Claim Missing Document
Check
Articles

Found 30 Documents
Search

KISARAN INANG ISOLAT Colletotrichum gloeosporiodes ASAL MANGGA PADA BEBERAPA PASCAPANEN BUAH Evan Purnama Ramdan; Inti Mulyo Arti; Ummu Kalsum; Putri Irene Kanny
Jurnal Pertanian Presisi (Journal of Precision Agriculture) Vol 6, No 2 (2022): Jurnal Pertanian Presisi
Publisher : Universitas Gunadarma

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.35760/jpp.2022.v6i2.6778

Abstract

Colletotrichum gloesporiodes is one of the species of Colletotrichum that causes anthracnose on various fruits both in the field and postharvest. C. gloesporiodes is a cosmopolitan pathogen so it is important to know which fruits can be infected as a basis for prevention. This study aimed to determine the host range and virulence level of C. gloesporiodes in several postharvest fruits. The research was conducted at the Agrotechnology Intermediate Laboratory, Gunadarma University from March 2022 to April 2022. This study used a completely randomized design consisting of 4 types of fruit (mango, chili, papaya, and tomato) as treatment. Each treatment consisted of 4 units and then repeated 3 times so that there were a total of 48 experimental units. C. gloesporiodes isolate was obtained from mango fruit with anthracnose symptoms. The isolates obtained were inoculated to tomatoes, chilies, papayas, and mangoes as controls. Anthracnose symptoms and lesin diameter were observed at 7 days after inoculation. The diameter of the lesin was measured to determine the level of virulence. The results showed that C. gloesporiodes was able to colonize and infect chilies, papayas, and mangoes as hosts, but there was no inoculated to tomatoes. The ability to colonize and infect is indicated by symptoms in the form of yellow to black spots, sunken, and watery. The virulence category showed different levels in each fruit with a range from very low to moderate. The conclusions obtained from this study were that C. gloesporiodes was able to infect chilies and papayas with different levels of virulence.
Peran Solarisasi Tanah terhadap Pertumbuhan Patogen Tular Tanah dan Populasi Mikroba Tanah Evan Purnama Ramdan; Astri Afriani; Andini Hanif; Cheppy Wati; Nurholis Nurholis; Dwi Astuti; Widodo Widodo
Agrotechnology Research Journal Vol 6, No 1 (2022): Agrotechnology Research Journal
Publisher : Perkumpulan Agroteknologi/Agroekoteknologi Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (331.97 KB) | DOI: 10.20961/agrotechresj.v6i1.55979

Abstract

Soil-borne soil pathogens are pathogens that inhabit the soil and can survive for years in the soil, making them very difficult to control. Control with pesticides and fungicides harms the ecosystem, so other controls are needed such as soil solarization. This study aims to determine the growth response of soil-borne pathogens and soil microbial populations to soil solarization treatment. The study used a completely randomized design with 4 treatments consisting of solarization on soil media, solarization on soil and compost media, without solarization on soil media, and without solarization on soil media and compost on plastic trays. Each treatment was repeated 4 times. The soil-borne pathogens used were Sclerotium rolfsii and Rigidoporus lignisus. Each pathogen was planted in each planting medium with a depth of 5 and 10 cm. Then each tray is covered with 0.1 mm thick transparent plastic. Then given solarization treatment for four weeks. At the end of the observation, sclerotia and R. lignosus were grown on PDA media to be tested for pathogen survival and the solarization efficacy against pathogen growth was calculated. Soil samples from each treatment were also taken to calculate the soil microbial population. The results showed that soil solarization was able to suppress the growth of R. lignosus by 80-100% and S. rolfsii by 100%. Meanwhile, the microbes found in the soil solarization treatment consisted of groups of bacteria and fungi, respectively 7.67×104–1.90×107 CFU.mL-1 and 1.00×104–5.82×105 CFU.mL-1.
Suppression of Xanthomonas oryzae pv. oryzae infection in rice seeds: investigating the optimal temperature and packaging conditions for enhanced pathogen control and seed quality Nikko; Evan Purnama Ramdan; Risnawati; Herik Sugeru
Jurnal Ilmiah Pertanian Vol. 20 No. 2 (2023): Jurnal Ilmiah Pertanian
Publisher : Universitas Lancang Kuning

