Garuda - Garba Rujukan Digital

Saltwater Fish Nanoparticles: Biological Effects on COL1A1 Expression in Fetal Mice and Tablet Formula Optimization Christiono, Sandy; Hutami, Islamy Rahma; Suparmi, Suparmi; Afroz, Shaista; Clarasya, Amara Danish; Ahmad, Rayyan Orvin Pradipta; Subiyono, Amelia Febriani
Jurnal Ilmiah Perikanan dan Kelautan 2026: JIPK VOLUME 18 ISSUE 1 YEAR 2026 (FEBRUARY 2026, ISSUE IN PROGRESS)
Publisher : Faculty of Fisheries and Marine Universitas Airlangga

Show Abstract | Download Original | Original Source | Check in Google Scholar

Graphical Abstract Highlight Research Saltwater fish Nano powder modulates the expression of matrix proteins, influencing early dental tissue development. Administration significantly decreases COL1A1 expression in fetal mouse teeth, affecting biomineralization. Increasing talc concentration improves flowability and formulation stability by reducing angle of repose, Carr’s index, and Hausner ratio. Findings suggest marine-derived nanoparticles regulate cellular differentiation during dental development. Supports potential applications of marine natural materials in prenatal nutrition and dental tissue engineering. Abstract Saltwater fish nanoparticle-based tablets represent a novel nutritional strategy aimed enhancing dental hard tissue density, particularly enamel. These tablets incorporate bioactive proteins, omega-3 fatty acids, and essential minerals such as calcium, phosphate, and magnesium to support enamel biomineralization. The nanoparticle formulation facilitates efficient cellular absorption, thereby increasing the expression of key enamel proteins like collagen type I alpha 1 chain (COL1A1) during enamel matrix development. This study investigated the effect of saltwater fish nanoparticles on COL1A1 expression in ameloblast cells and evaluated the physicochemical properties of tablets with talc concentrations of 1%, 5%, and 10%. Using a true experimental design with a post-test only control group, two groups of mice were assigned: a control group fed standard diet, and a treatment group fed a standard diet supplemented with saltwater fish nanoparticles (2.17 mg/0.5 mL). Tablet formulations were analyzed across the three talc concentrations. Data were subjected to independent T-tests for COL1A1 expression and One-way ANOVA for physicochemical properties. Results revealed a significant reduction in COL1A1 expression in the treatment group. Additionally, talc concentration significantly influenced tablet physicochemical characteristics, with the 10% talc formulation exhibiting the most optimal properties. These findings suggest potential for nanoparticle-based nutritional interventions to promote enamel formation.

Saltwater Fish Powder for Amelogenesis in Zebrafish Larvae (Danio rerio) Christiono, Sandy; Pradopo, Seno; Hutami, Islamy Rahma; Indasari, Novia; Nurhapsari, Arlina; Rochmah, Yayun Siti Rochmah; Ibrahim, Zurairah; Yuniar, Savira Nurazky; Suparmi
Jurnal Ilmiah Perikanan dan Kelautan Vol. 16 No. 2 (2024): JURNAL ILMIAH PERIKANAN DAN KELAUTAN
Publisher : Faculty of Fisheries and Marine Universitas Airlangga

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20473/jipk.v16i2.54677

Graphical Abstract Highlight Research 1. The saltwater fish powder is rich in omega3, vitamin D, and calcium which helps tooth amelogenesis. 2. LC50 Toxicity Test was carried out using zebrafish larvae (Danio rerio) with negative control, positive control using 3,4-dichloroanilin, and treatment groups with saltwater fish powder solution in 6 different concentration. 3. No signs of zebrafish embryo mortality or developmental abnormalities on treatment group from the lowest (125Î¼g/ml) nor highest concentration (4000Î¼g/ml). 4. Saltwater fish powder has proven lack of toxicity. Abstract Many nutrients found in saltwater fish are thought to promote amelogenesis in ameloblast cells during tooth development. Although its toxicity is uncertain, saltwater fish powder provided benefits for amelogenesis. Zebrafish embryos have complex metabolic pathways and comprehensive physiological reactions. The LC50 toxicity of saltwater fish powder on zebrafish embryos was investigated in this study. The aim of the of the study is to determine the effect of LC50 toxicity on saltwater fish powder on zebrafish embryos. The experimental research method was a post-test-only control group design, consisting of eight groups, namely the negative control and the internal group using embryo media, the positive control using 3,4-dichloroanillin, and the treatment group 125Î¼g / ml, 250Î¼g / ml, 500Î¼g / ml, 1000Î¼g / ml, 2000Î¼g / ml, and 4000Î¼g / ml using 384 zebrafish embryos. Saltwater fish powder is diluted and observed for 96 hours, controlled every 24 hours. It was found that saltwater fish powder was not toxic because there were no dead zebrafish embryos from various concentrations, negative control, and internal control.

