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Amelogenin and alkaline phosphatase expression in ameloblast after saltwater fish consumption in pregnant mice (Mus musculus) Christiono, Sandy; Pradopo, Seno; Sudiana, I Ketut; Hutami, Islamy Rahma; Mayangsari, Regilia Shinta; Rochmah, Yayun Siti; Ibrahim, Zurairah
Dental Journal (Majalah Kedokteran Gigi) Vol. 56 No. 3 (2023): September
Publisher : Faculty of Dental Medicine, Universitas Airlangga https://fkg.unair.ac.id/en

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20473/j.djmkg.v56.i3.p166-171

Background: The intricate process of tooth formation during embryonic development ensures sufficient nutrition for the growth of healthy dental tissues. Amelogenin and alkaline phosphatase (ALP) are serine proteinases secreted by the ameloblast during the transition and maturation phases of the amelogenesis process. Consumption of saltwater fish is predicted to increase the expression of amelogenin and ALP in ameloblast cells during tooth formation. Only now have the function of each gene, tooth-forming cells, and the proteins they produce in the biomolecular amelogenesis of tooth enamel, which began during prenatal development, been clarified. Purpose: This study aims to determine how saltwater fish powder affects the ability of mother mice to increase the expression of amelogenin and ALP in cell ameloblast. Methods: Using a completely randomized design, this study was experimental and aimed to examine the effects of sardine (Sardinella fimbriata), splendid ponyfish (Leiognathus splendens), and tuna (Euthynnus affinis) powder. As samples, twenty-four female mice (Mus musculus) were used. Two groups of mice were created: group 1 (2.14 mg/0.5 ml) and the control group. The expression of amelogenin and ALP was determined using immunohistochemistry (IHC) and t-test (p0.05). Results: Expression of ameloblast was significantly different between the treatment and control groups (p0.05). Conclusion: The consumption of saltwater fish reduces the amelogenin and ALP expressions of mouse fetal ameloblast cells during tooth development in vivo.

ORAL AND DENTAL MANAGEMENT IN CHILDREN WITH TETRALOGY OF FALLOT : A LITERATURE REVIEW Anggarani, Welly; Christiono, Sandy; Agusmawanti, Prima
Odonto : Dental Journal Vol 8, No 1 (2021): July 2021
Publisher : Faculty of Dentistry, Universitas Islam Sultan Agung

Background: Congenital heart disease is a condition of heart anomaly and or disfunction of heart since birth. The most common congenital heart disease is Tetralogy Of Fallot (TOF). The disorder has four features, a ventricular septal defect, aortic overriding, stenosis pulmonary, and hypertrophy right ventricular.Method: This review aimed to talk about oral manifestations and discuss options of several oral and dental management in children with TOF.Result: Oral and dental treatment of pediatric patient with cardiovascular disease is important to be carried out with the risk of bacterial endocarditis.Conclusion: Oral and dental management in a child with congenital heart disease is very important. Main point of dental management are dental health education, preventive dental treatment and control periodic after dental treatment.

PENGARUH SUSU IBU HAMIL TERHADAP EKSPRESI KALLIKREIN-RELATED PEPTIDASE-4 (KLK-4) PADA SEL AMELOBLAS DALAM TUMBUH KEMBANG GIGI Samia, Salma; Christiono, Sandy; Sari, Rina Kartika
Odonto : Dental Journal Vol 7, No 2 (2020): December 2020
Publisher : Faculty of Dentistry, Universitas Islam Sultan Agung

Background: Tooth development during the embryonic period is a complex process that needs enough nutrition for the formation of healthy dental tissue. Kallikrein-related peptidase-4 (KLK-4) is a serine proteinase secreted by ameloblast during transition and maturation stages in the amelogenesis process which functions to degrade protein matrices so enamel could reach the final hardness. Pregnancy milk contains various nutrients that can increase KLK-4 expression of ameloblast cells in the tooth development. This study aims to determine the impact of pregnancy milk against the KLK-4 expression of ameloblast cell in the tooth development process.Method: 12 pregnant female mice (Mus Musculus L.) divided into control group (given sterile aquadest) and treatment group (given pregnancy milk + sterile aquadest) for 18 days then the tooth germ was taken. Samples were stained to see the KLK-4 expression. Data were analyzed using an Independent sample t-test.Result: Our study showed that the average amounts of KLK-4 expression in the treatment group are higher than the control group. Based on the Independent sample t-test statistic methods, there is a significant difference with the value of p=0,017.Conclusion: The pregnancy milk could influence the Kallikrein-related peptidase-4 (KLK-4) expression of ameloblast cell in the mice fetusâ€™s tooth development.

