Article Per Year (5 Year)
p-Index From 2021 - 2026
2.693
P-Index
This Author published in this journals
All Journal HAYATI Journal of Biosciences Jurnal Ilmu Pertanian Indonesia Jurnal Fitopatologi Indonesia Jurnal Hortikultura Indonesia (JHI) Microbiology Indonesia Agrovigor: Jurnal Agroekoteknologi Jurnal Hortikultura Jurnal Penelitian Tanaman Industri Buletin Palawija AGRIVITA, Journal of Agricultural Science Jurnal Perlindungan Tanaman Indonesia JURNAL HAMA DAN PENYAKIT TUMBUHAN TROPIKA Jurnal Penelitian Pertanian Tanaman Pangan Jurnal Entomologi Indonesia Jurnal Penelitian Tanaman Industri (Littri) Buletin Palawija
TRI ASMIRA DAMAYANTI
Departemen Proteksi Tanaman, Fakultas Pertanian IPB, Jln. Kamper, Kampus IPB Dramaga, Bogor, Jawa Barat, Indonesia, 16680
Agriculture, Biological Sciences & Forestry Biochemistry, Genetics & Molecular Biology Earth & Planetary Sciences Immunology & microbiology Industrial & Manufacturing Engineering Medicine & Pharmacology

Published : 67 Documents Claim Missing Document
Claim Missing Document
Check
Articles

Found 4 Documents
Search
Journal : HAYATI Journal of Biosciences

 1 
Severe Outbreak of a Yellow Mosaic Disease on the Yard Long Bean in Bogor, West Java TRI ASMIRA DAMAYANTI; OLUFEMI JOSEPH ALABI; RAYAPATI ADIKESAVULU NAIDU; AUNU RAUF
HAYATI Journal of Biosciences Vol. 16 No. 2 (2009): June 2009
Publisher : Bogor Agricultural University, Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (173.223 KB) | DOI: 10.4308/hjb.16.2.78

Abstract

During 2008 crop season, an outbreak of severe yellow mosaic disease on yard long bean (Vigna unguiculata subsp. Sesquipedalis) occurred in several farmers’ fields in West Java. Yard long bean var. Parade inoculated manually with extracts from symptomatic leaves showed the symptoms indicating the presence of virus. Symptomatic leaf samples tested positive in enzyme linked immunosorbent assay (ELISA) with antibodies to group specific Potyvirus and Cucumber mosaic virus (CMV). Total RNA derived from symptomatic leaves was subjected to reverse transcription-polymerase chain reaction (RT-PCR) using primers specific to the cylindrical inclusion (CI) protein of potyviruses and CMV coat protein (CP) specific primers. Pair wise comparison of sequences obtained from cloned RT-PCR products with corresponding nucleotide sequences in the GenBank confirmed the presence of Bean common mosaic virus strain Blackeye (BCMV-BlC) and CMV in the symptomatic beans. Sequences of BCMV and CMV isolates from the beans showed maximum nucleotide sequence identities (92-97%) and (90%), respectively with BCMV-BIC and CMV isolates from Taiwan. Each virus isolate also clustered closely with corresponding isolates from Taiwan in a phylogenetic analyses. These results provide first evidence of the occurrence of multiple infection of BCMV-BIC and CMV in the yard long been from Bogor, West Java. Key words: yard long bean, BCMV-BIC, CMV, Bogor Indonesia
Utilization of Root-Colonizing Bacteria to Protect Hot-Pepper Against Tobacco Mosaic Tobamovirus TRI ASMIRA DAMAYANTI; HENDRA PARDEDE; NISA RACHMANIA MUBARIK
HAYATI Journal of Biosciences Vol. 14 No. 3 (2007): September 2007
Publisher : Bogor Agricultural University, Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (56.048 KB) | DOI: 10.4308/hjb.14.3.105

Abstract

Tobacco Mosaic Tobamovirus (TMV) is one of many important viruses infecting Solanaceous plants including hot pepper in Indonesia. To accomplish and improve the effectiveness of virus management, we used root-colonizing bacteria (rhizobacteria) isolated from healthy hot pepper. Eight rhizobacteria isolates were selected and their capacity in enhancing plant growth and inducing systemic resistance (ISR) against TMV in greenhouse trials were evaluated. The rhizobacteria was applied as seed treatment and soil drench. Bacterized-seedling showed a better growth vigor, fitness and a milder symptom than non-bacterized control plants. The protective effect of rhizobacteria was more pronounced after challenging inoculation by TMV, especially for plants treated by isolates I-6, I-16, and I-35. However, TMV accumulation was slightly affected by bacterial treatment. The rhizobacteria might improved ISR by increasing peroxidase enzyme activity but this depends on the species. Based on whole results, isolate I-35 was the potential plant growth promotion rhizobacteria (PGPR). The I-35 was identified as Bacillus cereus based on morphological characteristics and nucleotide sequences of 16S r-RNA. Key words: root-colonizing bacteria, TMV, ISR
Identification and Molecular Characterization of Odontoglosum Ringspot Virus (ORSV) from Bogor, Indonesia IRWAN LAKANI; GEDE SUASTIKA; NURHAJATI MATTJIK; TRI ASMIRA DAMAYANTI
HAYATI Journal of Biosciences Vol. 17 No. 2 (2010): June 2010
Publisher : Bogor Agricultural University, Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (130.571 KB) | DOI: 10.4308/hjb.17.2.101

