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The Ameliorating Effect of Artificial Coffee from Mangrove Fruits (Rhizophora mucronata) on T Lymphocyte Cells and Renal Histopathology of BALB/c Mice Induced by Lipopolysaccharide Cahyono, Eko; Tanod, Wendy Alexander; Ijong, Frans Gruber; Ansar, Novalina Maya Sari; Mandeno, Jefri Anthonius; Karimela, Ely John; Rieuwpassa, Frets Jonas; Palawe, Jaka Frianto Putra; Sambeka, Yana; Setiawati, Natalia Prodiana
Squalen, Buletin Pascapanen dan Bioteknologi Kelautan dan Perikanan Vol 19, No 1 (2024): May 2024
Publisher : :Agency for Marine and Fisheries Research and Human Resources, Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.15578/squalen.835

Abstract

The potential of Rhizophora mucronata mangrove fruits as antioxidants is noteworthy, with promising prospects as nutraceuticals, particularly in their role as immunomodulators for T lymphocyte cells. R. mucronata fruits have been utilized by household industries in Indonesia as a coffee-like drink (artificial coffee). This study aims to determine the ability of mangrove fruit artificial coffee R. mucronata to stimulate T lymphocyte cells and renal histopathology conditions in LPS-induced mice animal models. This study also characterizes proximate characteristics, antioxidant capacity, and Gas Chromatography-Mass Spectrometry (GC-MS) screening of mangrove fruit artificial coffee compounds. The stages of this research included the process of mangrove fruit artificial coffee; analysis of proximate and caffeine; compounds screening (GC-MS) and toxicity prediction (Pro-Tox II); antioxidant capacity (DPPH); T lymphocyte cell expression (flow cytometry); and renal histopathological evaluation. The results showed moisture content (5.22±0.20%); ash (5.17±0.27%); protein (13.74±0.24%); fat (11.63±0.32%); carbohydrate (64.23±0.15%), caffeine (1.09±0.04%). GC-MS analysis showed that the compounds contained in artificial coffee were derivatives of fatty aldehyde, fatty acid, and cycloalkanes compound classes. Predicted toxicity of artificial coffee LD50 5000 mg/kg. Antioxidant capacity is classified as moderate (IC50 119.41±0.99µg/mL). Mangrove fruit artificial coffee can increase the relative number of CD4+CD62L+ by 14.68-29.48% and CD8+CD62L+ T lymphocyte cells by 12.60-30.33%. Artificial coffee can also increase the number of healthy cells and reduce the cells that undergo necrosis in the renal of LPS-induced mice. This study concluded that mangrove fruit artificial coffee R. mucronata positively affects T lymphocyte activation and mice renal cells’ protection from necrosis due to LPS induction. 
UPAYA PENCEGAHAN STUNTING MELALUI EDUKASI PRODUK OLAHAN IKAN KEPADA IBU PKK KECAMATAN TAHUNA Tanod, Wendy Alexander; Rieuwpassa, Frets Jonas; Mahihody, Astri Juwita; Sambeka, Yana; Cahyono, Eko; Ansar, Novalina Maya Sari; Kasaluhe, Meityn Disye; Ijong, Frans Gruber; Karimela, Ely John; Makisake, Nadia Arlina; Somba, Vira Valentine; Biahimo, Syahputra
Jurnal Pengabdian Pendidikan Masyarakat (JPPM) Vol 6 No 1 (2025): Jurnal Pengabdian Pendidikan Masyarakat (JPPM), Vol 6 No 1 (Maret 2025)
Publisher : LPPM UNIVERSITAS MUHAMMADIYAH MUARA BUNGO

