Article Per Year (5 Year)
p-Index From 2020 - 2025
2.957
P-Index
This Author published in this journals
All Journal Jurnal Hortikultura Indonesia (JHI) METAMORFOSA Journal of Biological Sciences Buletin Udayana Mengabdi Jurnal Biologi Udayana SIMBIOSIS Biota Jurnal Mikologi Indonesia Agritrop : Jurnal Ilmu-Ilmu Pertanian (Journal of Agricultural Science) BIO-CONS: Jurnal Biologi dan Konservasi BIO-CONS: Jurnal Biologi dan Konservasi BIOSAPPHIRE: Jurnal Biologi dan Diversitas
Made Ria Defiani
Program Studi Biologi, Fakultas Matematika Dan Ilmu Pengetahuan Alam, Universitas Udayana
Agriculture, Biological Sciences & Forestry Biochemistry, Genetics & Molecular Biology Chemical Engineering, Chemistry & Bioengineering Decision Sciences, Operations Research & Management Education Energy Environmental Science Immunology & microbiology Materials Science & Nanotechnology Medicine & Pharmacology Other

Published : 32 Documents Claim Missing Document
Claim Missing Document
Check
Articles

Found 7 Documents
Search
Journal : METAMORFOSA Journal of Biological Sciences

 1 
PERTUMBUHAN ANGGREK Vanda helvola PADA MEDIA YANG DIPERKAYA JUS TOMAT Yully Muharyati; Made Ria Defiani; Ni Putu Adriani Astiti
Metamorfosa: Journal of Biological Sciences Vol 2 No 2 (2015)
Publisher : Prodi Magister Ilmu Biologi, Fakultas MIPA, Universitas Udayana

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.24843/metamorfosa.2015.v02.i02.p03

Abstract

Vanda is one of the most cultivated orchid species.  In nature, Vanda is endangered because it was exploited in large quantities. Orchid genetic viability can be maintained by embryo culture. The study aimed to investigate the growth of Vanda helvola seeds during 12 weeks after cultured. Media Murashige and Skoog (MS) added with tomato juice 100 g/L was used as culture medium. The results showed that embryo could grow on MS medium on stadium 6th. Protocorm started to form at 9 weeks after cultured. At 12 weeks, percentage of protocorm formed reach almost 24.1%. Tomato juice improved the growth of protocorm of  V.helvola. Key words: Medium, protocorm, Vanda helvola
Induksi Perkecambahan In Vitro Jeruk Hasil Fusi Protoplas Pada Medium Dengan Penambahan Asam Giberelat A A Ayu Laksmi Damarnegari; Ida Ayu Astarini; Made Ria Defiani; Mia Kosmiatin
Metamorfosa: Journal of Biological Sciences Vol 7 No 2 (2020)
Publisher : Prodi Magister Ilmu Biologi, Fakultas MIPA, Universitas Udayana

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.24843/metamorfosa.2020.v07.i02.p10

Abstract

Plant breeding is an effort to develop plants by manipulating the genetic system of plants to get new superior cultivars. Plant breeding often produces incompatible seeds, so a rescue process is needed to reduce the risk of embryo death. The addition of gibberellic acid could break the seed dormancy for seed that hard to germinate. The purpose of this study was to find out the best concentration of gibberellic acid for germination of citrus globular embryos from protoplast fusion. Research was conducted at the Biology and Cell Biology Research Group In Vitro Culture Laboratory, Indonesian Center for Agricultural Biotechnology and Genetic Resources Research and Development (BB BIOGEN), Agricultural Research and Development Agency, Ministry of Agriculture, Bogor. The study used Completely Randomized Design (CRD) with concentration 0, 1, 2 and 3 ppm of gibberellic acid as treatment, each consists of 7 replicates. This study used globular embryos measuring ± 3 mm of inbred FS 29 and FS 89 from protoplast fusion between Siam Simadu and Mandarin Satsuma citrus. Observations were made on the percentage of germinated embryos, time of germination, the number of the complete and incomplete shoot (abnormal, only part of the roots or buds that developed). Data were analyzed using Crosstab analysis test with IBM SPSS Statistics 22 software. Result showed that addition of gibberellic acid induce faster germination time and increase seedling percentage. The best germination medium for citrus FS 29 was VMW with 2 ppm and 3 ppm gibberellic acid with the seedling percentage around 85,7% in 7 days, while the best germination medium for citrus FS 89 was VMW with 1 ppm gibberellic acid with 100% seedling embryos in 8,5 days. VMW medium added with 1 ppm, 2 ppm and 3 ppm gibberellic acid for citrus FS29 and VMW medium added with 2 ppm and 3 ppm for citrus FS89 were good to get the complete sprouts with a succes rate of 100%. Keyword: Citrus sp. gibberellic acid, incompatible, in vitro, embryo germination
Aplikasi Spora Endomikoriza, Kompos Dan Trichoderma Spp. Meningkatkan Pertumbuhan Bibit Kakao (Theobroma cacao L.) Tadzkia Hanifah Akbar; Meitini Wahyuni Proborini; Made Ria Defiani
Metamorfosa: Journal of Biological Sciences Vol 9 No 1 (2022)
Publisher : Prodi Magister Ilmu Biologi, Fakultas MIPA, Universitas Udayana