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.31849/jip.v20i2.13205

Abstract

The pathogen Xanthomonas oryzae pv.oryzae (Xoo) is responsible for rice bacterial leaf blight (BLB), a disease that can significantly reduce rice yield by 60-80%. Xoo can also be transmitted through seeds, making it a seed-borne pathogen. Therefore, it is crucial for farmers to consider environmental factors when storing seeds. This research aims to determine the optimal temperature and packaging for storing rice seeds to prevent Xoo infection. The study employed a split-plot design, where the main plot consisted of two temperature treatments: low (4 ᵒC ± 2 ᵒC) and room temperature (28 ᵒC ± 3 ᵒC). The subplots focused on three packaging types: no packaging as a control, aluminum foil, and polyethylene plastic, resulting in a total of six treatment combinations. Seed pathology testing was conducted using the liquid assay method, while physiological testing utilized the growing on test method. The results revealed that plastic packaging at room temperature provided the most effective treatment for suppressing Xoo, exhibiting the lowest infectivity, number of colonies, and strength vigor index. Conversely, room temperature without packaging demonstrated the highest physiological quality in terms of seed germination and length. However, the temperature and packaging conditions are optimal for the growth of paddy seeds, i.e., at room temperature without packaging.
The Relationship Between Soil Fertility and Basal Stem Rot Disease in Oil Palm Plantations Evan Purnama Ramdan; Arief Hartono; Giyanto Giyanto; Sri Hendrastuti Hidayat; Widodo Widodo
AGROSAINSTEK: Jurnal Ilmu dan Teknologi Pertanian Vol 7 No 1 (2023): AGROSAINSTEK: Jurnal Ilmu dan Teknologi Pertanian
Publisher : Universitas Bangka Belitung

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.33019/agrosainstek.v7i1.384

Abstract

In oil palm, Ganoderma boninense causes stem rot disease, which is often difficult to control, and soil fertility status is related to the ecology of G. boninense as a soil-borne pathogen. Therefore, this study aims to evaluate the relationship between soil fertility and stem rot disease as well as appropriate management methods to control the disease. This was carried out at the Nusantara Plantation Company's 7, Unit Kiwah Rejosari-Pematang from June 2021 to January 2022. The determination of observation blocks was carried out selectively using three blocks of land attacked by Ganoderma boninense with the same criteria for the year of planting and the same soil type. Each block consists of five plots. Each plot consisted of five sub-plots, consisting of 3 oil palms for disease severity assessment and soil sampling. The soil for each subplot was composed of 15 samples, which were analyzed for physical and chemical properties of the soil. Determination of fertility status based on the soil research manual published by the Indonesian Bogor Soil Research Center with parameters from the analysis results. The limiting factor for fertility is the cation exchange capacity of the soil, which ranges from 10.07 meq/100 g to 17.68 meq/100  g, and the C-organic content, which ranges from 0.40 to 1.15%. According to chi-square analysis, this fertility-limiting factor is related to disease severity. Therefore, management needs to be done by adding organic matter to the soil, practicing organic or inorganic fertilization, and following the principles of cultivating healthy oil palm plants.
Dry Heat Treatment and Galangal Rhizome Extract on Soybean Seeds Infected with Xanthomonas axonopodis: Dry Heat Treatment and Galangal Rhizome Extract on Soybean Seeds Infected with Xanthomonas axonopodis Fauziyah, Qonitah; Risnawati, Risnawati; Yulianti, Fitri; Ramdan, Evan Purnama
Jurnal Fitopatologi Indonesia Vol. 20 No. 1 (2024): Januari 2024
Publisher : The Indonesian Phytopathological Society (Perhimpunan Fitopatologi Indonesia)

