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All Journal AQUATIC SCIENCE & MANAGEMENT Media Teknologi Hasil Perikanan Jurnal e-Biomedik JURNAL PESISIR DAN LAUT TROPIS PHARMACON JURNAL ILMIAH PLATAX JURNAL BIOMEDIK JURNAL LPPM BIDANG SAINS DAN TEKNOLOGI Briliant: Jurnal Riset dan Konseptual Medical Scope Journal (MSJ)
Robert A. Bara
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Agriculture, Biological Sciences & Forestry Biochemistry, Genetics & Molecular Biology Civil Engineering, Building, Construction & Architecture Computer Science & IT Earth & Planetary Sciences Education Environmental Science Health Professions Immunology & microbiology Languange, Linguistic, Communication & Media Medicine & Pharmacology Nursing Public Health

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Journal : JURNAL ILMIAH PLATAX

 1 
Isolation and Screening the Symbiont Bacteria of the Sponge Dragmacidon sp from Manado Bay, North Sulawesi that Producing Chitinase and Protease Sembiring, Sindiy Cloudya; Warouw, Veibe; Wullur, Stenly; Bara, Robert A; Salaki, Meiske S.; Ginting, Elvy Like
Jurnal Ilmiah Platax Vol. 9 No. 1 (2021): ISSUE JANUARY - JUNE 2021
Publisher : Sam Ratulangi University

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.35800/jip.9.1.2021.34307

Abstract

Enzymes are important in the technology industry and hydrolytic enzymes, such as chitinase and protease are commonly used for it. Various types of microorganisms such as bacteria can produce hydrolytic enzymes.  Sponge-associated bacteria are excellent sources of extracellular hydrolytic enzymes because the surface and internal spaces of sponges are richer in nutrients. The aim of this study was to isolate and screen the bacteria of the sponge Dragmacidon sp symbiotic from Manado Bay, North Sulawesi that producing chitinase and protease   Symbiont bacteria were grown in Zobell 1226 E medium with a dilution of 10-4. Bacterial isolation was carried out based on the morphological characteristics of the colony. Chitinase and protease activity was carried out by growing each bacterial isolate in chitin and protein media at 36oC for 48 hours. Chitinase and protease activities were indicated by the formation of a clear zone around the bacterial colony, however, the clear zone for chitinase activity was observed after pouring the Lugol's solution. Based on this study, 8 isolates bacteria of the symbiotic spongy Dragmacidon sp from Manado Bay, North Sulawesi were isolated based on morphological characteristics. The colony of the bacteria is generally white with an irregular shape. Four isolates, namely 1, 2, 3, and 8 had chitinase activity with chitinolytic indexes were 1.7; 1.5; 1.4, and 1.3, respectively. Six isolates, namely 1, 2, 3, 4, 5, and 6 had protease activity with proteolytic indexes were 1.4; 1.8; 3.1; 1.3; 1.8; and 2.5, respectively.Keywords: Bacteria; Chitinolytic; Proteolytic; Symbiont; SpongeAbstrakEnzim menempati posisi penting dalam bidang teknologi dan industri. Enzim yang banyak digunakan dalam bidang industri adalah enzim hidrolase. Enzim dapat diisolasi dari berbagai jenis mikroorganisme seperti bakteri. Bakteri yang berasosiasi dengan spons merupakan sumber enzim hidrolitik ekstraseluler yang sangat baik karena permukaan dan ruang internal spons lebih kaya nutrisi. Penelitian ini bertujuan untuk mengisolasi dan menguji aktivitas kitinase dan protease bakteri simbion spons Dragmacidon sp dari Teluk Manado. Bakteri simbion spons ditumbuhkan dalam media Zobell 1226 E pada pengenceran 10-4. Isolasi bakteri dilaksanakan berdasakan karakteristik morfologi. Aktivitas kitinase dan protease dilaksanakan dengan menumbuhkan setiap isolat bakteri dalam media kitin dan protein pada suhu 36oC selama 48 jam. Aktivitas kitinase dan protease ditandai dengan terbentuknya zona bening di sekitar koloni bakteri yang mana untuk kitinase diamati setelah diberi larutan lugol. Berdasarkan penelitian ini, 8 isolat bakeri simbion spon Dragmacidon sp dari Teluk Manado, Sulawesi Utara berhasil diisolasi berdasarkan karakteristik morfologi. Isolat bakteri umumnya berwarna putih dengan bentuk ireguller. Empat isolat yakni 1, 2, 3, dan 8 memiliki aktivitas kitinase dan enam isolat yakni 1, 2, 3, 4, 5, dan 6 yang memiliki aktivitas protease. Indeks kitinolitik dari masing-masing keempat isolat bakteri secara berturut turut adalah 1,7; 1,5; 1,4; dan 1,3 dengan kategori bernilai rendah dan indeks proteolitik adalah 1,4; 1,8; 3,1; 1,3; 1,8; dan 2,5 dengan kategori bernilai rendah sampai tinggi.Kata kunci: Bakteri; Kitinolitik; Proteolitik; Simbion; Spons
Analysis Of Types Of Carotenoid Pigments In Crab Sesarmops sp From Manado By Coast Adrian, Melinda Margareta; Paransa, Darus S. J; Paulus, James J. H; Kawung, Nickson J.; Bara, Robert A.; Kepel, Rene Ch.
Jurnal Ilmiah Platax Vol. 9 No. 2 (2021): ISSUE JULY-DECEMBER 2021
Publisher : Sam Ratulangi University

