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All Journal HAYATI Journal of Biosciences Jurnal Agronomi Indonesia (Indonesian Journal of Agronomy) Jurnal Ilmu Pertanian Indonesia Jurnal Teknologi Dan Industri Pangan Journal of Tropical Life Science : International Journal of Theoretical, Experimental, and Applied Life Sciences Jurnal Veteriner Microbiology Indonesia BIOTROPIA - The Southeast Asian Journal of Tropical Biology Jurnal Pengelolaan Sumberdaya Alam dan Lingkungan (Journal of Natural Resources and Environmental Management) Jurnal Sumberdaya Hayati JURNAL BIOLOGI INDONESIA ANNALES BOGORIENSES Biosaintifika: Journal of Biology & Biology Education Menara Perkebunan Floribunda Jurnal Ilmiah Sains Annales Bogorienses
Nampiah Sukarno
Department Of Biology, Faculty Of Mathematics And Natural Sciences, IPB University, Bogor, Indonesia
Agriculture, Biological Sciences & Forestry Biochemistry, Genetics & Molecular Biology Chemical Engineering, Chemistry & Bioengineering Chemistry Computer Science & IT Earth & Planetary Sciences Economics, Econometrics & Finance Environmental Science Immunology & microbiology Industrial & Manufacturing Engineering Mathematics Medicine & Pharmacology Physics Veterinary

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THE OCCURENCE OF CAUSAL FUNGUS OF BANANA LEAF SPECKLE, ON PSEUDOSTEM OF MUSA X PARADISIACA IN INDONESIA Desi Maria Panjaitan; Nampiah Sukarno; Mien Ahmad Rifai
Floribunda Vol. 5 No. 5 (2016)
Publisher : PTTI

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (86.112 KB) | DOI: 10.32556/floribunda.v5i5.2016.162

Abstract

The banana leaf parasite hyphomycete Ramichloridium musae is recorded for the first time on dried banana pseudostem from Java.
Cellulolytic Yeast Isolated From Raja Ampat Indonesia Atit Kanti; Nampiah Sukarno; Endang Sukara; Latifah K Darusman
ANNALES BOGORIENSES Vol 16, No 1 (2012): Annales Bogorienses
Publisher : Research Center for Biotechnology - Indonesian Institute of Sciences (LIPI)

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (600.042 KB) | DOI: 10.14203/ann.bogor.2012.v16.n1.27-34

Abstract

The objective of this study was to select and characterize three yeast isolates originating from soil of  Raja Ampat region of Papua, Indonesia for its potential to produce cellulase . Selection and characterization of cellulolytic yeast was carried out by measuring cellulolytic Index (IS) with congo red method and measurement of Carboxy Methyl Cellulase (CMC-ase) activity through determination of reducing sugar with dinitrosalycilic methods. Cellulolytic Index (IS) of the isolates  Sporobolomyces poonsookiae Y08RA07,  Rhodosporidium paludigenum Y08RA29 and Cryptococcus flavescens Y08RA33were 1.40, 2.60 and 1.66 respectively. CMC-ase produced optimum at pH 8 at 37ºC by isolate Y08RA07, whereas for Y08RA29 andY08RA33 were at pH 6, at 28ºC. Paper waste was good substrate for cellulase enzyme production by isolate Y08RA07, while for two other isolates the best substrate was CMC. Isolate Y08RA29 having highest cellulase activities when grown in CMC, while isolates Y08RA07 and Y08RA33 achieved highest enzyme activity when grown in bamboo leaf.   Key words: Cellulolytic yeast, Raja Ampat, waste paper, bamboo leaf
Endophytic Fungi Isolated from the Mangrove Species Rhizophora apiculata and Their Efficacy as Herbicides Za’aziza Ridha Julia; Nampiah Sukarno; Sintho Wahyuning Ardie; Irmanida Batubara; Soekisman Tjitrosoedirdjo; Mashuri Waite
HAYATI Journal of Biosciences Vol. 29 No. 5 (2022): September 2022
Publisher : Bogor Agricultural University, Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.4308/hjb.29.5.605-620

