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All Journal Journal of Tropical Life Science : International Journal of Theoretical, Experimental, and Applied Life Sciences Jurnal Kimia (Journal of Chemistry) METAMORFOSA Journal of Biological Sciences Buletin Udayana Mengabdi Jurnal Biologi Udayana SIMBIOSIS BIOEDUSCIENCE Jurnal Biologi Tropis Makara Journal of Science Acitya Wisesa: Journal of Multidisciplinary Research
Ni LUH ARPIWI
Program Studi Biologi Fakultas Matematika Dan Ilmu Pengetahuan Alam Universitas Udayana
Agriculture, Biological Sciences & Forestry Biochemistry, Genetics & Molecular Biology Chemistry Civil Engineering, Building, Construction & Architecture Computer Science & IT Education Environmental Science Immunology & microbiology Physics

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POTENSI EKSTRAK BAWANG PUTIH (Allium sativum L.) DALAM MENGHAMBAT PERTUMBUHAN JAMUR Colletotrichum acutatum PENYEBAB PENYAKIT BUSUK PADA BUAH CABAI MERAH (Capsicum annuum L.) PASCA PANEN Fania Agustini; Ida Bagus Gede Darmayasa; Ni Luh Arpiwi
SIMBIOSIS Vol 10 No 2 (2022)
Publisher : Jurusan Biologi Universitas Udayana

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (157.518 KB) | DOI: 10.24843/JSIMBIOSIS.2022.v10.i02.p08

Abstract

This study aims to find out the potency of garlic extract (Allium sativum L.) to the growth of Colletotrichum acutatum fungus which cause rot of red chili fruit post-harvest. The treatments were the administration of garlic extract in-vitro and in-vivo. In the in-vitro test, the inhibition of garlic extract was measured using well diffusion method. The in-vivo test was conducted by measuring the diameter area of C. acutatum on red chilies after treatments. The concentration of garlic extract given was 15%(b/v), 20%(b/v), 25%(b/v), 30%(b/v), and 35%(b/v) with positive control of synthetic fungicides and negative control of ethanol solvents 95% and 4 times replications. The average diameter result on the 25%(b/v) concentration of bland power test had the most effective bland zone diameter of 20.1±0.08. Statistically different was real (P?0.05) with negative controls. While in vivo tests the average area of infection area at a concentration of 35%(b/v) had an average diameter area of infection area which was best in inhibiting the growth of C. acutatum fungus.
Selection of High Oil Yielding Trees of Millettia pinnata (L.) Panigrahi, Vegetative Propagation and Growth in the Field Ni Luh Arpiwi; I Made Sutha Negara; I Nengah Simpen
Journal of Tropical Life Science Vol. 7 No. 3 (2017)
Publisher : Journal of Tropical Life Science

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.11594/jtls.07.03.11

Abstract

Millettia pinnata (L.) Panigrahi is a potential legume tree that produces seed oil for biodiesel feedstock. The initial step for raising a large-scale plantation of the species is selection of high oil yielding trees from the natural habitat. This is followed by vegetative propagation of the selected trees and then testing the growth of the clone in the field.  The aim of the present study was to select high-oil yielding trees of M. pinnata, to propagate the selected trees by budding and to evaluate the survival and growth of budded plants in the field. Pods were collected from 30 trees in Lovina Beach, Buleleng Regency, Bali. Oil was extracted from seeds using soxhlet with hexane as a solvent.  The high oil yielding trees were propagated by budding using root stocks grown from M. pinnata seeds.  Scions were taken from young branches of selected trees. Incision was made on rootstock and the same size of cut was made on a scion containing a single bud.  The scion was inserted to the incision of rootstock then closed tightly using plastic strips.   The plastic was removed when the scion grew into a little green shoot. One month after plastic removal, the scion union grew into a single shoot and then the budded plants were removed to polybags. Budded plants were planted in the field of Bukit Jimbaran, Badung Regency, Bali with 4 × 4 spacing. Results showed all budded plants successfully grow new shoots. Two months after planting the survival of budded plants was 100%. Plant height increased by 22.13 cm, stem diameter increased by 2.43 mm and the number of compound leaf increased by 2.08.  It can be concluded that four high oil yielding trees were selected from Lovina Beach and successfully propagated by budding. Survival of budded plants was 100% with vigorous growth.
Analyze Of Cloves (Syzygium aromaticum L.) Leaves Essential Oil Grow From Different Elevations Range Luh Gede Artha Saridewi Wijaya; Ni Luh Arpiwi; Ida Ayu Astarini
Metamorfosa: Journal of Biological Sciences Vol 9 No 2 (2022)
Publisher : Prodi Magister Ilmu Biologi, Fakultas MIPA, Universitas Udayana

