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Journal : Buletin Peternakan

Growth Patterns, Body Weight, and Morphometric of KUB Chicken, Sentul Chicken and Arab Chicken Depison Depison; Nabilah Ika Puteri; Gushairiyanto Gushairiyanto
Buletin Peternakan Vol 44, No 3 (2020): BULETIN PETERNAKAN VOL. 44 (3) AUGUST 2020
Publisher : Faculty of Animal Science, Universitas Gadjah Mada

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.21059/buletinpeternak.v44i3.57016

Abstract

The purpose of this research was to determine the body weight, weight gain and morphometric characteristics of several local chicken strains. The research materials were KUB chicken, Sentul chicken, and Arab chicken. The method used was an experiment with a sample of 82 chickens from each strain. Data collected include body weight, weight gain, beak length, beak width, head length, head circumference, head height, neck length, neck circumference, wing length, back length, back height, chest length, chest width, shank length, shank circumference, tibia length, tibia circumference, third finger length, and pubic bone distance. Data collected were analyzed using the average difference test (t-test). The average value vector of body measurements of chicken lines was analyzed using the T²-Hotelling statistical test. Main Component Analysis statistical test was used to identify the shape and size characteristics of each chicken strain. The results showed that the body weight and morphometrics of KUB chickens at 3 months were significantly different (P<0.05) with Sentul chickens and Arab chickens. KUB chicken`s body weight gain at 3 months was not significantly different (P>0.05) from Sentul chickens, but it was significantly different (P<0.05) from Arab chickens. Body weight, weight gain, and morphometrics of KUB chickens were higher than Sentul chickens and Arab chickens. The size indicator in KUB chickens was the tibia length while in Sentul chickens and Arab chickens was chest length. The characteristic of KUB chickens was the back length, Sentul chickens was tibia length, and Arab chickens was the shank circumference.
Comparison of Productivity of Sentul and Kampung Chickens until the Age of 3 Months in the First Generation Selection Population (G1) Wahyuni Wahyuni; Depison Depison; Gushariyanto Gushariyanto
Buletin Peternakan Vol 46, No 1 (2022): BULETIN PETERNAKAN VOL. 46 (1) FEBRUARY 2022
Publisher : Faculty of Animal Science, Universitas Gadjah Mada

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.21059/buletinpeternak.v46i1.67045

Abstract

This study aimed to compare the productivity of Sentul chickens and first-generation Kampung chickens (G1) until the age of 3 months. The research material were Sentul chicken and Kampung chicken. The method used was an experiment method or direct observation with a sample of 174 chickens from each strain. The data collected were egg weight, doc weight, body weight, body weight gain, body measurements, and selection response. Data on egg weight, body weight, and body measurements were analyzed using the average difference test (t-test), while the average value vector of body measurements Sentul chicken and Kampung chicken were analyzed using the T2-Hotelling statistical test. To identify the body size and body shape characteristics of Sentul chickens and Kampung chickens were analyzed using principal component analysis. Data processing used the statistical software Minitab version 18. The results of this study showed that egg weight, body weight at the age of DOC-3 months and body measurements of Sentul chickens were significantly different (P<0.05) higher than the Kampung chickens. This study concludes that the egg weight, body weight, body weight gain, and body sizes of Sentul chickens are higher than Kampung chickens. The size characteristic of Sentul and Kampung chickens is chest circumference. The characteristic of the shape of the Sentul chicken is the length of the wings, while the shape of the native chicken is the width of the chest. The selection response and the heritability value of the Sentul chickens was higher than Kampung chikens.  
Phenotype Diversity and Gene Myostatin (MSTN) of Bangkok Chicken using PCR-RFLP Triana Mardiah; Depison Depison; Helmi Ediyanto
Buletin Peternakan Vol 45, No 4 (2021): BULETIN PETERNAKAN VOL. 45 (4) NOVEMBER 2021
Publisher : Faculty of Animal Science, Universitas Gadjah Mada

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.21059/buletinpeternak.v45i4.68968

Abstract

This study aims to characterize the phenotype and determine the diversity of the Myostatin (MSTN) gene at Bangkok chickens using the Polymerase Chain Reaction- Restriction Fragment Length Polymorphism (PCR-RFLP) method. This study used 50 Bangkok chicken blood samples taken from the axillary vein on the wing. DNA was extracted using the protocol Genomic DNA Purification Kit from Promega and then amplified by PCR (polymerase chain reaction) using a pair of primers F: 5'GGT TTT GAC GAC ATG AGC CT3' R: 5'CAG GTG GAA TGT CAT GCA GA3' with product length 955 bp. Amplification products were cut using restriction enzyme MboI with cutting site ↓GATC. MSTN|MboI fragments of the Bangkok chicken were electrophoresed using 2% agarose gel and visualized using doc gel. The average difference test (T-test) on body weight and weight gain of Bangkok chickens from DOC to 3 months by gender. Polymorphism analysis includes allele frequency and genotype. Male and female Bangkok chickens have low phenotype diversity. The MSTN|MboI gene fragment is monomorphic with band positions of 492 bp, 244 bp, and 219 bp resulting in a genotype of ++, and there is one type of allele with a + allele frequency of 100%. 
Association of Quantitative Characteristics with Growth Hormone Gene (GH Gene) in Kerinci Duck Using PCR-RFLP Method Amalia Fildzah Ghassani; Depison Depison; Helmi Ediyanto
Buletin Peternakan Vol 46, No 4 (2022): BULETIN PETERNAKAN VOL. 46 (4) NOVEMBER 2022
Publisher : Faculty of Animal Science, Universitas Gadjah Mada