Abstract

Endophytic fungi play an important role on mangrove growth and development, however research on the endophytic fungi of the mangrove Rhizophora apiculata is limited. The endophytic fungi produce diverse bioactive compounds involved in the mangrove’s adaptation to varied biotic and abiotic stresses and could have applied uses in agriculture or medicine. The purpose of this research was to isolate endophytic fungi of Rhizophora apiculata mangrove and to study their activity as herbicides on the weed Gomphrena globosa. The fungi were isolated using the surface sterilization method and identified based on morphological characteristics and molecular characteristics using ITS regions of rDNA. Herbicidal activity of the fungal filtrates extracted by ethyl acetate were tested on seed germination and seedling growth of G. globosa. Five fungal isolates were obtained, namely Penicillium citrinum, Diaporthe eucalyptorum, Diaporthe musigena, Colletotrichum queenslandicum, and Diaporthe tectonae. All isolates were able to grow on PDA medium containing 0, 25, 50, 75, and 100 (% v/v) seawater concentrations, but the growth rate varied by species and seawater concentration. In general, all five isolates showed herbicidal activity by delaying seed germination and reducing shoot and root growth. P. citrinum showed the highest herbicidal activity compared to the other isolates. Analysis using Gas Chromatography-Mass Spectrometry of the crude extract of P. citrinum filtrate identified 7 main compounds: 3-Methoxy-2-methyl-cyclohex-2-enone, Cyclohexane-carbohexaldehyde, 6-methyl-3-(1-methylethyl)-2-oxo, Cyclopropane carboxylic acid, 1-(2-propenyl)-1,1-dimethylethyl ester, 2-Hydroxy-4-isopropyl-7-methoxytropone, Beta-Asarone, Oxane, 4-(2-amino-1-hydroxyethyl)-4-(3,4-dimethoxyphenyl)-, and Diisooctyl-phthalate. These compounds should be studied further to determine which ones are responsible for the herbicidal activity.
PERTUMBUHAN TANAMAN JARAK PAGAR (Jatropha curcas L.) PADA LAHAN PASCA TAMBANG TIMAH DI BANGKA YANG DIBERI PUPUK ORGANIK Sukmarayu P Gedoan; Alex Hartana; Hamim Hamim; Utut Widyastuti; Nampiah Sukarno
JURNAL ILMIAH SAINS Volume 11 Nomor 2, Oktober 2011
Publisher : Sam Ratulangi University

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (199.582 KB) | DOI: 10.35799/jis.11.2.2011.205

