diffusion method was used to analyze the antibacterial activity of extracts against both Gram-positive and Gram-negative bacteria.Results: Both solvents showed the ability to dissolve the secondary metabolites from avocado epicarps. Phytochemical screening disclosed the presence of alkaloids, proteins, terpenoids, tannins, flavonoids, steroids and phenolic compounds in ethanolic extracts and absence of flavonoids and tannins in chloroform extracts. The extracts showed the inhibition zones ranging from 14±4.5 mm to 26±2.1 mm while streptomycin demonstrated high inhibition zones ranging from 20±3.1 mm to 30 mm. The minimum inhibitory concentration (MIC) values of extracts ranges from 0.3125 mg/mL to 20 mg/mL while the MIC values for streptomycin vary from 0.25 mg/mL to 1.25 mg/mL.Conclusion: The ethanol and chloroform extracts proved to be potentially effective as natural alternative preventives to fight against various disease-causing bacteria.Keywords: antibacterial activity, ethanol extract, chloroform extract, Persea americana, Rwanda

EFEK EKSTRAK ETANOL MENIRAN (Phyllanthus niruri L) DOSIS RENDAH TERHADAP JUMLAH LIMFOSIT T CD4+ DAN IFN-γ PADA MENCIT BALB/c YANG DIINFEKSI PLASMODIUM BERGHEI ANKA Raudatul Jannah; Henyarwati Henyarwati; Suprapto Maat; Yoes Prijatna Dachlan
JURNAL KEDOKTERAN Vol 2 No 1 (2016)
Publisher : Fakultas Kedokteran Universitas Islam Al-Azhar

Show Abstract | Download Original | Original Source | Check in Google Scholar

Malaria masih menjadi masalah kesehatan masyarakat di Indonesia, di mana sekitar 80% dari malaria wilayahnya endemik, dengan 45% populasi memiliki risiko infeksi malaria. Prevalensi malaria di Indonesia masih cukup tinggi. Pemberantasan infeksi malaria masih mengalami beberapa kendala, yaitu adanya resistensi Plasmodium terhadap obat antimalaria seperti klorokuin, pirimetamin, sulfadoksin, kombinasi sulfadoksin-pirimetamin (SP) dan artemisinin-based kombinasi therapi (ACT). Penyebaran parasit Plasmodium yang resisten terhadap obat antimalaria mendorong perlunya ditemukan obat baru yang lebih efisien, antara lain pengembangan bahan alam sebagai obat herbal. Meniran merupakan salah satu tanaman yang digunakan secara tradisional oleh masyarakat untuk mengobati penyakit malaria. Pemberian ekstrak etanol meniran dapat mempengaruhi sistem imun. Penelitian ini bertujuan untuk mengetahui efek ekstrak etanol meniran dosis rendah terhadap jumlah limfosit T-CD4+ dan ekspressi interferon gamma (IFN-γ) pada mencit BALB/c yang diinfeksi P. berghei ANKA. Penelitian ini menggunakan Post Test Only Control Design. Sampel dalam penelitian ini sebanyak 25 ekor mencit, yang terdiri dari 5 kelompok. Mencit diberi Ekstrak etanol meniran dengan dosis 40 mg/kgBB dan dosis 120 mg/kgBB selama 7 hari. Dua kelompok yang diberi EEM yaitu kelompok perlakuan 1 (KP1) diberi EEM dosis 40 mg/kgBB dan kelompok Perlakuan 2 (P2) diberi EEM dosis 120 mg/kgBB dan diinfeksi P. berghei sebanyak 1x106. Kelompok kontrol positif diinfeksi Plasmodium berghei sebanyak 1x106 tanpa ekstrak etanol meniran dan kelompok negatif kontrol 1 (KN1) diberikan EEM dosis 40 mg/kgBB dan kontrol negatif 2 (KN2) diberikan EEM dosis 120 mg/kgBB tanpa diinfeksi P. berghei ANKA. Pengamatan parasitaemia dilakukan setiap hari selama 4 hari pada sediaan darah tipis yang diwarnai dengan Giemsa 10%. Jumlah sel T CD4+ dan ekspresi IFN-γ dilakukan dengan metode flocytometry. Analisis data menggunakan Oneway ANOVA. Hasil penelitian ini menunjukkan pemberian ekstrak etanol meniran dengan dosis 40 mg/kgBB dan dosis 120 mg/kgBB tidak berpengaruh terhadap jumlah sel T CD4+ pada semua kelompok perlakuan, tetapi berpengaruh secara signifikan terhadap ekspresi IFN- γ pada semua kelompok perlakuan