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.14692/jfi.20.1.15-23

Abstract

Kedelai (Glycine max) adalah salah satu sumber protein nabati yang kebutuhannya terus meningkat. Salah satu kendala utama pada budi daya kedelai ialah penyakit tular benih membisul oleh bakteri Xanthomonas axonopodis pv. glycine. Penelitian ini bertujuan mengevaluasi perlakuan panas kering dan ekstrak rimpang lengkuas terhadap penekanan populasi X. axonopodis pv. glycine dan vigor benih kedelai yang terinfeksi bakteri. Penelitian disusun dalam rancangan acak lengkap. Percobaan pertama ialah perlakuan panas kering pada suhu 45 ℃ dengan waktu pemanasan 6, 12, dan 24 jam, serta kontrol tanpa pemanasan. Percobaan kedua ialah perendaman biji selama satu jam di dalam ekstrak rimpang lengkuas dengan konsentrasi 25%, 20%, dan 15%, serta kontrol (air). Peubah yang diamati ialah jumlah populasi bakteri, daya kecambah, vigor, dan viabilitas benih. Hasil penelitian menunjukkan bahwa perlakuan panas dan ekstrak rimpang lengkuas berpengaruh nyata terhadap penurunan populasi X. axonopodis terbawa benih kedelai. Perlakuan benih kedelai dengan panas kering selama 24 jam menyebabkan benih bebas dari X. axonopodis, tetapi perkecambahan dan vigor benih mengalami penurunan. Sementara perlakuan benih dengan ekstrak rimpang lengkuas 25% menyebabkan populasi X. axonopodis menurun hingga 7.0 × 102 cfu mL-1, dengan tidak memengaruhi vigor dan viabilitas kedelai. Hasil penelitian mengindikasikan bahwa perlakuan panas kering memiliki potensi lebih baik dibandingkan dengan ekstrak rimpang lengkuas dalam mengeleminasi X. axonopodis dalam benih kedelai. Namun, karena perlakuan panas memiliki dampak negatif terhadap perkecambahan benih maka perlu dicari suhu dan waktu pemanasan yang optimal untuk menekan populasi X. axonopodis tanpa mengurangi viabilitas benih.
RESPON PERTUMBUHAN BIBIT KELAPA SAWIT DAN PENYAKIT BUSUK PANGKAL BATANG TERHADAP APLIKASI AGENS MIKROBA RHIZOFER Salsabila, Amelia; Budiman, Budiman; Risnawati, Risnawati; Ramdan, Evan Purnama
Jurnal Pertanian Presisi (Journal of Precision Agriculture) Vol 8, No 1 (2024)
Publisher : Universitas Gunadarma

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.35760/jpp.2024.v8i1.7989

Abstract

Penyakit busuk pangkal batang yang diakibatkan oleh G. boninense merupakan salah satu hambatan pada perkebunan kelapa sawit yang telah dikendalikan melalui pengendalian teknis, hayati dan kimiawi. Rizosfer ialah wilayah yang sempurna untuk pertumbuhan dan perkembangan mikroba tanah, termasuk di dalamnya agens pengendalian hayati. Penelitian ini bertujuan untuk mengetahui pengaruh aplikasi agens hayati dari daerah perakaran tanaman kelapa sawit terhadap penekanan penyakit busuk pangkal batang dan komponen pertumbuhan tanaman kelapa sawit. Penelitian ini dilaksanakan dengan menggunakan Rancangan Acak Kelompok. Terdapat 4 perlakuan dengan 6 ulangan, setiap petak percobaan terdiri dari 3 tanaman, sehingga jumlah keseluruhan sampel sebanyak 72 unit percobaan. Parameter yang digunakan pada penelitian ini adalah pertumbuhan tanaman kelapa sawit (tinggi tanaman, jumlah daun, diameter pangkal batang, jumlah akar, panjang akar) dan gejala penyakit busuk pangkal batang. Hasil penelitian menunjukkan bahwa pengaplikasian 3 jenis isolat (agens hayati isolat no. 3 bakteri, agens hayati isolat no. 15 bakteri, dan agens hayati isolat no. 1 cendawan) mampu menekan perkembangan dan penyebaran G. boninense serta meningkatkan pertumbuhan tanaman kelapa sawit. Perlakuan P2 (agens hayati isolat no. 3 bakteri) memberikan pengaruh terbaik dibanding perlakuan lainnya.
Identification, Pathogenesis and Virulence Test of Fungus Causes Postharvest Disease of Gedong Gincu Mango from Pal Market, Cimanggis, Depok Arti, Inti Mulyo; Asnur, Paranita; Kurniasih, Ratih; Ramdan, Evan Purnama
JURNAL PEMBELAJARAN DAN BIOLOGI NUKLEUS Vol 8, No 2: Jurnal Pembelajaran Dan Biologi Nukleus Juli 2022
Publisher : Fakultas Keguruan dan Ilmu Pendidikan Universitas Labuhanbatu

