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ARBUSCULAR MYCORRHIZA FUNGI AS AN INDICATOR OF SOIL FERTILITY Syibli, Muhammad Akhid; Muhibuddin, Anton; Djauhari, Syamsuddin
AGRIVITA Journal of Agricultural Science Vol 35, No 1 (2013)
Publisher : Faculty of Agriculture University of Brawijaya in collaboration with PERAGI

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.17503/agrivita.v35i1.228

Abstract

Arbuscular mycorrhizal fungi (AMF) are ubiquitous organism that forms association with the root of most terrestrial plants. AMF association also influence soil fertility through the enhancement of chemical, biological and physical content. In this study, we enumerated AMF spores from rhizosphere of Tithonia difersivolia as an indicator of soil fertility. The results showed that the most fertile soil had the highest AMF spores density. This research has confirmed that AMF has high interaction with organic carbon, organic matter, total phosphorus, cation exchange capacity, water level, soil fungi and soil bacteria. Partial regression analysis revealed the mathematic equation for their interaction. This equation used the abundant of AMF spores as an indicator for chemical, biological and physical fertility of the soil.                 Keywords: Arbuscular mycorrhiza fungi (AMF), indicator, soil fertility, Tithonia difersivolia  
SOIL FERTILITY STATUS AND SOYBEAN [Glycine max (L) Merr] PERFORMANCE FOLLOWING INTRODUCTION OF INDIGENOUS MYCORRHIZA COMBINED WITH VARIOUS NUTRIENT SOURCES INTO SANDY SOIL Astiko, Wahyu; Sastrahidayat, Ika Rocdjatun; Djauhari, Syamsuddin; Muhibuddin, Anton
AGRIVITA Journal of Agricultural Science Vol 35, No 2 (2013)
Publisher : Faculty of Agriculture University of Brawijaya in collaboration with PERAGI

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.17503/agrivita.v35i2.249

Abstract

The experiment tested four treatments, namely inoculation of soil with mycorrhiza, inoculation of soil with mycorrhiza and cattle manure, inoculation of soil with mycorrhiza and rock phosphate, and inoculation of soil with mycorrhiza and inorganic fertilizers. Soil without any inoculation was provided as control. The treatments were arranged in a Completely Randomized Design with four replications. Soil fertility status was based on the concentrations of N, P, K, and organic-C as well as soil pH. Plant performance was determined based on its ability to uptake nutrients (N, P, K, and Ca), its growth and yield. Then, the activity of mycorrhiza was measured based on total population and percentage of root infection. Overall, results of the present study showed that, compared with other treatments, inoculation of mycorrhiza into soil and amended with cattle manure significantly performed higher concentrations of N, P, K, and organic-C. This soil condition caused soybean to absorb significantly higher nutrients, grew well with higher yield compared with plant performance in other treatments. Therefore, results of this study implies that the application of mycorrhiza into soil amended with organic matter is promising to sustain soil productivity under soybean cropping system.  Keywords: Arbuscular Mycorrhiza, soil fertility, soybean yield, dryland
Growth Inhibition of Botrytis cinerea Fungus on Strawberry (Fragaria sp.) Using Kaffir Lime (Citrus hystrix) Leaf Essential Oil Emulsion Nofia, Nita; Martosudiro, Mintarto; Muhibuddin, Anton
Agro Bali : Agricultural Journal Vol 7, No 1 (2024)
Publisher : Universitas Panji Sakti

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.37637/ab.v7i1.1623

Abstract

Post-harvest damage is caused by biological factors such as the attack of Botrytis cinerea pathogenic fungi. causes gray mold rot disease to be a source of problems in strawberry production. This study aims to determine the potential of kaffir lime leaf essential oil emulsion as an antifungal against Botrytis cinerea. The fungus was isolated from strawberry fruit samples that showed symptoms of gray mold disease. Fungal isolation was carried out through the direct planting method and antifungal inhibitory ability test by food poisoned method with potato extract agar media. This experiment used a completely randomized design consisting of 6 treatments with 5 repetitions. The results showed that kaffir lime leaf essential oil emulsion contains secondary metabolites that can inhibit 100% of the growth of Botrytis cinerea pathogenic fungal colonies. Essential oil concentrations of 0.10%, 0.15%, and 0.20% had a significant effect on the growth of Botrytis cinerea pathogenic fungi.
APLIKASI CENDAWAN MIKORIZA ARBUSKULAR (CMA) DAN BOKASHI DALAM MEMINIMALISIR PEMBERIAN PUPUK ANORGANIK PADA PRODUKSI BENIH TANAMAN JAGUNG KETAN (Zea mays ceratina) Ningrum, Dhona Puspita; Muhibuddin, Anton; Sumarni, Titin
Produksi Tanaman Vol. 1 No. 5 (2013)
Publisher : Jurusan Budidaya Pertanian Fakultas Pertanian Universitas Brawijaya