Gambaran Infeksi Soil Transmitted Helminth Pada Petani Di Desa Gelgel Kabupaten Klungkung putu indah budi apsari; Ni Wayan Winianti; Heny Arwati; Yoes Prijatna Dachlan
WICAKSANA: Jurnal Lingkungan dan Pembangunan Vol. 4 No. 2 (2020)
Publisher : Lembaga Penelitian, Universitas Warmadewa

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.22225/wicaksana.4.2.2020.21-30

Soil transmitted helminth (STH) infections is kind of helminth infection which transmitted by contact with warm and moist soil especially affect the farmer. The purpose of this study was to investigate the profile of STH infection and the intensity of STH infection among farmers in Gelgel Village, Klungkung, Bali. The cross sectional study was conducted in Gelgel Village, Klungkung District, Bali and obtained stool and quitionaires from farmers by informed consent. Kato-Katz technique was used to diagnose the STH infection and to determine the intensity of infection based on the number of eggs per gram feces (EPG). Total 162 stool samples were collected from farmers aged 25-80 years, 22 farmers was positive of STH infection and 140 farmers was negative of STH infection. The result showed prevalence of STH infection was 13,5%, single infection of A. lumbricoides was 1.85%, 9.26% of T. trichiura single infection, and 0.61% of Hookworm single infection. The mixed infection was detected that were 1.23% of A. lumbricoides with T. trichiura, and 0.61% of A. lumbricoides with Hookworm. STH infection is the health problem among farmer. The farmer must be admitted antihelminthic drugs for preventing and eleminating STH infection.

Epstein-Barr Virus and Malaria Interactions: Immunology Perspective Insani Budiningsih; Jaap M. Middeldorp; Yoes Prijatna Dachlan; Usman Hadi
HAYATI Journal of Biosciences Vol. 29 No. 6 (2022): November 2022
Publisher : Bogor Agricultural University, Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.4308/hjb.29.6.824-833

Epstein-Barr Virus can cause various diseases, from acute inflammatory diseases such as fatal or chronic EBV infection, infectious mononucleosis as well as lymphoid and epithelial cancer, various autoimmune diseases, and also could interact with malaria. As EBV infects 95% of the world population, and more than 30% are infected with the protozoan parasite, with more than 500,000 deaths due to malaria cases. It is important to understand how EBV dysregulates the immune system, especially when the virus is interacting with other pathogens such as malaria parasites, causing more severe conditions in certain people like Burkitt Lymphoma. This review will be informative about the mechanism of how EBV interacts with malaria parasites and how it affects the immune system. Knowledge of various cytokines triggering the immune system which may provide links to control/minimize malaria disease severity.

Microscopic-Based Trend in Imported Malaria among Workers of Trenggalek District Returning from Kalimantan Island, Indonesia During 2012 â€“ 2016 Heny Arwati; Kusmartisnawati Kusmartisnawati; Subagyo Yotopranoto; Poppy Hidayat; Yoes Prijatna Dachlan
Journal of Tropical Life Science Vol. 9 No. 1 (2019)
Publisher : Journal of Tropical Life Science

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.11594/jtls.09.01.14

Malaria in Indonesia remains a significant health problem. Trenggalek District is one of the receptive malaria areas in East Java Province, Indonesia, with mainly imported malaria from outside Java Island. The people of this district accustomed to traveling to outside Java where malaria is endemic for temporary working purposes. Usually, they have acquired malaria infection in the areas where they worked and brought to their homeland during their return to the district. The number of imported malaria cases depend on the returning season of the workers. When no workers return from outside Java malaria case is zero. During the year of 2012-2016 microscopic examination of Microscopic examination of Giemsa-stained thick blood films of the workers have been done in the working activity of Pandean Primary Health Center on their arrival from Kalimantan Island either by active or passive case detection. Microscopically positive data were reported herein to describe the trend of imported malaria in the studied areas. A total number of malaria-infected workers were 118 cases. The trend towards increasing case number yearly between 2012 and 2013 being 1.35%, then decreasing between 2013 and 2016 to only 4 cases in 2016. Different patterns of imported malaria by month were seen in each year but no specific pattern throughout the year. The Plasmodium vivax infection was predominant every year and followed by Plasmodium falciparum and mixed infection of those two species. Trends of imported malaria cases in Trenggalek district were decreased and increased irregularly in each month yearly. However, the number of cases declined annually during 2013 â€“ 2016. Antimalarial prophylaxis and insecticides prevent the workers from malaria infection.

