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All Journal HAYATI Journal of Biosciences Jurnal Agronomi Indonesia (Indonesian Journal of Agronomy) Jurnal Ilmu Pertanian Indonesia Jurnal Teknologi Dan Industri Pangan Journal of Tropical Life Science : International Journal of Theoretical, Experimental, and Applied Life Sciences Microbiology Indonesia BIOTROPIA - The Southeast Asian Journal of Tropical Biology Jurnal Pengelolaan Sumberdaya Alam dan Lingkungan (Journal of Natural Resources and Environmental Management) Jurnal Sumberdaya Hayati JURNAL BIOLOGI INDONESIA ANNALES BOGORIENSES Biosaintifika: Journal of Biology & Biology Education Menara Perkebunan Floribunda Jurnal Ilmiah Sains Annales Bogorienses Biodiversitas Journal of Biological Diversity
Nampiah Sukarno
Department Of Biology, Faculty Of Mathematics And Natural Sciences, Institut Pertanian Bogor. Jl. Raya Dramaga, Kampus IPB Darmaga, Bogor 16680, West Java, Indonesia
Agriculture, Biological Sciences & Forestry Biochemistry, Genetics & Molecular Biology Chemical Engineering, Chemistry & Bioengineering Chemistry Computer Science & IT Earth & Planetary Sciences Economics, Econometrics & Finance Environmental Science Immunology & microbiology Industrial & Manufacturing Engineering Mathematics Medicine & Pharmacology Physics Veterinary

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IDENTIFICATION OF Aspergillus flavus AND DETECTION OF ITS AFLATOXIN GENES ISOLATED FROM PEANUT AND PEANUT PROCESSED PRODUCTS Kemala S. Nagur; Nampiah Sukarno; Sri Listiyowati
BIOTROPIA Vol. 21 No. 1 (2014): BIOTROPIA Vol. 21 No. 1 June 2014
Publisher : SEAMEO BIOTROP

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (164.864 KB) | DOI: 10.11598/btb.2014.21.1.398

Abstract

Aspergillus flavus is one of the main fungi that are able to produce aflatoxin.The presence of the fungi and its aflatoxin are become serious problem on food safety. This research was aimed to isolate and identify A. flavus from peanut and its processed products collected from some traditional markets in Bogor, Depok and Jakarta, and detection of their aflatoxin genes. Fungal isolation was using AFPA media. Fungal identification was carried out by combining morphological and molecular analysis used species specific primers FVAVIQ1/FLAQ2 and AFLA-F/AFLA-R, while detection of  aflatoxin genes employed four specific primers of apa-2 (aflR), nor-1 (aflD), ver-1 (aflM) and omt-1 (aflP). From 36 samples, the A. flavus group was only found in peanut kernels samples with viable count of specific colonies in the range of 0.01-5.52 x 10-4 cfu/g. The total 18 isolates were identified as A. flavus based on species specific primers FVAVIQ1/FLAQ2 and AFLA-F/AFLA-R by producing amplicons about 100 and 413 bp respectively. Based on aflatoxin gene analysis showed that all 18 isolates successfully amplified by both apa-2 and nor-1, 83.3 % by omt-1 and 72.2 % by ver-1 genes which taking part in aflatoxin production. The amplicons size of apa-2, nor-1, ver-1 and omt-1   primer pairs were about 1032, 400, 895 and 1024 bp respectively.
Growth inhibition of Hydrilla verticillata by freshwater fungi Triadiati Triadiati; Nampiah Sukarno; IRMA SITI RAHMAH
Biodiversitas Journal of Biological Diversity Vol. 22 No. 7 (2021)
Publisher : Society for Indonesian Biodiversity

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.13057/biodiv/d220739

Abstract

Abstract. Triadiati T, Sukarno N, Rahmah IS. 2021. Growth inhibition of Hydrilla verticillata by freshwater fungi. Biodiversitas 22: 2876-2882. The uncontrolled growth of hydrilla (Hydrilla verticillata (L.f.) Royle) in Mekarsari Fruit Garden, Bogor causes various losses. A Freshwater fungus is one of the alternatives to control hydrilla growth. Therefore, the study aimed to investigate the damage and growth inhibition of hydrilla using freshwater fungi. Freshwater fungi were isolated from Lake Mekarsari Fruit Garden. Hydrilla growth characteristics observed were stem length, stem nodus number, number of healthy leaves, leaf number, leaf damage, wet and dry weight. The results showed that a total of seven isolates of freshwater fungi were obtained from Lake Mekarsari Fruit Garden. Two species, i.e. Myrothecium sp. and Stachybotrys sp. were selected to control hydrilla growth. Fungal treatment reduced the stem length and leaf number of hydrilla. The combination of both fungal isolates showed less leaf damage than Myrothecium sp. The damage of hydrilla leaves by Myrothecium sp. and Stachybotrys sp. were 98.07% and 78.71%, respectively.
Root associated Fusarium solani Species Complex (FSSC) in epiphytic and terrestrial orchids NAMPIAH SUKARNO; SOFI MURSIDAWATI; SRI LISTIYOWATI; NAUFAL HAFIZH NUGRAHA; WENDI NURUL FADILLAH; MASHURI WAITE
Biodiversitas Journal of Biological Diversity Vol. 24 No. 5 (2023)
Publisher : Society for Indonesian Biodiversity

