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All Journal Jurnal Ilmu Dasar Jurnal Sains dan Teknologi Biota: Jurnal Ilmiah Ilmu-Ilmu Hayati Jurnal Kesehatan Andalas Jurnal Pendidikan Biologi Panrita Abdi - Jurnal Pengabdian pada Masyarakat Jurnal Ilmiah Universitas Batanghari Jambi Majalah Ilmiah Biologi BIOSFERA: A Scientific Journal Jurnal Pengabdian Magister Pendidikan IPA Diseminasi : Jurnal Pengabdian kepada Masyarakat Jurnal Pembelajaran Pemberdayaan Masyarakat (JP2M) Madaniya Bioscientiae, Jurnal ilmu ilmu Biologi Jurnal Pemantik Molekul: Jurnal Ilmiah Kimia BIOTROPIC
Sulistiyawati, Indah
Universitas Nahdlatul Ulama Purwokerto
Religion Agriculture, Biological Sciences & Forestry Humanities Biochemistry, Genetics & Molecular Biology Chemistry Civil Engineering, Building, Construction & Architecture Computer Science & IT Control & Systems Engineering Economics, Econometrics & Finance Education Environmental Science Health Professions Immunology & microbiology Languange, Linguistic, Communication & Media Law, Crime, Criminology & Criminal Justice Library & Information Science Materials Science & Nanotechnology Mathematics Social Sciences Veterinary Other

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Profil Ekspresi MikroRNA Kanker Payudara di Purwokerto Nurasih, Ari Dwi; Sulistiyawati, Indah; Falah, Muhammad
Jurnal Kesehatan Andalas Vol. 9 No. 4 (2020): Online December 2020
Publisher : Faculty of Medicine, Universitas Andalas

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.25077/jka.v9i4.1553

Abstract

Penemuan metode deteksi dini kanker payudara secara sensitif, spesifik dan minimal invasif merupakan kunci dalam keberhasilan pengobatan. Hal ini dapat dilakukan menggunakan biomarker yang bersirkulasi dalam plasma darah yaitu mikroRNA. MikroRNA adalah asam ribonukleat yang tidak mengkode protein tetapi memiliki peran dalam menghambat dan mendegradasi mRNA sehingga mempengaruhi perkembangan kanker. Tujuan: Mengetahui profil ekspresi mikroRNA pada pasien kanker payudara di Purwokerto dan mikroRNA yang mengalami disregulasi sebagai kandidat biomarker kanker payudara. Metode: Analisis profil ekspresi mikroRNA pada kanker payudara dilakukan dengan isolasi plasma dari pasien kanker payudara dan individu sehat, sintesis cDNA, dan kuantifikasi mikroRNA dengan quantitative real time PCR (qPCR). Kemudian dilakukan penghitungan dengan metode Livak untuk mengetahui mikroRNA yang mengalami peningkatan dan penurunan ekspresi yang signifikan. Hasil:  Biomarker kanker payudara di Purwokerto adalah MikroRNA yang mengalami upregulation adalah hsa-miR-543 (sebanyak 1612,28 kali), hsa-miR-495-3p (1797,95 kali), hsa-miR-382-5p (8780,32 kali), hsa-miR-155-5p (8552,18 kali) dan hsa-miR-154-5p (2339,41 kali). mikroRNA yang mengalami downregulation adalah hsa-miR-590-5p (sebanyak 4,70 kali), hsa-miR-33a-5p (sebanyak 74,29 kali), hsa-miR-100-5p (sebanyak 23,09 kali), hsa-miR-19b-3p (sebanyak 11,71 kali) dan hsa-miR-144-3p (sebanyak 25,30 kali) (p < 0,05). Simpulan: Biomarker yang dapat digunakan sebagai alat deteksi dan diagnostik dini kanker payudara di Purwokerto adalah miR-382-5p.Kata kunci: biomarker, kanker payudara, mikroRNA, qPCR
Inovasi teknologi modern pengolahan sirup buah mangrove solusi strategis mewujudkan kemandirian ekonomi masyarakat pesisir Sulistiyawati, Indah; Farisi, Herdian; Karseno
Jurnal Pembelajaran Pemberdayaan Masyarakat (JP2M) Vol. 6 No. 4 (2025)
Publisher : Universitas Islam Malang