Abstract

PERTUMBUHAN TANAMAN JARAK  PAGAR (Jatropha curcas L.) PADA LAHAN PASCA TAMBANG TIMAH DI BANGKA YANG DIBERI PUPUK ORGANIK Sukmarayu P. Gedoan1), Alex Hartana2), Hamim2), Utut Widyastuti2) dan Nampiah Sukarno2) 1)Program Studi Biologi, FMIPA Universitas Negeri Manado di Tondano; 2)Departemen Biologi, FMIPA, Institut Pertanian Bogor, Bogor 16680   ABSTRAK Kegiatan penambangan timah menyebabkan perubahan karakteristik fisika dan kimia tanah sehingga menjadi tidak sesuai untuk pertumbuhan tanaman.  Tujuan penelitian ini untuk mempelajari pertumbuhan 7 aksesi jarak pagar yang ditanam pada lahan pasca tambang timah yang diberi kompos dan kotoran sapi.  Penelitian ini dilakukan di TSS 133, Kelurahan Sinar Baru, Kabupaten Bangka, Provinsi Bangka Belitung.  Penelitian lapangan dilaksanakan pada Bulan Mei 2007 sampai dengan Bulan Desember  2008.  Percobaan faktorial ini dirancang dalam Rancangan Petak Terbagi dengan tiga ulangan.  Petak utama adalah 7 aksesi jarak pagar yang terdiri atas: aksesi Madiun, Ponorogo, Jember, Dompu, Lampung, Bengkulu,  dan Sukabumi, sedangkan  anak petak berupa tanah tanpa pemberian kompos dan pupuk kandang (kontrol), kompos trubus 4 kg/lubang ditambah 4 kg tanah bagian atas  dan  kotoran sapi 4 kg/lubang ditambah 4 kg tanah bagian atas. Hasil penelitian menunjukkan bahwa pertumbuhan awal yang paling baik untuk tanah bekas tambang timah adalah pada aksesi Jember yang ditunjukkan oleh diameter batang, berat kering tanaman, berat kering tajuk dan berat kering akar.  Sedangkan tinggi tanaman tertinggi diamati pada aksesi Madiun.  Penambahan kotoran sapi dapat meningkatkan produksi biji dan kandungan minyak.  Produksi biji tertinggi diperoleh pada aksesi Bengkulu dan kandungan minyak tertinggi diperoleh pada aksesi Dompu. Kata kunci: aksesi, Jatropha curcas, kompos, kotoran sapi   THE GROWTH OF CASTOR OIL PLANT (Jatropha curcas L.) ON THE POST-TIN-MINING LAND IN BANGKA PROVIDED WITH ORGANIC FERTILIZER ABSTRACT The activity of tin mining changes the physical and chemical characteritics of soil, so that the soil isnot suitable for plant growth.  The objective of this research was to evaluate the growth of some accession of Jatropha curcas that we planted on post-tin-mining land provided with compost and cow feces.   This research was conducted in TS 133, Sinar Baru Village, District of Bangka, Province of Bangka Belitung.  This field research was carried out in May 2007 to December 2008.  Factorial experiment was designed as split plot with three replications.  The main plot was 7 accessions, i.e. Madiun, Ponorogo, Jember, Dompu, Lampung, Bengkulu, and  Sukabumi.  The subplot waskonds of organic fertilizer, i.e. top soil without addition of compost and cow fecer (control), trubus compost  (4 kg/hole) added with top soil (4 kg/hole), and cow feces (4 kg/hole) added with topbsoil (4 kg/hole). The result showed that Jember accession had the best early growth based on the observation of stem diameter, plant dry weight, and root dry weight.  The largest plant height was observed in Madiun accession.  The higest seed production was observed in Bengkulu accession and Dompu accession had the higest oil content.Keywords: accession, Jatropha curcas, , compost, cow feces
Growth and Development of Tristaniopsis merguensis Seedling Inoculated by Natural Ectomycorrhiza Triadiati Triadiati; Anastastia Raditya Hidayanti; Nampiah Sukarno
Journal of Tropical Life Science Vol. 10 No. 2 (2020)
Publisher : Journal of Tropical Life Science

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.11594/jtls.10.02.01

Abstract

Tristaniopsis merguensis (pelawan tree) is a potential plant, however, it has not cultivated and conserved optimally due to slow growth and lack of information about the cultivation. The existence of T. merguensis in the forest is important because pelawan fungi grow under the tree, particularly in the above of the roots. Information about how to grow the T. merguensis seedling is very limited; therefore, research in growth and development of T. merguensis seedling was important. The aims of this study were to determine the appropriate planting medium for T. merguensis seed germination and to stimulate growth and development of T. merguensis seedling by using its fungi. Status of T. merguensis fungi was studied by analyzing root morphology. Fungi isolation was carried out from colonized root and fungi fruit body. The stimulated the growth of T. merguensis seedling was done by using fungi isolated from colonization root in laboratory scale, as well as treated at a different phosphate concentration. The result showed that T. merguensis was associated with ectomycorrhizal fungi. Planting medium consists of sawdust and T. merguensis fine root resulted the highest percentage of germination. Seedlings were treated with ectomycorrhizal fungi grew better than without ectomycorrhizal fungi. In addition, seedlings treatment with ectomycorrhizal fungi and phosphate 25% showed the highest growth rate. 
Isolasi Cendawan Mikoriza Arbuskula dari Rizosfer Tanaman Berkayu Asal Pulau Bangka dan Karakteristik Struktur Kultur Mikorizanya: Isolation of Arbuscular Mycorrhizal Fungi from Rhizosphere of Bangka Island Woody Plants and their Mycorrhizal Structure Culture Characteristics Nampiah Sukarno; Cici Rahmawati; Sri Listiyowati; Wendi Nurul Fadillah; Yanti Novera
Jurnal Sumberdaya Hayati Vol. 9 No. 2 (2023)
Publisher : Departemen Biologi, Institut Pertanian Bogor