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.24843/metamorfosa.2022.v09.i02.p15

Abstract

ABSTRACT A research was carried out to determine the yield, content of constituent compounds, and analysis of the quality of clove leaf essential oil (Syzygium aromaticum L.) at different altitudes. The study used dry clove leaves obtained from Buleleng Regency, Bali Province with an altitude of 300 meters above sea level (masl), 600 masl, and 900 masl. Extraction of essential oils was carried out by steam distillation. The essential oil obtained was analyzed using Gas Chromatography-Mass Spectrometry (GC-MS) and the quality was tested using the quality requirements of SNI 06-2387-2006. Parameters observed were yield, identification of constituent compounds, analysis of essential oil quality including color, odor, specific gravity, and refractive index, solubility in alcohol, optical rotation, total eugenol and caryophyllene. The results showed that clove leaf essential oil in the lowlands (300 masl) had a higher yield of 2.05% and more types of active compounds than in the optimal altitude (600 masl) which was 1.45% and the highlands (900 masl) was 1.85%. The main compounds that make up clove leaf essential oil were eugenol, caryophyllene, and humulene. Other compounds were longifoline, nootkatone, naphthalene, citronellal, and cyclohexene. The clove leaf essential oil from the three heights met the quality requirements of SNI 06-2387-2006. Keywords: Essential oil, cloves, eugenol, GC-MS, secondary metabolites
Daya proteksi minyak atsiri bunga kenanga (Cananga odorata ) dalam sediaan lotion antinyamuk terhadap Aedes aegypti Putu Ayudina Asti Puspita; Ni Luh Arpiwi; Ni Wayan Sudatri
Jurnal Biologi Udayana Vol 26 No 2 (2022): JURNAL BIOLOGI UDAYANA
Publisher : Program Studi Biologi, Fakultas Matematika dan Ilmu Pengetahuan Alam, Universitas Udayana

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.24843/JBIOUNUD.2022.v26.i02.p12

Abstract

Berbagai alternatif telah dilakukan untuk menghambat populasi nyamuk Aedes aegypti penyebab penyakit demam berdarah, diantaranya penggunaan lotion antinyamuk yang mengandung DEET. Untuk mengurangi resiko dan dampak negatif dari lotion antinyamuk yang mengandung DEET, maka salah satu upaya dilakukan pemanfaatan minyak atsiri kenanga (Cananga odorata) sebagai repellent atau antinyamuk alami. Penelitian ini bertujuan untuk mengetahui rendemen minyak atsiri bunga kenanga, untuk menganalisis konsentrasi minyak atsiri pada sediaan lotion yang memberikan daya proteksi tertinggi terhadap gigitan nyamuk Ae. aegypti, menganalisis tingkat kesukaan probandus terhadap formulasi lotion, dan untuk mengetahui ada tidaknya sensitivitas setelah memakai lotion yang mengandung minyak atsiri bunga kenanga. Minyak atsiri kenanga pada penelitian ini diperoleh dengan cara destilasi uap yang selanjutnya digunakan sebagai agen antinyamuk dalam sediaan lotion. Rata-rata rendemen minyak atsiri bunga kenanga dengan tiga kali ulangan diikuti dengan standar deviasi (SD) adalah 0,63% ± 1,38 b/b. Daya proteksi lotion antinyamuk tertinggi adalah lotion F3 dengan konsentrasi minyak atsiri 5%. Lotion F3 pada jam pertama memberikan daya proteksi sebesar 96,3%, jam kedua 92,3%, jam ketiga 87% dan menurun sampai jam keenam menjadi 75%. Formulasi yang paling disukai oleh probandus adalah lotion dengan kandungan minyak atsiri sebanyak 5% b/b. Setelah pemakaian lotion antinyamuk tidak ada kesan lengket dikulit dan tidak adanya gejala sensitivitas.
Komunikasi Singkat: Analisis kualitas minyak atsiri daun Melaleuca trichostachya Lindl. dan daya hambatnya terhadap jamur Candida albicans Komang Ayu Mirayanti; Ni Luh Arpiwi; I Putu Agus Hendra Wibawa
Jurnal Biologi Udayana Vol 26 No 2 (2022): JURNAL BIOLOGI UDAYANA
Publisher : Program Studi Biologi, Fakultas Matematika dan Ilmu Pengetahuan Alam, Universitas Udayana