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.33474/jp2m.v6i4.24173

Abstract

Program pemberdayaan kemitraan masyarakat ini dilaksanakan di Desa Kutawaru, Segara Anakan, Cilacap dengan tujuan meningkatkan kapasitas dan kemandirian ekonomi masyarakat pesisir melalui inovasi teknologi pengolahan buah mangrove menjadi produk pangan bernilai tambah. Metode yang digunakan adalah pendekatan partisipatif (Participatory Action Research), meliputi wawancara awal, focus group discussion (FGD), pelatihan teknis berbasis partisipasi, serta pendampingan manajemen usaha yang disertai monitoring dan evaluasi. Kebaruan program terletak pada penerapan teknologi modern untuk mengolah buah mangrove menjadi minuman sirup berkualitas, disertai strategi diferensiasi melalui branding digital dan pemasaran daring. Hasil kegiatan menunjukkan peningkatan keterampilan produksi dan pengetahuan masyarakat sebesar 21,33%, terbentuknya diversifikasi produk olahan berbasis mangrove, tersusunnya sistem manajemen usaha sederhana, serta peningkatan akses pasar melalui platform digital. Capaian ini tidak hanya meningkatkan kapasitas individu, tetapi juga memperkuat jejaring ekonomi lokal dengan potensi peningkatan pendapatan dan keberlanjutan usaha. Dengan demikian, tujuan program untuk memberdayakan masyarakat pesisir berbasis inovasi teknologi dan strategi pemasaran modern dinyatakan tercapai, sekaligus menawarkan model pengabdian yang aplikatif dan replikatif bagi kawasan pesisir lainnya.
Antimicrobial Activity of Basil (Ocimum basilicum L.) Against Gram-Negative Bacteria Involved in Pneumonia Infection Sulistiyawati, Indah; Falah, Muhammad; Anggraeni, Gita; Rovik, Anwar
JST (Jurnal Sains dan Teknologi) Vol. 12 No. 2 (2023): July
Publisher : Universitas Pendidikan Ganesha

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.23887/jstundiksha.v12i2.53577

Abstract

Infectious diseases are still a global problem, for example, acute respiratory infections (SARI). Resistance in pneumonia involving Gram-negative bacterial infection reduces the effectiveness of antibiotic use and increases the length of hospital stay. This study examines the potential of antimicrobial compounds from basil plants against isolates of Gram-negative bacteria involved in pneumonia infection. This research is an experimental study through several stages: basil extraction, initial screening of bioactive compounds, isolation and identification of bacteria from sputum, and testing for antimicrobial activity of basil extracts. Sputum samples were collected from 43 pneumonia patients (male and female) involving Klebsiella pneumoniae, Acinetobacter baumannii, Enterobacter aerogenes, Pseudomonas aeruginosa, Serratia marcescens, Proteus mirabilis, and Escherichia coli. The inhibitory activity against pathogenic bacteria was directly proportional to the basil extract concentration. Initial screening for phytochemical compounds showed that the extracts of basil leaves and stems contained active compounds of tannins and flavonoids. The 25% concentration of basil extract was the minimum inhibitory concentration (MIC) against the test bacteria. The 100% basil extract concentration produced the largest inhibition zone i.e., 10.93 mm. The overall findings of this study provide baseline information for the possible use of the Ocimum basilicum extract in the treatment of pneumonia involving Gram-negative bacteria.
Upaya Meningkatkan Pendapatan Kelompok Ternak Lembu Rizki Melalui Branding dan Modernisasi Pengolahan Susu Pada Program Pemberdayaan Kemitraan Masyarakat Zulaikhah, Siti Rahmawati; Sulistiyawati, Indah; Nugroho, Adityo
Jurnal Pengabdian Magister Pendidikan IPA Vol 6 No 4 (2023): Oktober-Desember 2023
Publisher : Universitas Mataram

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.29303/jpmpi.v6i4.5509

Abstract

The Lembu Rizki Livestock Group is in Singasari Village, Karanglewas District, Banyumas Regency. The problems in this group are a) Low selling price of milk in cooperatives, b) Farmers do not yet have milk processing technology, c) Limited human resources, resulting in difficulties in production and marketing. The solutions offered are 1) Sales in the form of processed milk, 2) Transfer of diversification of milk processing technology in the form of pasteurized milk of various flavors and yoghurt, 3) Empowerment of family members, and marketing using digital marketing. The general objective of this Community Partnership Empowerment activity is a) to provide solutions based on situation analysis according to the needs, challenges or problems that exist in the Lembu Rizki livestock group, b) to empower the Lembu Rizki livestock group to improve their standard of living both in economic, social and cultural terms, c) transfer technology, science and art for the development of livestock groups. The results obtained from this activity are increased target knowledge and skills regarding processing milk into pasteurized milk of various flavors and yoghurt, knowing the importance of packaging and labeling for product branding, and digital marketing. Keyword: Branding, Modernization, Milk Processing, Empowerment of Community Partnerships, Livestock Groups
PEMERIKSAAN BAKTERI COLIFORM PADA SAMPEL DEPOT AIR MINUM ISI ULANG (DAMIU) Azizah, Teguh; Sulistiyawati, Indah
Bioscientiae Vol 23, No 1 (2026): Bioscientiae Volume 23 No 1
Publisher : Universitas Lambung Mangkurat

