Garuda - Garba Rujukan Digital

p-Index From 2021 - 2026

2.083

P-Index

This Author published in this journals

All Journal HAYATI Journal of Biosciences Jurnal Gizi dan Pangan Jurnal Ilmu Pertanian Indonesia ACTA VETERINARIA INDONESIANA Hemera Zoa Buletin Peternakan ILMU KELAUTAN: Indonesian Journal of Marine Sciences Jurnal Ilmu-Ilmu Peternakan (Indonesian Journal of Animal Science) Indonesian Journal of Biotechnology Jurnal Riset Veteriner Indonesia (Journal of The Indonesian Veterinary Research) Jurnal Medik Veteriner ARSHI Veterinary Letters Jurnal Kedokteran Hewan

Mokhamad Fahrudin

Departemen Anatomi Fisiologi Dan Farmakologi, Fakultas Kedokteran Hewan, Institut Pertanian Bogor, Jln Agathis, Dramaga, Bogor, Jawa Barat, Indonesia, 16680

Author-ID : 262930

Agriculture, Biological Sciences & Forestry Biochemistry, Genetics & Molecular Biology Earth & Planetary Sciences Health Professions Immunology & microbiology Materials Science & Nanotechnology Medicine & Pharmacology Veterinary Other

Published : 28 Documents Claim Missing Document

Claim Missing Document

Articles

1 2 3

Analisis Proteomik Cairan Sinovial Sendi Domba: Efektivitas Metode dan Profil Protein Fungsional Kusdiantoro Mohamad; Wiwit Ridhani Rahmaniyah; I Ketut Mudite Adnyane; Mokhamad Fahrudin; Arief Boediono
Acta VETERINARIA Indonesiana Vol. 8 No. 2 (2020): Juli 2020
Publisher : IPB University

Penelitian ini bertujuan untuk mengevaluasi metode deplesi dan digesi protein, serta menganalisis proteom cairan sinovial (SF) sendi pada domba sehat menggunakan kromatografi cair–tandem spektrometri massa. Cairan SF dikoleksi dari enam ekor domba garut betina, umur ±4 tahun, berat 35-40 kg, dan sehat secara klinis. Deplesi protein berkelimpahan tinggi dilakukan dengan metode spin column menggunakan TOP 12 dan metode proteospin, sementara digesi protein dengan tiga metode, yaitu digesi in solution, in gel, dan in-solution + filter-aided sample preparation (FASP). Metode yang terbaik kemudian dilanjutkan dengan fraksinasi peptida menggunakan ultra performance liquid chromatography. Metode deplesi proteospin dengan digesi protein in-solution + FASP merupakan metode terbaik berdasarkan nilai coverage dan sequest HT. Hasil analisis proteomik terkarakterisasi 52 protein pada database spesies domba. Anotasi gen ontologi menggunakan DAVID analysis menunjukkan bahwa protein-protein SF tersebut merupakan komponen sel terutama sebagai eksosom ekstraseluler, fungsi molekuler sebagai aktivitas inhibitor endopeptidase tipe-serin dan pengikat ion kalsium; serta proses biologis sebagai angiogenesis dan koagulasi darah atau pembentukan fibrin. Analisis KEGG pathway menunjukkan protein SF berperan utama pada jalur kaskade koagulasi dan komplemen.

PCS-1 Development of Mouse Parthenogenetic Embryos in Phosphate Free Medium Vista Budiariati; Dwi Budiono; Mokhamad Fahrudin; Berry Juliandi; Ratih Rinendyaputri; Arief Boediono
Hemera Zoa Proceedings of the 20th FAVA & the 15th KIVNAS PDHI 2018
Publisher : Hemera Zoa

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (516.416 KB)

