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All Journal Journal of Tropical Life Science : International Journal of Theoretical, Experimental, and Applied Life Sciences Jurnal Sains dan Seni ITS Journal of Tropical Soils Jurnal Produksi Tanaman Research Journal of Life Science Journal of Tropical Plant Protection AGRIVITA, Journal of Agricultural Science Jurnal Sains Dan Teknologi (SAINTEKBU) Journal of Information Technology and Computer Engineering Inscientech: Journal of Industrial, Science and Technology Jurnal Agronomi Tanaman Tropika (JUATIKA) Agro Bali: Agricultural Journal Jumat Informatika: Jurnal Pengabdian Masyarakat Agrosaintifika: Jurnal Ilmu-Ilmu Pertanian Jurnal HPT (Hama Penyakit Tumbuhan)
Anton Muhibuddin
Jurusan Hama Dan Penyakit Tanaman, Fakultas Pertanian, Universitas Brawijaya
Agriculture, Biological Sciences & Forestry Arts Humanities Biochemistry, Genetics & Molecular Biology Chemical Engineering, Chemistry & Bioengineering Civil Engineering, Building, Construction & Architecture Computer Science & IT Control & Systems Engineering Education Electrical & Electronics Engineering Engineering Environmental Science Industrial & Manufacturing Engineering Library & Information Science Mechanical Engineering Medicine & Pharmacology Other

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EKSPLORASI JAMUR ENDOFIT DAN KHAMIR PADA TANAMAN CENGKEH (Syzygium aromaticum) SERTA UJI POTENSI ANTAGONISMENYA TERHADAP JAMUR AKAR PUTIH (Rigidoporus microporus) Rosy Husna Shofiana; Liliek Sulistyowati; Anton Muhibuddin
Jurnal HPT (Hama Penyakit Tumbuhan) Vol. 3 No. 1 (2015)
Publisher : Universitas Brawijaya

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ABSTRAK Tujuan penelitian ini yaitu untuk mengetahui jamur dan khamir yang terdapat dalam jaringan daun, batang, pangkal batang dan akar tanaman  cengkeh serta potensinya dalam menghambat pertumbuhan jamur Rigidoporus microporus penyebab penyakit jamur akar putih (JAP) pada tanaman cengkeh.Metode penelitian yang digunakan yaitu eksplorasi dan uji antagonis jamur endofit dan khamir.Berdasarkan hasil analisis ragam dan dilanjutkan dengan uji Duncan pada taraf kesalahan 0,05, diketahui bahwa hasil uji antagonis jamur endofit dengan selisih waktu 2 hari yang memiliki persentase penghambatan yang relatif besar  dibandingkan dengan perlakuan pengujian antagonis pada waktu yang sama maupun pada selisih waktu 1 hari. Presentase hambatan  yaitu pada jamur Gonatobotryum sp. 2 (89,16%), Colletotrichum sp. 2 (69,16%), Gonatobotryum sp. 2 (65,83%), dan Jamur tidak teridentifikasi (A2) (64,99%), sedangkan pada pengujian khamir terhadap jamur R. microporus, pada semua khamir tidak memiliki potensi dalam penghambatan patogen R. microporus, karena hanya menghasilkan nilai hambatan dibawah 50%. Kata kunci : Jamur Endofit, Khamir, Rigidoporus microporus
PENGARUH FAKTOR ABIOTIK KIMIA TANAH TERHADAP SUPRESSIFITAS TANAH DALAM MENGENDALIKAN PENYAKIT LAYU BAKTERI (Ralstonia solanacearum) PADA TANAMAN TOMAT (Lycopersicon esculentum Mill) Dhani Galih Rahmawanto; Anton Muhibuddin; Luqman Qurota Aini
Jurnal HPT (Hama Penyakit Tumbuhan) Vol. 3 No. 2 (2015)
Publisher : Universitas Brawijaya