OVERVIEW OF NUCLEAR FACTOR-KB (NF-KB) AND NON-STRUCTURAL PROTEIN 1 (NS1) IN PATIENTS WITH DENGUE FEVER IN PREMIER HOSPITAL, SURABAYA Budiutari, Ni Nyoman; Dachlan, Yoes Prijatna; Nugraha, Jusak
Indonesian Journal of Tropical and Infectious Disease Vol. 7 No. 5 (2019)
Publisher : Institute of Topical Disease Universitas Airlangga

Dengue fever (DF) is an acute viral fever caused by RNA virus that is transmitted by Aedes aegypti and Aedes albopictus mosquitoes. DF is also called viral arthropod-borne disease and is accompanied by headaches, joint and muscle pain. The main target of dengue infection is macrophages or monocytes and dendritic cells (DC). Infected DC is caused the viral replication and the endocytosis into endosomal, easier, thus inducing the activation of NF-Ä¸B transcription factor to produce proinflammatory cytokines such as Tumor Necrosis Factor-α (TNF-α), Interleukin-1 (IL-1), IL-6, IL-12 and chemokine. NF-kB is one of the transcription factors involved in the regulation of the expression of various cytokines, chemokines and anti/pro-apoptotic proteins during infection and act as indicator of disease severity. Infected DC cells are secreted NS1 protein which is the co-factor needed for viral replication and can be detected in the first eight days. The level will be higher in the initial phase of fever. The purpose of this study was to analyze the description of NF-kB and NS1 levels in the serum of patients with dengue fever through observational analytic studies through a cross-sectional approach. This study was done on 40 patients with dengue fever and 10 healthies people as negative controls. NS1 was analyzed in serum of Panbio rapid test and NF-kB level were measured by sandwich ELISA. The results are showed positive and negative NS1 results in dengue fever patients. The average NF-kB serum level in dengue fever patients was found to be higher than the control. NF-Ä¸B level in negative NS1 was higher than the NS1 positive group. It is showed that NS1 is detected both in the acute phase. The detection of NF-Ä¸B is showed the involvement of transcription factors in the development of dengue virus infection and has a protective role for host cells.

DIFFERENCES OF INTERLEUKIN-18 AND INTERLEUKIN-10 LEVELS IN RIFAMPICIN RESISTANT AND RIFAMPICIN SENSITIVE PULMONARY TUBERCULOSIS PATIENTS IN DR. SOETOMO HOSPITAL SURABAYA Riwu, Audrey Gracelia; Nugaraha, Jusak; Dachlan, Yoes Prijatna
Indonesian Journal of Tropical and Infectious Disease Vol. 8 No. 2 (2020)
Publisher : Institute of Topical Disease Universitas Airlangga