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.24843/metamorfosa.2022.v09.i01.p05

Abstract

Pembibitan kakao (Theobroma cacao L.) merupakan salah satu upaya untuk mempertahankan agroforestri dan menjaga fungsi hutan. Penelitian bertujuan menganalisis kombinasi antara jamur endomikoriza, kompos, dan Trichoderma spp. terhadap pertumbuhan bibit kakao serta menentukan konsentrasi yang terbaik untuk meningkatkan pertumbuhan bibit kakao. Penelitian dilakukan di laboratorium taksonomi tumbuhan (mikologi) dan green house Persemaian Permanen Suwung, Badan Pengelolaan Daerah Aliran Sungai dan Hutan Lindung Hutan Anyar. Perlakuan disusun secara RAK (Rancangan Acak Kelompok) dengan 5 perlakuan yang terdiri dari 5 ulangan dan 3 unit tanaman untuk setiap ulangan. Perlakuan pertama menggunakan tanah steril (kontrol negatif), perlakuan kedua dengan tanah steril + kompos 10 g + Trichoderma spp 10 mL (kontrol positif), perlakuan ketiga dengan tanah steril dan inokulasi endomikoriza Glomus sp 100 butir + kompos 10 gram + Trichoderma spp. 10 mL, perlakuan keempat dengan tanah steril dan inokulasi endomikoriza Glomus sp 150 butir + kompos 10 g + Trichoderma spp. 10 mL, dan perlakuan kelima dengan tanah steril dan inokulasi endomikoriza Glomus sp 200 butir + kompos 10 g+ Trichoderma spp. 10 mL. Hasil penelitian menunjukkan bahwa perlakuan kelima (200 spora endomikoriza, 10 mL Trichoderma spp., dan 10 g kompos) merupakan kombinasi terbaik untuk pertumbuhan bibit kakao dilihat dari nilai parameter tinggi tanaman sebesar 29,03 cm, berat kering tanaman 3,55 g, berat kering akar 1,83 g, dan persentase kolonisasi endomikoriza sebesar 6,33 %. Kata kunci: Green house, Glomus sp., agroforestri, persentase kolonisasi
INDUKSI PERTUMBUHAN TUNAS DARI EKSPLAN ANGGREK Dendrobium Heterocarpum Lindl. DENGAN PEMBERIAN HORMON ZEATIN DAN NAA Ni Luh Putu Kayika Febryanti; Made Ria Defiani; Ida Ayu Astarini
Metamorfosa: Journal of Biological Sciences Vol 4 No 1 (2017)
Publisher : Prodi Magister Ilmu Biologi, Fakultas MIPA, Universitas Udayana

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.24843/metamorfosa.2017.v04.i01.p07

