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All Journal HAYATI Journal of Biosciences Jurnal Agronomi Indonesia (Indonesian Journal of Agronomy) Jurnal Ilmu Pertanian Indonesia Jurnal Teknologi Dan Industri Pangan Journal of Tropical Life Science : International Journal of Theoretical, Experimental, and Applied Life Sciences Jurnal Veteriner Microbiology Indonesia BIOTROPIA - The Southeast Asian Journal of Tropical Biology Jurnal Pengelolaan Sumberdaya Alam dan Lingkungan (Journal of Natural Resources and Environmental Management) Jurnal Sumberdaya Hayati JURNAL BIOLOGI INDONESIA ANNALES BOGORIENSES Biosaintifika: Journal of Biology & Biology Education Menara Perkebunan Floribunda Jurnal Ilmiah Sains Annales Bogorienses
Nampiah Sukarno
Department Of Biology, Faculty Of Mathematics And Natural Sciences, IPB University, Bogor, Indonesia
Agriculture, Biological Sciences & Forestry Biochemistry, Genetics & Molecular Biology Chemical Engineering, Chemistry & Bioengineering Chemistry Computer Science & IT Earth & Planetary Sciences Economics, Econometrics & Finance Environmental Science Immunology & microbiology Industrial & Manufacturing Engineering Mathematics Medicine & Pharmacology Physics Veterinary

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Cellulolytic Yeast Isolated From Raja Ampat Indonesia Kanti, Atit; Sukarno, Nampiah; Sukara, Endang; Darusman, Latifah K.
Annales Bogorienses Vol. 16 No. 1 (2012): Annales Bogorienses
Publisher : BRIN

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Abstract

The objective of this study was to select and characterize three yeast isolates originating from soil of Raja Ampat region of Papua, Indonesia for its potential to produce cellulase . Selection and characterization of cellulolytic yeast was carried out by measuring cellulolytic Index (IS)with congo red method and measurement of Carboxy Methyl Cellulase (CMC-ase) activity through determination of reducing sugar with dinitrosalycilic methods. Cellulolytic Index (IS) of the isolates Sporobolomyces poonsookiae Y08RA07, Rhodosporidium paludigenum Y08RA29, and Cryptococcus flavescens Y08RA33 were 1.40, 2.60 and 1.66, respectively. CMC-ase produced optimum at pH 8 at 37ºC by isolate Y08RA07; whereas for Y08RA29 and Y08RA33 were at pH 6, at 28ºC. Paper waste was good substrate for cellulase enzyme production by isolate Y08RA07, while for two other isolates the best substrate was CMC. Isolate Y08RA29 having highest cellulase activities when grown in CMC, while isolates Y08RA07 and Y08RA33 achieved highest enzyme activity when grown in bamboo leaf. 
IDENTIFICATION OF Aspergillus flavus AND DETECTION OF ITS AFLATOXIN GENES ISOLATED FROM PEANUT AND PEANUT PROCESSED PRODUCTS Nagur, Kemala S.; Sukarno, Nampiah; Listiyowati, Sri
BIOTROPIA Vol. 21 No. 1 (2014): BIOTROPIA Vol. 21 No. 1 June 2014
Publisher : SEAMEO BIOTROP

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (164.864 KB) | DOI: 10.11598/btb.2014.21.1.398

Abstract

Aspergillus flavus is one of the main fungi that are able to produce aflatoxin.The presence of the fungi and its aflatoxin are become serious problem on food safety. This research was aimed to isolate and identify A. flavus from peanut and its processed products collected from some traditional markets in Bogor, Depok and Jakarta, and detection of their aflatoxin genes. Fungal isolation was using AFPA media. Fungal identification was carried out by combining morphological and molecular analysis used species specific primers FVAVIQ1/FLAQ2 and AFLA-F/AFLA-R, while detection of  aflatoxin genes employed four specific primers of apa-2 (aflR), nor-1 (aflD), ver-1 (aflM) and omt-1 (aflP). From 36 samples, the A. flavus group was only found in peanut kernels samples with viable count of specific colonies in the range of 0.01-5.52 x 10-4 cfu/g. The total 18 isolates were identified as A. flavus based on species specific primers FVAVIQ1/FLAQ2 and AFLA-F/AFLA-R by producing amplicons about 100 and 413 bp respectively. Based on aflatoxin gene analysis showed that all 18 isolates successfully amplified by both apa-2 and nor-1, 83.3 % by omt-1 and 72.2 % by ver-1 genes which taking part in aflatoxin production. The amplicons size of apa-2, nor-1, ver-1 and omt-1   primer pairs were about 1032, 400, 895 and 1024 bp respectively.
Co-Authors , Triadiati ADI YULANDI Agustin Wydia Gunawan ALEX HARTANA Alex Hartana Alex Hartana Anastastia Raditya Hidayanti Astri Ariyani Atit Kanti Atit Kanti Cici Rahmawati Cinta Badia Br Ginting, Rohani Dedi Fardiaz Desi Maria Panjaitan Didiek Hadjar Goenadi Dyah Iswantini Edhi Sandra Edi Guhardja Endang Sukara Eris Septiana Fachrian Hasmi Pasaribu Fachriyan Hasmi Pasaribu Fadillah, Wendi Nurul Franco, Christopher MM Hamim Hamim HAPPY WIDIASTUTI Hidayanti, Anastastia Raditya I Made Sudiana I Nyoman Pugeg Aryantha Ibnu Qayim Irmanida Batubara Kartini Kramadibrata Kemala S. Nagur L K Darusman Lady Diana Tetelepta, Lady Diana latifah K Darusman Latifah K Darusman Latifah K. Darusman LATIFAH KOSIM DARUSMAN Latifah Kosim Darusman Latifah Kosim Darusman M. Rafi M. Sukarno Mashuri Waite Mashuri Waite Maulani, Nail Izzatul Mega Putri Amelya Michael Michael Mien Ahmad Rifai Mujahidah, Shofia Mujahidah, Shofia Mulya Sari Nadia T. Hendartina Nadiya Dwi Rahayu Novriyandi Hanif Partomuan Simanjuntak Putra, Sukma Triperdana Rahayu Laelandi Rahayu, Nadiya Dwi Rahminiwati, Min Rida Oktorida Khastini Risna Riza Firmansyah Riza Zainuddin Ahmad ROHANI CINTA BADIA GINTING Rohani Cinta Badia Ginting Sally SMITH SIHEGIKO KANAYA Sihegiko Kanaya Sintho Wahyuning Ardie Soekisman Tjitrosoedirdjo Sofi Mursidawati Sofi Mursidawati Sri Listiyowati Subagya, Mahmud Sukarno Sukarno Sukmarayu P Gedoan Sukmarayu P. Gedoan Sulfiah, Siti Syaiful Anwar UTUT WIDYASTUTI Utut Widyastuti UTUT WIDYASTUTI Utut Widyastuti UTUT WIDYASTUTI Utut Widyastuti Suharsono Utut Widyastuti Suharsono† Waite, Mashuri Wei Zhang Wendi Nurul Fadillah Wendi Nurul Fadillah Yanti Novera Yasuyuki Hashidoko† Yuyun Nisaul Khairillah Za’aziza Ridha Julia