PENGARUH SUSU IBU HAMIL TERHADAP EKSPRESI KALLIKREIN-RELATED PEPTIDASE-4 (KLK-4) PADA SEL AMELOBLAS DALAM TUMBUH KEMBANG GIGI Samia, Salma; Christiono, Sandy; Sari, Rina Kartika
Odonto : Dental Journal Vol 7, No 2 (2020): December 2020
Publisher : Faculty of Dentistry, Universitas Islam Sultan Agung

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.30659/odj.7.2.98-103

Background: Tooth development during the embryonic period is a complex process that needs enough nutrition for the formation of healthy dental tissue. Kallikrein-related peptidase-4 (KLK-4) is a serine proteinase secreted by ameloblast during transition and maturation stages in the amelogenesis process which functions to degrade protein matrices so enamel could reach the final hardness. Pregnancy milk contains various nutrients that can increase KLK-4 expression of ameloblast cells in the tooth development. This study aims to determine the impact of pregnancy milk against the KLK-4 expression of ameloblast cell in the tooth development process.Method: 12 pregnant female mice (Mus Musculus L.) divided into control group (given sterile aquadest) and treatment group (given pregnancy milk + sterile aquadest) for 18 days then the tooth germ was taken. Samples were stained to see the KLK-4 expression. Data were analyzed using an Independent sample t-test.Result: Our study showed that the average amounts of KLK-4 expression in the treatment group are higher than the control group. Based on the Independent sample t-test statistic methods, there is a significant difference with the value of p=0,017.Conclusion: The pregnancy milk could influence the Kallikrein-related peptidase-4 (KLK-4) expression of ameloblast cell in the mice fetusâ€™s tooth development.

Program edukasi “Mata Jeli, Gigi Sehat”: Meningkatkan pengetahuan deteksi dini karies pada remaja Sandy Christiono; Welly Anggarani; Muhammad Dian Firdausy; Amara dhanish Clarasya
Community Empowerment Journal Vol. 4 No. 2 (2026)
Publisher : CV. Yudhistt Fateeh

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.61251/cej.v4i2.380

Karies gigi merupakan masalah kesehatan mulut yang prevalensinya masih tinggi di Indonesia, khususnya pada kelompok remaja. Mayoritas kasus baru terdeteksi pada stadium lanjut karena minimnya pengetahuan tentang tanda-tanda awal karies, termasuk lesi bercak putih (white spot lesion) yang pada tahap ini sesungguhnya masih bersifat reversibel. Program pengabdian kepada masyarakat ini bertujuan untuk meningkatkan pengetahuan siswa SMK Al-Sya’iriyah, Limpung, Batang, tentang deteksi dini karies gigi melalui program edukasi interaktif “Mata Jeli, Gigi Sehat”. Kegiatan dilaksanakan pada 12 Juni 2025 dengan desain one-group pre-post test yang melibatkan 35 siswa. Intervensi terdiri atas empat sesi edukasi yang mencakup identifikasi visual karies tahap awal, pengenalan gejala non-visual, konsep remineralisasi, serta opsi perawatan preventif. Data dianalisis menggunakan uji paired t-test untuk perbandingan nilai pengetahuan dan uji Wilcoxon signed-rank untuk perbandingan distribusi kategori. Rerata nilai pretest sebesar 47,43 ± 14,42 meningkat secara signifikan menjadi 78,29 ± 14,35 pada posttest, dengan rerata peningkatan 30,86 ± 5,07. Uji paired t-test menunjukkan perbedaan yang sangat signifikan [t(34) = 36,01, p < 0,001] dengan ukuran efek yang sangat besar (Cohen’s d = 2,14). Uji Wilcoxon mengonfirmasi perubahan distribusi kategori yang signifikan (p < 0,001), di mana proporsi siswa berkategori “tinggi” meningkat dari 0% menjadi 62,9%. Program edukasi interaktif berbasis visual ini terbukti efektif meningkatkan pengetahuan deteksi dini karies pada remaja, mendukung paradigma kedokteran gigi minimal invasif. Dental caries remains a highly prevalent oral health problem in Indonesia, particularly among adolescents. Most cases are detected at advanced stages due to limited awareness of early caries signs, including white spot lesions, which are still reversible at that stage. This community service program aimed to improve the knowledge of students at SMK Al-Sya’iriyah, Limpung, Batang, regarding early dental caries detection through an interactive educational program titled “Sharp Eyes, Healthy Teeth” (Mata Jeli, Gigi Sehat). The program employed a one-group pre-post test design involving 35 students on June 12, 2025. The intervention comprised four educational sessions covering visual identification of early caries, non-visual symptoms, remineralization principles, and preventive treatment options. Data were analyzed using a paired t-test for score comparison and the Wilcoxon signed-rank test for categorical distribution. Mean pre-test scores (47.43 ± 14.42) increased significantly to 78.29 ± 14.35 at post-test, with a mean improvement of 30.86 ± 5.07. The paired t-test demonstrated a highly significant difference [t(34) = 36.01, p < 0.001] with a very large effect size (Cohen’s d = 2.14). The Wilcoxon test confirmed a significant shift in knowledge category distribution (p < 0.001), with the proportion of students in the “high” category increasing from 0% to 62.9%. The visual-based interactive educational program was effective in significantly improving adolescents’ early dental caries detection knowledge, supporting the minimal invasive dentistry paradigm.