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.21059/buletinpeternak.v46i4.75810

Abstract

This study aims to determine the association of quantitative characteristics with growth hormone gene (GH gene) in kerinci duck using PCR-RFLP method. Samples total used was 96 Kerinci ducks consisting of 43 males and 53 females and 96 blood samples. DNA was extracted using the protocol Genomic DNA Purification Kit from Promega and then amplified by PCR using a pair of primers5&#39;-CAA GGA ACA GAG GGT TTC CA-3&#39; and Revers : 3&#39;-GGG AGA TAG GGC AAA CAT CA-5&#39;, with a length of product 855 bp. The amplification product was cut using restriction enzyme AluI with the AG↓CT cutting site. Growth hormone/AluI fragments of Kerinci duck were electrophoresed using 1.5% agarose gel and visualized using doc gel. Data collected includes body weight, weight gain, body measurements, and blood of Kerinci ducks. The differences in body weight, body weight gain, and body measurements, as well as differences in body weight between genotypes were tested by T-test. The determinants of the size and shape of Kerinci duck were analyzed using PCA. This study showed that the body weight, weight gain, and body sizes of male Kerinci ducks were significantly different (P<0.05) than female Kerinci ducks. The Kerinci duck GH|AluI gene is polymorphic with three genotypes, i.e, +/+ of (49%), +/- of (39.6%), and -/- of (11.5%), and two alleles, namely (+) by 69% and (–) by 31%. Conclusion: body weight, weight gain, and body sizes of male Kerinci ducks were higher than female. The size identifier of male and female Kerinci ducks were sternum, shank lengh, and shank circumference, while shape identifier was wing length. GH Genes|AluI in Kerinci duck is polymorphic. GH gene |AluI of Kerinci ducks was associated with body weight, weight gain, and body measurements, and the best is the genotype (+/+).
Association of Growth Hormone Gen with KUB Chicken Productivity Prayoga Alfano; Depison Depison; Silvia Erina
Buletin Peternakan Vol 47, No 3 (2023): BULETIN PETERNAKAN VOL. 47 (3) AUGUST 2023
Publisher : Faculty of Animal Science, Universitas Gadjah Mada

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.21059/buletinpeternak.v47i3.83902

Abstract

This study aims to investigate the diversity of quantitative characteristics and GH genes, along with the association between GH genes and quantitative characteristics. The research material consisted of 96 KUB chickens aged DOC-2 months and corresponding blood samples. The t-test was utilized to determine differences in body weight, weight gain, and body measurements between male and female KUB chickens, and assess the diversity of the GH gene. T2-Hotelling analysis was employed to compare body measurements between male and female KUB chickens, while principal component analysis was used to identify size and shape characteristics. Male KUB chickens' average body weight, weight gain and body size were significantly (p<0.05) higher than females. The analysis of the growth hormone gene exhibited three genotypes: +/+ (0.51), +/- (0.35), and -/- (0.14). Additionally, two alleles were identified: (+) accounting for 0.68% and (-) accounting for 0.32%. The growth hormone gene MspI of KUB chickens demonstrated polymorphism, with X2 count (2.93) < X2 table 0.05 (3.84). The heterozygosity value in the KUB chicken population was 0.43, and the obtained PIC value was 0.38. The genotype +/+ of the growth hormone gene in KUB chickens was significantly higher (p<0.05) than genotypes +/- and -/-.In conclusion, male KUB chickens exhibited higher body weight, weight gain, and body measurements compared to female KUB chickens. The chest circumference served as the identifier for body size in both male and female KUB chickens, while the length of the upper body and tibia length distinguished the shape characteristics of male and female KUB chickens, respectively. KUB chickens' growth hormone gene MspI was associated with body weight, weight gain, and body measurements, with the (+/+) genotype being the most favorable
The Quantitative Characteristics of Cross-Bred Native Chicken (Sentul X Arab Chicken and Merawang X Arab Chicken) Irmaya. H, Dika; Suyadi, Suyadi; Depison, Depison; Ardiantoro, Ari
Buletin Peternakan Vol 48, No 3 (2024): BULETIN PETERNAKAN VOL. 48 (3) AUGUST 2024
Publisher : Faculty of Animal Science, Universitas Gadjah Mada