Parthenogenesis is an artificial oocytes activation process without paternal contribution so that embryos will develop without fertilization [3]. The process of parthenogenesis as a reproductive strategy occurs in species of insect, pisces, or amphibian, which not require any implantation. Naturally, parthenogenesis is not common in mammals, but by understanding cellular mechanism during fertilization, it is possible to artificially activate mammalian oocytes.Blastocyst, derived from parthenogenesis, can be used for developmental study, embryo reconstruction, and one of potential source for pluripotent stem cells. Unfortunately, previous studies reported that parthenogenetic embryo did not achieve exhilarating blastocyst rate.One of the component that has been predicted to inhibit parthenogenetic embryo development is phosphate. Haraguchi et al. (1996) reported that phosphate caused a negative effect on in vitro culture of AKR/N mice fertilized embryos, removal of phosphate elements was significantly improved the blastocyst rate up to 42.6% [1]. The effects of phosphate also became an interesting finding in the study that reported mouse fertilized embryos could well developed in modified medium rat 1 cell embryo medium (MR1ECM) which not contained any phosphate [2].The effect of phosphate on in vitro culture of mouse parthenogenetic embryo has not been clear. The aim of this research was to analyze inhibitory effect caused by phosphate in the medium and compare the development pattern between parthenogenetic and fertilized embryos in order to reach optimal production of parthenogenetic blastocyst for further purposes.

PF-17 The Development of Crude Testicular Cells in In Vitro Culture Wahono Esthi Prasetyaningtyas; Ni Wayan Kurniani Karja; Srihadi Agungpriyono; Mokhamad Fahrudin
Hemera Zoa Proceedings of the 20th FAVA & the 15th KIVNAS PDHI 2018
Publisher : Hemera Zoa

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (488.568 KB)

Spermatogenesis is a continuous process in which spermatogonial stem cells (SSC) develop into specific germ cells before terminally differentiating to form spermatozoa. The process is supported by Sertoli cells, which are in close contact with germ cells in the seminiferous tubules. Sertoli cells provide essential hormonal signals, nutrients, and physical support to germ cells for successful spermatogenesis.The crude testicular cells (CTC) contains many cell types, like Sertoli cell, Leydig cell, spermatogonial stem cell (SSC), spermatocyte and other testicular somatic cells (Shah et all. 2016). Testicular cells are believed to secrete various growth factors that induced the spermatogenesis process. The spermatogonial stem cells are unique population of cells in the male testis, which dual function. First self-renewing their population to maintain the number of stem cells, secondary function is differentiating into spermatids in testis (Wang et al. 2015).Spermatogenic cells differentiation needed the similar microenvironment in vivo spermatogenesis. The essential nutrients was collected from healty culture and the culture contained mixed population of cells both the somatic cells and spermatogenic cells. To identification the spermatogenic cells using Periodic Acid Schifft (PAS) staining (Chang et al. 2011). The present study examined the development of crude testicular cells using PAS staining.

Relationship between Nucleus Swelling and Development Competence of Bovine Cloned Embryos Reconstructed by Enucleated Oocytes with Serum-starved or Serum-fed Fetal Somatic Cells Mokhamad Fahrudin; Ni Wayan Kurniani Karja; Tatsuyuki Suzuki
Indonesian Journal of Biotechnology Vol 10, No 2 (2005)
Publisher : Universitas Gadjah Mada

This study was conducted to examine the occurrence of nuclear remodeling (nucleus swelling) and its effects on the subsequent in vitro development of bovine embryos reconstructed by serum-starved and serum-fed somatic cells. Results from this study demonstrated that all of the reconstructed embryos that received serum-starved and serum-fed somatic cells exhibited condensed-nuclei. More than 90% of the transferred nuclei exhibited nuclear envelope breakdown and premature chromatin condensation which clearly distinct from an intact nucleus. There was no significant difference on the degree of nucleus swelling in SS-NT embryos or SF-NT embryos, indicating that either serum-starved or confluent somatic cell lines could be reprogrammed by the recipient cytoplasm environments in similar pattern. Although the fusion rate was not significantly different among the groups, the proportion of SS-NT embryos which developed to the 2- to 4-cell stage (89.7%) and to the 8- to 16-cell stage (74.7%) was significantly higher than that of SF-NT embryos. Whereas, the proportion of reconstructed embryos that developed to the morula and blastocyst stages were not significantly different among the groups. Results of these studies demonstrate that reconstructed embryos, which received either serum-starved or serum-fed confluent somatic cells, showed similar developmental competence to the blastocyst stage.

