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All Journal AQUATIC SCIENCE & MANAGEMENT Media Teknologi Hasil Perikanan Jurnal e-Biomedik JURNAL PESISIR DAN LAUT TROPIS PHARMACON JURNAL ILMIAH PLATAX JURNAL BIOMEDIK JURNAL LPPM BIDANG SAINS DAN TEKNOLOGI Medical Scope Journal (MSJ)
Robert A. Bara
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Agriculture, Biological Sciences & Forestry Biochemistry, Genetics & Molecular Biology Earth & Planetary Sciences Education Environmental Science Health Professions Immunology & microbiology Languange, Linguistic, Communication & Media Medicine & Pharmacology Nursing Public Health

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Journal : JURNAL BIOMEDIK

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Uji Efek Daya Hambat Jamur Endofit Rimpang Jahe (Zingiber officinale Rosc) terhadap Bakteri Staphylococcus aureus dan Escherichia coli Abdul, Jihan A.; Posangi, Jimmy; Wowor, Pemsi M.; Bara, Robert A.
Jurnal Biomedik : JBM Vol 12, No 2 (2020): JURNAL BIOMEDIK : JBM
Publisher : UNIVERSITAS SAM RATULANGI

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.35790/jbm.12.2.2020.29163

Abstract

Abstract: Ginger rhizome contains antimicrobial compounds of phenol group, flavonoids, terpenoids, and aetheric oil. Endophytic fungi are commonly found in ginger plant Zingiber officinale Rosc. which is widely available in Indonesia. There is a symbiosis between these endophytic fungi and its host plants in a form of genetic material transform, thus these fungi could produce the same active compounds as the host does, or at least involved in the host’s biosynthetic pathway. This study was aimed to determine the inhibitory effect of endophytic fungi isolated from ginger (Zingiber officinale Rosc) against clinical isolates Staphylococcus aureus and Escherichia coli. The endophytic fungi were isolated and their activities were screened. Initial screening of 6 isolates showed that only 3 isolates had relatively strong inhibitory effect against the bacteria. Therefore, they were cultured again, and their extracts were tested against the same types of bacteria. The mean diameters of the inhibition zones against S. aureus were, as follows: 18.7 mm (JJR extract), 11 mm (JJA 1.3 extract), and 17.3 mm (JJA 2.1 extract). Moreover, the mean diameters of the inhibition zone against E. coli were, as follows: 18 mm (JJR extract), 17.3 mm (JJA 1.3 extract), and 23.3 mm (JJA 2.1 extract). In conclusion, endophytic fungi isolated from Zingiber officinale Rosc. had inhibitory effect against S. aureus and E. coli. The inhibitory effect against E. coli was stronger than against S. aureus.Keywords: Zingiber officinale Rosc, endophytic fungi, E. coli, S. aureus Abstrak: Rimpang jahe mengandung senyawa antimikroba fenol, flavonoid, terpenoid, dan minyak atsiri. Jamur endofit sering ditemukan pada tumbuhan jahe Zingiber officinale Rosc, yang tersebar di Indonesia. Terdapat simbiosis antara jamur endofit dan tanaman inang dalam bentuk transformasi materi genetik sehingga jamur tersebut dapat menghasilkan senyawa aktif yang sama, atau setidaknya terlibat dalam jalur biosintesis inang. Penelitian ini bertujuan untuk mengetahui daya hambat jamur endofit yang terdapat pada jahe (Zingiber officinale Rosc) terhadap bakteri Staphylococcus aureus dan Escherichia coli. Jamur endofit diisolasi dan diskrining aktivitasnya. Hasil skrining awal dari 6 isolat yang diperoleh memperlihatkan hanya 3 isolat jamur endofit memiliki daya hambat yang relatif kuat terhadap bakteri uji. Ketiga isolat tersebut dikultur kembali dan ekstraknya diuji aktivitas dengan bakteri yang sama. Hasil pengukuran zona hambat 3 isolat jamur ialah sebagai berikut: pada bakteri Staphylococcus aureus didapatkan jamur JJR 18,7 mm, jamur JJA 1.3 11 mm, dan JJA 2.1 17,3 mm; pada bakteri Escherichia coli didapatkan jamur JJR 18 mm, JJA 1.3 17,3 mm, dan JJA 2.1 23,3 mm. Simpulan penelitian ini ialah jamur endofit isolat dari Zingiber officinale Rosc. memiliki aktivitas antibakteri terhadap S. aureus dan E. coli. Daya hambat jamur endofit lebih besar terhadap E. coli dibandingkan S. aureus.Kata kunci: Zingiber officinale Rosc, jamur endofitik, E. coli, S. aureus
Co-Authors Abdul, Jihan A. Adrian, Melinda Margareta Alfret Luasunaung Angeline Ngantung, Angeline Angmalisang, Ping Antonius R.B. Ola Ari B. Rondonuwu Azani, Audia Azizah Batarogoa, Nego E. Calvyn F. A. Sondak, Calvyn F. A. Carolus Paulus Paruntu Daisy Monica Makapedua Darus S. Paransa Deiske Adeliene Sumilat, Deiske Adeliene Dien, Heffry Veibert Dwilestari . Edward Nangoy, Edward Faidiban, Aqueline N. Feny Mentang Fitje Losung Frengki P. Menggelea Ginting, Elvy Like Grace Debbie Kandou Grace Sanger, Grace Grevo S Gerung Henoch Awaloei Indri Manembu Jimmy Posangi Joshian N.W. Schaduw Kepel, Rene Ch. Kipimbob, Eflentina Kurniati Kemer Lasut, Astrid Y. Lintang, Rosita AJ Mangindaan, Remy Mangindaan, Remy Emile Petrus Mantiri, Desy M. H Manu, Lusia Mona P. Wowor Nawea, Yohanis Ni Luh G.L. Jayalandri, Ni Luh G.L. Nickson J. Kawung, Nickson J. O. Mona Wowor Opa, Samuel Palungan, Irpan Pangouw, Excel Paransa, Darus Sa'adah Johanis Paransa, Darus Saadah J. Paulus, James J. H Pelafu, Widya Pelle, Wilmy Pemsi M. Wowor, Pemsi M. Pratasik, Silvester Putri, Vita A.D Ramadan, Febrian Rampengan, Royke Rignolda Djamaluddin Roike Iwan Montolalu Rompas, Rizald Rumengan, Antonius Petrus Ruru, Ricky Andreas Salaki, Meiske S. Salawati, Abraham Imanuel Sanadi, Troy Sandra Tilaar Sembiring, Sindiy Cloudya Sihombing, Frengky Stenly Wullur Tambunan, Citra Tansil, Alberta Y.M. Titi L. Faraknimella, Titi L. Undap, Nani I.J Unstain N. W. J. Rembet, Unstain N. W. J. Veibe Warouw Vivi P. Santoso Widya Astuty Lolo, Widya Astuty Wilhelmina Patty Yolanda A. Kasi