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.24843/JBIOUNUD.2022.v26.i02.p13

Abstract

Melaleuca trichostachya Lindl. merupakan tumbuhan yang termasuk famili Myrtaceae yang berasal dari Benua Australia. Daun dari tumbuhan ini mengandung minyak atsiri. Salah satu manfaat dari minyak atsiri yaitu sebagai anti jamur. Penelitian bertujuan mengetahui rendemen minyak atsiri daun M. trichostachya, kualitas, kandungan senyawa, serta daya hambatnya terhadap jamur Candida albicans. Daun M trichostachya segar dan kering sebanyak 200 g diekstraksi dengan metode destilasi uap dengan 3 kali ulangan. Rendemen minyak atsiri dihitung, kualitas diuji, senyawa penyusun dianalisis dengan Gas Chromatography Mass Spectrometry (GC-MS). Daya hambat minyak atsiri terhadap C. albicans diuji dengan kertas cakram pada media PDA dengan konsentrasi 25% b/b. Hasil penelitian menunjukkan bahwa rendemen minyak atsiri M. trichostachya daun segar adalah 1,00±0,058% sedangkan rendemen daun kering lebih rendah, yaitu 0,77±0,038%. Kualitas minyak atsiri yang dihasilkan termasuk kategori baik dari segi organoleptik, kemurnian, dan bilangan asam. Senyawa utama terdiri dari eucalyptol dan alpha terpinolene serta memiliki daya hambat terhadap jamur C. albicans.
Formulasi Body Mask dari Daun Pegagan (Centella asiatica L.) untuk Perawatan Kulit Stareast Wulandari Sigiro; Ni Luh Arpiwi; Ni Made Susun Parwanayoni
Metamorfosa: Journal of Biological Sciences Vol 10 No 1 (2023)
Publisher : Prodi Magister Ilmu Biologi, Fakultas MIPA, Universitas Udayana

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.24843/metamorfosa.2023.v10.i01.p15

Abstract

Body mask yaitu satu diantara kesediaan kosmetik yang dipakai dalam proses akhir saat melakukan perawatan kulit. Perawatan kulit terkhususnya dalam kulit tubuh membutuhkan kosmetika yang sifatnya menimbulkan lembab. Oleh karena itu perlu dilakukan perawatan agar dapat menjaga kulit supaya selalu bersih serta sehat, salah satunya dengan perawatan kulit yaitu dengan body mask. Body mask memiliki banyak manfaat yaitu memberikan kelembaban, membuat kulit menjadi kencang, memberikan nutrisi pada kulit, membuat lembut kulit, membuat bersih pori-pori dan mencerahkan warna kulit. Satu diantara bahan alam yang bisa dipakai dalam melakukan perawatan kulit yaitu daun pegagan (Centella asiatica L.). Ekstrak pegagan memiliki salah satu senyawa kandungan yaitu asiaticosida yang dapat berkhasiat menstimulasi kolagen pada jaringan kulit. Penelitian yang dilakukan mempunyai tujuan agar dapat mengetahui kandungan fitokimia daun pegagan, mengetahui formulasi sediaan masker bubuk daun pegagan yang baik, serta mengetahui formulasi terbaik menurut probandus. Metode yang dipakai saat melakukan penelitian yang dilaksanakan yaitu uji fitokimia, pembuatan body mask, uji pH, uji organoleptik, dan uji hedonik. Penelitian yang dijalankan ini menghasilkan uji fitokimia golongan steroid, saponin, flavonoid, dan tanin diperoleh hasil positif. Kualitas body mask yang dihasilkan yaitu pH 5,41, dan tidak menimbulkan iritasi kulit. Uji organoleptik tekstur halus, aroma green tea, warna putih kehijauan. Formulasi body mask yang paling disukai oleh probandus adalah menggunakan basis yang ditambah 45 g (F2) bubuk daun pegagan. Kata Kunci : Body mask, daun pegagan (Centella asiatica L.), perawatan kulit.
THE EFFECTS OF LACTIC ACID BACTERIA GROWTH INHIBITORS OF STAPHYLOCOCCUS EPIDERMIDIS SUPPLEMENTATION ON THE QUALITY OF ANTI-MICROBIAL LOTION Zulfa, Rozifatul; Ramona, Yan; Arpiwi, Ni Luh
Acitya Wisesa: Journal of Multidisciplinary Research Vol. 3 Issue 1 (2024)
Publisher : jfpublisher