Dental And Oral Health Of Students With Intellectual Disabilities Sandy Christiono; Muhammad Dian Firdausy; Grahita Aditya; Budi Suhartono; Erwid Fatchur Rahman; Nira Ardlina; Adinda Lintang Pambayun; Amara Danish Clarasya
Jurnal Medali Vol 8, No 1 (2026): Media Dental Intelektual February 2026
Publisher : Faculty of Dentistry, Universitas Islam Sultan Agung

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.30659/medali.8.1.14-19

Background: Dental and oral health refers to the condition of the oral cavity and periodontal tissues being free from disease and pain. Optimal function of these structures indicates good oral health. Dental and oral health status can be expressed by the prevalence of dental caries and periodontal disease, assessed through indices such as def-t/DMF-T, OHI-S, and Gingival Index. This study aimed to determine the levels of def-t/DMF-T, OHI-S, and Gingival Index, and to describe the overall dental and oral health status among students with intellectual disabilities at Grobogan State Special SchoolMethod: This study employed a descriptive observational design with a non-experimental, cross-sectional approach to describe existing conditions through direct observation. A total of 52 students with intellectual disabilities were included using a total sampling technique.Result: DMF-T/def-t examination showed unsatisfactory findings, with the highest proportion of respondents categorized as very high (30.77%) and high (21.15%). The OHI-S examination indicated a moderate level, with most respondents (44.23%) falling within this category. The Gingival Index revealed generally good results, with the largest proportion categorized as normal (46.15%).Conclusion: Highest percentages of OHI-S and Gingival Index were found among students with moderate physical intellectual disabilities, while the highest def-t/DMF-T scores (very high category) were found among those with sensory intellectual disabilities

PROPOLIS EXTRACT AS ANTI-CORROSIVE AGENT INHIBITS METAL IONIC RELEASE FROM ORTHODONTIC BRACKETS Grahita Aditya; Erdianto Setya Wardhana; Islamy Rahma Hutami; Sandy Christiono; Rosa Pratiwi
Dentino: Jurnal Kedokteran Gigi Vol 9, No 1 (2024)
Publisher : FKG ULM

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20527/dentino.v9i1.18868

Background: A combination of nickel (Ni), chromium (Cr), and iron (Fe) can be found in the stainless steel orthodontic bracket. Ion release takes place as a result of the chemical interaction between metals and the oral environment, which leads to the potential of hypersensitivity during fixed orthodontic treatment. Propolis contains phytochemicals that have the ability to reduce corrosion, making it a potential treatment option. Purpose: to determine the release of Ni, Cr, and Fe in metal brackets in an 8.2% propolis solutionMethod: Four sets of six brackets were made from 24 brackets. Artificial saliva, sodium fluoride, sterilized distilled water (aquabidest), and 8.2% propolis were applied to these brackets. After incubating at 37°C for seven days, ICP EOS measured the metal ions Ni, Cr, and Fe. The analysis revealed significant differences (p<0.05) in ionic release of Ni, Cr, and Fe among groups exposed to sterilized distilled water (aquabidest) and an 8.2% propolis solution. Additionally, the Mann-Whitney test showed substantial Ni ion release differences (p<0.05) between 8.2% propolis solution and sterilized distilled water. The 8.2% propolis solution and sodium fluoride solution released Cr ions differently. Fe ion levels differed between propolis solution 8.2%, sodium fluoride solution, sterilized distilled water, and fake saliva. Conclusion: Propolis affects metal bracket ionic release of Ni, Cr, and Fe. Keywords : Cr and Fe, ions of Ni, propolis solution, Stainless steel bracket

INFLUENCE OF SERVICE QUALITY ON PATIENT SATISFACTION IN PRIMARY DENTAL HEALTHCARE SERVICES Erdianto Setya Wardhana; Kusuma Arbianti; Nira Ardlina; Sandy Christiono; Fauziah Illiyatul Wakhidah
Dentino: Jurnal Kedokteran Gigi Vol 11, No 1 (2026)
Publisher : FKG ULM

