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All Journal Jurnal Veteriner Jurnal Ilmu-Ilmu Peternakan (Indonesian Journal of Animal Science) Jurnal Layanan Masyarakat (Journal of Public Service) Journal of Parasite Science Ovozoa: Journal of Animal Reproduction Indian Journal of Forensic Medicine & Toxicology Jurnal Agro Veteriner
Emy Koestanti Sabdoningrum
Department Of Animal Husbandry - Faculty Of Veterinary Medicine, Universitas Airlangga
Agriculture, Biological Sciences & Forestry Humanities Decision Sciences, Operations Research & Management Environmental Science Immunology & microbiology Public Health Social Sciences Veterinary Other

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Journal : Ovozoa: Journal of Animal Reproduction

 1 
Tomato (Lycopersicon esculentum Mill.) juice restored the number of Leydig cells, and the diameter of the seminiferous tubules of mice (Mus musculus) exposed to lead acetate Revina Ayu Septiani; Iwan Sahrial Hamid; Emy Koestanti Sabdoningrum; Anwar Ma'ruf; Eka Pramyrtha Hestianah; Maslichah Mafruchati
Ovozoa: Journal of Animal Reproduction Vol. 11 No. 3 (2022): Ovozoa: Journal of Animal Reproduction
Publisher : Universitas Airlangga

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20473/ovz.v11i3.2022.123-129

Abstract

Lead is a harmful pollutant from engine exhaust that causes free radicals and has detrimental effects on the testicular tissue. This study aimed to determine the effects of tomato juice on the number of Leydig cells and the diameter of the seminiferous tubules of mice exposed to lead acetate. Twenty-five male mice were divided into five groups. Mice in the control (C-) group were given placebos. Meanwhile, mice in C+, T1, T2, and T3 groups were exposed to lead acetate at a dose of 100 mg/kg BW/day for 14 days and given tomato juice respectively at 0, 0.16, 0.32, and 0.64 mL/day from day 8 to day 35. On day 36, all mice were sacrificed, and the testes were collected for histological preparation. The result showed that lead exposure in the C+ group decreased (p <0.05) in the number of Leydig cells and the diameter of the seminiferous tubules compared to the mice in group C-. Administration of tomato juice in groups T1, T2, and T3 increased (p <0.05) the number of Leydig cells and the diameter of seminiferous tubule compared to the mice in the C+ and C- groups. However, tomato juice administration to the T3 group decreased the number of Leydig cells and the diameter of the seminiferous tubules (p <0.05) compared to the T2 group. In conclusion, an effective dose of 0.32 mL/day of tomato juice restored Leydig cell number and seminiferous tubules diameter in mice exposed to lead acetate.
Potential of Basil (Ocimum basilicum) leaf extract in Tris-egg yolk extender on sperm motility and plasma membrane integrity of Sapudi ram at room temperature Pratama Putra Banusiandana; Suherni Susilowati; Sri Mulyati; Tri Wahyu Suprayogi; Emy Koestanti Sabdoningrum; Nove Hidajati
Ovozoa: Journal of Animal Reproduction Vol. 14 No. 3 (2025): Ovozoa: Journal of Animal Reproduction
Publisher : Universitas Airlangga

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20473/ovz.v14i3.2025.119-126

Abstract

This study aimed to determine the effect of basil (Ocimum basilicum) leaf extract in a Tris-egg yolk extender on the motility, and plasma membrane integrity of Sapudi ram spermatozoa at room temperature. Fresh semen samples were collected from Sapudi ram using an artificial vagina. A completely randomized design was used, consisting of four treatment groups with five replicates each. Data were analyzed using SPPS with factorial analysis of variance (ANOVA) and Duncan’s test. The control group (T0) received Tris-egg yolk extender without basil leaf extract. Treatment groups T1, T2, and T3 received 1%, 2%, and 3% basil leaf extract, respectively, in the extender. Results showed that sperm motility and plasma membrane integrity (Table 4) tended to decrease with increased storage time. Among the treatment groups, the addition of 1% basil leaf extract in the Tris-egg yolk extender (T1) resulted in significantly higher (p <0.05) both sperm motility and plasma membrane integrity compared to the control (T0). However, higher concentrations of basil leaf extract (T2 and T3) did not result in significant differences (p >0.05) in sperm motility after three hours of storage compared to the 1% treatment (T1). In conclusion, the addition of 1% basil leaf extract to a Tris-egg yolk extender was effective in maintaining sperm motility and plasma membrane integrity of Sapudi ram spermatozoa stored at room temperature for up to three hours.
Co-Authors Agustono Agustono Al arif, Mohammad Anam Anam Al-Arif Anita Nur Aida Aida Anwar Ma'ruf Bayu Dwi Saputra Dian Ayu Permatasari Eka Pramyrtha Hestianah Elita Triwijayanti Aqfari Endang Suprihati Fadila Zikri Amanda Febriano, Muhammad Galang Hana Eliyani Hana Eliyani Heru Pramono Iwan Sahrial Hamid Kadek Rachmawati Koesnoto Supranianondo Lina Wafia Asmi Mafruchati, Maslichah Melanie Aulia Ashfiyah Mirni Lamid Moh. Zaky ‘Ubaidillah Muchammad Yunus Mufasirin Myrna Budi Nove Hidajati Nunuk Dyah Retno Lastuti Poedji Hastutiek Pratama Putra Banusiandana Pudji Srianto Rahmaniar Larasati Rara Irbah Fitriana Karimah Revina Ayu Septiani Setiawan Koesdarto Siti Eliana Soeharsono Soeharsono Soeharsono Soeharsono Sri Hidanah Sri Hidanah Sri Mulyati Suherni Susilowati Suwarno Tatik Hernawati Tri Wahyu Suprayogi Virgy Dzakina Fahrezy Warsito, Sunaryo Hadi Widya Paramita Lokapirnasari Widya Paramita Lokapirnasari Wiwik Misaco Yuniarti Woro Hastuti Setyantini