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All Journal JBIO: jurnal biosains (the journal of biosciences) Indonesian Journal of Tropical and Infectious Disease Biota Bioeksperimen: Jurnal Penelitian Biologi Journal of Tropical Biodiversity and Biotechnology Indonesia Journal of Halal Indonesian Journal of Halal Research Journal of halal product and research (JHPR) Jurnal Riset Biologi dan Aplikasinya Sains dan Matematika Genbinesia Journal of Biology BIOTROPIC
Yuanita Rachmawati
UIN Sunan Ampel Surabaya
Religion Agriculture, Biological Sciences & Forestry Humanities Astronomy Biochemistry, Genetics & Molecular Biology Chemical Engineering, Chemistry & Bioengineering Chemistry Dentistry Earth & Planetary Sciences Economics, Econometrics & Finance Education Environmental Science Health Professions Immunology & microbiology Materials Science & Nanotechnology Mathematics Medicine & Pharmacology Physics Public Health Social Sciences Veterinary Other

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CmBGI Gene Expression encoding β-glucosidase in melon (Cucumis melo L.) under stress condition Yuanita Rachmawati; Ganies Rizaa Aristya; Budi Setiadi Daryono
Biotropic : The Journal of Tropical Biology Vol. 1 No. 2 (2017): Biotropic, Volume 1, Nomor 2, 2017
Publisher : Program Studi Biologi, Fakultas Sains dan Teknologi, Universitas Islam Negeri Sunan Ampel Surabaya

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.29080/biotropic.2017.1.2.1-8

Abstract

CmBGI is the enzymatic genes encoding β-glucosidase that involved in Abscisic Acid (ABA) metabolism of Cucumis melo L. β-glucosidase promotes the accumulation of glucose, fructose, and sucrose, and it might act as a regulator that mediates melon fruit ripening both climacteric and nonclimacteric. ABA mediates adaptive responses to abiotic and biotic stresses. Agricultural Balitbang in 1997 showed that there were approximately 158.600 ha of degraded land scattered in three zones of agroecosystems in Yogyakarta (DIY). One of them is Dlingo Bantul area which has a karst type critical land area. Karst provides stress to the certain plant growth. One way to conserve critical land is making this area for agriculture. Cultivar TACAPA and TA were superior melons that have been developed by Genetic Laboratory of Biology Faculty UGM. This preliminary research was conducted to examine molecular characterization of CmBGI gene expression in cultivar TACAPA and TA which are planted in normal condition medium and in critical land medium treatment. Total RNA was extracted from leaf tissue then Reversed Transcriptase (RT-PCR) to collect cDNA library. cDNA was amplified using specific primer. Spectrophotometry was conducted in λ260 nm and electrophoresis run in 1.5% agarose gel. Control of band chosen was Cm-Actin. CmBGI gene concentration of TACAPA and TA in normal condition medium are in succession 578.5 and 579.4 μg/ml then for critical land medium treatment 743.4 and 773.5 μg/ml. CmBGI band was showed both of TACAPA and TA as ± 1258 bp. Cm-actin was showed band of DNA as ± 445 bp. CmBGI gene concentration in critical land medium treatment which is given greater stress on melons are higher than normal condition. This suggests that the CmBGI gene is expressed more in cultivar TACAPA and TA melons when they are grown under stress condition.
Phenotipical Characters of Melon (Cucumis melo L.) in Response to Karst Critical Land Yuanita Rachmawati; Budi Setiadi Daryono; Ganies Riza Ariestya
Biotropic : The Journal of Tropical Biology Vol. 2 No. 1 (2018): Biotropic, Volume 2, Nomor 1, 2018
Publisher : Program Studi Biologi, Fakultas Sains dan Teknologi, Universitas Islam Negeri Sunan Ampel Surabaya

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.29080/biotropic.2018.2.1.1-10