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ABSTRAK Sifat kimia tanah telah diketahui dapat mempengaruhi perkembangan penyakit tanaman. Penelitian ini bertujuan untuk mengetahui pengaruh sifat kimia tanah terhadap perkembangan penyakit layu pada tanaman tomat (L. esculentum Mill) yang disebabkan oleh Ralstonia solanacearum dan pertumbuhan tanaman tomat. Tanah endemik dan non endemik penyakit layu bakteri diperoleh dari delapan lokasi berbeda berdasarkan indikator tinggi tempat dan persentase penyakit. Penelitian dilakukan dua tahap yaitu analisis sifat kimia tanah dan percobaan rumah kaca. Percobaan rumah kaca dilakukan dengan Rancangan Acak Lengkap terdiri dari delapan perlakuan dan empat ulangan. Parameter pengamatan meliputi: perkembangan persentase penyakit layu, populasi  R. solanacearum, tinggi tanaman, berat basah dan berat kering tanaman. Hasil Percobaan diketahui bahwa sifat kimia tanah berpengaruh pada perkembangan R. solanacearum dan pertumbuhan tanaman tomat. Sifat kimia tanah terutama kandungan K, P, dan C-organik berpengaruh terhadap perkembangan persentase kejadian penyakit  layu bakteri yang disebabkan oleh R. solanacearum pada tanaman tomat. Sifat kimia tanah terutama kandungan nitrogen juga mempengaruhi pertumbuhan dan perkembangan tanaman tomat.   Kata kunci : faktor abiotik, unsur hara, Ralstonia solanacearum, tomat 
EKSPLORASI JAMUR FILOPLANE PADA TANAMAN SELEDRI (Apium graveolens) DAN UJI KEMAMPUAN ANTAGONISNYA TERHADAP PENYAKIT ANTRAKNOSA (Colletotrichum sp.) Ernita Lovera Pratiwi Pasaribu; Ika Rochdjatun Sastrahidayat; Anton Muhibuddin
Jurnal HPT (Hama Penyakit Tumbuhan) Vol. 4 No. 1 (2016)
Publisher : Universitas Brawijaya

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Penelitian ini bertujuan untuk mengkaji keberadaan jamur filoplane pada daun seledri dan kemampuan antagonisnya dalam menekan pertumbuhan Colletotrichum sp. penyebab penyakit antraknosa pada seledri. Penelitian ini dilakukan di Laboratorium Penyakit Tumbuhan, Jurusan Hama dan Penyakit Tumbuhan, Fakultas Pertanian, Universitas Brawijaya, Malang, pada bulan Maret 2014 hingga Januari 2015. Metode eksplorasi digunakan untuk mendapatkan jamur filoplane pada daun seledri, sedangkan metode oposisi langsung digunakan untuk mengetahui daya hambat jamur filoplane terhadap patogen Colletotrichum sp.. Dari hasil penelitian didapatkan 31 jenis jamur filoplane yang teridentifikasi ke dalam 18 genus yaitu Alternaria sp., Aspergillus spp. (4 isolat), Cylindrocarpon spp. (2 isolat), Curvularia sp., Dreshclera sp., Fusarium spp. (6 isolat), Gloeosporium sp., Nigrospora spp. (3 isolat), Penicillium spp. (4 isolat), Pestalotia sp., Rhizoctonia sp., Stemphylium sp., Trichoderma sp., Verticillium sp., sedangkan terdapat tiga jenis jamur filoplane yang tidak teridentifikasi. Seluruh isolat  jamur filoplane memiliki potensi sebagai antagonis karena dapat menekan pertumbuhan Colletotrichum sp. Hasil uji antagonis terhadap Colletotrichum sp., jamur Aspergillus sp. isolat 3 dan Trichoderma sp. menunjukkan daya hambat tertinggi mencapai masing-masing 68% dan 67%.
Identification of Aflatoxigenic Fungi in Myristica fragrans using V8 and CAM Media Arifah, Fitri; Aini, Luqman Qurata; Muhibuddin, Anton; Dianti, Sylvia; Prabowo, Matheus Randy
Research Journal of Life Science Vol 9, No 3 (2022)
Publisher : Direktorat Riset dan Pengabdian Masyarakat, Universitas Brawijaya

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.21776/ub.rjls.2022.009.03.3

Abstract

Nutmeg is a spice that might be susceptible to being infected with aflatoxigenic fungi. Aflatoxins are mycotoxins synthesized by certain strains of Aspergillus section Flavi. However, not all strains are able to produce aflatoxins. The approach frequently employed for this survey comprises the cultivation of strains in a suitable liquid or solid medium and their subsequent extraction and analysis for the presence of aflatoxins. To date, very few studies have been conducted on the identification of contaminate and aflatoxigenic fungi in Myristica fragrans. Therefore, this study aimed to identify the contaminate and aflatoxigenic fungi in Myristica fragrans growing on V8 and CAM Media. Isolation of the fungus was carried out by direct agar plating. Lasiodiplodia theobromae, Aspergillus niger, A. tamarii, Penicillium citrinum, Rhizopus delemar, A. nomiae, and A. aflatoxiformans were recovered from nutmeg kernels sampled from North Minahasa, North Sulawesi, Indonesia. The findings of fungal isolation demonstrated that L. theobromae was the most prevalent form of fungus detected in the nutmeg kernels (50%), followed by A. niger (38.13%) and A. tamarii (10%). A. nomiae and A. aflatoxiformans were the aflatoxigenic fungi isolated from M. fragrans kernels.
Analysis of Chitinase Enzyme Trichoderma sp. in Degrading Fusarium oxysporum Rosyida, Risya; Martosudiro, Mintarto; Muhibuddin, Anton
Research Journal of Life Science Vol 9, No 3 (2022)
Publisher : Direktorat Riset dan Pengabdian Masyarakat, Universitas Brawijaya