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.29244/jsdh.9.2.39-48

Abstract

Arbuscular Mycorrhizal Fungi (AMF) associate with a variety of plants including forest trees. Research on AMF in forestry in Indonesia is limited, especially on woody plants grown in Bangka Island. Therefore, the aim of this research was to isolate and identify AMF associated with woody plants from Bangka Island grown in the post-mining soil in greenhouse for 7 years. The Soil samples derived from 8 pots of the 7 years old woody plants were used as spore sources. Spore extraction from the soil used the wet sieving and decanting method. Fungal identification was carried out based on morphological characteristics, and fungal isolation used pot culture with Pueraria javanica as a host plant. Fungal structures within the root were analyzed after root staining with trypan blue. There were 18 spore morphotypes observed which belong to 5 types of Acaulospora and 13 types of Glomus. Seven single spore pot cultures were successfully isolated from species of Acaulospora sp.1, Acaulospora sp.4, Acaulospora sp.5, Glomus sp.2, Glomus sp.3, Glomus sp.6, and Glomus sp.11. AM fungal colonization structures observed within the root were Paris type arbuscules, internal hyphae, and vesicles. The AMF cultures obtained could be used as biofertilizer for woody plant seedlings production for post mining reclamation activities.
Trichoderma hamatum derived from coffee plant ( Coffea canephora ) rhizosphere inhibit Candida albicans Growth Yuyun Nisaul Khairillah; Nampiah Sukarno; Irmanida Batubara
Biosaintifika: Journal of Biology & Biology Education Vol 13, No 3 (2021): December 2021
Publisher : Department of Biology, Faculty of Mathematics and Sciences, Semarang State University . Ro

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.15294/biosaintifika.v13i3.31132

Abstract

The Trichoderma hamatum produces various secondary metabolites that can be used as Candida albicans.  This research aimed to isolate T. hamatum from the coffee (Coffea canephora) rhizosphere and analyze the fungal compounds to control the pathogenic fungus C. albicans. T. hamatum was isolated using the dilution method, and the fungal identification was used, combining morphological and molecular characteristics of ITS rDNA. The potency of T. hamatum as C. albicans was determined by antagonist test using the double-layer method, while for culture filtrate, ethyl acetate and n-hexane filtrate extracts were carried out by the agar diffusion method. The compounds in the most active extract were analyzed by Gas Chromatography-Mass Spectrometry (GC-MS). T. hamatum inhibits C. albicans growth in antagonistic and filtrate with 15.53 mm and 14.40 mm of inhibition zone. It indicated that both culture and fungal filtrate had similar activities on C. albicans. The ethyl acetate extract (minimum inhibitory concentration, MIC of 0.50%) showed more potent against C. abicans than n-hexane extract (MIC of 15.00%). The potential active compound in the ethyl acetate extract would be 9-Octadecenoic acid (Z) methyl ester. The 9-Octadecenoic acid (Z) methyl ester could be used as an alternative candidate to control Candida.
Isolasi dan Identifikasi Cendawan Endofit Akar Anggrek Epifit dan Hemiepifit: Isolation and Identification of Endophytic Fungi in Epiphytic and Hemiepiphytic Orchid Root Michael Michael; Nampiah Sukarno; Sofi Mursidawati; Edhi Sandra; Nadiya Dwi Rahayu
Jurnal Sumberdaya Hayati Vol. 9 No. 4 (2023): 2023
Publisher : Departemen Biologi, Institut Pertanian Bogor