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.52060/jppm.v6i1.2779

Abstract

This community partnership empowerment activity empowers mothers of the Family Welfare Development Team (TP. PKK) in Tahuna District, Sangihe Islands Regency, North Sulawesi through education on diversifying processed fish products to prevent stunting. This activity was carried out in September-October 2024 in three villages focused on handling stunting: Soataloara 1, Mahena, and Santiago, Tahuna District. The purpose of this empowerment activity is to empower the TP. PKK in diversifying processed fish products to help prevent stunting in the Sangihe Islands Regency. The methods used include hearings, initial observations, determining priority programs, facilitation and assistance, and monitoring and evaluation. Activities consisted of counseling on fish nutrition and technical guidance on making processed fish products such as meatballs, nuggets, and kaki naga. The evaluation was conducted through pre and post-tests to measure the improvement of participants' knowledge. The results showed a significant increase in the knowledge and skills of TP. PKK mothers. The average knowledge score on fish nutrition increased from 57% to 87%. As for the technical guidance on processed fish products, the skills improvement was seen in the making of meatballs (64% to 90%), nuggets (62% to 86%), and dragon feet (66% to 94%). This community partnership empowerment activity succeeded in increasing the understanding of PKK women in the Tahuna Sub-district about the importance of fish consumption in preventing stunting and improving the skills of TP. PKK mothers in processing fish into products with high nutritional value.
Pemberdayaan Ibu PKK melalui Diversifikasi Pempek Ikan Layang Berbasis Good Manufacturing Practice dan Sanitation Standard OperatingProcedure Cahyono, Eko; Wodi, Stevy Imelda Murniati; Sambeka, Yana; Rieuwpassa, Frets Jonas; Ansar, Novalina Maya Sari; Tanod, Wendy Alexander; Tengkue, Fiorenza Puspa Indah; Tampi, Regina Romalya Meilan
Amal Ilmiah: Jurnal Pengabdian Kepada Masyarakat Vol. 6 No. 2 (2025): Edisi Mei 2025
Publisher : FKIP Universitas Halu Oleo

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.36709/amalilmiah.v6i2.396

Abstract

Preferensi masyarakat yang meningkat terhadap diversifikasi produk perikanan membuka peluang pasar bagi produk olahan ikan, sebagai salah satu faktor pengembangan UMKM untuk meningkatkan ekonomi masyarakat. Program pengabdian ini bertujuan untuk memberikan pelatihan dan pendampingan kepada kelompok Ibu PKK Kampung Kuma dalam mendiversifikasi produk perikanan, penerapan Good Manufacturing Practice (GMP), Standard Sanitation Operational Procedure (SSOP), dan kelayakan usaha. Metode pelaksanaan pada kegiatan ini terdiri dari beberapa tahapan: survei untuk menentukan lokasi, transfer inovasi secara bertahap, pemberian materi melalui simulasi diversifikasi produk dan pelatihan pemasaran. Hasil dari kegiatan pengabdian masyarakat ini berfokus pada transfer teknologi diversifikasi ikan layang menjadi produk pempek ikan. Analisis usaha menunjukkan bahwa dengan nilai B/C ratio yang lebih dari 1 artinya usaha ini layak secara finansial. Keberhasilan kegiatan ini terlihat dari peningkatan kemampuan peserta setelah pelatihan dari 33,33% menjadi 86,66%. Kesimpulan dari kegiatan pemberdayaan masyarakat ini menunjukkan keberhasilan dalam meningkatkan kapasitas ibu PKK Kampung Kuma dalam produksi pempek ikan layang berbasis GMP dan SSOP. Analisis usaha memberikan pemahaman kepada mitra, bahwa kelayakan UMKM produk pempek ikan layang ini dapat menghasilkan laba bersih yang menguntungkan.
PENGARUH METODE PENGGARAMAN BASAH TERHADAP NILAI SENSORI IKAN LAYANG ASIN (Decapterus sp.) Rieuwpassa, Frets Jonas; Lawendatu, Angryani Enjel; Mandeno, Jefri Anthonius; Tanod, Wendy Alexander; Cahyono, Eko; Ansar, Novalina Maya Sari; Sambeka, Yana; Tomasoa, Aprelia Martina
JURNAL PERIKANAN TROPIS Vol 12, No 1 (2025)
Publisher : Universitas Teuku Umar