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.21776/ub.rjls.2022.009.03.5

Abstract

The chitinase enzyme in Trichoderma sp. play an important role in pathogen control. This is because chitin is the main component of the fungal cell wall which the chitinase enzyme can degrade. One Trichoderma sp. isolate was obtained (UBPK6) with the highest percentage of inhibition against Fusarium oxysporum, 76.71%. In addition, UBPK6 isolates also showed the highest bromocresol purple reagent test results (indicating the presence of chitinase enzymes). Chitinase enzyme activity was measured for Trichoderma asperellum isolates. The results showed that the optimum incubation period for enzyme production was the 4th day, with an enzyme activity value of 4.05 U/mL. It indicates that this time is the right time for harvesting enzymes. Furthermore, the effect of pH on the chitinase enzyme activity of Trichoderma asperellum fungus. The test results showed that the optimum value was produced at pH 5 with a value of 3.4 U/mL and decreased afterward. The pH five treatment was the best in inhibiting the growth of pathogens, with an inhibition value of 60.63%. The higher the content of the chitinase enzyme, the higher its ability to degrade damage to the germination of pathogenic spores, thus causing the growth of the pathogen to be inhibited.
Uji Antagonisme Penicillium spp. UB Forest Terhadap Patogen Penyebab Penyakit Tanaman Cabai Muhibuddin, Anton; susanti, ambar
AGROSAINTIFIKA Vol. 7 No. 1 (2024): November
Publisher : LPPM Universitas KH. A. Wahab Hasbullah

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Abstract

Cabai (Capsicum L.) merupakan komoditas tanaman yang banyak diminati oleh masyarakat di Indonesia, namun produktivitas cabai di Indonesia masih tergolong rendah karena terkendala juga oleh serangan penyakit yang mengakibatkan kehilangan hasil. Penyakit layu yang disebabkan oleh fungi patogen Fusarium sp. dan penyakit antraknosa yang disebabkan oleh fungi patogen Colletotrichum sp. merupakan penyakit penting pada tanaman cabai. Tanaman yang terserang penyakit tersebut cenderung mengalami penurunan produktivitas. Penelitian ini bertujuan untuk mengetahui efektivitas pengendalian dan mekanisme antagonisme menggunakan Penicillium spp. terhadap fungi patogen Fusarium sp. dan Colletotrichum sp. Penelitian ini dilaksanakan di Laboratorium Penyakit Tanaman Fakultas Pertanian Universitas Brawijaya mulai bulan Desember 2023 sampai dengan bulan Juni 2024. Penelitian dilakukan melalui beberapa tahapan meliputi sterilisasi alat dan bahan, pembuatan media PDA, isolasi fungi patogen, pemurnian fungi (antagonis dan patogen), identifikasi makroskopis dan mikroskopis, uji antagonis in vitro, pengamatan makroskopis dan mikroskopis, serta perhitungan persentase daya hambat. Hasil penelitian menunjukkan bahwa Penicillium spp. mempunyai efektivitas kurang dari 40% (minimum) dalam menekan pertumbuhan fungi patogen Fusarium sp. dan Colletotrichum sp. Mekanisme penghambatan yang terjadi antara Penicillium spp. terhadap Fusarium sp. adalah kompetisi dan antibiosis, sedangkan mekanisme antara Penicillium spp. terhadap Colletotrichum sp. adalah kompetisi.
Effect of Temperature on the Germination and Spread of the Mycelia of Sporisorium scitamineum Fungus Causes Smut Disease in Sugarcane Tasya, Tasya; Muhibuddin, Anton; Sektiono, Antok Wahyu; Hidayah, Nurul
Research Journal of Life Science Vol 11, No 1 (2024)
Publisher : Direktorat Riset dan Pengabdian Masyarakat, Universitas Brawijaya

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.21776/ub.rjls.2024.011.01.3