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.29244/jsdh.9.4.152-163

Abstract

Orchids form a symbiotic relationship with endophytic fungi throughout their life cycle, including their seed germination. Research on orchid endophytic fungi in Indonesia is still limited. This study aimed to isolate and identify endophytic fungi of Phalaenopsis amabilis, Dipodium brevilabium, Vanilla planifolia, and Aerides odorata orchids. Endophytic fungi were isolated from surface-sterilized orchid root pelotons. Fungal identification was based on morphological and molecular characteristics using ITS rDNA sequences, followed by BLAST and phylogenetic tree analysis. All orchids studied had peloton structures in root cortex tissues. From the four orchids studied, 22 fungal isolates were obtained. Five fungal isolates were obtained from the P. amabilis orchid, consisting of 1 Penicillium and 4 sterile mycelium isolates. The D. brevilabium had 7 fungal isolates, namely 1 Penicillium and 6 Fusarium isolates. The V. planifolia had 6 Fusarium isolates, whereas the A. odorata had 4 isolates, such as 1 Penicillium, 2 sterile mycelium, and 1 unidentified isolate. The genus Penicillium was found in 3 out of the 4 orchid species studied. One Penicillium isolate was molecularly identified as Penicillium citrinum. Endophytic fungi in P. amabilis were mostly sterile mycelium, while D. brevilabium, V. planifolia, and A. odorata were dominated by Fusarium.
Exploring Indonesian Sponge-Associated Marine Aspergillus hortai: Characterization of Bioactive Compounds with Potential Anti-Escherichia coli Properties Fadillah, Wendi Nurul; Sukarno, Nampiah; Iswantini, Dyah; Rahminiwati, Min; Franco, Christopher MM; Zhang, Wei; Hanif, Novriyandi; Waite, Mashuri
HAYATI Journal of Biosciences Vol. 31 No. 4 (2024): July 2024
Publisher : Bogor Agricultural University, Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.4308/hjb.31.4.712-724

Abstract

Sponge-associated marine fungi are potential source for secondary metabolite compounds. The aim of this research was to investigate sponge-associated marine fungus as secondary metabolite producers against Escherichia coli. The fungus was isolated from Indonesian marine sponge Stylissa sp. and identified as Aspergillus hortai through a combination of morphological and molecular characteristics of ITS DNA and β-tubulin genes. The fungus was tested against E. coli using fungal broth and mycelial extracts. The optimized condition was achieved by fungal broth grown in corn meal broth at 6-days of shaking incubation. Fungal extract was produced using three liters of filtered fungal broth and extracted in ethyl acetate. The antibiotic activity of the extract is vulnerable to 45°C heat and basic or acidic conditions. Therefore, the extraction was done at pH 7 with evaporation at 40°C. The extract shows 7 major bands on TLC with 1 band shows activity against E. coli (Rf 0.81) on bioautogram. The band was observed as a yellow color and turned black in short-wave UV and did not show any fluorescence in long-wave UV. This research shows that sponge-associated marine fungi obtained from Indonesia has the potential as anti E. coli worth to be explored for searching new antibiotics.
Potensi Cendawan Endofit Asal Jahe Merah (Zingiber officinale Roscoe) untuk Mengendalikan Cendawan Patogen Candida albicans In Vitro: Potency of Endophytic Fungi Isolated From Zingiber officinale Roscoe to Control Pathogenic Fungus Candida albicans In Vitro Sari, Mulya; Sukarno, Nampiah; Batubara, Irmanida; Cinta Badia Br Ginting, Rohani
Jurnal Sumberdaya Hayati Vol. 6 No. 1 (2020)
Publisher : Departemen Biologi, Institut Pertanian Bogor

