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All Journal VALENSI ILMU KELAUTAN: Indonesian Journal of Marine Sciences GRADIEN Jurnal Tadris Kimiya Alotrop Jurnal Pendidikan dan Ilmu Kimia Jurnal Laot Ilmu Kelautan Hydrogen: Jurnal Kependidikan Kimia Filogeni: Jurnal Mahasiswa Biologi Journal of Fisheries & Marine
Yosie Andriani
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Agriculture, Biological Sciences & Forestry Biochemistry, Genetics & Molecular Biology Chemistry Earth & Planetary Sciences Education Immunology & microbiology Materials Science & Nanotechnology Mathematics Physics

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Antioxidant of Ethanol Extract and Toxicity of Fractions from Aspergillus unguis a Marine Sponge Symbiont of Aaptos suberitoides Masyerli, Scify Bilqis Nawafi; Efdi, Mai; Bashari, Muhammad Hasan; Untung Kurnia Agung, Mochamad; Subhan, Beginer; Efahmi, Efahmi; Andriani, Yosie; Syafrizayanti, Syafrizayanti
Hydrogen: Jurnal Kependidikan Kimia Vol. 13 No. 1 (2025): February 2025
Publisher : Universitas Pendidikan Mandalika

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.33394/hjkk.v13i1.14645

Abstract

The marine sponge Aaptos suberitoides has been shown to have anticancer properties, with clear evidence of its capacity to suppress the growth of cancer cells. However, the pharmaceutical exploration of chemicals from marine organisms causes significant environmental concerns. Aspergillus unguis, a fungal symbiont of the marine sponge A. suberitoides, has been isolated for its potential in sustainable natural products resources. This study assesses the antioxidant activity of ethanol extract and the toxicity of four levels fractions from ethanol extracts of A. unguis mycelium and these results were reported for the first time in this study. The antioxidant of ethanol extract was determined using the 2,2-Diphenyl-1-picrylhydrazyl (DPPH) method. The fractions were examined for toxicity using the Brine Shrimp Lethality Test (BSLT). The ethanol extract of A. unguis shows very strong antioxidant bioactivity (IC50 = 42.84 mg/L). The LC50 values for hexane, chloroform, ethyl acetate, and butanol fractions were determined to be 74.11 µg/mL, 93.84 µg/mL, 59.37 µg/mL, and 142.79 µg/mL, respectively. It indicates significant toxicity. These preliminary results are important knowledge for further research into the bioactivity potential of the metabolites as candidate anticancer compounds, aligning with marine pharmaceutical drug development.
Teripang, Sea Cucumber (Stichopus sp.) Fractions Induce Apoptosis in Liver Cancer Cell Line (HepG2) Andriani, Yosie; Kassim, Murni Nur Islamiah; Oksal, Efriyana; Rosmiati, Rosmiati; Bakar, Kamariah; Aziz, Nur Asniza; Syamsumir, Desy Fitrya; Kholieqoh, Alifah Husnun; Sung, Yeong Yik; Sarjono, Purbowatiningrum Ria; Hudiyanti, Dwi; Tengku Muhammad, Tengku Sifzizul
ILMU KELAUTAN: Indonesian Journal of Marine Sciences Vol 31, No 1 (2026): Ilmu Kelautan
Publisher : Marine Science Department Diponegoro University

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.14710/ik.ijms.31.1.71-78

Abstract

Cancer remains a major global health problem, prompting continuous exploration of marine organisms as sources of novel bioactive compounds. This study evaluated the cytotoxic and apoptosis-inducing activities of F9 and F10 fractions obtained from methanolic extracts of sea cucumber (Stichopus sp.) against the human hepatocellular carcinoma cell line HepG2 as an in vitro cancer model. Sea cucumber samples were extracted using methanol maceration and subsequently fractionated through Medium Pressure Liquid Chromatography (MPLC) to obtain bioactive fractions. Cytotoxic activity was assessed using the MTS (3-(4,5-dimethylthiazol-2-yl)-5-(3-carboxymethoxyphenyl)-2-(4-sulfophenyl)-2H-tetrazolium) assay after 24, 48, and 72 h of incubation to determine cell viability. Apoptotic effects were examined using Annexin V and propidium iodide (PI) staining, followed by fluorescence microscopy to evaluate morphological changes associated with apoptosis. The results demonstrated that both fractions exhibited significant cytotoxic effects on HepG2 cells in a time-dependent manner, with IC50 values of 14 µg.ml-1 for the F9 fraction and 23 µg.ml-1 for the F10 fraction after 72 h of treatment. Among the tested fractions, F9 showed stronger cytotoxic activity at lower concentrations. Morphological observations revealed typical apoptotic characteristics such as cell shrinkage, membrane blebbing, nuclear condensation, and phosphatidylserine externalization, indicating apoptosis as the predominant mechanism of cell death. These findings demonstrate that Stichopus sp. contains bioactive compounds with anticancer potential. This study highlights the importance of marine invertebrates as natural resources for biomedical applications and supports the development of marine biotechnology strategies for cancer therapy. Further investigations are needed to identify the active compounds responsible and clarify their molecular mechanisms of action.
Co-Authors Afrizal Mayub Agustiningtias, Wardah Izzati Aisyah, Siti Zanuba Andi Parenrengi Asy’ari, Mukhammad Ayu, Inna Puspa Aziz, Nur Asniza Bakar, Kamariah Bashari, Muhammad Hasan Beginer Subhan BHAKTI KARYADI Choirunnisa, Nur Fadilla Dewi Handayani Dondy Arafat Dwi Hudiyanti Efahmi, Efahmi Elfahmi Elfahmi, Elfahmi Emma Suryati Herlinah Herlinah Hermansyah Amir, Hermansyah Ismiyarto Ismitarto Jasnizat Saidin Kholieqoh, Alifah Husnun Mai Efdi Masyerli, Scify Bilqis Nawafi Mohamad Gazali Murni Nur Islamiah Kassim, Murni Nur Islamiah Nebuchadnezzar Akbar NEVIATY PUTRI ZAMANI Ngadiwiyana M.Si. S.Si. Ni Kadek Dita Cahyani, Ni Kadek Dita Nor Basid Adiwibawa Prasetya Noraznawati Ismail Novriyandi Hanif Nurhamidah Nurhamidah Oksal, Efriyana Purbowatiningrum Ria Sarjono Riezky Maya Probosari Rosmiati Rosmiati Salsabila, Salsabila Samuel Lante, Samuel Sani, Lalu M. Iqbal Sulaeman Sulaeman Sung, Yeong Yik Sutarno Sutarno Syafrizayanti, Syafrizayanti Syamsumir, Desy Fitrya Tengku Muhammad, Tengku Sifzizul Triwijayanti, Yunita Untung Kurnia Agung, Mochamad Yahya, Rodhiyah Zahrani , Arifah