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20527/b.v23i1.15249

Abstract

Water is one of the basic human needs, because humans are very dependent on air for both drinking and daily life. Air quality that includes is very important to ensure safe drinking water, but it is also important to consider the broader impacts of air management practices. Refill Drinking Water Depot (DAMIU). This study aims to ensure that drinking water is safe and suitable for consumption. This study was to examine coliform bacteria in drinking water samples from Refill Drinking Water Depot (DAMIU) using the membrane filter method, with 10 samples. This study uses the Membrane filter method, which is a technique used to test water samples to identify the presence of coliform bacteria and Escherichia coli. The results of testing 10 drinking water samples can be concluded that some of the quality of drinking water at the research location is still different and has not fully met the quality standards. The results are not in accordance with the maximum limit of coliform and E. coli in drinking water is 0 CFU / 100 mL in the drinking water quality standards set by the Regulation of the Minister of Health of the Republic of Indonesia (PERMENKES) Number 2 of 2023. The influencing factors are raw materials, length of time the water is stored in the reservoir, handling of the buyer's container, operator cleanliness, and environmental cleanliness around the DAMIU which is less noticed.
UJI CEMARAN BAKTERI COLIFORM DAN Escherichia coli PADA AIR BERSIH SEBAGAI INDIKATOR KUALITAS LINGKUNGAN Kafah, Silmi; Sulistiyawati, Indah
Bioscientiae Vol 23, No 1 (2026): Bioscientiae Volume 23 No 1
Publisher : Universitas Lambung Mangkurat

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20527/b.v23i1.18192

Abstract

Clean water plays a vital role in supporting human health and daily activities. This research aimed to analyze microbiological contamination specifically coliform and Escherichia coli bacteria in clean water as indicators of environmental quality. The study employed the membrane filter method to examine 20 water samples collected from various sources including wells, springs, and PDAM water. Microbiological tests followed the standard procedures set by the Ministry of Health Regulation No. 2 of 2023. In addition to microbiological testing, chemical parameters such as pH and iron content were also analyzed using a pH meter and UV-Vis spectrophotometer. The results indicated that not all samples met the required quality standards. Several samples contained coliform and E. coli levels exceeding the permissible limit of 0 CFU/100 mL, making them unsuitable for consumption. High bacterial levels were linked to poor sanitation practices around the water sources. The study also found that a few samples had elevated pH levels and iron concentrations, which may pose health risks. This research contributes valuable insights into water quality monitoring and underlines the importance of environmental hygiene in ensuring safe water sources.
SKRINING SIFILIS MENGGUNAKAN Treponema pallidum RAPID PADA PEKERJA MIGRAN INDONESIA DI KABUPATEN BANYUMAS Rabbani, Fadhil; Sulistiyawati, Indah
Bioscientiae Vol 23, No 1 (2026): Bioscientiae Volume 23 No 1
Publisher : Universitas Lambung Mangkurat

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20527/b.v23i1.18190

Abstract

Syphilis is a sexually transmitted infection (STI) caused by Treponema pallidum subspecies pallidum that continues to pose a significant public health challenge, particularly among groups such as Indonesian migrant workers (PMI). Early detection is essential in preventing transmission and ensuring appropriate treatment. This study aimed to determine the prevalence of syphilis among Indonesian migrant workers using the Treponema pallidum Rapid Test (TP-Rapid) at a health facility in Banyumas Regency. The diagnostic procedure was conducted in three stages such as pre-analytic, analytic, and post-analytic. A quantitative descriptive method with a cross-sectional approach was employed involving 160 Indonesian migrant workers undergoing pre-departure health screening. Blood samples were collected and tested using the TP-Rapid method, which offers rapid, sensitive, and specific detection of T. pallidum antibodies. Results indicated that 10 out of 160 samples (6,25%) was reactive for syphilis, while 150 samples (93,75%) were non-reactive. Despite the relatively low prevalence, the findings underscore the necessity of routine syphilis screening. The TP-Rapid test was found to be a practical diagnostic tool due to its simplicity, rapid turnaround time, and reliability in identifying syphilis seroreactivity. These results highlight the importance of integrating early syphilis detection into mandatory health screenings for migrant workers to mitigate potential transmission and safeguard both individual and public health.
DETEKSI HEPATITIS B Surface Antigen (HBsAg) DENGAN PERBANDINGAN WAKTU DAN AKURASI RAPID TEST DAN Enzyme-Linked Immunosorbent Assay (ELISA) Zain, Nasywa Luthfi; Sulistiyawati, Indah; Muryanti, Muryanti
Bioscientiae Vol 23, No 1 (2026): Bioscientiae Volume 23 No 1
Publisher : Universitas Lambung Mangkurat