Optimasi Program Usaha Kesehatan Gigi Sekolah (UKGS) Melalui Edukasi pada Dokter Gigi Kecil di SD IT Asshodiqiyah Semarang. Christiono, Sandy; Amalina, Rizki; Pradopo, Seno; Yuniar, Savira Nurazky
Karya Kesehatan Journal of Community Engagement Vol 5 No 1 (2024): Karya Kesehatan Journal of Community Engangement
Publisher : Sekolah Tinggi Ilmu Kesehatan Karya Kesehatan

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.46233/k2jce.v5i1.1063

Abstrak. Kesehatan gigi dan mulut merupakan hal yang penting dalam kehidupan. Tingginya angka karies pada usia sekolah dasar menunjukkan pentingnya pemberian edukasi guna membentuk kebiasaan baik untuk menjaga kesehatan gigi. Kegiatan pengabdian masyarakat ini bertujuan untuk mengedukasi siswa usia sekolah dasar melalui pendekatan Usaha Kesehatan Gigi Sekolah (UKGS) yang melibatkan siswa sebagai dokter gigi kecil. Kegiatan dilaksanakan pada 28 Oktober 2023. Kegiatan ini dilaksanakan melalui kaderisasi dokter gigi kecil di SD IT Asshodiqiyah Semarang yang nantinya akan memberikan edukasi kepada teman sebayanya. Tingkat pengetahuan siswa diukur menggunakan kuesioner dengan 10 pertanyaan singkat terkait materi yang diberikan, kemudian dibandingkan antara skor sebelum dan setelah pemberian edukasi oleh dokter gigi kecil. Didapatkan adanya perbedaan signifikan tingkat pengetahuan siswa antara skor pre-test dan post-test pada seluruh responden sehingga metode ini dianggap efektif dalam meningkatkan pengetahuan terkait kesehatan gigi dan mulut pada siswa SD IT As-Shodiqiyah sebagai upaya optimasi program UKGS di sekolah. Abstract. Oral health is an important thing in life. The high rate of caries at elementary school age shows the importance of providing dental health education to form good habits to maintain oral health. This community service activity aims to give dental health education through the Usaha Kesehatan Gigi Sekolah (UKGS) programme approach which involves students as little dentists. This activity is carried out through the cadre of 2 little dentists at SD IT Asshodiqiyah Semarang who will later provide dental health education to their peers. The level of student knowledge was measured using a questionnaire with 10 short questions, then compared between the scores before (pre-test) and after (post-test) providing education by the little dentist. It was found that there was a significant difference in the level of student knowledge between the pre-test and post-test scores for all respondents, this method was considered effective in increasing knowledge related to dental and oral health in students at SD As-Shodiqiyah to optimize the “UKGS’ program at school.

EFFECT OF FIBER VOLUME ON ELASTIC MODULUS OF POST-CORE FABRICATED REINFORCED COMPOSITE Benyamin, Benni; Ekoningtyas, Endah Aryati; Christiono, Sandy; Fatmawati, Dwi Warna Ayu
Odonto : Dental Journal Vol 10, No 2 (2023): December 2023
Publisher : Faculty of Dentistry, Universitas Islam Sultan Agung