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.21059/buletinpeternak.v48i3.89400

Abstract

The research aims to analyze the production potential and opportunities to increase the productivity of each cross-bred animal based on its quantitative characteristics. The livestock material used in this research was 50 Sera chickens (Sentul chickens x Arabian chickens) and 50 Mera chickens (Merawang chickens x Arabian chickens). The research was carried out by raising chickens from DOC to 3 months of age. The quantitative data involve body weight, weight gain, and body measurements (Head Length, Head Circumference, Head Height, Neck Length, Neck Circumference, Wing Length, Back Length, Back Height, Chest Length, Chest Width, Shank Length, Shank Circumference, Length Third Finger and Pubic Bone Distance). The quantitative data that has been observed is then analyzed through the t-test. Then, PCA is used to identify body shape and size determinants in Sera and Mera chickens. Statistical analysis was carried out using Minitab software version 21. The results obtained showed that the body weights of Sera and Mera chickens were significantly different (p<0.05) from DOC to 3 months of age. A significant increase in productivity in crosses between Sentul chickens and Arabian chickens and Merawang chickens with Arabian chickens occurs starting at 1-2 months. Sera and Mera chickens' body sizes differed significantly (p<0.05). The total diversity of PC1 in Sera chickens was 79.8%, and in Mera chickens, it was 70.6%. Meanwhile, the total PC2 diversity of Sera chickens was 17.7%, and Mera chickens were 7.9%. In conclusion, Sera chickens have the highest quantitative characteristics compared to Mera chickens, and the highest body weight gain occurs at 1-2 months in both Sera and Mera chickens.  Based on principal component analysis. The body size characteristic of Sera chickens is shank length, while in Mera chickens, it is chest length. The distinct shape of Sera chickens is body height, while in Mera chickens, it is shank length.
Co-Authors ., GUSHARIYANTO A. S. Wilastra Abdul Azis Abdul Azis Ade Octavia Adelia, Siska ADRIANI ADRIANI Adriani Adriani Adzitey, Frederick Afriani Afriani Agus Susilo Ahmad Taofik Almakmum, Husni Amalia Fildzah Ghassani Anak Agung Istri Sri Wiadnyani Anie Insulistyowati, Anie Annisa Rianti Ardi Novra Ardiantoro, Ari Arfiana, BS Monica Asa Bela Sri Reformasi Nala Putri Asa Bela Sri Reformasi Nala Putri Asep Gunawan Avrilliani, Puspita B., SUPRIAWAN Bawah , Juliana Bayu Rosadi Bella Pratiwi Putri Berliana Berliana Berliana Berliana Cece Sumantri Dea Tri Ananda Destiani, Dinda Dika Irmaya E. Wiyanto Eko Wiyanto Eko Wiyanto Eko Wiyanto, Eko Endri Musnandar Erina, S. Ester Restiana Endang Gelis Fauzan Ramadan Fetty Febriana Rahayu Fitra Diansah Gushairiyanto Gushairiyanto Gushariyanto Gushariyanto Gushariyanto Gushariyanto Gushariyanto, Gushariyanto H. Ediyanto H. Suryani Haris Lukman Hariski, M Helmi Ediyanto Heru Handoko Heru Handoko Hidayat, Gradiandri Hosen, Tanjir Hossain, Md. Murad Husni Almakmum Irmaya. H, Dika Islam, Nazmul Jalius Jalius L. H. M. Gultom Leni, Yusyam Lisna Lisna Lisna Lisna Lisna, Lisna Lisna, Lisna M., SAMIUN Mithun, Mahmudul Hasan Monten, Stephen K. Kanten Muhammad Abdu Muhammad, Mus'ab Muqsita Rahmat Nabilah Ika Puteri Nasution, Annio Indah Lestari Nelwida Nelwida Nurhayati Nurhayati Nurul Huda Pakpahan, Roberyo Pratama, Bondan Pratama, Shera Aditia Prayoga Alfano Putri, Asa Bela Sri Reformasi Nala R. A. Muthalib Ratna Sholatia Harahap Redo Prawira Rhaphon Seprian. Ht Riris Roiska Rizky Janatul Magwa S. Erina Salsabila Salsabila Septy Heltria Setiyawan, Khoirudin Anton Shalsabila Novia Al-Sobri Sidik, Wahid Hasyim Silvia Erina Silvia Erina Silvia Erina Silvia Erina Silvia Erina Silvia Erina Sinaga, Josua Sofi Crisdayanti Suyadi Suyadi Talukder, Md. Khyrul Alam Triana Mardiah Utama, Millisani W. P. B. Putra Wahidu Zzaman Wahyuni Wahyuni Widya Pintaka Bayu Putra Wiwaha Anas Sumadja Y. Alwi Yun Alwi Zafitra A.