Developmental Competence of Early Stage Porcine Embryos Cultured in Medium with Different Energy Substrate in vitro Ni Wayan Kurniani Karja; Kazuhiro Kikuchi; Mokhamad Fahrudin
Indonesian Journal of Biotechnology Vol 11, No 1 (2006)
Publisher : Universitas Gadjah Mada

To elucidate the effect of energy requirement during the early embryonic development on their developmental ability to develop to blastocyst stage, in vitro fertilized (IVF) porcine one-cell embryos were cultured in modified North Carolina State University (NCSU)-37 supplemented with different energy substrate. Result indicated that the cleavage rate of embryos in Pyr-Lac and Gluc-Pyr-Lact groups was significantly higher than in those in Gluc group and Gluc-Rib group (P < 0.05). At Day 6 of culture, the highest proportion of embryos develop to the blastocyst stage was obtained in the presence of pyruvate-lactate only. In the medium with glucose, the addition of pyruvate-lactate or ribose slightly increased the proportion of embryos develop to the blastocyst stage, however the value were not significantly different form those obtained in the presence of glucose only. The mean cell number in blastocysts derived from Pyr-Lac and Gluc-Pyr-Lact groups were significantly higher than those in the Gluc group (P < 0.05). These results indicated that the presence of glucose only, as energy substrate, during the first 2 days of in vitro culture (IVC) caused a decrease in development of in vitro produced (IVP) porcine embryos to the blastocyst stage and mean cell number in blastocysts.

Temperature of Eggshell, Weight Loss, and Air Sac on Hatched Local Duck Eggs During Incubation Yusuf Kurniawan; Rukmiasih Rukmiasih; Mokhamad Fahrudin; Peni Soeprapti Hardjosworo
Buletin Peternakan Vol 42, No 3 (2018): BULETIN PETERNAKAN VOL. 42 (3) AUGUST 2018
Publisher : Faculty of Animal Science, Universitas Gadjah Mada

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.21059/buletinpeternak.v42i3.31279

The study was conducted to analyze the characteristic of eggs (temperature of eggshell, weight loss, and air sac alteration) at various hatching period of local duck hatched (H) and unhatched (UH) at the final observation, and to find out the effective time for estimating the life of embryo during the end of incubation. A total of 146 eggs were incubated and observed between 1 and 25 days of incubation (DOI). The results of weight loss and air sac change showed a significant difference (P<0,05) between H and UH eggs on 7 to 25 DOI, while the temperature of eggshell was only different on 25 DOI. The average characteristic of H group (temperature of eggshell, weight loss, and air sac alteration) on 25 DOI was recorded 38,46oC, 11,84%, and 51,03%, respectively. It can be concluded that 3 characteristics of eggs influence hatchability of local duck. Weight loss and air sac alteration parameters can be applyed to estimate the hatched eggs between 7 and 25 DOI, but the temperature of eggshell can be administrated after 25 DOI.

PERKEMBANGAN EMBRIO SAPI SETELAH FERTILISASI MENGGUNAKAN METODE INTRACYTOPLASMIC SPERM INJECTION (ICSI) DAN AKTIVASI DENGAN STRONTIUM Muhammad Gunawan; Mokhamad Fahrudin; Arief Boediono
Jurnal Kedokteran Hewan Vol 8, No 2 (2014): September
Publisher : Universitas Syiah Kuala

Penelitian ini bertujuan mengetahui pengaruh kombinasi intracytoplasmic sperm injection (ICSI) dan aktivasi dengan strontium untuk mengetahui perkembangan pronukleus dan perkembangan embrio sampai tahap blastosis. Kombinasi ICSI dan strontium 20 mM meningkatkan perkembangan pronukleus 2-PN mencapai 43,59%. Hasil perkembangan embrio pada perlakuan kombinasi ICSI dan strontium 20 mM mencapai tingkat perkembangan 2-4 sel (50,5%), 8-16 sel (43,73%), dan blastosis (15,63%). Kesimpulan dari hasil penelitian ini adalah kombinasi ICSI dengan aktivasi strontium 20 mM mampu menghasilkan perkembangan in vitro embrio sapi yang lebih baik.

