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All Journal International Journal of Public Health Science (IJPHS) BALI MEDICAL JOURNAL (BMJ) Medicina E-Jurnal Medika Udayana Jurnal Veteriner Jurnal Pendidikan Biologi Undiksha Medical Journal of Indonesia Indonesian Journal of Tropical and Infectious Disease Bali Journal of Anesthesiology ISM (Intisari Sains Medis) : Jurnal Kedokteran Jurnal Kedokteran: Media Informasi Ilmu Kedokteran dan Kesehatan Journal of Health Sciences and Medicine Bali Dental Journal Journal of Clinical Microbiology and Infectious Diseases Jurnal Widya Medika
Ni Nyoman Sri Budayanti
Departemen Mikrobiologi Klinik Fakultas Kedokteran Universitas Udayana
Agriculture, Biological Sciences & Forestry Biochemistry, Genetics & Molecular Biology Dentistry Earth & Planetary Sciences Education Health Professions Immunology & microbiology Medicine & Pharmacology Public Health Veterinary Other

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Journal : BALI MEDICAL JOURNAL (BMJ)

 1 
IMMUNE RESPONSE AND COST ANALYSIS OF INTRADERMAL RABIES VACCINATION FOR POST-EXPOSURE PROPHYLAXIS REGIMEN IN HUMAN Budayanti, N. S.; Susilawathi, N. M.; Darwinata, A. E.; Dwija, I. B. P.; Fatmawati, N. D.; Wirasandhi, K.; Subrata, K.; Susilarini, N. K.; Wignall, F. S.; Sudewi, A. A. R.; Mahardika, and G. N. K.
BALI MEDICAL JOURNAL Vol 3 No 1 (2014)
Publisher : BALI MEDICAL JOURNAL

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Background The outbreak of rabies in human in Bali-Indonesia is causing an extraordinary pressure for the government in providing adequate doses of anti-rabies vaccine for post-exposure prophylaxis (PEP). Here, we directly compare the immune response and benefit of the intradermal (ID) protocol for rabies vaccine delivery with the intramuscular (IM) route. Methods: Sixty health workers who were willing to participate in this study have been randomly selected and grouped into ID, IM, and control groups, each with 20 volunteers. The Thai Red Cross ID- and Zangreb IM-protocols have been applied to the respective group. The sera of the volunteers were collected at day 0, week 1, week 3, week 4, month 3, month 6, month 9, and month 12 after the first vaccination. Anti-rabies virus IgG was detected using PlateliaTM Rabies II Kit (Bio-Rad). Results: Anti-rabies IgG could be detected in the ID-group at one week. The ID-vaccine delivery induced a slightly higher maximum antibody titer compared to IM, though not statistically significant (p>0.05). ID vaccination caused less adverse reactions and produces longer lasting protective immune response. Cost minimization analysis (CMA) on the provincial and national PEP data in 2009-2011 shows that the ID-delivery will reduce the total cost for a completed regimen by USD 28.5, and would have saved the Indonesian government budget approximately USD 3.6 and 4.3 million for complete regimens in Bali and Indonesia, respectively. Conclusion: The ID administration of anti-rabies vaccine induces a similar immune response compared to that of intramuscular injection. It also produces longer lasting protective immune response. It offers additional advantages of potential net cost savings as well as decreasing the pressure on vaccine availability due to the high number of dog bite cases.
GROUP-B STREPTOCOCCUS IN PREGNANT WOMEN: Prevalence of Colonization and Sensitivity Pattern in Denpasar during June 2007?May 2008 Sri-Budayanti,, N; Hariyasa-Sanjaya, and, N
BALI MEDICAL JOURNAL Volume 2 Number 1, January-April 2013
Publisher : BALI MEDICAL JOURNAL

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Objective: Group-B Streptococci (GBS) are Gram-positive cocci that are the most common cause of early onset neonatal sepsis. The mortality rate of early onset neonatal sepsis has been reported up to 50%. One of the major risks of early onset neonatal sepsis is GBS colonization in birth canal of pregnant women that can infect the baby during process of vaginal delivery. Antibiotic chemoprophylaxis for pregnant women that is colonized by GBS can reduce the risk of early onset neonatal sepsis. The detection of GBS colonization needs Todd Hewitt (TH) enrichment medium to reduce false negative result. Until now, there is no report about either prevalence of colonization or sensitivity pattern of Group B Streptococcus among pregnant women in Denpasar. The aims of this research were to determine the prevalence of GBS colonization and sensitivity pattern of GBS among pregnant women with Todd Hewitt enrichment medium. Method: This research was a descriptive cross-sectional study. Vaginal swab specimens from 35-37 weeks gestation pregnant women were collected and 32 samples that met the inclusion criteria were cultured on Blood agar (BA) plates, Chromagar (CA) plates, and Todd Hewitt (TH) broth. The GBS colonization that grew in culture medium was followed by antibiotic sensitivity test. Results: In the present study, we found that the prevalence of GBS colonization in pregnant women detected with culture method using BA and CA without TH broth was 9.4%, whereas the prevalence with culture method using BA and CA enriched by TH broth was 31.3%. Moreover, GBS showed resistance to penicillin, erythromycin, and cefazolin. It is indicated that TH enrichment medium seems to be promising as a screening method for GBS colonization in pregnant women in Bali. Conclusion: There was an enrichment detection of GBS prevalence colonization in pregnant women detected the swab with culture method using BA and CA enriched by TH compare to BA and CA without TH broth. Moreover, GBS showed resistance to penicillin, erythromycin, and cefazolin. It is indicated that TH enrichment medium seems to be promising as a screening method for GBS colonization in pregnant women in Bali.
Application Anti Microbial Activity Test and Direct Inoculation of Urinary Specimen Test to Increase the Quality of Results and Decrease the Production Cost in Clinical Microbiology Laboratory, Sanglah General Hospital Hospital, Bali-Indonesia Sri-Budayanti, N.; Sukrama, I. D. M.; Aditarini, M.; Sukardika, I. K.; Suata, I.K.
BALI MEDICAL JOURNAL Volume 1, Number 2, May-August 2012
Publisher : BALI MEDICAL JOURNAL