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.36987/jpbn.v8i2.2628

Abstract

Mango is a climacteric fruit that can increase in maturity after harvest. As a result, mangoes will rot more easily because they are infected with pathogens such as fungi. The objectives of the study were to identify, examine the pathogenicity, and virulence of the fungus that causes postharvest disease in mangoes. Sampling of mangoes was carried out at the Pal market, Cimanggis, Depok by selecting fruits that were indicated to be infected with post-harvest diseases. Symptoms that appear are then described visually. Isolation of the fungus was carried out from the part of the mango fruit on Potato Dextrose Agar (PDA) media, then it was identified morphologically both macroscopically and microscopically. The virulence test was carried out by inoculating the fungus at 4 inoculation points of mango fruit. The results showed that the symptoms that appeared were symptoms of anthracnose in the form of blackish brown, sunken, and widespread spots on the mango skin. The results of morphological identification showed that the associated fungus was Colletotrichum gloeosporiodes which was characterized by white fungal colonies and fast growth, while the condia were cylindrical in shape with rounded ends. Confirmation results with Koch's postulates showed that postharvest anthracnose in mangoes was caused by C. gloeosporiodes. Meanwhile, virulence testing showed that C. gloeosporiodes had moderate to high virulence levels with symptomatic lesions ranging from 2,50 to 5,85 cm.
INTENSITAS PENYAKIT ANTRAKNOSA CABAI PADA PERLAKUAN PGPR DAN POTENSI SENSOR TERMAL UNTUK DETEKSI DINI Amalia, Khafidah; Ramdan, Evan Purnama; Arti, Inti Mulyo
Jurnal Pertanian Presisi (Journal of Precision Agriculture) Vol 8, No 2 (2024)
Publisher : Universitas Gunadarma

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.35760/jpp.2024.v8i2.11526

Abstract

Chilli peppers are easily infected with anthracnose disease caused by Colletotrichum gloeosporioides. Early detection, such as temperature changes with thermal sensors before visual symptoms appear, can prevent developing more severe diseases. This study aims to evaluate the potential of anthracnose detection using thermal sensors in chilli and the intensity of anthracnose disease in applying Plant Growth Promoting Rhizobacteria (PGPR). This study used a completely randomized complete group design (RKLT). PGPR was applied twice at the seedling preparation stage and at the plant age 30 days after planting (HST). Seeds that are ready to be sown are soaked first with 3 dose treatments, namely; without pgpr as control (P0), PGPR 25 g/5 L sterile distilled water (P1) and 50 g/5 L sterile distilled water (P2). Apart from that, PGPR application was also carried out on plants aged 30 HST with no PGPR as a control (P0), a dose of 100 mL PGPR/plant (P1), 200 mL PGPR/plant (P2). The results showed that the thermal sensor has the potential as an early detection method of anthracnose disease, as seen from the difference in the temperature of chilli fruit pre-inoculation with post-inoculation by 2 - 3ºC. In addition, PGPR treatment was able to suppress anthracnose disease with the lowest disease incidence of 19,33% (P1), disease severity of 16,56% (P2) and AUDPC of 49,38 units (P1).
PERBEDAAN INTENSITAS NAUNGAN DAN VARIETAS TERHADAP SERANGAN HAMA DAN PENYAKIT PADA TANAMAN STROBERI (Fragaria L) Istianah, Istianah; Kalsum, Ummu; Ramdan, Evan Purnama; Warip, Warip
Jurnal Pertanian Presisi (Journal of Precision Agriculture) Vol 9, No 1 (2025)
Publisher : Universitas Gunadarma