The Quality of Hatching Egg and Development of Embryonic Somites in Kampung and Sentul Chickens Zulfikar, Muhammad Irsyad; Ulfah, Maria; Fahrudin, Mokhamad
Jurnal Ilmu-Ilmu Peternakan Vol. 34 No. 3 (2024): December 2024
Publisher : Faculty of Animal Science, Universitas Brawijaya

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.21776/ub.jiip.2024.034.03.14

Kampung and Sentul chickens are Indonesian local chicken breeds that are widely developed by breeding companies as strains for meat production. The genetic differences among these chicken strains can impact the quality of the hatching eggs they produce. Somite development is a crucial part of chicken embryo growth during the hatching process, as it precedes the formation of vertebrae and skeletal muscles, ultimately affecting chick quality. This study aimed to analyse how both strains of hens affect hatching egg quality and somite development. The sample included 126 hatching eggs, with 63 from Kampung chickens and 63 from Sentul chickens. The observed parameters of hatching egg quality included egg weight, egg shape index, eggshell thickness, eggshell weight, eggshell strength, albumen index, yolk index, yolk color score, Haugh Unit (HU), and embryo development indicators, such as the development of the primitive streak and progression of somite number. The data were analysed using a t test. Differences between chicken strains significantly affected (P<0.05) egg weight, shell thickness, the albumen index, and HU. The different strains also showed significant differences (P<0.05) in embryo somite development, with the primitive streak development of the Sentul chicken being slower than that of the Kampung chicken. In conclusion, Kampung chickens produce hatching eggs with superior interior quality, primitive streak development and somite number compared to the hatching eggs of other chickens. However, Sentul chickens also demonstrate good exterior egg quality, as shown by their egg weight and shell thickness.

The Quality of Indo-Pacific Bottlenose Dolphin (Tursiops aduncus) Sperm Following Liquid-storage in Low Temperature Fahrudin, Mokhamad; Gusdinar, Rizal; Arifiantini, Raden Iis; Prasetyaningtyas, Wahono Esthi; Adnyane, I Ketut Mudite; Elmanaviean, Muhammad; Nugraha, Arifin Budiman; Karja, Ni Wayan Kurniani
ILMU KELAUTAN: Indonesian Journal of Marine Sciences Vol 29, No 4 (2024): Ilmu Kelautan
Publisher : Marine Science Department Diponegoro University

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.14710/ik.ijms.29.4.456-466

Indo-Pacific bottlenose dolphin (Tursiops aduncus) is a marine mammal that lives in relatively small populations. The geographic ranges of this species are susceptible to the effects of human activities, thereby necessitating conservation efforts to prevent extinction. Therefore, this study aimed to evaluate the daily quality of dolphin sperm after several days of refrigeration. The sperm of two male dolphins were stored at 4oC for 4 days, and the quality was observed daily to determine the motility, viability, membrane integrity, and sperm abnormalities. Sperm samples were divided into four groups, consisting of two centrifuged followed by the removal of seminal plasma, and two groups without centrifugation, containing 100x106 and 200x106 sperm/ml each. After liquid storage, the motility of sperm was 63-75% with no significant reduction in the first 3 days. Sperm viability following storage was 65-75% and the percentage with abnormal morphology ranged from 2-6%. Furthermore, there was no significant increase in abnormal morphology of sperm on any day of storage for 3 days. Sperm membrane integrity was 36-49%, with no significant reduction in the membrane integrity in the first 2 days. There was no significant difference in sperm quality, although centrifugation and removal of seminal plasm had a slight effect. The results of this study showed that Indo-Pacific bottlenose dolphin sperm could be stored for a short period as liquid storage while maintaining a quality that allows for future use.