Abstract

Sugarcane is presumed to be susceptible to smut disease caused by a basidiomycete fungus, Sporisorium scitamineum. One environmental condition that influences the disease's development is temperature. However, information related to smut disease, especially the influence of the environment (temperature) on the growth of S. scitamineum in sugarcane, is limited. Therefore, studying the effect of different temperature ranges on S. scitamineum growth is necessary. The research, which was arranged using a completely randomized design with 3 (replicates) consisted of two treatments, including temperatures (20, 25, 30, and 35°C) and incubation time (1, 2, 4, and 6 hours). The teliospore suspension was inoculated on a water agar medium and incubated at different times and temperatures. After each allocated time, the plates were taken from the incubator, and then germination of teliospores was observed under a compound microscope. In addition, we also conducted research on the effect of inoculation temperature (25, 30, and 35°C) on the mycelial distribution within sugarcane tissue. The bud setts were immersed in teliospore suspension for 10 minutes at different temperatures, then planted in the polybags and kept for one and two weeks. The results showed that temperature affected the germination of S. scitamineum teliospores, in which the highest germination (92,3%) occurred at 30°C. On the other hand, the lowest germination was at 20°C with 0% germination after 1 hour incubation time. The in vivo study revealed that the distribution of mycelia within sugarcane tissue was similar, either incubated in one or two weeks.
Implementing Backward Chaining Method in Expert System to Detect and Treat Rice, Chilli, and Corn Plant’s Pests and Diseases Muhibuddin, Anton; Airlangga, Primaadi; Sulthoni, M. Mirza; Wicaksana, Aries Budi
JITCE (Journal of Information Technology and Computer Engineering) Vol. 2 No. 02 (2018)
Publisher : Universitas Andalas

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.25077/jitce.2.02.71-75.2018

Abstract

Indonesia is an agrarian country which most of it’s citizens are farmer. There are various kind of decent plants to grow in Indonesia but most of them plant rice, corn, wheat, and sago as staple food. There other plants that is consumed not as main food but as subsidiaries, one of them are chilli pepper. Abundant variety of vegetation in Indonesia means that there are also many type of pests and diseases. An agriculture expert is needed to identify plant’s diseases and pest correctly. Meanwhile, the number of agriculture experts is insufficient to help massive amount of farmer whom have trouble in agriculture. This problem can be solved by using Expert System where farmers can detect the problem on their plants and finding treatment to cure their plants correctly them self. This research is mean to develop an Expert System Application in agriculture to identify diseases and pests based on their symptoms especially rice, chilli, and corn plants. This Expert System is based on web program using Backward Chaining Inversion which is deemed necessary to solve the case study’s problem. Responsive Web Based System can be accessed by user or client from many devices especially their smart phone help diagnose disease that occurs to their plant, more over, to solve the low rate of expert on agriculture.
The Role of Indigenous Mycorrhiza in Combination with Cattle Manure in Improving Maize Yield (Zea Mays L) on Sandy Loam of Northern Lombok, Eastern of Indonesia Astiko, Wahyu; Sastrahidayat, Ika Rochdjatun; Djauhari, Syamsuddin; Muhibuddin, Anton
JOURNAL OF TROPICAL SOILS Vol. 18 No. 1: January 2013
Publisher : UNIVERSITY OF LAMPUNG

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.5400/jts.2013.v18i1.53-58

Abstract

A glass house study was conducted to evaluate the contribution of indigenous arbuscular mycorrhiza fungi (AMF) in improving maize yield grown on sandy loam of Northern Lombok. The package of organic fertilizers treatments were tested including: without inoculation of mycorrhiza, inoculation mycorrhiza and no added inorganic fertilizers, inoculation of mycorrhiza with cattle manure added, inoculation of mycorrhiza with rock phosphate added and inoculation mycorrhiza with inorganic fertilizers. The treatments were arranged using a Completely Randomized Design with four replications. The results of the study show that the inoculation of AMF significantly increased soil concentration of N, available-P, K and organic-C by 37.39%, 60.79%, 66.66% and 110.15% respectively observed at 60 days after sowing (DAS). The similar trend was also found at 100 DAS, where those nutrients increased by 21.48%, 69%, 43.93% and 37.07%, respectively compared to control. The improving of soil fertility status was also reflected by nutrients uptake (i.e. N, P, K, Ca) as well as growth and yield of maize. N, P, K and Ca uptake increased by 1,608%, 1,121%, 533% and 534%, respectively. Roots and top dry biomass at 60 DAS increased by 718.40% and 337.67%, respectively. The trend increased of the biomass was followed by observation at 100 DAS. Yield components including cobs, grain and weight of 100 grains increased by 313.60%, 411.84% and 137.54%, respectively. In addition, the inoculation of AM with F2 contributed significantly to the spore numbers and root infection.[How to Cite : Astiko W, IR Sastrahidayat, S Djauhari, and A Muhibuddin. 2013. The Role of Indigenous Mycorrhiza in Combination with Cattle Manure in Improving Maize Yield (Zea Mays L) on Sandy Loam of Northern Lombok, Eastern of Indonesia. J Trop Soils, 18 (1): 53-58. doi: 10.5400/jts.2013.18.1.53][Permalink/DOI: www.dx.doi.org/10.5400/jts.2013.18.1.53]
Control of "Damping Off" Disease Caused by Sclerotium Rolfsii Sacc. using Actinomycetes and Vam Fungi on Soybean in The Dry Land Based on Microorganism Diversity of Rhizosphere Zone Rochjatun, Ika; Djauhari, Syamsuddin; Saleh, Nasir; Muhibuddin, Anton
AGRIVITA Journal of Agricultural Science Vol 33, No 1 (2011)
Publisher : Faculty of Agriculture University of Brawijaya in collaboration with PERAGI