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.35760/jpp.2025.v9i1.13037

Abstract

Pengembangan stroberi di dataran rendah Indonesia masih terbatas, tetapi bisa menjadi peluang untuk memperluas area budidaya stroberi. Budidaya stroberi di dataran rendah akan menghadapi tantangan baru, terutama serangan hama dan penyakit. Penelitian ini bertujuan untuk mengetahui pengaruh perbedaan intensitas naungan dan varietas terhadap serangan hama dan penyakit tanaman stroberi di dataran rendah. Penelitian ini menggunakan Rancangan Kelompok Lengkap Teracak (RKLT) Tersarang dengan dua faktor. Faktor utama terdiri atas empat taraf yaitu kontrol (P3), naungan 55% (P1), naungan 65% (P2), dan naungan 75% (P4). Faktor tersarang terdiri atas tiga varietas stroberi, yaitu varietas California (R1), varietas Mencir (R2), dan varietas Sweet Charlie (R3). Dengan demikian, terdapat 12 kombinasi perlakuan dengan 4 ulangan, masing-masing ulangan terdiri atas 3 sampel tanaman sehingga diperoleh total 144 tanaman. Hasil menunjukkan bahwa hama yang menyerang tanaman stroberi meliputi ulat grayak, ulat penggulung daun, belalang, kutu putih, sedangkan penyakit yang menyerang meliputi embun tepung, dan busuk buah. Naungan 75% meningkatkan kerusakan daun tertinggi akibat hama pada kontrol sebesar 8.2%, varietas Mencir lebih rentan akibat kerusakan buah dibanding Sweet Charlie sebesar 0.14%. Terdapat korelasi positif antara intensitas cahaya dan suhu udara terhadap intensitas serangan hama dan penyakit, namun kelembaban udara menunjukkan korelasi negatif dengan serangan hama dan penyakit.
The Ability of Several Biological Agents to Induce Resistance of Rice from Pyricularia oryzae Attacks In Vitro and In Vivo: Kemampuan Beberapa Agens Hayati dalam Menginduksi Ketahanan Tanaman Padi dari Serangan Pyricularia oryzae secara In Vivo dan In Vitro Lestari, Shyntiya Ayu; Ramdan, Evan Purnama; Risnawati, Risnawati; Pribadi, Edi Minjai
Jurnal Proteksi Tanaman (Journal of Plant Protection) Vol. 6 No. 1 (2022): June 2022
Publisher : Plant Protection Department, Faculty of Agriculture, Universitas Andalas

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.25077/jpt.6.1.1-12.2022

Abstract

Rice (Oryza sativa Linnaeus) is the main food crop that is needed daily for the majority of Indonesian people. One of the diseases that increase rice production is the attack of pests and other diseases caused by Pyricularia oryzae. Biological agents are an alternative to control this disease. Therefore, the aim of this study was to determine the effectiveness of the induction of rice plant resistance using several biological agents against blast disease. The study was carried out in 2 stages, the first in vivo using a Randomized Block Design consisting of 4 biological agent treatments, namely Trichoderma sp., Bacillus subtilis, Pseudomonas fluorescens and sterile Aquades (control) which was repeated 6 times with each replication consisted of 3 plant samples so that the total number of samples observed was 72 plant samples. The second stage was carried out in vitro by inoculating P. oryzae on 3 pieces of rice plant leaves as a result of in vivo experiments. The design used was a completely randomized design with 4 agent treatments which were repeated 6 times. The results showed that the induction of rice plant resistance with P. fluorescens was able to reduce the severity of disease caused by P. oryzae attack (72.22%), with a decrease in efficacy of 25.48%, and the lowest AUDPC (691.66). In general, the resistance induction did not affect rice growth, but Trichoderma sp and P. fluorescens were able to increase the grain weight produced (3.11 and 2.80 g per plant, respectively).