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Abstract

Peningkatan produksi jagung di Indonesia kebanyakan dilakukan dengan meningkatkan dosis pupuk anorganik, akan tetapi hasil yang didapat masih rendah, sehingga perlu diupayakan suatu teknologi ramah lingkungan untuk dapat mengefektifkan pemupukan serta memperbaiki kesuburan tanah melalui pemberian bokashi dan penggunaan mikroba potensial seperti cendawan mikoriza arbuskular (CMA). Penelitian bertujuan untuk mengetahui pengaruh aplikasi CMA dan bokashi dalam meminimalisir pupuk anorganik pada produksi benih jagung ketan. Penelitian dilakukan di laboratorium HPT dan kebun percobaan Fakultas Pertanian Malang, dimulai bulan Mei sampai November 2012. Metode penelitian yang digunakan adalah rancangan acak kelompok dengan 10 perlakuan dan 3 ulangan. Perlakuan pupuk anorganik 100%, P1= bokashi+pupuk anorganik 100%, P2= bokashi+pupuk anorganik 75%, P3= bokashi+pupuk anorganik 50%, P4=CMA+pupuk anorganik 100%, P5= CMA+ pupuk anorganik 75%, P6=CMA+pupuk anorganik 50%, P7= CMA+bokashi + pupuk anorganik 100%, P8= CMA+bokashi + pupuk anorganik 75%, P9= CMA+bokashi + pupuk anorganik 50%.Hasil penelitian menunjukkan pemberian bokashi+CMA+pupuk anorganik 75%, bokashi+CMA+pupuk anorganik 100%, bokashi +CMA+ pupuk anorganik 50%, CMA + pupuk anorganik 75% dan CMA + pupuk anorganik 100% mampu meningkatkan hasil biji ton ha-1 masing-masing sebesar 56.66%, 52.22%, 50.51%, 40.27%, dan 38.57% dibandingkan tanaman dengan perlakuan yang hanya menggunakan pupuk anorganik 100%. Penambahan bokashi dan atau CMA dapat meminimalisir pemberian pupuk anorganik pada perlakuan bokashi+ pupuk anorganik 100%, bokashi+ pupuk anorganik 75%, bokashi+pupuk anorganik 50%, CMA+pupuk anorganik 100%, CMA+ pupuk anorganik 75%, CMA+pupuk anorganik 50%, CMA+bokashi+pupuk anorganik 100%, CMA+bokashi+pupuk anorganik 75%, CMA+bokashi+pupuk anorganik 50% masing-masing sebesar 32.76%, 48.55%, 53,75%, 38.57%, 55.20%, 65.36%, 52.22%, 67.50%, dan 75.26%. Kata kunci : Jagung ketan, CMA, bokashi, pupuk anorganik
Uji Antagonisme Penicillium spp. UB Forest Terhadap Patogen Penyebab Penyakit Tanaman Cabai Muhibuddin, Anton; susanti, ambar
AGROSAINTIFIKA Vol. 7 No. 1 (2024): November
Publisher : LPPM Universitas KH. A. Wahab Hasbullah

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Abstract

Cabai (Capsicum L.) merupakan komoditas tanaman yang banyak diminati oleh masyarakat di Indonesia, namun produktivitas cabai di Indonesia masih tergolong rendah karena terkendala juga oleh serangan penyakit yang mengakibatkan kehilangan hasil. Penyakit layu yang disebabkan oleh fungi patogen Fusarium sp. dan penyakit antraknosa yang disebabkan oleh fungi patogen Colletotrichum sp. merupakan penyakit penting pada tanaman cabai. Tanaman yang terserang penyakit tersebut cenderung mengalami penurunan produktivitas. Penelitian ini bertujuan untuk mengetahui efektivitas pengendalian dan mekanisme antagonisme menggunakan Penicillium spp. terhadap fungi patogen Fusarium sp. dan Colletotrichum sp. Penelitian ini dilaksanakan di Laboratorium Penyakit Tanaman Fakultas Pertanian Universitas Brawijaya mulai bulan Desember 2023 sampai dengan bulan Juni 2024. Penelitian dilakukan melalui beberapa tahapan meliputi sterilisasi alat dan bahan, pembuatan media PDA, isolasi fungi patogen, pemurnian fungi (antagonis dan patogen), identifikasi makroskopis dan mikroskopis, uji antagonis in vitro, pengamatan makroskopis dan mikroskopis, serta perhitungan persentase daya hambat. Hasil penelitian menunjukkan bahwa Penicillium spp. mempunyai efektivitas kurang dari 40% (minimum) dalam menekan pertumbuhan fungi patogen Fusarium sp. dan Colletotrichum sp. Mekanisme penghambatan yang terjadi antara Penicillium spp. terhadap Fusarium sp. adalah kompetisi dan antibiosis, sedangkan mekanisme antara Penicillium spp. terhadap Colletotrichum sp. adalah kompetisi.
Exploration and Antagonistic Test of Endophytic Fungi from Soybean (Glycine max L. Merr) With Different Resistance to Sclerotium rolfsii Zuhria, Siti Aminatuz; Djauhari, Syamsuddin; Muhibuddin, Anton
The Journal of Experimental Life Science Vol. 6 No. 2 (2016)
Publisher : Graduate School, Universitas Brawijaya

