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Journal : Makara Journal of Science

The Abundance of Nitrogen Fixing, Nitrifying, Denitrifying and Ammonifying Bacteria in the Soil of Tropical Rainforests and Oil Palm Plantations in Jambi Zulfarina,; Rusmana, Iman; Mubarik, Nisa Rachmania; Santosa, Dwi Andreas
Makara Journal of Science Vol. 21, No. 4
Publisher : UI Scholars Hub

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Abstract

Alterations in the use of land for oil palm plantations can change the domination and activity of soil bacteria. More specifically, alteration in soil microbial communities can directly affect soil ecosystem functioning, particularly with respect to carbon and nitrogen cycles. Nitrogen can be a limiting nutrient, and the availability of nitrogen in the soil environment becomes a major factor in controlling the production of biomass. This research project aimed at studying the abundance of nitrogen-fixing, nitrogen-oxidizing, nitrogen-reducing, and ammonifying bacteria based on their functional genes in the tropical rain forests of Taman Nasional Bukit Duabelas (TNBD) and the oil palm plantations in Sarolangun Jambi. Samples were collected in November 2015. Soil sampling was performed randomly at three points representing each area of ​​the tropical rainforests of TNBD and the seven- to eight-year-old oil palm plantations. Soil samples were collected using a soil sample core from 0–15 cm below the surface with depth strata of 0–5 cm, 5–10 cm, and 10–15 cm. Composite assessment was conducted on samples from each point corresponding to each respective depth strata. Soil samples were stored at -20ºC prior to testing. Microbial abundance was measured using the most probable number (MPN) method. The abundance of microbes that play a role in nitrogen metabolism between strata of 5–10 cm and 10–15 cm does not appear to be different. The highest abundance of microbes in oil palm plantation land in Jambi was found in samples with nitrifying bacteria, later followed by denitrifying, nitrogen-fixing, and ammonifying bacteria. Ultimately, it was found that microbial abundance in oil palm plantations was higher than the corresponding rates in samples from tropical rainforests.
Enrichment Media Selection and Co-Culture Potential among Exoelectrogen Bacteria Vary with Ecological Factors Khoirunnisa, Nur Syafira; Anwar, Syaiful; Sudadi, Untung; Santosa, Dwi Andreas
Makara Journal of Science Vol. 27, No. 1
Publisher : UI Scholars Hub

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Abstract

Staphylococcus saprophyticus ICBB 9554 and Citrobacter freundii ICBB 9763 are exoelectrogen bacteria applied as microbial fuel cells (MFC). We selected enrichment media for both these exoelectrogen bacteria, characterized their synergistic traits, and evaluated the growth conditions under different ecological factors. In this study, different enrichment media, such as those containing sugar, molasses, and palm sugar (2, 4, and 6% w/v) were tested for S. saprophyticus ICBB 9554. Meanwhile, technical sodium acetate (NaAc), commercial vinegar 25%, and cider vinegar (5, 10, and 15 mM acetate concentration) were tested for C. freundii ICBB 9763. Both the exoelectrogen bacteria were tested for the synergistic trait by inhibitory test, metabolic activity, and electricity generation performance in MFC. Different ecological factors, including salinity (2, 3, and 4 dS/m), culture media pH (5.8, 6.5, and 6.9), and temperature (20 °C, 27 °C, and 31 °C) were investigated for bacterial growth. The selective enrichment medium ingredient for S. saprophyticus ICBB 9554 was 2% molasses while that for C. freundii ICBB 9763 was 0.05 M technical sodium acetate. Both bacteria were suspected to grow synergistically and could be inoculated simultaneously as a co-culture in MFC. The effect of different ecological factors indicated that co-culture could grow better than pure culture. This study's findings provide important data on potential enrichment media for exoelectrogen bacteria that are beneficial for scale-up to reduce the operational cost.
Co-Authors . Giyanto . Giyanto Abdul Hasyim Sodiq Abdul Munif Agus Purwito Aisamrotul Hasanah Aksarah Pas, Aris Amanda Kusuma Dewi Anas Miftah Fauzi Arum Asiyanti Suhastyo, Arum Asiyanti Arum Asriyanti Suhastyo Atang Sutandi Batara, Lily Noviani Bedah Rupaedah Bibiana W Lay Bibiana W Lay BIBIANA W LAY Chansyanah Diawati Dedeh H Arief Dedi Widayat Dian Hendrayanti Didiek Hadjar Goenadi Didy Sopandie Dwi Wulan Pujiriyani Endriatmo Soetarto F. Dian Lina Fahrizal Hazra Falih, Fairuz Annabil Fitriyani, Indri Hapsari Giyanto, Giyanto Gunawan Djajakirana Gustav Adolf Wattimena Hamim Hamim HANNA ARTUTI EKAMAWANTI Hari R Harlyadi Harpasis S. Sanusi Harpasis S. Sanusi Haryanti Haryanti Hazrah, Fahrizal Hudi, Firdaus Alam I Dewa Ketut Sastrawidana Ilyas Jamil Iman Rusmana Indriyani, Yohanna Anisa Iskandar Iskandar Iswandi Anas Iswandi Anas Iswandi Anas Chaniago Iswandi Anas Chaniago, Iswandi Anas Ivanovich Agusta Juhari Juhari, Juhari Khoirunnisa, Nur Syafira Kukuh Murtilaksono Kurnia Dewi Sasmita Kusuma Handayani Laksmita Prima Santi Laksono Trisnantoro LILIK BUDIPRASETYO Maggy T Suhartono Memen Surahman Mieke Rochimi Setiawati Miftahudin Muchamad Yusron Muhammad Rivai Nasrul Harahap NISA RACHMANIA MUBARIK Noegroho Hadi Noor Fadiawati Nuchsin, Ruyitno NUNI GOFAR Nurfadila, Uci Nursaadah . RAHAYU WIDYASTUTI Rani Nur Fitriani Remaja Sitepu Rustam, Rustam Ruyitno Ruyitno S Siswanto Sanjaya, Wilhelmus Terang Arga Sari, Retno Purnama Siswanto siswanto Slamet Susanto Sri Wilarso Budi Sudarsono Sukmadewi, Desak Ketut Tristiana SULASTRI Suryo Wiyono Susiyanti . Syaiful Anwar T W DARMONO Titik Tri Wahyuni Tommy Kurniawan Tornando, Hengki Tri Prartono Trikoesoemaningtyas Tualar Slmarmata Umi Hidayati Umi Hidayati Untung Sudadi Wahono Sumaryono Widrializa, . Wilhelmus Terang Arga Sanjaya Wilhelmus Terang Arga Sanjaya YADI SETIADI Yeti Darmayati Yeti Darmayati Yohanna Anisa Indriyani YULIN LESTARI Yuni Lisafitri, Yuni Yuyun Sumarni Zulfarina Zulfarina Zulfarina,