Abstract

Dendrobium heterocarpum Lindl. is a native orchid in Indonesia. Its population continue to decline due to unsustainable exploitation. One solution to increase its population is via in vitro technique propagation. This research aimed to determine effect of hormone addition (Zeatin and NAA) to induce shoot multiplication of D. heterocarpum Lindl. grown in Murashige and Skoog (MS) basic medium. The research was conducted in Tissue Culture Laboratory, Department of Biology, Faculty of Mathematics and Science, Udayana University from November 2015 until March 2016. The experiment employed Completely Randomized Design (CRD) with 20 combinations of treatments, with three replicates each treatment. The treatment werer addition of Zeatin and NAA, with five concentrations of Zeatin (0, 0.1, 0,5, sweet corn and hybrids corn as the natural Zeatin); four NAA concentrations (0, 0.05, 0.1 and 0.5). Variable recorded include such as the number of shoots, roots and explant’s vigor. The quantitative data which include number of leaves and shoots after 12 weeks, were analyzed using Analysis of Variance (ANOVA); and if significantly different, tested further using Duncan Multiple Range Test (DMRT) at 5% level. Z5N3 medium revealed significantly different on amount of leaves and shoot height and also the best medium on amount of roots. Best hormone combination that can induce multiplication of new shoots are medium Z4N3 and Z5N1.
Perbanyakan Klonal Galur Jeruk Siam Kintamani (Citrus nobilis Lour) Hasil Iradiasi Sinar Gamma Puji Wahyu Lestari; Made Ria Defiani; Mia Kosmiatin
Metamorfosa: Journal of Biological Sciences Vol 9 No 1 (2022)
Publisher : Prodi Magister Ilmu Biologi, Fakultas MIPA, Universitas Udayana

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.24843/metamorfosa.2022.v09.i01.p20

Abstract

Jeruk siam Kintamani disukai oleh konsumen karena memiliki rasa manis, harum, dan daging buahnya lunak. Namun jeruk ini memiliki biji relatif banyak dan warna kulit kurang menarik sehingga kalah bersaing dengan jeruk yang diproduksi oleh negara lain. Alternatif untuk meningkatkan kualitas buah jeruk adalah melalui induksi mutasi in vitro menggunakan sinar gamma. Tunas putative mutan jeruk siam diperbanyak secara in vitro sehingga diperoleh duplikat dari masing-masing mutan. Penggunaan jenis media dan zat pengatur tumbuh pada konsentrasi yang tepat merupakan salah satu kunci keberhasilan dalam kultur jaringan. Tujuan penelitian ini adalah untuk memacu multiplikasi tunas dari eksplan buku. Bahan yang digunakan yaitu plantlet jeruk siam Kintamani hasil iradiasi sinar gamma dengan dosis 0, 4,5, 5, dan 5,5 Gy. Penelitian multiplikasi tunas secara in vitro menggunakan eksplan buku dengan perlakuan 7 jenis media. Hasil penelitian menunjukkan bahwa pada perlakuan media MS VMW + BAP2 + GA2 mampu memperbanyak jumlah tunas, sedangkan media MS VMW mampu meningkatkan jumlah daun dan perbanyakan jumlah akar. Kata kunci : putative mutan, karakterisasi, multiplikasi, mutasi
PERTUMBUHAN VEGETATIF DAN PRODUKTIVITAS BERBAGAI KULTIVAR BROKOLI (Brassica oleracea L. var. italica Plenck.) INTRODUKSI DI DESA BATUR, KECAMATAN KINTAMANI, KABUPATEN BANGLI, BALI Ni Kadek Raleni; Made Ria Defiani; Ida Ayu Astarini
Metamorfosa: Journal of Biological Sciences Vol 2 No 2 (2015)
Publisher : Prodi Magister Ilmu Biologi, Fakultas MIPA, Universitas Udayana