Abstract

Recently, field surveys were conducted in several orchid nurseries in Bogor (West Java), Magelang (Central Java) and Malang (East Java). We found that most of the commercially orchids sampled from Bogor was infected by virus-like disease. The symptoms depend on the orchid species. Thus, the symptoms varied such as mottle, mosaic and necrotic flecks either on the leaves or on the flowers. Similiar symptoms were not found in samples obtained from Central Java and East Java. A Tobamo-like virus was inferred to be possible cause of the viral disease-like symptoms. Serological test of the samples by ELISA showed positive against Odontoglosum Ringspot Virus (ORSV) antibody and was negative against Cymbidium Mosaic Virus (CyMV) antibody. Total RNA was extracted from symptomatic plants and RT-PCR was carried out by using a pair of coat protein (CP) gene primer of ORSV.  It was successfully amplified a 500 bp of DNA fragment and it was directly sequenced. The nucleotide sequence of CP gene had confirmed the identity of ORSV. Phylogenetic analysis based on the CP nucleotide sequences were grouped into only one major cluster.  The ORSV isolate from Bogor (Sd 21) and the other isolates were clustered in the same group and had highest nucleotide homology (99%). These results provide first evidence of ORSV infecting orchids in Bogor, Indonesia.
Expression of Recombinant Sugarcane Streak Mosaic Virus Coat Protein Gene in Escherichia coli Hamdayanty Hamdayanty; Sri Hendrastuti Hidayat; Tri Asmira Damayanti
HAYATI Journal of Biosciences Vol. 23 No. 3 (2016): July 2016
Publisher : Bogor Agricultural University, Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (1138.385 KB) | DOI: 10.4308/hjb.23.3.111

Abstract

Sugarcane streak mosaic virus (SCSMV) is an important virus causing mosaic disease in sugarcane and transmitted through the cutting cane. Commercial antiserum to detect SCSMV and to monitor the disease development is not available. The research was conducted to produce antigen of SCSMV coat protein (SCSMV-CP) through overexpressing it on bacterial expression which will be used for antiserum production. SCSMV-CP was amplified using specific primers for CP gene containing BamHI and HindIII restriction enzyme sites and cloned into pTZ57R/T. Subsequently, the SCSMV-CP was subcloned into pET28a and transformed on Escherichia coli BL21(DE3) and Rosetta-gami(DE3)pLysS. The concentration of isopropyl β-d-thiogalactopyranoside (IPTG), incubation temperature, and bacterial harvesting time after IPTG induction were optimized. SCSMV-CP gene was successfully amplified with size ±855 bp, subcloned into vector expression, and expressed in insoluble fraction either in both bacterial host. Optimal protein expression of SCSMV-CP recombinant was obtained at 25°C with IPTG concentration 0.25–1.00 mM and harvested at 9–12 hours after IPTG induction in E. coliBL21(DE3), and at 30°C with IPTG concentration 0.25–1.00 mM and harvested 3–12 hours after IPTG induction in E. coli Rosetta(DE3)pLysS. SDS-PAGE analysis showed that protein size of SCSMV-CP recombinant was ±35.4 kDa.
Co-Authors . Giyanto . Haryanto Abdjad Asih Nawangsih Ade Syahputra Akhiruddin Akhiruddin Anastasya Hondo Anastasya Hondo Annisa Nur Imamah Cahyati, Iwe Dewa Gede Wiryangga Selangga DEWI SARTIAMI Dita Megasari Dwi Subekti Efendi, Darda Efi Toding Tondok Endah Muliarti Erniawati Diningsih Erniawati Diningsih Erniawati Diningsih Erniawati Diningsih Farida, Naimatul Gede Suastika Gede Suastika Gede Suastika GEDE SUASTIKA Gede Suastika Gede Suastika GEDE SUASTIKA GEDE SUASTIKA Gede Suastika Giyanto Giyanto Giyanto Giyanto Gusti Ngurah Alit Susanta Wirya Haerani Haerani Hagia Sophia Khairani Hamdayanty Hamdayanty HENDRA PARDEDE I Dewa Nyoman Nyana Ida Parida Imamah, Annisa Nur Imaniasita, Vidya Irwan Lakani IRWAN LAKANI Islami, Nisa Fadhila Januarsih, Vera Jati Adiputra Kartika Catur Damaiyanti Ketut Ayu Yuliadhi Kikin H Mutaqin L Pebriyeni Ladja, Fausiah T. Laras Anjarsari Lilik Koesmihartono Putra Listihani, Listihani Lulu Kurnianingsih Martha Theresia Panjaitan Melinda . Mhd Rifqi Abdillah Miftakhurohmah . Miftakhurohmah Miftakhurohmah MIFTAKHUROHMAH MIFTAKHUROHMAH MIFTAKHUROHMAH MIFTAKHUROHMAH, MIFTAKHUROHMAH Mimi Sutrawati Muhammad Fikri Hafizh Munif Ghulamahdi N Mattjik Ni Putu Pandawani Niken Nur Kasim, Niken Nur Niky Elfa Amanatillah NISA RACHMANIA MUBARIK Nur Unsyah Laili Nurfadillah NURHAJATI MATTJIK OLUFEMI JOSEPH ALABI Pratiwi, Rizky Nomi Primandani, Dhika Kintania Purnamawati, Iis Purwono Purwono Putri, Maharani Mustika R Kartika RAUF, AUNU RAYAPATI ADIKESAVULU NAIDU Rita Noveriza Rita Noveriza Rizki Haerunisa S Susanto Slamet Susanto Sofranita Syifa Fitriyati SRI HENDRASTUTI HIDAYAT Sugeng Santoso Sugeng Santoso Supramana Suryo Wiyono Syaiful Khoiri TRISNA AGUNG PHABIOLA Wahyudin, Denih Wieke Mei Dina Yunita Fauziah Rahim Yunita Fauziah Rahim Yusmani Prayogo Zulfi, Fahma Zakiya