Quality of Chilled Ram Semen in Tris Egg Yolk Extender Added with Different Concentrations of Glutamine: Quality of Chilled Ram semen in extender added with glutamine Aisyah Fidela Siregar; Mokhamad Fahrudin; Ni Wayan Kurniani Karja; Wahono Esthi Prasetyaningtyas
Jurnal Riset Veteriner Indonesia (Journal of The Indonesian Veterinary Research) VOLUME 7, No 2, JULY 2023
Publisher : Hasanuddin University

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20956/jrvi.v7i2.22034

Glutamine is a non-essential amino acid that can reduce free radicals and act as an antioxidant. This study aims to determine the effect of adding glutamine in egg yolk tris diluent at concentrations of 0 mM (K), 2,5 mM (G2,5), 5 mM (G5), and 7,5 mM (G7,5) on the quality of liquid semen and kept in a refrigerator at 4 °C. Microscopic evaluation was performed immediately after dilution (H0) and every 24 hours until day 5 (H5). The results showed that the G 7,5 mM group could maintain better motility and viability (P < 0,05) than the K, G 2,5, and G 5 groups. The addition of glutamine did not have a significant effect (P> 0.05) in maintaining the MPU of spermatozoa. Glutamine with a concentration of 7.5 mM is the best concentration to maintain the quality of sheep's liquid semen.

Co-Authors Aisyah Fidela Siregar Al Mukhlas Fikri Alfiah, Elma Alkaustariyah Lubis Almanda, Wisnu Rahmadan Arief Boediono Berry Juliandi Boenjamin Setiawan Budiariati, Vista Cece Sumantri Diah Nugrahani Pristihadi Dwi Budiono El Zamzami, Arfah Herawana Elmanaviean, Muhammad Gusdinar, Rizal Hardinsyah Harry Murti Harry Murti Ita Djuwita Katrin Roosita Kazuhiro Kikuchi Kazuhiro Kikuchi KAZUHIRO KIKUCHI Ketut Adnyane Mudite Kombo, Muhammad Piter Kusdiantoro Mohamad Lubis, Andri Maruli Tua M Agus Setiadi M. Haviz Makhroja, Lalu Faris Naufal Maria Ulfah Muhamad Rizal Martua Damanik Muhammad Gunawan Muhammad Gunawan Muhammad Rosyid Ridlo Ni Wayan Kurniani Karja Noer Muhammad Dliyaul Haq Nugraha, Arifin Budiman Nurhidayat Nurhidayat Nurwati, Yuni Nur’aisyah Amrah Safitri Pattikawa, Vapriel Andhika Peni Soeprapti Hardjosworo R. Iis Arifiantini Rahmaniyah, Wiwit Ridhani Rakhmawati, Handina Ratih Rinendyaputri Ratih Rinendyaputri Ratih Rinendyaputri Rini Widyastuti Rukmiasih Rukmiasih Safika S, Safika Situmeang, Adrian Sri Anna Marliyati Srihadi Agungpriyono Tatsuyuki Suzuki Tatsuyuki Suzuki, Tatsuyuki Triyaningrum, Triyaningrum Vetnizah Juniantito Wahono Esthi Prasetyaningtyas Wahono Esthi Prasetyaningtyas Wahono Esthi Prasetyaningtyas Wiwit Ridhani Rahmaniyah Yusuf Kurniawan Zulfikar, Muhammad Irsyad

Title

Found 28 Documents
Search

Abstract

Abstract

Abstract

Abstract

Abstract

Abstract

Abstract

Abstract

Abstract

Abstract

Title Search

Found 28 Documents Search

Abstract

Abstract

Abstract

Abstract

Abstract

Abstract

Abstract

Abstract

Abstract

Abstract

Title

Found 28 Documents
Search