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Journal : Animal Production : Indonesian Journal of Animal Production

The Quantity and Quality of Brahman Cross Cattle Embryo After Injected FSH and PMSG Adriani, Adriani; Rosadi, B; Depison, Depison
ANIMAL PRODUCTION Vol 11, No 2 (2009): May
Publisher : Universitas Jenderal Soedirman, Faculty of Animal Science, Purwokerto-Indonesia

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Abstract

Twenty cattles were used in this experiment to determine the  quantity and  quality of embryo after injected FSH (follicle stimulating hormone ) and  PMSG (pregnant mare serum gonadotrophin) in Brahman Cross Cattle. The experiment was assigned into Completely Randomized Design with 5 treatments and 4 replications. The treatments were T1 = 4 mg of FSH twice a day intra-ovary decreased doses, T2 = 8 mg of FSH twice a day intra-ovary decreased doses, T3 = 300 IU of PMSG single dose intra-ovary, T4 = 600 IU of PMSG single dose intra-ovary, T5 = 40 mg of FSH twice a day intramuscular decreased doses. Trial cattle were oestrus synchronized using 15 mg of PGF2α that gave twice at 11-daily intervals. One day after giving FSH and PMSG was detected the cattle’s oestrus. Washing uterus  was done at day 7 after AI using mixture of PBS, FCS and streptomicyn. Data observed were cow performances, embryo quantity and embryo quality. Results of experiment showed that 19 cattle (95%) responded oestrus synchronized treatment and super ovulation, whereas 1 cattle (5%) did not respond oestrus synchronized treatment and super ovulation. Generally, cattle showed oestrus at 2 – 3 days after giving PGF2α. Eleven cattle (57,90%)  showed oestrus at 2 days after giving PGF2α whereas the others (8 cattle, 42,10%) showed oestrus 3 days giving PGF2α. The treatment of giving FSH and PMSG could increase (P<0,05) embryo. T5 was highest  compared the others ( T1, T2, T3 and T4), while T2 and T4 were higher than T1 and T3. Produced total embryo was 82 with average 4,3 ± 5,67 using FSH and PMSG. 8 embryo (9,76%), 9 embryos (10,90), 20  embryo (24,40%), 16 embryo (19,50%) and  29 embryos (35,40%) were grade A, B, C, D and E respectively. It is concluded that giving of 40 mg  FSH  intramusculer produce the best embryo donor whereas  and giving  of FSH 8 mg intraovari was the best effeciency. (Animal Production 11(2): 96-102 (2009) Key Words : Brahman Cross Cattle, embryo, PGF2α PMSG, FSH
The Effects of Super Ovulation on the Number of Corpus Luteum of Coastal Sumatera Cows (Bos sumatranensis) Depison, Depison; Adriani, Adriani; Rosadi, B
ANIMAL PRODUCTION Vol 13, No 1 (2011): January
Publisher : Universitas Jenderal Soedirman, Faculty of Animal Science, Purwokerto-Indonesia