Abstract

Yogyakarta Agroecosystem has 158,600 ha of critical land spread over three zones. Two areas are Karst Land, located on Agroecosystem II includes Gunungsewu Hills, Gunungkidul and III covers Dlingo Bantul Hills and Sentolo Hills Kulon Progo Regency.. Karst Land is certainly provides stress to plants. These research purposes are examining the phenotype character of superior melon Cultivar TACAPA compare to parents and offsprings phenotypes. The phenotype characters are based on plant height, leaf number, time of melon flowering, water content of plants, and fruit and seed productivity. This experiment was done by Split Plot Design with Completely Randomized Design (CRD) with 4 kinds of treatment (control plant media, Gunungsewu, Dlingo, and Sentolo), 7 experimental units cultivars: TACAPA, TA, TP, PT, AT, Action 434, PI 371795), and 4 replications. Research result reveals that most of the phenotypic characters including plant height, number of leaves, fruit weight, and number of seeds produced have relatively no significant effect between treatment and control, while the phenotypic first time flowering time and water content of the plant, have a noticeable difference.
Comparison of DNA Isolation Results with Simple Methods and Kits in Samples of Psidium guajava Leaves Yuanita Rachmawati; Romyun Alvy Khoiriyah
Biotropic : The Journal of Tropical Biology Vol. 2 No. 2 (2018): Biotropic, Volume 2, Nomor 2, 2018
Publisher : Program Studi Biologi, Fakultas Sains dan Teknologi, Universitas Islam Negeri Sunan Ampel Surabaya

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.29080/biotropic.2018.2.2.93-99

Abstract

DNA isolation is one of a series of methods that must be carried out on the basic techniques of Molecular Biology Analysis. Especially PCR-based molecular marking techniques. Many ways are done in DNA isolation. This study discusses the comparison of the results of DNA isolation using two methods. Simple DNA isolation methods and using Kit. Samples of Psidium guajava leaves used were taken from 15 different locations used. In general, DNA isolation methods include three steps, namely destruction, precipitation, and purification. Simple DNA isolation is done with detergents, alcohol groups, which are commonly available in the laboratory. Methods of DNA isolation with KIT are carried out according to the Promega Universal Wizard KIT protocol. The comparison results are seen from spectrophotometric absorption Å230 nm, Å260 nm, Å280 nm, Å320 nm, ratio Å260/Å230, ratio Å260/Å280 to see DNA purity, protein concentration before purification step, and DNA concentration produced. The results showed that there were no statistically significant differences in the results of DNA isolate spectrophotometry. However, the use of KIT with modified protocols is more recommended if researchers want to carry out DNA analysis more precisely and accurately. Keywords: DNA isolation, spectrophotometry, DNA concentration and purity
Chromosome Characterization of Brassicaceae Family Ganies Riza Aristya; Bening Larasati; Galang Riswi Dyatama; Himawan Masyhuri; Febri Yuda Kurniawan; Fauzana Putri; Dian Astriana; Yuanita Rachmawati
Biotropic : The Journal of Tropical Biology Vol. 6 No. 2 (2022): Biotropic, Volume 6 Nomor 2, 2022
Publisher : Program Studi Biologi, Fakultas Sains dan Teknologi, Universitas Islam Negeri Sunan Ampel Surabaya

Show Abstract | Download Original | Original Source | Check in Google Scholar

Abstract

Indonesia is known as a rich country in various agricultural and plantation products, including vegetables such as mustard, broccoli, cabbage, and cauliflower. However, in its cultivation, the products and demand for vegetables are not offset by an increase in the production quality. One of the efforts to improve and enhance the production quality is to identify and characterize chromosomes of plants which will become the basis for plant breeding activities. The purpose of this study was to characterize the number, form and size of the chromosome in cultivars belonging to the Brassicaceae family. The study was carried out using the modified squash method. Chromosomes were prepared by fixation, maceration, and staining, then the mitotic phases were observed using a microscope and optilab, and analyzed using Image Raster 3. The results showed that mitotic time range and chromosome character of six cultivars of the Brassicaceae family were different. Broccoli ('Chief No. 2 1955' and 'Green Super') and cauliflower ('ILONA' and 'TM 126') had a mitotic time range from 04.00 to 09.00 a.m. with 2n chromosome number = 18. Green mustard (‘Juwita’ and ‘TM Jade’) and white mustard ('Sakata' and 'Shuka-shuka') had a mitotic time range from 03.00 to 08.00 a.m. with 2n chromosome number = 20. White cabbage (‘CR ACE' and 'Sehati F1') had a mitotic time range from 04.00 to 09.00 a.m and red cabbage (‘Scarlet’ and ‘Red Globe’) had a mitotic time range from 09.00 to 10.00 a.m. with 2n chromosome number = 18.    
Optimization of RNA Extraction from Aedes aegypti and Aedes albopictus Saiku Rokhim; Ninik Fadhillah; Radinal Kautsar; Humayra Qurrata Aini; Yuanita Rachmawati
Biotropic : The Journal of Tropical Biology Vol. 7 No. 1 (2023): Biotropic, Volume 7 Nomor 1, 2023
Publisher : Program Studi Biologi, Fakultas Sains dan Teknologi, Universitas Islam Negeri Sunan Ampel Surabaya