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.56943/jmr.v3i1.581

Abstract

Staphylococcus epidermidis infections of the skin can cause boils, itching and long-lasting sores. Currently, around 75% to 90% of S. epidermidis infection cases are resistant to the antibiotic methicillin. Thus, this becomes a concern in the health sector. This research aims to discover a new method to treat S. epidermidis infection by using lactic acid bacteria as its opponent, so that the use of antibiotics can be reduced. The physical test includes confirmation of Lactic Acid Bacteria (LAB), inhibition test of lab on S. epidermidis, lotion formulation, organoleptic and homogeneity test of lotion, lotion hedonic test, LAB total plate count test in lotion, and data analysis using Microsoft Excel, Statistical Program Service Solution (SPSS), and ANOVA test. Lactic acid bacteria isolates were obtained from previous research stocks and tested for their ability to inhibit S. epidermidis in vitro. These lactic acid bacteria were added to the lotion. The favorability level of consumers to the lotion was also tested. The results indicated that lactic acid bacteria were able to inhibit the growth of S. epidermidis and remained effective until the seventh day. In addition, respondents provided a positive response to the physical quality of the lotion that contained lactic acid bacteria.
Production and Characterization of Biodiesel from Malapari Seed Oil (Pongamia pinnata L.): Evaluation of Quality Parameters Based on the Indonesian National Standard (SNI) 04-7182-2015 Taebenu, Desi Adriyanti Nina; Arpiwi, Ni Luh; Astarini, Ida Ayu
Makara Journal of Science
Publisher : UI Scholars Hub

Show Abstract | Download Original | Original Source | Check in Google Scholar

Abstract

This study produced biodiesel from Malapari seed oil (Pongamia pinnata L.) by optimizing the esterification and transesterification processes. The objectives of this study were to determine the sulfuric acid (H2SO4) catalyst concentration in the esterification reaction that can reduc the free fatty acid (FFA) content of the seed oil to ≤2%, analyze the oil to methanol molar ratio (i.e.,1:6, 1:9, and 1:12) and KOH concentration (i.e.,2%, 4%, and 6%) that will produce the highest biodiesel yield, and evaluate the quality of the produced biodiesel according to the Indonesian National Standar (SNI) 04-7182-2015. The research was conducted from October to December 2023 at the Plant Physiology Laboratory and the Physical Chemistry Laboratory, FMIPA, UNUD. The research applied Completely randomized design. Quantitative biodiesel data were analyzed using Analysis of Variance. If significant differences (α < 0.05) were detected, then the quantitative biodiesel data were further analyzed using Duncan's Post Hoc Test. Results showed that an H2SO4 concentration of 7% reduced the FFA content to 1.915%. A molar ratio of 1:12 with a 2% KOH catalyst produced the highest biodiesel yield of 65.35%. The produced met the SNI for the saponification number (i.e., 52.82–133.69 mg-KOH/g), iodine number (i.e.,7.19–22.42 g_I2/100_g), and cetane number (i.e., 86.48–132.68). However, the water content (i.e., 0.721%–2.407%), viscosity (i.e., 1.73–6.18 mm²/s), acid number (i.e., 0.224–4.685 mg_KOH/g), density (i.e., 874–917 kg/m³), FFA content (i.e., 0.116 -% to 2.229%), and methyl ester content (48,13% to–132.12%_mass) partially did not meet the standards. This study highlights the potential of Malapari seed oil as a biodiesel feedstock, with further required needed to meet all quality standards.
Produksi Kompos dari Bungkil Biji Malapari (Pongamia pinnata L.) Taebenu, Desi Adriyanti Nina; Arpiwi, Ni Luh; Astarini, Ida Ayu
BIOEDUSCIENCE Vol 9 No 1 (2025): BIOEDUSCIENCE
Publisher : Universitas Muhammadiyah Prof. Dr. Hamka