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.35800/jip.9.2.2021.35127

Abstract

Carotenoid pigments are a group of pigments that are yellow, orange, and red-orange in color. Pigments are natural dyes found in plants and animals, extracts of carotenoid pigments can be separated by chromatographic methods where the common chromatographic methods in determining the type of pigment are Column Chromatography (CC) and Thin Layer Chromatography (TLC). The purpose of this study was to determine the types of pigments contained in the carapace extract of the male crab Sesarmops sp. The Sesarmops sp crab has a brown dorsal carapace with blackish-brown leg spots known as mangrove crabs. Crabs in the Sesarmidae family have a carapace formation and wide legs, do not have swimming legs, and have a pair of claws that are faded purple, the presence of these colors can be identified as containing carotenoid pigments. The results of this study obtained the content of carotenoid pigment 25.2 g/gr and the value of the concentration of carotenoid pigment in the male crab extract Sesarmops sp 10.99 g. The results of the separation of the total pigment extract using column chromatography obtained the types of pigments -carotene, Ekinenon, Zeaxanthin, and Astaxanthin. Keywords: Column Chromatography (CC); Carotenoids; Sesarmops sp                                                                       AbstrakPigmen karotenoid adalah sekelompok pigmen yang berwarna kuning, oranye dan merah oranye. Pigmen adalah zat warna alami yang terdapat pada tumbuhan  dan hewan, ekstrak pigmen karotenoid dapat dipisahkan dengan metode kromatografi yang dimana metode kromatografi umum dalam menentukan jenis pigmen adalah Kromatografi Kolom (KK) dan Kromatografi Lapis Tipis (KLT). Tujuan dari penelitian ini untuk mengetahui jenis-jenis pigmen apa saja yang terdapar pada ekstrak karapas kepiting jantan Sesarmops sp. Kepiting Sesarmops sp memiliki karapas dorsal bewarna coklat dengan bintik kaki coklat kehitaman yang dikenal sebagai kepiting mangrove. Kepiting dalam keluarga sesarmidae memiliki bentukan karapas dan kaki yang lebar tidak memiliki kaki renang serta memiliki sepasang capit berwarna ungu pudar, adanya warna tersebut kepiting dapat diidentikasikan mengandung jenis pigmen karotenoid.  Dari hasil penelitian ini mendapatkan kandungan pigmen karotenoid 25,2 dan nilai konsentrasi pigmen karotenoid pada ekstrak kepiting jantan Sesarmops sp 10,99 . Hasil pemisahan dari ekstrak pigmen total menggunakan kromatografi kolom didapatkan jenis pigmen β-karoten, Ekinenon, Zeaxantin dan Astaxantin
Antibacterial Activity of Stylissa carteri Sponge Extract from Manado Bay, North Sulawesi Palungan, Irpan; Bara, Robert Antonius; Mangindaan, Remy Emile Petrus; Kemer, Kurniati; Wullur, Stenly; Rembet, Unstain N. W. J.
Jurnal Ilmiah Platax Vol. 10 No. 1 (2022): ISSUE JANUARY-JUNE 2022
Publisher : Sam Ratulangi University