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20527/b.v23i1.18191

Abstract

Hepatitis B is one of the serious infectious diseases that is still a global health problem. Early detection of HBsAg infection is very important to prevent further complications. This study aims to compare the detection time and accuracy between the rapid test and Enzyme-Linked Immunosorbent Assay (ELISA) in detecting HBsAg. The study used two methods, then analyzed based on age and gender variables. The rapid test showed advantages in detection speed, but had limitations in accuracy. Meanwhile, ELISA takes longer but provides more sensitive and specific results. The results of this study indicate that the selection of diagnostic methods must consider clinical needs so that the combination of both methods can increase the effectiveness of early detection and control of Hepatitis B infection in the community.
Uji Ekstrak Bawang Putih Tunggal (Allium sativum L.) Terhadap Bakteriuria Escherichia coli Pada Calon Pekerja Migran Indonesia Indah Sulistiyawati; Muhammad Falah; Ari Dwi Nurasih
Biotropic : The Journal of Tropical Biology Vol. 5 No. 1 (2021): Biotropic, Volume 5 Nomor 1, 2021
Publisher : Program Studi Biologi, Fakultas Sains dan Teknologi, Universitas Islam Negeri Sunan Ampel Surabaya

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.29080/biotropic.2021.5.1.50-57

Abstract

Garlic (Allium sativum L) is a tested type of phytopharmaca because it is proven to have beneficial benefits as antibacterial and protect the body from pathogens. The majority of Indonesian Migrant Workers, especially Prospective Indonesian Migrant Workers (CPMI), are indicated to be susceptible to Urinary Tract Infection (UTI), which is caused by the bacteriuria Escherichia coli. In this study, the crude extract of single garlic (A. sativum L) will be used as an antibacterial against E. coli bateriuria in the urine sample of CPMI, with variations in the concentration of single crude extract of garlic, namely; 0%, 25%, 50%, 75%, and 100%. This research was conducted in a period of 1 (one) year from January to December 2020, with urine research samples from CPMI in the Banyumas Regency area. Extraction was carried out by extracting a single garlic using 96% ethanol solvent in a ratio of 1: 1, then evaporated to produce a filtrate that would be tested with a varied concentrate composition. Isolation of bacteriuria in urine samples using EMBA selective media. From a total of 37 urine samples, 17 about 46% were positive for E. coli, and 20 samples were 54% negative for E. coli. Bacteriuria that was found challenged with crude garlic extract resulted in differences in the antibacterial test at several extract concentrations with amoxicillin positive control, with One Way Anova a P-value = 0.000 <0.05, and continued with the analysis of the Post Hoc Test and the Tuckey Test, that the garlic extract The single most effective used as an anti-bacterial agent is the concentration of 100%, resulting in an average inhibition of 12.81 mm. The ability of single garlic extract to inhibit increases with increasing extract concentrate.
Co-Authors Adityo Nugroho, Adityo Agustiani, Rina Dwi Andika Ristiono Anggraeni, Gita Aniuranti, Asfi Ari Dwi Nurasih Azizah, Teguh Chamid Sutikno Daniel Joko Wahyono Denny Indra Yudhistira Diah Arimbi Diah Harmawati Eko Hendarto Eko Hendarto Eti Wahyuningsih Falah, Muhammad Gita Anggraeni Hendro Pramono Herdian Farisi I Gede Arinton Karseno Khoiriyah, Anna Jarrotul Maditaningtyas Hawwa Zuwanda Mimien Heni Irawati Al Muhdhar, Mimien Heni Irawati Muhammad Falah Nur Laila Rahayu Nura Khabita Nuraini Muhassanah Nurasih, Ari Dwi Oksa Hugi Lestiani Rabbani, Fadhil Rahayu, Nur Laila Rahayu, Nurlaila Rina Dwi Agustiani Rovik, Anwar Sadiah, Hilmi Halimatus Silmi Kafah Siti Fatimah Tien Setyaningtyas Wahyu Maulana Endris Wahyu Siswandari Zain, Nasywa Luthfi Zainul Mustofa, Zainul Zulaikhah, Siti Rahmawati