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (1134.988 KB) | DOI: 10.21776/ub.jels.2016.006.02.08

Abstract

The research aimed to determine the diversity of endophytic fungi in soybean crop with different resistance against Sclerotium rolfsii and find out their potential antagonist in controlling S.rolfsii by in vitro and in vivo. Materials used in this study were soybean with a variety of Wilis (susceptible variety) and Sinabung (resistant variety). This research was conducted at the Microbiology Laboratory of Central Laboratory of Life Science (LSIH), Brawijya University and in the trial plantation of Malang Research Institute for Food Crops, Lawang, Malang subdistrict in September 2015 until May 2016. Type of experimental design used was Complete Randomized Design with 16 treatments and three times repetitions at in vitro experiment with 16 treatments and four times repetitions at in vivo experiment. Observation on in vitro test is covering to colony morphology of fungal pathogens on PDA medium. On the test of in vivo, it was observed a disease occurrence and effectiveness rate of endophytic fungi. There are 15 species of endophytic fungi produced from isolation, namely Trichoderma sp., Aspergillus sp.2, Aspergillus sp.3, Acremonium sp.1, Acremonium sp.2, Acremonium sp.3, Acremonium sp.4, Fusarium sp.1, Fusarium sp.2, Cephalosporium sp, Microsporum sp., Penicillium sp., and unidentified fungi called W2 and W4. The highest inhibitory of endophytic fungi against S. rolfsii by Aspergillus sp.2 is 89.18% (in vitro) and 61.21% (in vivo), while Trichoderma sp. 91.88% (in vitro) and 63.29% (in vivo). Diversity index value of Wilis variety is higher than Sinabung, i.e. 1.878 and 1.606 respectively. While dominance index value of Sinabung variety is 0.2035 and Wilis is 0.1528. Keywords: Endophytic fungi, diversity, S. rolfsii.
The Effectiveness Of Addition Mycorrhizae And Fertilizer Composition On The Spinach’s Growth (Amaranthus sp) ambar susanti; Andreas Abdul Aziz; Anton Muhibuddin
JURNAL AGRONOMI TANAMAN TROPIKA (JUATIKA) Vol. 3 No. 2 (2021): Volume 3 No.2 July 2021
Publisher : LPPM UNIVERSITAS ISLAM KUANTAN SINGINGI

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.36378/juatika.v3i2.1396

Abstract

The production of organic spinach hasn’t been able to keep up with market demand, it is necessary to produce quantity and organic spinach. The research was carried out in the laboratory and greenhouse of the Agriculture Faculty, KH.A. Wahab Hasbullah University in February - May 2021. The purpose of this study was to aims to determine the effectiveness of giving mycorrhizae to spinach plants given various organic fertilizer compositions with the decomposer Trichoderma sp. The research design used was a completely randomized design, with five treatments and five replications. The treatments are; M0 (8 grams of mycorrhizae with 0% organic fertilizer as negative control), M1 (8 grams of mycorrhizae with 25% organic fertilizer), M2 (8 grams of mycorrhizae with 50% organic fertilizer), M3 (8 grams of mycorrhizae with fertilizer organic 75%), M4 (8 gr mycorrhizal with 5 grams of NPK fertilizer as positive control). The results are M2 treatment showed good growth in plant height (18.26 cm), the number of leaves (8.40 leaves), plant wet weight (3.65 gr), and root length (13.69 cm). Mycorrhizal spinach plants are effective in absorbing nutrients available in organic fertilizers 50% of the composition of the growing media, but less effective at 25%,75%, and NPK Mutiara 16:16:16 fertilizer. Organic fertilizers with local Trichoderma sp isolate decomposer are able to provide nutrients needed by spinach plants for their growth. The application of organic fertilizers with a composition of 50% to plants given mycorrhizae is possible to suppress the use of inorganic fertilizers.