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.35308/jpt.v12i1.11570

Abstract

The potential of flying fish (Decapterus sp.) in Sangihe is relatively abundant, yet the catch frequently cannot be absorbed by the market in the peak season. One alternative to overcome the excess catch is to process it into salted fish products. The quality of salted fish is highly dependent on salt concentration and soaking time. This study aimed to observe the effect of soaking time on the sensory quality of flying salted fish. This research was an experimental research with two treatments for the duration of soaking in salt solution with each treatment repeated three times. The research stages included making salted fish using 20% salt concentration with 12 and 24 hours of soaking. Furthermore, the sensory assessment was carried out (SNI no. 8273: 2016) with the parameters of appearance, taste, odor, and texture. Storage was carried out for 30 days at room temperature before being tested for the presence of mold. The results showed that the treatment of soaking time only had a real influence on the sensory value of the appearance of flying salted fish. In contrast, the soaking time did not affect the sensory values of odor (8,47±0,6-8,53±0,4), taste (8,33±0,9-8,40±0,6), and texture (7,87±0,7-7,93±1,0). After being stored for 30 days at room temperature, it was seen that mold had grown on flying salted fish. The study results showed that the 12-hour soaking time had a better value than the 24-hour soaking time.
Edukasi Penerapan EDUKASI PENERAPAN SISTEM RANTAI DINGIN PADA NELAYAN DAN PEKERJA MITRA DI PELABUHAN PERIKANAN PERINTIS DAGHO, KEPULAUAN SANGIHE, SULAWESI UTARA Tanod, Wendy; Ansar, Novalina Maya Sari; Rieuwpassa, Frets Jonas; Palawe, Jaka Frianto Putra; Cahyono, Eko
Jurnal Ilmiah Tatengkorang Vol 8 No 1 (2024): Jurnal Ilmiah Tatengkorang
Publisher : Pusat Penelitian dan Pengabdian Kepada Masyarakat, Politeknik Negeri Nusa Utara