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Objective: Urinary tract infection (UTI) is the most common bacterial infection in general practice and in hospitals. Fast and accurate urine culture and sensitivity test are needed for adequate therapy. Anti Microbial Activity test (AMA test) that is used to detect the presence of antibiotics in urine specimens is not commonly used in clinical microbiology laboratories. Some laboratories are still using indirect inoculation technique using enriched media before inoculating onto the agar media. The aim of this research is to compare results of urinary examination of direct inoculation technique with AMA test with indirect inoculation technique without AMA test. Methods: A number of 210 urine specimens were collected in Clinical Microbiology Laboratory at Sanglah General Hospital within a time period between 16 June until 16 July 2009. Results: Antibiotics were detected in 40% of the urinary specimens; whereas 48.1% showed no evidence of UTI, that is negative AMA test and sterile urinary culture or colony growth < 105 CFU/ml. Only 11.9% of the specimens indicates urinary tract infections. The examination can be completed within 2-3 days which is shorter than indirect inoculation test which require 5-7 days. Direct inoculation technique can reduce the cost of production three-fold the costs require for an indirect inoculation test. Conclusions: Application of AMA test and direct inoculation technique can give results more rapidly, reliable and useful for clinicians. This also decrease the laboratory’s cost of production.
Co-Authors A. Soebandrio A. Widayati Adhiputra, I Ketut Agus Indra Adinda Putra Pradhana Agus Eka Darwinata Agus Roy Rusly Hariantana Hamid Agus Simahendra Agustinus I Wayan Harimawan Ajeng Diantini Amin, Yusuf Sidang Anggita Dewi Arikandini, Dewa Ayu Putu Aisaka Rajeshi Aryana, I Gusti Putu Suka Bryan Setiawan Candra, I Kadek Bayu Adhi Chandra, Katherine Silvania Christopher Ryalino D A Indah Gitaswari Dave Gerald Oenarta Dea Antariksa Dewa Ayu Agung Anggita Ningrat Dwija, Ida Bagus Nyoman Putra Dyah Pradnyaparmita Duarsa Eriata, Anselmus Helbert Erly Sintya Erly Sintya F. S. Wignall Fedik Abdul Ratam Gusti Ayu Dianti Violentina Hearty Indah Oktavian Hendrawan, Gresya I Dewa Agung Gede Meisha Dhanam I Dewa Ayu Made Dian Lestari I Dewa Made Sukrama I Gede Gita Sastrawan I Gede Pradnya Wisnu Murthi I Gusti Agung Indah Pradnyani R.S I Gusti Ayu Dewi Ratnayanti I Gusti Kamasan Arijana I Gusti Ngurah Kade Mahardika I Gusti Ngurah Mayun I Gusti Nyoman Sri Wiryawan I Made Ady Wirawan I Made Sutha Saskara I Nyoman Hariyasa Sanjaya I Nyoman Wande I Putu Bayu Mayura I Putu Bayu Mayura, I Putu Bayu I Wayan Adi Pranata I Wayan Rivandi Pradiyadnya Mardana I Wayan Sugiritama I Wayan Suranadi I. B. P. Dwija I. K. Sukardika I.K. Suata Ida Ayu Andhira Dewi Suarisavitra Ida Ayu Putu Putri Andari Ida Bagus Gede Adiguna Wibawa Ida Sri Iswari Indramawan Setyojatmiko Indraningrat, Anak Agung Gede K. Subrata K. Wirasandhi Kadek Anggie Wigundwipayana Kadek Dede Frisky Wiyanjana Kadek Karang Agustina Komang Januartha Putra Pinatih Krishna Murprayana Lameng, Imaculata Sonia Vidaryo M. A.C.M. Nusatya Made Agus Hendrayana Made Widianantara Made Wiryana Marco Reeiner N. K. Niti Susila, N. K. N. K. Susilarini Ni Kadek Seri Mahayanti Ni Luh Made Mirah Rahayu Ni Luh Putu Harta Wedari Ni Luh Putu Harta Wedari Ni Luh Putu Harta Wedari, Ni Luh Putu Harta Ni Luh Ranthi Kurniawathi Ni Made Adi Tarini Ni Made Linawati Ni Made Mertaniasih Ni Made Susilawathi Ni Nengah Dwi Fatmawati Ni Nengah Dwi Fatmawati Nyoman Indra Karunia Putri Nyoman Yenny K, Nyoman Pande Putu Januraga Prilandari, Luh Inta Prisela Zharaswati Prisillia Brigitta Putra, I Dw. Gd. Bayu Artha Pratama Putra, Made Dwi Ambara Putu Arya Suryanditha Putu Dian Pratita Lestari Putu Eka Arimbawa Raka-Sudewi A. A. Rastuti, Made Rina Rustawan, I Nengah Tony Saranova, Hilda Sari Kusumadewi Sathya Deva, I Dewa Gde Setiabudy, Marta Shita Diwyani Sudarsa Tjokorda Gde Agung Senapathi V. Wiwing Violentina, Gusti Ayu Dianti Wahyu Hidayati Wibisana, I Dewa Nyoman Adi Ningrat Widowati, I Gusti Ayu Rai - Wijaya, Carolin Winatha, I Gde Pangestu Putrama Winaya, Made Illene Wira Guna, I Gede Bhima Yanti, Ni Komang Semara Yuntari, Gusti Ayu Krisma