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.17503/agrivita.v33i1.37

Abstract

One of the obstacles in the efforts to increase soybean production in Indonesia is disease such as damping off which is caused by Sclerotium rolfsii. In East Java, the intensity of S. Rolfsii reached approximately 8.61% that spread all over Indonesia region, even in our neighbor countries such as Malaysia, Thailand and the Philippines. This research was carried out to determine the efficacy of Actinomycetes and VAM (Vesicular Arbuscular Mycorrhizal) against damping-off attack and the diversity of microorganisms in rhizosfer. Research conducted in the laboratory and screen house on Plant Protection Department, Faculty of Agriculture, University of Brawijaya and in Lawang District Malang. Observation variables include level of pathogen attack and infection rate of dampingoff pathogen. Plant height, number of pods, pod weight, seed weight and weight of 100 seeds from each treatment, diversity and identification of microorganisms in rhizosphere were also observed. The results showed that Actinomycetes and VAM application could decrease the percentage of plant death due to damping-off. Application of Actinomycetes and VAM gave effect on microorganism diversity of Ratai Rhizosphere but not on Wilis. Keywords: Sclerotium rolfsii, vesicular arbuscular mycorrhizal, actinomycetes, and rhizosphere
Co-Authors Abdul Cholil Abdul Latief Abadi Ahmad Ilham Tanzil Ahmad Ilham Tanzil Airlangga, Primaadi Akhmat Riza Faizah ambar susanti Andik Setiawan Andreas Abdul Aziz Ari Kristini Arifah, Fitri ashar, salim AthoÂ’illah Ahmad Athoillah Ahmad Birtha Niken Pratiwi Birtha Niken Pratiwi Chintya Ivana Situmorang Dhani Galih Rahmawanto Dhani Galih Rahmawanto Dhewyangga Bismi Panglipur Dhona Puspita Ningrum Dian Wulandari DIAN WULANDARI Dianti, Sylvia Edi Priyo Utomo Edi Priyo Utomo Ernita Lovera Pratiwi Pasaribu Goetz, Peter Ika Rocdjatun Sastrahidayat Ika Rochdjatun Sastrahidayat Ika Rochdjatun Sastrahidayat Ika Rochjatun Ina Mustika Dewi Indana, Izzatul istiqomah istiqomah Istiqomah Istiqomah Liliek Sulistyowati Liliek Sulistyowati Luaili Addina LuÂ’aili Addina Luqman Qurata Aini Luqman Qurata Aini Luqman Qurota Aini Luqman Qurota Aini Mei Sintia, Khusnul Mintarto Martosudiro Muhammad Akhid Syibli Nasir Saleh Nasir Saleh Nasirudin, Mohamad Nofia, Nita Novia Dwi Putri Nur Musyarrofatul Mala NURUL HIDAYAH Nurwijayanti Prabowo, Matheus Randy Pramudita, Oki Punnapayak, Hunsa Ria Kurniasih Ria Kurniasih Rizkiana Intan Pratiwi Rizkiana Intan Pratiwi Rosy Husna Shofiana Rosy Husna Shofiana Rosyida, Risya Sektiono, Antok Wahyu Sevy Dwi Kartikasari Sholihah, Dewi Maratus Sifaunajah, Agus Siti Aminah Sri Nurhatika Sulthoni, M. Mirza Susanti, Ambar Syamsuddin Djauhari Syamsuddin Djauhari Syamsuddin Djauhari Syamsuddin Djauhari Tasya, Tasya Titin Sumarni Virgien Margi Nirwana Wahyu Astiko Wibowo, Kartika Novitasari Wicaksana, Aries Budi Zakaria, Ari Viqri Zulfikar Zulfikar