Show Abstract | Download Original | Original Source | Check in Google Scholar

Abstract

Broccoli (Brassica oleracea L. var. italica Plenck.) is a vegetable crop belongs to Brassicaceae family.  Broccoli has high nutrition, high in fiber and contains isotiacyanate that has anticancer activity.  Broccoli market in Indonesia, particularly in modern supermarkets, increases 15-20% per year, however, productivity was still low, therefore effort to increase broccoli production in Indonesia need to be investigated. Field trial was conducted at Batur Village, Kintamani District, Bangli Regency, Bali, to find out cultivars that were adapted in tropical region. Introduced cultivars being trialed were ‘Alborada’,‘Belstar’, ‘Fiesta’, ‘Sarasota’, ‘Bay Meadows’, ‘Castle Dome’, ‘Liutenant’, ‘Iron Man’, ‘Marathon’, ‘Green Gold’, ‘Imperial’, ‘Green Magic’ and ‘Lucky’ as control.  Variable observed were vegetative growth, curding period, and productivity of each cultivar.  This study employed Randomized Completely Block Design with 3 replicates (plots) and 8 plants each plot.  Data were analyzed using ANOVA (Analysis of variance) on Costat program, followed by Duncan’s Multiple Range Test (DMRT) on 1% level.  Results show that each cultivar varied in adaptability in tropical region. ‘Castle Dome’ has the highest productivity, while ‘Fiesta’ was the lowest. Keywords: Brassica, field trial, cultivar
Aktivitas Antibakteri Ekstrak Etanol Daun Ketapang (Terminalia catappa L.) Merah dan Cokelat Terhadap Pertumbuhan Bakteri Staphylococcus aureus Luh Made Ary Somia Vagestini; Retno Kawuri; Made Ria Defiani
Metamorfosa: Journal of Biological Sciences Vol 10 No 1 (2023)
Publisher : Prodi Magister Ilmu Biologi, Fakultas MIPA, Universitas Udayana

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.24843/metamorfosa.2023.v10.i01.p17

Abstract

Staphylococcus aureus is known as bacteria that often cause respiratory tract disease in Indonesia. These bacteria are known to have developed resistance to several antibiotics. This study aims to analyze the comparison of the antibacterial activity of ethanol extracts of red ketapang leaves and brown ketapang leaves on the growth of S.aureus bacteria, determine the MIC of the two extracts, and determine the class of compounds in the extracts. The research method used includes sample extraction using the maceration method with 96% ethanol as solvent. The antibacterial activity test of the extract was carried out at concentrations of 20%, 15%, 10%, and 5% (w/v) with the disc diffusion method on Nutrient Agar media, and qualitative phytochemical tests were carried out. The results showed that the difference in leaf color affected the inhibition of S. aureus bacteria. The best inhibition was obtained at a concentration of 20% (w/v) red ketapang extract with an inhibition zone diameter of 13.67±0.76 mm. The results of the MIC test showed that the MIC value of the red ketapang extract was obtained at a concentration of 5% (w/v) with an inhibitory power of 1.47±0.25 mm and the cocoa bean leaf extract was obtained at a concentration of 10% (w/v) with the diameter of the inhibition zone. 1.73±0.25mm. The phytochemical test of the extracts showed that both extracts contained alkaloids, saponins, flavonoids, tannins, and steroids, but terpenoid compounds were only found in the red ketapang leaf extract. Keywords: secondary metabolites, MIC, resistance, ketapang leaf color.
Co-Authors A A Ayu Laksmi Damarnegari A.A.S. A. Sukmaningsih Anitasari, Septarini Dian Dian Catur Prayantini Dwi Nur Rikhma Sari Eniek Kristiyanti ENIEK KRISWIYANTI Hikmah Putri Wahyu Utami I KETUT MUKSIN I Made Anom Sutrisna Wijaya I Made Saka Wijaya I.M.A.S. Wijaya I.M.S. Wijaya IDA AYU ASTARINI Ida Bagus Gede Darmayasa Ida Bagus Wayan Gunam Lily Windasari Luh Made Ary Somia Vagestini Luh Putu Melandani Luh Putu Wrasiati MADE PHARMAWATI MADE SUSUN PARWANAYONI Meitini Proborini Wahyuni Mia Kosmiatin Ni Kadek Raleni Ni LUH ARPIWI Ni Luh Putu Kayika Febryanti Ni Luh Suriani Ni Putu Adriani Astiti Ni Putu Harta Diani Pande Ni Putu Yuliatiningsih Novita Sari Puji Wahyu Lestari Putu Wina Andriani Lestari Rachel Daniella Dinda Maria Lumban Tobing RANI RACHMAWATI Retno Kawuri Septarini Dian Anitasari Septarini Dian Anitasari Septarini Dian Anitasari Tadzkia Hanifah Akbar Wira Purnama Santi Yully Muharyati