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.29080/biotropic.v7i1.1781

Abstract

RNA extraction is the critical initial stage in analyzing certain gene expressions, further analysis using Real Time PCR technology, and performing virus detection. However, the process of extracting RNA is often hampered by the risk of contamination, resulting in low concentrations of RNA and low purity of RNA. This is often an obstacle in extracting mosquito RNA especially detecting Dengue Virus (Den-V). Dengue virus (Den-V) can cause dangerous diseases in humans such as Dengue Fever (DHF) which is transmitted through the bites of Aedes aegypti and Aedes albopictus mosquitoes. This study aims to find out the effective steps for extracting RNA from Aedes aegypti and Aedes albopictus mosquitoes. The method being compared is a commercial RNA extraction kit with modification (addition of β-mercaptoethanol) and without modification. The results showed that the best DNA concentration and purity were obtained in mosquito samples from modified process. The purity ratio of RNA extracted without modification was 1.971 (0.021 ± 0.800) while with modification it was 2.003 (0.011 ± 0.112). Aedes aegypti had a better average concentration of 7.146 µg/ml for unmodified RNA and 7.613 µg/ml for modified RNA. This research is expected to be a reference for further studies on viruses in Aedes aegypti and Aedes albopictus.
Co-Authors Adzakiyyi, Miftahul Lathif AGUSTINA, EVA Aini, Humayra Qurrata Aini, Nur Sofiatul Ameliora C E Arrohmah, Robiatush Sholichah Ayudya Fitri Arifa Azzhahara, Nabillah Ayu Bening Larasati BUDI SETIADI DARYONO Choiriyah, S.Si, Chumaidatul DAMAYANTI, MAMIK Dian Astriana Eko Prasetya Eko Prasetya, Eko Esti Tyastirin Fauzana Putri Fauziyah Harahap Febri Yuda Kurniawan Firmansyah, M. Aliffiyan Galang Riswi Dyatama Ganies Riza Ariestya Ganies Riza Aristya Hary Prakasa Himawan Masyhuri Humayra Qurrata Aini Inggrit Tyautari Inggrit Tyautari Irul Hidayati Kautsar, Radinal KHOIRIYAH, ROMYUN ALVY Latifatoel Chilmi Lazuardi Lazuardi M. Aliffiyan Firmansyah Mabruroh, Arofatul Syabina Mahbubillah, M. Ainul Mashita Andiana, Mashita Miftahul Jannah Miftahul Lathif Adzakiyyi Misbakhul Munir Misbakhul Munir Moch. Irfan Hadi Nabila Ayu Nabila, Salsa Najwa Maulidina P Ninik Fadhillah Nirmala Fitria Firdhausi Oki Rahmatirta W Pradiptaadi, Brian Pramana Aprilio Puji Prastowo, Puji Putri, Siti Aisyah Bisri Radinal Kautsar Ramadhani, Aisyah Hadi Said . Iskandar Saiful Bahri Saiku Rokhim Setiyowati, Putri Ayu Ika Siti Latifa Siti Malihatus Sa'adah Sri Susila Andayani, Sri Susila Teguh Hari Sucipto, Teguh Hari Tri Susilowati Tri Susilowati Tri Susilowati Tyautari, Inggrit Yusuf, Yusnaeni