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20473/ijtid.v8i2.10959

Rifampicin is an anti-tuberculosis drug which has an efficient antimicrobial effect and the basis of a short-term treatment regimen for tuberculosis (TB) patients. Rifampicin plays an important role against the growth and slow metabolism of Bacilli M. tuberculosis. Resistance to rifampicin causes the duration of tuberculosis treatment to be longer. Interleukin-18 (IL-18) is a pro-inflammatory cytokine which plays a role in controlling the growth of M. tuberculosis through its ability to induce IFN-Î³, while Interleukin-10 (IL-10) is an anti-inflammatory cytokine which plays a role in limiting tissue damage due to the inflammatory process and maintain tissue homeostasis. IL-18 and IL-10 has an important role in explaining the different degrees of inflammation in rifampicin resistant (RR) and rifampicin sensitive (RS) pulmonary tuberculosis patients. The purpose of this study is to determine different levels of IL-18 and IL-10 in new TB patients with RR and RS. This study was a retrospective cohort study with a cross-sectional design carried out from August-November 2018 in the TB-DOTS/MDR clinic at Dr. Soetomo Hospital, Surabaya. 50 research subjects were examined and grouped into two groups, namely pulmonary TB with RR (n = 25) and pulmonary TB with RS (n = 25) based on GeneXpert examination and anti-tuberculosis drug therapy â‰¤ 1 month. IL-18 and IL-10 were measured using the ELISA Method. Differences in IL-18 and IL-10 levels between groups were analyzed using the Mann-Whitney test. The mean level of IL-18 (pg/ml) in RR and RS pulmonary TB patients were 1273.53±749.86 and 787.96 ±589.28 respectively. The mean level of IL-10 (pg/ml) in RR and RS pulmonary TB patients were 125.25±118.32 and 128.81±135.77 repectively. The mean level of IL-18 in RR and RS pulmonary TB patients were found to have a significant difference, while the mean level of IL-10 did not have a significant difference. Keywords: Interleukin-18, Interleukin-10, Tuberculosis, Rifampicin Resistant, Rifampicin Sensitive

Correlation of Total IgE Level and Intensity of Infection among Soil Transmitted Helminthiasis Farmers in Klungkung Regency, Bali, Indonesia Apsari, Putu Indah Budi; Arwati, Heny; Dachlan, Yoes Prijatna
Folia Medica Indonesiana Vol. 55, No. 2
Publisher : Folia Medica Indonesiana

Show Abstract | Download Original | Original Source | Check in Google Scholar

The phenomena of increasing total IgE level in Soil Transmitted Helminthiasis and its protective role in resistance of infection remains unclear. The purpose of this study was to determine the correlation between total IgE and the intensity of Soil transmitted helminthiasis among farmers in these area. Total 162 farmer, age ranged 25-80 years participated in this cross sectional analytic study. Kato-Katz method was used to diagnose Soil Transmitted Helminthiasis and to determined infection intensity based on egg per gram (EPG). Total IgE levels were measured by enzyme linked flourescent assay (ELFA). The results of this study showed that 13,56% farmer positive for Soil Transmitted Helminthiasis, with single infection of Ascaris lumbricoides were 1,85%, 9,26% for Trichuris trichiura, 0,61% for Hookworm and mixed infection that were 1,23% for A.lumbricoides+T.trichiura 0,61% for A.lumbricoides+Hookworm. The intensity of infection were in mild category. Total IgE level in Soil transmitted Helminthiasis group was significantly different compared with noninfected group (p<0.05, t-test). There was significant correlation between EPG and total IgE level (r=0.667, p=0.001, Spearman test). As a conclusion total IgE level was significantly correlated with EPG, this fact can be used to develop management of Soil Transmitted Helminthiasis theraphy.

Co-Authors Anwar Ma'aruf Apsari, Putu Indah Budi Apsari, Putu Indah Budi Aryati Audrey Gracelia Riwu Bambang Purwanto Budiutari, Ni Nyoman Chairul A. Nidom Chairul A. Nidom Cyuzuzo Callixte Dusabimana Jean Damascene Dyah Ayu Febiyanti Eka Nora Vitaloka Aprilia Putri Winthoko Hari Basuki Notobroto Hari Setiono Heny Arwati Hidayat, Poppy Idha Kusumawati Insani Budiningsih Ira Arundina Iswinarno Doso Saputro Jaap M. Middeldorp Jusak Nugraha Kusmartisnawati Kusmartisnawati Kusmartisnawati Kusmartisnawati, Kusmartisnawati Lilik Maslachah Loeki Enggar Fitri Muhaimin Rifa’i Muhammad Luthfi Ndayisaba Daniel Ni Wayan Winianti Nugaraha, Jusak Nur Hayati Nurdianto, Arif Rahman Poppy Hidayat putu indah budi apsari Ragu Harming Kristina Ragu Harming Kristina Raudatul Jannah Retno Indrawati Roestamadji Santi Martini Sensusiati, Anggraini Dwi Septin Mauludiyana Sillehu, Sahrir Sri Subekti Sriwijayanti Sulistyawati Subagyo Yotopranoto Subagyo Yotopranoto Sudjajadi Keman Sukmawati Basuki Suprapto Maat Usman Hadi Waode Fifin Ervina

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