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20527/dentino.v11i1.26089

Background: Community Health Centers (Puskesmas) serve as primary healthcare providers in Indonesia, delivering essential medical services including dental and oral care for communities, families, and individuals. High-quality services emphasize five key dimensions of service quality: assurance, empathy, tangibles, reliability, and responsiveness. Purpose: This study aimed to examine the effect of service quality on patient satisfaction at the Dental Clinic of Mijen I Community Health Center. Methods: A concurrent mixed-methods design was applied using purposive sampling, with 142 patients as respondents. The independent variable was service quality, while the dependent variable was patient satisfaction. Data were collected through questionnaires and interview guidelines, and analyzed using validity and reliability testing, descriptive statistics, Spearman’s correlation, and ordinal regression. Results: The univariate analysis showed the highest score in assurance (90%) and the lowest in empathy and tangibles (88%). The average level of patient satisfaction was 85%. Bivariate analysis indicated positive correlations between service quality dimensions and satisfaction, with coefficients ranging from 0.207 to 0.347, the strongest being responsiveness. Multivariate analysis demonstrated that service quality simultaneously influenced patient satisfaction, with a Nagelkerke R² value of 0.381, meaning that 38.1% of satisfaction was explained by the five service quality dimensions, while the remaining 61.9% was influenced by other factors not examined in this study. Conclusion: Service quality significantly contributes to patient satisfaction, particularly through responsiveness, assurance, and reliability. Strengthening these dimensions is crucial to improving patient experiences at community-based dental health facilities.Keywords: Dental, Healthcare, Patient Satisfactio, Service Quality

Saltwater Fish Nanoparticles: Biological Effects on COL1A1 Expression in Fetal Mice and Tablet Formula Optimization Sandy Christiono; Islamy Rahma Hutami; Suparmi Suparmi; Shaista Afroz; Amara Danish Clarasya; Rayyan Orvin Pradipta Ahmad; Amelia Febriani Subiyono
Jurnal Ilmiah Perikanan dan Kelautan Vol. 18 No. 1 (2026): JURNAL ILMIAH PERIKANAN DAN KELAUTAN
Publisher : Faculty of Fisheries and Marine Universitas Airlangga

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20473/jipk.v18i1.76746

Graphical Abstract Highlight Research Saltwater fish Nano powder modulates the expression of matrix proteins, influencing early dental tissue development. Administration significantly decreases COL1A1 expression in fetal mouse teeth, affecting biomineralization. Increasing talc concentration improves flowability and formulation stability by reducing angle of repose, Carr’s index, and Hausner ratio. Findings suggest marine-derived nanoparticles regulate cellular differentiation during dental development. Supports potential applications of marine natural materials in prenatal nutrition and dental tissue engineering. Abstract Saltwater fish nanoparticle-based tablets represent a novel nutritional strategy aimed enhancing dental hard tissue density, particularly enamel. These tablets incorporate bioactive proteins, omega-3 fatty acids, and essential minerals such as calcium, phosphate, and magnesium to support enamel biomineralization. The nanoparticle formulation facilitates efficient cellular absorption, thereby increasing the expression of key enamel proteins like collagen type I alpha 1 chain (COL1A1) during enamel matrix development. This study investigated the effect of saltwater fish nanoparticles on COL1A1 expression in ameloblast cells and evaluated the physicochemical properties of tablets with talc concentrations of 1%, 5%, and 10%. Using a true experimental design with a post-test only control group, two groups of mice were assigned: a control group fed standard diet, and a treatment group fed a standard diet supplemented with saltwater fish nanoparticles (2.17 mg/0.5 mL). Tablet formulations were analyzed across the three talc concentrations. Data were subjected to independent T-tests for COL1A1 expression and One-way ANOVA for physicochemical properties. Results revealed a significant reduction in COL1A1 expression in the treatment group. Additionally, talc concentration significantly influenced tablet physicochemical characteristics, with the 10% talc formulation exhibiting the most optimal properties. These findings suggest potential for nanoparticle-based nutritional interventions to promote enamel formation.

The Relationship Of Nutritional Status and Plaque Bacteria On Children's Teeth Ages 5-12 Years Nabilla Marsha Diva; Welly Anggarani; Prima Agusmawanti; Sandy Christiono; Rizki Amalina
Jurnal Medali Vol 8, No 2 (2026): Media Dental Intelektual : August 2026
Publisher : Faculty of Dentistry, Universitas Islam Sultan Agung

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.30659/medali.8.2.102-106

Background: Nutritional status is a crucial factor in early growth, affecting weight and height. Adequate intake is essential for optimal growth, but imbalances can cause biological dysfunction of saliva to increase plaque accumulation. A study aimed to determine the relationship between nutritional status and plaque bacteria in children's teeth.Method: The study utilized laboratory experimental research with 100 children aged 5-12 years, using purposive sampling techniques. The sample was a dental plaque swab on the cervical lingual of the first molar tooth, and the total number of bacteria was counted using the colony counter.Result: The Spearman’s Rank correlation test obtained p>0.068 results showing the unsignificant correlation between nutritional status and the total number of plaque bacteria on children’s teeth.Conclusion: The study showed the overall amount of plaque bacteria on the children’s teeth decreased with improved nutrion intake and nutritional status.

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