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.29244/jsdh.6.1.26-32

Abstract

Endophytic fungi isolated from red ginger (Zingiber officinale) can inhibit growth of Fusarium oxysporum, but the ability of the fungi to control Candida albicans as human pathogen has not been reported. The aims of this research were to study the mechanism of ten endophytic fungi isolates derived from red ginger to control C. albicans in vitro using dual culture methode and fungal extract, and to determine fungal bioactive chemical groups produced by the fungi. Three out of ten isolates tested, Talaromyces assiutensis JMa 7, T. assiutensis JMbt 3, and Curvularia affinis JMbt 9 inhibited growth of C. albicans with inhibition zones were 4.0 mm, 4.9 mm, and 11.3 mm, respectively. The cultures of the three potential endophytic fungi were extracted by maceration method using 3 solvents i.e ethyl acetate, n-hexane and ethanol. The three isolates were grown in PDB separately for 21 days incubation. At harvest, the culture filtrate was extracted by ethyl acetate and n-hexane, while fungal mycelia were extracted by all the three solvents. Ethyl acetate extracts obtained from culture filtrate of all the three fungal isolates consistently inhibited C. albicans with inhibition zones were 2.0-3.8 mm. For n-hexane extract, however, only Talaromyces assiutensis JMbt 3 that had positive effect with inhibition zone was 2.0 mm. All extracts from mycelia did not have any effects on C. albicans. The ethyl acetate extract of T. assiutensis JMbt 3 was analysed to determine its chemical groups using visible color on thin layer chromatography (TLC). The results showed that the bioactive compounds was terpenoids, and antioxidant.
Co-Authors , Triadiati ADI YULANDI Agustin Wydia Gunawan Alex Hartana ALEX HARTANA Alex Hartana Anastastia Raditya Hidayanti Astri Ariyani Atit Kanti Atit Kanti Cici Rahmawati Cinta Badia Br Ginting, Rohani Dedi Fardiaz Desi Maria Panjaitan Didiek Hadjar Goenadi Dyah Iswantini Edhi Sandra Edi Guhardja Endang Sukara Eris Septiana Fachrian Hasmi Pasaribu Fachriyan Hasmi Pasaribu Fadillah, Wendi Nurul Franco, Christopher MM Hamim Hamim HAPPY WIDIASTUTI Hidayanti, Anastastia Raditya I Made Sudiana I Nyoman Pugeg Aryantha Ibnu Qayim Irmanida Batubara Kartini Kramadibrata Kemala S. Nagur L K Darusman Lady Diana Tetelepta, Lady Diana Latifah K Darusman latifah K Darusman Latifah K. Darusman LATIFAH KOSIM DARUSMAN Latifah Kosim Darusman Latifah Kosim Darusman M. Rafi M. Sukarno Mashuri Waite Mashuri Waite Maulani, Nail Izzatul Mega Putri Amelya Michael Michael Mien Ahmad Rifai Mujahidah, Shofia Mujahidah, Shofia Mulya Sari Nadia T. Hendartina Nadiya Dwi Rahayu Novriyandi Hanif Partomuan Simanjuntak Putra, Sukma Triperdana Rahayu Laelandi Rahayu, Nadiya Dwi Rahminiwati, Min Rida Oktorida Khastini Risna Riza Firmansyah Riza Zainuddin Ahmad ROHANI CINTA BADIA GINTING Rohani Cinta Badia Ginting Sally SMITH Sihegiko Kanaya SIHEGIKO KANAYA Sintho Wahyuning Ardie Soekisman Tjitrosoedirdjo Sofi Mursidawati Sofi Mursidawati Sri Listiyowati Subagya, Mahmud Sukarno Sukarno Sukmarayu P Gedoan Sukmarayu P. Gedoan Sulfiah, Siti Syaiful Anwar UTUT WIDYASTUTI Utut Widyastuti UTUT WIDYASTUTI UTUT WIDYASTUTI Utut Widyastuti Utut Widyastuti Suharsono Utut Widyastuti Suharsono† Waite, Mashuri Wei Zhang Wendi Nurul Fadillah Wendi Nurul Fadillah Yanti Novera Yasuyuki Hashidoko† Yuyun Nisaul Khairillah Za’aziza Ridha Julia