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Abstract

The objective of this study was to observe the effects of super ovulation treatments on the number of ovulated corpus luteum. Metode   yang digunakan dalam penelitian adalah metode eksperimen. The method used in this study was experimental. Sapi-sapi diprogram untuk sinkronisasi estrus menggunakan PGF2ά. The cows were programmed to experience estrous at relatively the same time (estrous synchronization) using PGF2ά. Sapi-sapi tersebut kemudian dibagi secara acak menjadi empat perlakuan, masing-masing terdiri dari empat ekor sebagai ulangan. Data yang dihimpun dalam penelitian ini adalah jumlah corpus luteum, dan jumlah folikel tetap. Cows were divided randomly into four treatments, each of which consisting of four heads. The number of corpus luteum and the settled follicles was collected during the study. Data dianalisis menggunakan Rancangan Bujur Sangkar Latin, jika perlakuan berpengaruh nyata maka dilajutkan dengan uji lanjut Duncan. Data were analyzed using a Latin Square design. In case there was a significant difference, Duncan test was used thereafter. Result showed that Hasil penelitian menunjukkan bahwa penggunaan PGF2ά dalam pelaksanaan sinkronisasi estrus berjalan cukup baik.Hasil penelitian ini menunjukkan bahwa perlakuan superovulasi berpangaruh nyata terhadap Corpus Luteum dan folikel menetap.super ovulation had a significant effect on corpus luteum and settled follicles. Kesimpulan penelitian ini  protokol terbaik superovulasi tahun pertama adalah T-3 karena menghasilkan Corpus luteum dan Embrio yang lebih banyak.In conclusion, the best super ovulation protocol in the first year was the combination of 4 day daily injection of 40 mg of FSH in decreasing dose plus 250 µg of GnRH given at the time of estrous, because it was able to yield greater number of corpus luteum and embryos. (Animal Production 13 (1):52-56 (2011)Key Words: corpus luteum, follicle, super ovulation, Coastal Sumatera Cow
Co-Authors ., GUSHARIYANTO A. S. Wilastra Abdul Azis Abdul Azis Ade Octavia Adelia, Siska ADRIANI ADRIANI Adriani Adriani Adzitey, Frederick Afriani Afriani Agus Susilo Ahmad Taofik Almakmum, Husni Amalia Fildzah Ghassani Anak Agung Istri Sri Wiadnyani Anie Insulistyowati, Anie Annisa Rianti Ardi Novra Ardiantoro, Ari Arfiana, BS Monica Asa Bela Sri Reformasi Nala Putri Asa Bela Sri Reformasi Nala Putri Asep Gunawan Avrilliani, Puspita B., SUPRIAWAN Bawah , Juliana Bayu Rosadi Bella Pratiwi Putri Berliana Berliana Berliana Berliana Cece Sumantri Dea Tri Ananda Dika Irmaya E. Wiyanto Eko Wiyanto Eko Wiyanto Eko Wiyanto, Eko Endri Musnandar Erina, S. Ester Restiana Endang Gelis Fauzan Ramadan Fetty Febriana Rahayu Fitra Diansah Gushairiyanto Gushairiyanto Gushariyanto Gushariyanto Gushariyanto Gushariyanto Gushariyanto, Gushariyanto H. Ediyanto H. Suryani Haris Lukman Hariski, M Helmi Ediyanto Heru Handoko Heru Handoko Hidayat, Gradiandri Hosen, Tanjir Hossain, Md. Murad Husni Almakmum Irmaya. H, Dika Islam, Nazmul Jalius Jalius L. H. M. Gultom Lisna Lisna Lisna Lisna, Lisna Lisna, Lisna M., SAMIUN Mithun, Mahmudul Hasan Monten, Stephen K. Kanten Muhammad Abdu Muhammad, Mus'ab Muqsita Rahmat Nabilah Ika Puteri Nasution, Annio Indah Lestari Nelwida Nelwida Nurhayati Nurhayati Nurul Huda Pakpahan, Roberyo Pratama, Bondan Pratama, Shera Aditia Prayoga Alfano Putri, Asa Bela Sri Reformasi Nala R. A. Muthalib Ratna Sholatia Harahap Redo Prawira Rhaphon Seprian. Ht Riris Roiska Rizky Janatul Magwa S. Erina Salsabila Salsabila Septy Heltria Setiyawan, Khoirudin Anton Shalsabila Novia Al-Sobri Sidik, Wahid Hasyim Silvia Erina Silvia Erina Silvia Erina Silvia Erina Silvia Erina Silvia Erina Sofi Crisdayanti Suyadi Suyadi Talukder, Md. Khyrul Alam Triana Mardiah Utama, Millisani W. P. B. Putra Wahidu Zzaman Wahyuni Wahyuni Widya Pintaka Bayu Putra Wiwaha Anas Sumadja Y. Alwi Yun Alwi Zafitra A.