TRI ASMIRA DAMAYANTI
Departemen Proteksi Tanaman, Fakultas Pertanian IPB, Jln. Kamper, Kampus IPB Dramaga, Bogor, Jawa Barat, Indonesia, 16680

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Journal : Microbiology Indonesia

Genetic Diversity of Cucumber Mosaic Virus Strain Soybean from Several Areas TRI ASMIRA DAMAYANTI; SURYO WIYONO
Microbiology Indonesia Vol. 9 No. 1 (2015): March 2015
Publisher : Indonesian Society for microbiology

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (872.412 KB) | DOI: 10.5454/mi.9.1.6

Abstract

Cucumber mosaic virus strain soybean (CMV-S) is one of economically important virus infecting soybean in Indonesia. However, it is very few information related with the CMV-S Indonesia isolates. Thus, the aim of present work is to detect and identify CMV-S isolates from different origin. Leaf samples collected from six different soybean fields in Java and North Sumatera. Molecular detection was carried out by reverse transcription polymerase chain reaction (RT-PCR) using specific primer of the coat protein (CP) gene and DNA sequencing. RT-PCR were successfully amplified the entire CP gene size ~657 bp from all samples and encoded 218 amino acids. Analysis of nucleotide and amino acid sequences showed high homology among isolates ranging from 99.5% and 100%. However isolate from Sindang Laut either nucleotides or amino acids showed lower homology to that of five isolates ranging from 89.4%-89.6% and 91.7%, respectively. The differences between CMV-S with other non legume strain found in six amino acid positions in the CP gene, suggesting the differences might related with host specificity. Phylogenetic analysis of amino acid of six isolates to other CMV strain showed that the five CMV-S isolates were in the same cluster, while Sindang Laut isolate was in different cluster together with the non-legume CMV from India. It is indicating the present of genetic variability among CMV-S isolates in Java.
Co-Authors . Giyanto . Haryanto Abdjad Asih Nawangsih Ade Syahputra Akhiruddin Akhiruddin Anastasya Hondo Anastasya Hondo Annisa Nur Imamah Cahyati, Iwe Dewa Gede Wiryangga Selangga DEWI SARTIAMI Dita Megasari Dwi Subekti Efendi, Darda Efi Toding Tondok Endah Muliarti Erniawati Diningsih Erniawati Diningsih Erniawati Diningsih Erniawati Diningsih Farida, Naimatul Gede Suastika Gede Suastika GEDE SUASTIKA Gede Suastika Gede Suastika GEDE SUASTIKA GEDE SUASTIKA Gede Suastika Gede Suastika Giyanto Giyanto Giyanto Giyanto Gusti Ngurah Alit Susanta Wirya Haerani Haerani Hagia Sophia Khairani Hamdayanty Hamdayanty HENDRA PARDEDE I Dewa Nyoman Nyana Ida Parida Imamah, Annisa Nur Imaniasita, Vidya Irwan Lakani IRWAN LAKANI Islami, Nisa Fadhila Januarsih, Vera Jati Adiputra Kartika Catur Damaiyanti Ketut Ayu Yuliadhi Kikin H Mutaqin L Pebriyeni Ladja, Fausiah T. Laras Anjarsari Lilik Koesmihartono Putra Listihani, Listihani Lulu Kurnianingsih Martha Theresia Panjaitan Melinda . Mhd Rifqi Abdillah Miftakhurohmah . MIFTAKHUROHMAH MIFTAKHUROHMAH Miftakhurohmah Miftakhurohmah MIFTAKHUROHMAH MIFTAKHUROHMAH, MIFTAKHUROHMAH Mimi Sutrawati Muhammad Fikri Hafizh Munif Ghulamahdi N Mattjik Ni Putu Pandawani Niken Nur Kasim, Niken Nur Niky Elfa Amanatillah NISA RACHMANIA MUBARIK Nur Unsyah Laili Nurfadillah NURHAJATI MATTJIK OLUFEMI JOSEPH ALABI Pratiwi, Rizky Nomi Primandani, Dhika Kintania Purnamawati, Iis Purwono Purwono Putri, Maharani Mustika R Kartika RAUF, AUNU RAYAPATI ADIKESAVULU NAIDU Rita Noveriza Rita Noveriza Rizki Haerunisa S Susanto Slamet Susanto Sofranita Syifa Fitriyati SRI HENDRASTUTI HIDAYAT Sugeng Santoso Sugeng Santoso Supramana Suryo Wiyono Syaiful Khoiri TRISNA AGUNG PHABIOLA Wahyudin, Denih Wieke Mei Dina Yunita Fauziah Rahim Yunita Fauziah Rahim Yusmani Prayogo Zulfi, Fahma Zakiya