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.22236/jbes/14480

Abstract

Background: Biodiesel production from malapari seeds generates waste in the form of seed cake that can still be utilized. This seed cake can be processed into compost because it contains various essential minerals for plant growth. This study analyzes the quality of compost made from malapari seed cake based on the SNI 19-7030-2004 standard. Methods: The compost fertilizer was fermented for 31 days using four treatments. (P1) malapari cake 1kg + Orgadec 2g; (P2) malapari cake 334g + goat manure 333g + burnt rice husk 333g + Orgadec 2g; (P3) Malapari cake 200g + goat manure 400g + burnt rice husk 400g + Orgadec 2g; (P4) Malapari cake 400g + goat manure 200g + burnt rice husk 200g + Orgadec 2g. Results: Water, phosphorus, and potassium content tests in treatments P1, P2, P3, P4 met specifications. pH tests in treatments P1, P2, P4 met specifications, P3 did not meet specifications. Nitrogen tests in P1 and P2 met specifications, but P3 and P4 did not. The organic C test in P1 did not meet specifications, while P2, P3, and P4 met specifications. The electrical conductivity test in P1 met specifications, while P2, P3, and P4 did not. Color and odor in P1 did not meet specifications, while P2, P3, and P4 met specifications. Conclusions: Compost from malapari seed cake has met the compost quality specifications according to SNI 19-7030-2004 for P2, so it is suitable for plant application.
Utilization of Black Rice Var Wojalaka with Lemongrass Essential Oil as a Face Mask Ghello, Jennilien Merinda; Arpiwi, Ni Luh; Sukmaningsih, A. A. S. Alit
Jurnal Biologi Tropis Vol. 25 No. 2 (2025): April-Juni
Publisher : Biology Education Study Program, Faculty of Teacher Training and Education, University of Mataram, Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.29303/jbt.v25i2.8944

Abstract

Black rice contains antioxidants that neutralize free radicals, helping prevent wrinkles and skin damage. Fragrant lemongrass produces essential oils with citronellal, geraniol, and citronellol, which have antibacterial effects. This study aims to develop and evaluate a face mask combining black rice extract and citronella essential oil to nourish, cleanse, and tighten the skin. Masks were formulated with black rice concentrations of 30%, 40%, and 50%. Evaluations included phytochemical screening, antioxidant activity (DPPH assay), organoleptic properties, pH, homogeneity, dispersibility, and physical stability. Black rice extract showed antioxidant content of 0.037 mg/L, while the mask formulations exhibited strong antioxidant activity at 2.231 mg/L. The FII mask met Indonesian National Standard (SNI) criteria for physical stability. Clinical tests from week 2 to 6 on volunteers revealed that the FIII mask improved skin firmness, reduced acne and black spots, and increased moisture. This research demonstrates that combining black rice antioxidants with citronella essential oil produces an effective natural face mask that enhances skin health.
Co-Authors . Ramdhoani Anak Agung Ketut Darmadi Andy Agustina Lande Barnadus Nggahu Lindi Djawa Dwi Ariani Yulihastuti Elizabeth L Barbour Erlina Adhayanti Fania Agustini Ferliana Febritasari GUIJUN YAN I Gusti Ayu Sugi Wahyuni I Ketut Ginantra I KETUT MUKSIN I Made Sutha Negara I Made Sutha Negara I Nengah Simpen I Putu Agus Hendra Wibawa I.G.A.S. Wahyuni IDA AYU ASTARINI Ida Bagus Gede Darmayasa Jennilien Merinda Ghello Julie A Plummer Komang Ayu Mirayanti Luh Gede Artha Saridewi Wijaya M. Arsa M. Indra Dwitama MADE PHARMAWATI Made Ria Defiani MADE SUSUN PARWANAYONI Maria Lorita Amfotis Ni G. A. M. Dwi Adhi Suastuti Ni Luh Cipta Ayumi Nellawati Ni Luh Made Yuli Indra Yanti Ni Luh Suriani Ni Made Rai Suarni NI NYOMAN DARSINI Ni Putu Harta Diani Pande Ni Wayan Sudatri Putu Ayudina Asti Puspita Retno Kawuri Rosa Ichi Harum Pratiwi SANG KETUT SUDIRGA Stareast Wulandari Sigiro Sukmaningsih, A. A. S. Alit Taebenu, Desi Adriyanti Nina Yan Ramona Zulfa, Rozifatul