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.13057/biodiv/d240509

Abstract

Abstract. Sukarno N, Mursidawati S, Listiyowati S, Nugraha NH, Fadillah WN, Waite M. 2023. Root associated Fusarium solani Species Complex (FSSC) in epiphytic and terrestrial orchids. Biodiversitas 24: 2577-2586. Members of the Fusarium solani Species Complex (FSSC) are ecologically diverse, covering human and plant pathogens, saprobes, and endophytic fungi of economically important plants. The role of FSSC on tropical orchids, however, has received little attention. This research aimed to isolate and identify the FSSC found as endophytes in roots of the epiphytic orchids Cymbidium finlaysonianum Lindl. and Vanda tricolor Lindl., and the terrestrial orchids Calanthe triplicata (Willemet) Ames and Phaius tankervilleae (Banks) Blume. Fungal isolation was obtained from peloton structures within the root. Fungal identification was done using combined morphological and molecular characteristics using ITS rDNA sequences. Four isolates of Fusarium were identified based on morphological characteristics. The BLAST analysis showed that all the isolates were in the Fusarium solani Species Complex (FSSC). Further phylogenetic relationship analysis indicated that all the FSSC isolates belonged to FSSC5 lineage or Fusarium solani sensu stricto, which is nested in FSSC clade 3 as a subgroup. The fungi F. solani sensu stricto V2 and CF34 were isolated from the epiphytic orchids, and F. solani sensu stricto C5 and P44 were isolated from the terrestrial orchids. It is interesting that the FSSC5 isolated from the epiphytic orchids form different groups than those of the terrestrial orchids. This is the first report showing the tropical orchids are the host of the FSSC5 as endophytes and this broadens the known host range and ecological behavior of the FSSC5.
Co-Authors ADI YULANDI Adi Yulandi Agustin Wydia Gunawan ALEX HARTANA Alex Hartana Alex Hartana Anastastia Raditya Hidayanti Astri Ariyani Atit Kanti Atit Kanti Cici Rahmawati Cinta Badia Br Ginting, Rohani Dedi Fardiaz Desi Maria Panjaitan Didiek Hadjar Goenadi Dyah Iswantini Edhi Sandra Edi Guhardja Endang Sukara Eris Septiana Fachrian Hasmi Pasaribu Fachriyan Hasmi Pasaribu Fadillah, Wendi Nurul Franco, Christopher MM Hamim Hamim HAPPY WIDIASTUTI Hidayanti, Anastastia Raditya I Made Sudiana I Nyoman Pugeg Aryantha Ibnu Qayim IRMA SITI RAHMAH Irmanida Batubara Kartini Kramadibrata Kartini Kramadibrata Kemala S. Nagur L K Darusman Lady Diana Tetelepta, Lady Diana Latifah K Darusman latifah K Darusman Latifah K. Darusman LATIFAH KOSIM DARUSMAN Latifah Kosim Darusman Latifah Kosim Darusman M. Rafi MASHURI WAITE Mashuri Waite Mashuri Waite Maulani, Nail Izzatul Mega Putri Amelya Michael Michael Mien Ahmad Rifai Muhammad Hilman Aulia Mujahidah, Shofia Mujahidah, Shofia Mulya Sari Nadia T. Hendartina Nadiya Dwi Rahayu NAUFAL HAFIZH NUGRAHA Novriyandi Hanif Partomuan Simanjuntak Putra, Sukma Triperdana Rahayu Laelandi Rahminiwati, Min Rida Oktorida Khastini Rika Indri Astuti Risna Riza Firmansyah Riza Zainuddin Ahmad ROHANI CINTA BADIA GINTING Rohani Cinta Badia Ginting Sally SMITH SIHEGIKO KANAYA Sihegiko Kanaya Sintho Wahyuning Ardie Soekisman Tjitrosoedirdjo SOFI MURSIDAWATI Sofi Mursidawati Sofi Mursidawati Sri Listiyowati Subagya, Mahmud Sukarno Sukarno Sukmarayu P Gedoan Sukmarayu P. Gedoan Sulfiah, Siti Syaiful Anwar Triadiati Triadiati Utut Widyastuti UTUT WIDYASTUTI Utut Widyastuti UTUT WIDYASTUTI UTUT WIDYASTUTI Utut Widyastuti Suharsono Utut Widyastuti Suharsono† Waite, Mashuri Wei Zhang WENDI NURUL FADILLAH Wendi Nurul Fadillah Wendi Nurul Fadillah Wendi Nurul Fadillah Yanti Novera Yasuyuki Hashidoko† Yuyun Nisaul Khairillah Za’aziza Ridha Julia