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.54484/tkrg.v8i1.546

Abstract

Kampung Dagho merupakan sentra Perikanan di Kabupaten Kepulauan Sangihe. Kampung Dagho terdapat sarana dan prasarana berupa Pelabuhan Perikanan Perintis dan dua perusahaan penanganan ikan beku, yakni PT. Jassendo Santosa Mandiri dan PT. Perikanan Indonesia Persero. Hasil survei awal diperoleh informasi masih terbatasnya pengetahuan dan keterampilan nelayan dan pekerja di Pelabuhan Perikanan Perintis dalam penanganan ikan melalui sistem rantai dingin. Kegiatan Program Kemitraan Masyarakat Stimulus ini bertujuan untuk memberikan penyuluhan tentang pentingnya penerapan sistem rantai dingin pada ikan hasil tangkapan masyarakat nelayan dan pekerja di Pelabuhan Perikanan Perintis Dagho. Metode pelaksanaan Program Kemitraan Masyarakat Stimulus meliputi penjajakan, identifikasi masalah, penetapan pelaksanaan kegiatan, penyusunan materi dan instrument penyuluhan, pemberian materi, dan evaluasi. Materi penyuluhan yang diberikan, yaitu 1. faktor penyebab kemunduran mutu ikan; 2. penerapan sistem rantai dingin pada penanganan ikan, dan 3. pentingnya sanitasi dan hygiene pada proses penanganan ikan. Dari kegiatan Program Kemitraan Masyarakat Stimulus ini telah berhasil meningkatkan pengetahuan nelayan dan pekerja tentang pentingnya penerapan sistem rantai dingin dan sanitasi hygiene selama proses distribusi ikan dari kapal hingga pada saat proses produksi. Sinergitas antara pemerintah, perusahaan dan akademisi sangat penting dilakukan secara berkala untuk mengedukasi para nelayan dan pekerja khususnya yang beraktivitas di Pelabuhan Perikanan Perintis Dahgo. Dagho village is a fishery center in the Sangihe Islands Regency. Dagho village has facilities and infrastructure from a Pioneer Fishery Port and two frozen fish handling companies, namely PT. Jassendo Santosa Mandiri and PT. Perikanan Indonesia Persero. The initial survey results revealed that the knowledge and skills of fishermen and workers at the Pioneer Fishery Port still needed to be improved in handling fish through the cold chain system. This Stimulus Community Partnership Program activity aims to provide information on the importance of applying the cold chain system to fish caught by fishing communities and Dagho Pioneer Fishing Port workers. Implementing the Stimulus Community Partnership Program includes assessment, problem identification, determination of activity implementation, preparation of extension materials and instruments, provision of materials, and evaluation. The counseling materials provided were: 1. the factors causing the deterioration of fish quality; 2. application of cold chain systems in fish handling; and 3. the importance of sanitation and hygiene in the fish handling process. The activities of the Stimulus Community Partnership Program have increased fishermen's and workers' knowledge about the importance of implementing a cold chain system and sanitation hygiene during the fish distribution from ships to the production process. The synergy between the government, companies, and academics must be carried out regularly to educate fishermen and workers, especially those active in the Dahgo Pioneer Fishing Port.
Co-Authors AA Sudharmawan, AA Alfira Opinri Sehangunaung Anggraini Barlian Aprelia M. Tomasoa Aprelia M. Tomasoa Aprelia Martina Tomasoa Aprelia Martina Tomasoa, Aprelia Martina Aprelia Tomasoa Azhari, Deidy Balansa, Walter Biahimo, Syahputra Cahyani, Reni Tri Cahyono, Eko Christian A. Manansang Devitha Cindy Lasaru Dewanto, Didit Kustantio Dewanto, Didit Kustantio Edward Irsan L. Ferre EKO CAHYONO Eko Cahyono, Eko Ely John Karimela Eunike Irene Kumaseh Fitria F. Lungary Frans Gruber Ijong Herjumes Aatjin Ida Ayu Putu Sri Widnyani If'all, If'all Imra Imra Indra Wibowo Irna Yanti Dalawir Jaka F.P. Palawe Jaka Frianto Putra Palawe Jefri Antonius Mandeno Jimi Palawe Joko Santoso Kendek, Tasya Jenifel Kundiman, Gita Artika Putri La Ode Huli Laode Muhamad Hazairin Nadia Lawendatu, Angryani Enjel Maalua, Sheyla Mahihody, Astri Mahihody, Astri Juwita Makisake, Nadia Arlina Maliode, Yunita Isabela Mandeno, Jefri Anthonius Mandiangan, Meyrijean Marnens Ch. Karaeng Marnens Christianto Karaeng Meityn Disye Kasaluhe Melongkade, Regina Juliana Natalia Prodiana Setiawati Novalina M. Ansar Novalina Maya Sari Ansar Novelia Tatinting Nurhikma Nurhikma Obyn I. Pumpente Obyn Imhart Pumpente Revolson Mege Rolando Pangumpia Rorong, Miranda Serfiyanti Sirih Siegfried Berhimpon Somba, Vira Valentine Stefiani Nofrida Asare Stevy I. M. Wodi Stevy Imelda Murniati Wodi Steward I. Makawekes Tampany, Ighen Tampi, Regina Romalya Meilan Tanod, Wendy Tengkue, Fiorenza Puspa Indah Tengkue, Florenza Puspa Indah Trivonia Kerol Talete Usy N. Manurung Walter Balansa Walter Balansa Waode Nilda Arifiana Effendy Wendy Alexander Tanod Wini - Trilaksani Wodi, Stevy Imelda Murniati Wulandari Wulandari Yana Sambeka