Ida Ayu Pasti Apsari
Laboratorium Parasitologi Veteriner, Fakultas Kedokteran Hewan Universitas Udayana, Jl. PB. Sudirman Denpasar Bali, Indonesia 80225.

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Journal : Jurnal Medik Veteriner

Adaptation of African Swine Virus in Non-Swine Cell Lines: A Preliminary Study for Vaccine Candidate Tenaya, I Wayan Masa; Agustina, Kadek Karang; Suada, I Ketut; Apsari, Ida Ayu Pasti; Sari, Tri Komala; Handayani, Ni Made; Widayantari, Anak Agung Ayu Sauca Sunia; Suardana, Anak Agung Komang; Sumarya, I Made; Arsana, I Nyoman; Sudiartawan, I Putu; Wahyudi, I Wayan; Juliasih, Ni Ketut Ayu; Sudaryati, Ni Luh Gede; Damriyasa, I Made
Jurnal Medik Veteriner Vol. 8 No. 1 (2025): April
Publisher : Universitas Airlangga

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20473/jmv.vol8.iss1.2025.114-123

Abstract

African Swine Fever (ASF) is among the most detrimental infectious viral diseases in pigs causing approximately 100% mortality. The disease was first reported about 83 years ago in Africa before spreading to Europe in 1957 and Asia in 2010. An adequate vaccine generally containing live attenuated virus isolates prepared in swine macrophages to control the disease is currently unavailable. Therefore, this study aimed to use murine neuroblastoma (N2a) cells, non-swine cell lines, to adapt African swine fever virus (ASFV) isolates for vaccine preparation. ASFV isolate called BL21 obtained from Bali and East Nusa Tenggara was previously propagated in swine macrophages. However, virus was currently adapted in the N2a cells to avoid unwanted issues associated with using swine macrophages, including microbial contamination, as well as technically laborious and ethical issues. The adapted BL21 was re-confirmed with quantitative polymerase chain reaction (q-PCR) and tested in vivo to examine the pathogenicity properties. The results showed that BL21 produced consistently and specifically positive q-PCR, killing experimental pigs with typical gross pathological changes of ASF. BL21 at a 10-3/mL dilution adapted in N2a cells showed similar antigenic properties causing the death of nearly 50% N2a cells in vitro and terminating all in vivo experimental pigs. In conclusion, the BL21 isolate reported in this study could be used as a vaccine candidate after more attenuation and particularly to determine a lethal dose of 50% (LD50) for future investigations.
Co-Authors Adven Three Any Joy Simamora, Adven Three Any Aini, Hanifah Alshofa Nurul Anak Agung Komang Suardana Anak Agung Sagung Indraswari Anak Agung Sagung Kendran Andi Azhary Azmy, Andi Azhary Ariawan, Kadek Yudha Aryitahlia, Ninis Chandra, Michele Dina, Putu Ayu DWI SURYANTO Dwipayana, I Putu Gilang Aria Endah Rahmawati Fuady Muslih Gusti Ayu Mayani Kristina Dewi Gusti Ayu Yuniati Kencana Handayani, Ni Made Handojo, Chindi Meilina Here, Rischi Robinson Male Hutapea, Maria Anastasia I Gusti Made Krisna Erawan I Gusti Ngurah Kade Mahardika I Ketut Berata I Ketut Suada I Made Damriyasa I Made Dwinata I Made Merdana I MADE SURAGA ARTA I NYOMAN ADI SURATMA I Nyoman Arsana, I Nyoman I NYOMAN MANTIK ASTAWA I Nyoman Suarsana I Nyoman Suartha I Nyoman Sulabda I Putu Sudiarta I Wayan Masa Tenaya I Wayan Masa Tenaya, I Wayan Masa I Wayan Wahyudi, I Wayan Ida Bagus Kade Suardana Ida Bagus Komang Ardana Ida Bagus Made Oka Ida Bagus Ngurah Swacita Ida Bagus Oka Winaya Ida Bagus Windia Adnyana Iwan Haryono Utama Kadek Karang Agustina Kencana, Gst Ayu Yuniati Ketut Ayu Cudemani Putri Komang Yogie Suryana Putra Leksana Aditya Kris Nugraha Madani, Inggrid Maria Mentari Ginting, Maria Mentari Mersy Rambu Maramba Ndiha Monica Lewinsky Muliani, Ni Kadek Ni Ketut Ayu Juliasih Ni Ketut Suwiti Ni Luh Gede Sudaryati Nina Aryani Pabala, Meiksilano Ferdy Pamungkas, Pandu Adjie Prawira, Satria Yuda Putu Agus Trisna Kusuma Antara Raisis Farah Dzakiyyah Al-Aliyya Saputri, Megawati Sianturi, Inti Sari Pati R U Simamora, Saruedi Sri Kayati Widyastuti Suastini, Ni Ketut Sudiartawan, I Putu Sugiyarto - Sumartono - Sumartono . Sumartono s Sumartono s Sumarya, I Made Suryani, Ni Made Putri Tjokorda Sari Nindhia TRI KOMALA SARI Wayan Tunas Artama Wayan Tunas Artama Widayantari, Anak Agung Ayu Sauca Sunia Widyasari, Ni Nyoman Ayu Yoshihiro Hayashi Zefanya, Fiorencia