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.35800/jip.v10i1.36020

Abstract

Marine sponges contain secondary metabolites with unique chemical structures and very interesting pharmacological activities, such as antibacterial, anticancer, antiviral and others to be developed as candidate drugs. The presence of bioactive metabolites in sessile nature such as sponges also reflects the ecological adaptation formed during a long evolutionary process as a defense mechanism of this organism with its environment in the form of resistance to predation, competition and infection factors against pathogenic bacteria, so this study aims to examine the antibacterial activity of extracts and fractions of Stylissa carteri sponge fractions as well as testing the Minimum Inhibitory Concentration (MIC) and Minimum Bactericidal Concentration (MBC) values. The method used in testing the antibacterial activity is the agar diffusion method (Disc Diffusion Kirby Bauer Method). The presence of antibacterial activity was indicated by the formation of a clear zone around the paper disc after incubation for 24 hours. The results showed that antibacterial activity of S. carteri sponge extract tested on Bacillus megaterium DSM32T bacteria revealed to be the strongest inhibition zone of 21 mm. Further testing on the extract fraction of S. carteri showed that the semipolar fractions showed strong activity against the B. megaterium while the polar fraction was categorized as moderate action, the non-polar fraction showed no activity against the bacteria. The determination of the MIC and MBC values was obtained at 500 ppm and 1000 ppm respectively.
Morphology and Morfometric Study of Crabs Species Caught on the Coast of Mokupa, Minahasa District, North Sulawesi Pelafu, Widya; Paransa, Darus Saadah J.; Mantiri, Desy M. H; Kemer, Kurniati; Bara, Robert A.; Batarogoa, Nego E.
Jurnal Ilmiah Platax Vol. 10 No. 2 (2022): ISSUE JULY-DECEMBER 2022
Publisher : Sam Ratulangi University

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.35800/jip.v10i2.42283

Abstract

Crabs are organisms that have been found moving actively in tidal areas. One is in the coastal area of Mokupa, Whare the study boasts a great diversity of poorly informed crabs. this study aims to identify the morphology and diversity of crab species during the full moon and late nephew phase. The location for sampling is on the coast of Mokupa Beach, Minahasa Regency, North Sulawesi.  This research was conducted using the roaming method and captured directly at night at the lowest low tide based on the phases of the full moon and late nephew. Furthermore, the collected crabs were identified by morphology, body length, body color, carapace shape, pair of claws, walking legs, and abdomen, and morphometric calculations were performed. Based on the morphological identification found, namely: Thalamita crenata, Geograpsus crinipes, Selatium brocki, and Sesarmops impressus.Keywords: Coastal beaches, Morphology, Crab type. AbstrakKepiting merupakan organisme yang ditemukan bergerak aktif di daerah pasang surut. Salah satunya di daerah pesisir Pantai Mokupa, lokasi ini memiliki banyak keanekaragaan jenis kepiting yang belum terinfomasi dengan baik. Penelitian ini bertujuan mengidentifikasi morfologi dan keanekaragaman jenis kepiting saat fase bulan purnama dan perbani akhir. Lokasi penangkapan sampel di pesisir Pantai Mokupa, Kabupaten Minahasa.Sulawesi Utara, dilakukan mengunakan metode jelajah dan ditangkap langsung pada ­waktu malam hari saat surut terendah berdasarkan fase bulan purnama dan perbani akhir. Selanjutnya, kepiting yang yang terkumpul diidentifikasi morfologi diamati ukuran panjang tubuh, warna tubuh, bentuk karapas, sepasang capit, kaki jalan dan abdomen, serta dilakukan perhitungan morfometrik. Berdasarkan identifikasi morfologi ditemukan, yaitu: Thalamita crenata, Geograpsus crinipes, Selatium brocki dan Sesarmops impressus. Kata  kunci: Pesisir pantai,  Morfologi, Jenis kepiting.
Estimation of Carbon Stock in Mangrove Communities in Budo Village, Wori District, North Minahasa Regency Ruru, Ricky Andreas; Rumengan, Antonius Petrus; Paransa, Darus Sa'adah Johanis; Paruntu, Carolus Paulus; Bara, Robert Antonius; Rondonuwu, Ari B.
Jurnal Ilmiah Platax Vol. 11 No. 1 (2023): ISSUE JANUARY-JUNE 2023
Publisher : Sam Ratulangi University