Show Abstract | Download Original | Original Source | Check in Google Scholar

Background: Post-core restoration is one restoration after endodontic treatment by optimizing the root canal as a retention. Fracture of the root is the most common failure due to the post being less elastic in holding the chewing load, especially the metal post. FRC post-core indicates to prevent tooth fracture. In addition, the FRC post-core minimizes the reduction of dentine tissue in the root canals, called a minimally invasive dentistry approach. This study examined the effect of the number of fibres on the elastic moduli of fabricated FRC posts using a three-bending point test to determine the elasticity of fabricated FRC posts. Method: The study was experimental laboratory research with the post-only control group design using polyethene fibre (Kerr) and flowable composite resin (Kerr), prefabricated post-core (Kerr). The study was divided into four groups, namely prefabricated, fabricated with a volume of 1 fibre, 2 fibres, and 3 fibres, where each group consisted of 6 samples. Result: There was no significant difference in the magnitude of the force on the specimen (p>0.05). The force among the groups was the same, the alteration (deflection) of the specimens among the groups was significantly different (p

EFFECT OF FIBER VOLUME ON ELASTIC MODULUS OF POST-CORE FABRICATED REINFORCED COMPOSITE Benyamin, Benni; Ekoningtyas, Endah Aryati; Christiono, Sandy; Fatmawati, Dwi Warna Ayu
Odonto : Dental Journal Vol 10, No 2 (2023): December 2023
Publisher : Faculty of Dentistry, Universitas Islam Sultan Agung

Show Abstract | Download Original | Original Source | Check in Google Scholar

Background: Post-core restoration is one restoration after endodontic treatment by optimizing the root canal as a retention. Fracture of the root is the most common failure due to the post being less elastic in holding the chewing load, especially the metal post. FRC post-core indicates to prevent tooth fracture. In addition, the FRC post-core minimizes the reduction of dentine tissue in the root canals, called a minimally invasive dentistry approach. This study examined the effect of the number of fibres on the elastic moduli of fabricated FRC posts using a three-bending point test to determine the elasticity of fabricated FRC posts. Method: The study was experimental laboratory research with the post-only control group design using polyethene fibre (Kerr) and flowable composite resin (Kerr), prefabricated post-core (Kerr). The study was divided into four groups, namely prefabricated, fabricated with a volume of 1 fibre, 2 fibres, and 3 fibres, where each group consisted of 6 samples. Result: There was no significant difference in the magnitude of the force on the specimen (p>0.05). The force among the groups was the same, the alteration (deflection) of the specimens among the groups was significantly different (p

Amelogenin and alkaline phosphatase expression in ameloblast after saltwater fish consumption in pregnant mice (Mus musculus) Christiono, Sandy; Pradopo, Seno; Sudiana, I Ketut; Hutami, Islamy Rahma; Mayangsari, Regilia Shinta; Rochmah, Yayun Siti; Ibrahim, Zurairah
Dental Journal (Majalah Kedokteran Gigi) Vol. 56 No. 3 (2023): September
Publisher : Faculty of Dental Medicine, Universitas Airlangga https://fkg.unair.ac.id/en

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20473/j.djmkg.v56.i3.p166-171

Background: The intricate process of tooth formation during embryonic development ensures sufficient nutrition for the growth of healthy dental tissues. Amelogenin and alkaline phosphatase (ALP) are serine proteinases secreted by the ameloblast during the transition and maturation phases of the amelogenesis process. Consumption of saltwater fish is predicted to increase the expression of amelogenin and ALP in ameloblast cells during tooth formation. Only now have the function of each gene, tooth-forming cells, and the proteins they produce in the biomolecular amelogenesis of tooth enamel, which began during prenatal development, been clarified. Purpose: This study aims to determine how saltwater fish powder affects the ability of mother mice to increase the expression of amelogenin and ALP in cell ameloblast. Methods: Using a completely randomized design, this study was experimental and aimed to examine the effects of sardine (Sardinella fimbriata), splendid ponyfish (Leiognathus splendens), and tuna (Euthynnus affinis) powder. As samples, twenty-four female mice (Mus musculus) were used. Two groups of mice were created: group 1 (2.14 mg/0.5 ml) and the control group. The expression of amelogenin and ALP was determined using immunohistochemistry (IHC) and t-test (p0.05). Results: Expression of ameloblast was significantly different between the treatment and control groups (p0.05). Conclusion: The consumption of saltwater fish reduces the amelogenin and ALP expressions of mouse fetal ameloblast cells during tooth development in vivo.

Title

Found 46 Documents
Search

Abstract

Abstract

Abstract

Abstract

Abstract

Abstract

Title Search

Found 46 Documents Search

Abstract

Abstract

Abstract

Abstract

Abstract

Abstract

Title

Found 46 Documents
Search