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.35800/jip.v11i1.43629

Abstract

Mangrove is an ecosystem consisting of a collection of trees and grows in the area around the coastline, living in conditions influenced by tides, muddy and sandy sediments. Has an important function in the effort to absorb carbon levels through the photosynthesis process which is better than other forest vegetation. The purpose of this study, namely to identify mangrove species, examine vegetation and determine the value of biomass and estimated carbon stock in Budo Village, Wori District, North Minahasa Regency, North Sulawesi. Data collection in this study used the line transect method with the transect position being on the left, middle and right of the mangrove ecosystem of Budo Village which was pulled along 100 meters per transect, then the quadrants were measured 10 m x 10 m using raffia rope to limit each quadrant with an interval of 10 m. m and the distance between transects is about 50 m. Based on the results of the study, found 4 types of mangroves, namely: Rhizophora mucronata, Rhizophora apiculata, Sonneratia alba, and Bruguiera gymnorrhyza. The highest density values and relative density of species were on transect 3 with a value of 900 trees/ha and 86.53% by Rhizophora apiculata. The species frequency and relative species frequency were highest in each transect owned by Rhizophora apiculata with values of 0.8 and 55.66%, respectively. The highest species cover was Rhizophora apiculata with a value of 2.56 cm/m with a relative species cover of 72.21%. The highest important value index was Rhizophora apiculata on transect 3 with a value of 214.31%. The highest biomass content was on transect 3 with a value of 592.27 tons/ha. The highest carbon content is on transect 3 with a value of 55.67 tons/ha.
Comparative Studies Of Residual Water Level In Manado And Melonguane Coastal Area During Tropical Cyclone In 2021 Azani, Audia Azizah; Djamaluddin, Rignolda; Bara, Robert A; Manu, Lusia; Dien, Heffry Veibert; Manembu, Indri Shelovita
Jurnal Ilmiah Platax Vol. 12 No. 1 (2024): ISSUE JANUARY-JUNE 2024
Publisher : Sam Ratulangi University

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.35800/jip.v12i1.53167

Abstract

North Sulawesi Province, directly bordering the Pacific Ocean, is located in an area with the highest level of tropical cyclone (TC) activity in the world. As a result, the province is vulnerable to the impacts caused by cyclones, including storm surges. The increase in water levels due to this event has the potential to cause coastal flooding. Previous studies in Manado have identified that sea level rise can be detected through residual water level (RWL), making studying the characteristics of RWL in North Sulawesi important. This research focuses on Manado and Melonguane, allowing for a comparison of characteristics. The data used includes tropical cyclone data from the China Meteorological Administration (CMA) and tidal data from the Geospatial Information Agency. The Unified Tidal Analysis and Prediction (UTide) method is used to identify RWL. The analysis was carried out by using a t-test to compare data at the two locations. The results showed that RWL at those locations had significant differences with Melonguane having the higher value between them. Generally, the increase in RWL in Melonguane occurs shortly after the cyclone period, while the RWL in Manado maximum increases 86 hours after TC's first occurrence. Keywords: tropical cyclone; residual water level; UTide.
Co-Authors Abdul, Jihan A. Adrian, Melinda Margareta Alfret Luasunaung Angeline Ngantung, Angeline Angmalisang, Ping Astony Antonius R.B. Ola Ari B. Rondonuwu Azani, Audia Azizah Batarogoa, Nego E. Calvyn F. A. Sondak, Calvyn F. A. Carolus Paulus Paruntu Daisy Monica Makapedua Darus S. Paransa Deiske Adeliene Sumilat, Deiske Adeliene Dien, Heffry Veibert Dwilestari . Edward Nangoy, Edward Faidiban, Aqueline N. Feny Mentang Fitje Losung Frengki P. Menggelea Ginting, Elvy Like Grace Debbie Kandou Grace Sanger, Grace Grevo S Gerung Henoch Awaloei Hidayat, Andre Alfian Indri Manembu Jimmy Posangi Joshian N.W. Schaduw Kepel, Rene Ch. Kipimbob, Eflentina Kurniati Kemer Lasut, Astrid Y. Lintang, Rosita AJ Mangindaan, Remy Mangindaan, Remy Emile Petrus Mantiri, Desy M. H Manu, Lusia Mona P. Wowor Nawea, Yohanis Ni Luh G.L. Jayalandri, Ni Luh G.L. Nickson J. Kawung, Nickson J. O. Mona Wowor Opa, Samuel Palungan, Irpan Pangouw, Excel Paransa, Darus Sa'adah Johanis Paransa, Darus Saadah J. Paulus, James J. H Pelafu, Widya Pelle, Wilmy Pemsi M. Wowor, Pemsi M. Putri, Vita A.D Ramadan, Febrian Rignolda Djamaluddin Roike Iwan Montolalu Rompas, Rizald Rumengan, Antonius Petrus Ruru, Ricky Andreas Salaki, Meiske S. Salawati, Abraham Imanuel Sanadi, Troy Sandra Tilaar Sembiring, Sindiy Cloudya Sihombing, Frengky Silvester B Pratasik Stenly Wullur Tansil, Alberta Y.M. Titi L. Faraknimella, Titi L. Undap, Nani I.J Unstain N. W. J. Rembet, Unstain N. W. J. Veibe Warouw Vivi P. Santoso Widya Astuty Lolo, Widya Astuty Wilhelmina Patty Yolanda A. Kasi