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All Journal HAYATI Journal of Biosciences Jurnal Gizi dan Pangan Jurnal Ilmu Pertanian Indonesia ACTA VETERINARIA INDONESIANA Hemera Zoa Buletin Peternakan Jurnal Veteriner ILMU KELAUTAN: Indonesian Journal of Marine Sciences Jurnal Ilmu-Ilmu Peternakan (Indonesian Journal of Animal Science) Indonesian Journal of Biotechnology Jurnal Riset Veteriner Indonesia (Journal of The Indonesian Veterinary Research) Jurnal Medik Veteriner ARSHI Veterinary Letters Jurnal Kedokteran Hewan
Mokhamad Fahrudin
Departemen Anatomi Fisiologi Dan Farmakologi, Fakultas Kedokteran Hewan, Institut Pertanian Bogor, Jln Agathis, Dramaga, Bogor, Jawa Barat, Indonesia, 16680
Agriculture, Biological Sciences & Forestry Biochemistry, Genetics & Molecular Biology Earth & Planetary Sciences Health Professions Immunology & microbiology Materials Science & Nanotechnology Medicine & Pharmacology Veterinary Other

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PERKEMBANGAN EMBRIO SAPI SETELAH FERTILISASI MENGGUNAKAN METODE INTRACYTOPLASMIC SPERM INJECTION (ICSI) DAN AKTIVASI DENGAN STRONTIUM Gunawan, Muhammad; Fahrudin, Mokhamad; Boediono, Arief
Jurnal Kedokteran Hewan Vol 8, No 2 (2014): September
Publisher : Universitas Syiah Kuala

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.21157/j.ked.hewan.v8i2.2651

Abstract

Penelitian ini bertujuan mengetahui pengaruh kombinasi intracytoplasmic sperm injection (ICSI) dan aktivasi dengan strontium untuk mengetahui perkembangan pronukleus dan perkembangan embrio sampai tahap blastosis. Kombinasi ICSI dan strontium 20 mM meningkatkan perkembangan pronukleus 2-PN mencapai 43,59%. Hasil perkembangan embrio pada perlakuan kombinasi ICSI dan strontium 20 mM mencapai tingkat perkembangan 2-4 sel (50,5%), 8-16 sel (43,73%), dan blastosis (15,63%). Kesimpulan dari hasil penelitian ini adalah kombinasi ICSI dengan aktivasi strontium 20 mM mampu menghasilkan perkembangan in vitro embrio sapi yang lebih baik.
The safety evaluation of ethanol extract of torbangun leaves (Coleus amboinicus Lour.) on mouse fetal development Alfiah, Elma; Damanik, Muhammad Rizal Martua; Roosita, Katrin; Fahrudin, Mokhamad
Jurnal Gizi dan Pangan Vol. 13 No. 1 (2018)
Publisher : The Food and Nutrition Society of Indonesia in collaboration with the Department of Community Nutrition, IPB University

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (4631.317 KB) | DOI: 10.25182/jgp.2018.13.1.27-32

Abstract

The purpose of this study was to evaluate the safety of ethanol extract from torbangun leaves on mouse fetal development. This research used 24 female pregnant mouse. The leaves extract was administered orally at dose level of 0; 0.56; 1.68 and 3.36 g/kg body weight. The extract was given from the first day until the eighteenth day of pregnancy period. The ethanol extract of Torbangun leaves contains flavonoids, saponins, tannins, and steroids. The result showed that consumption of 3.36 g/kg torbangun leaves extract had led to significant differences in the decrease of maternal weight change, number of live fetuses, fetal weight, and fetal length. Consumption of Torbangun leaves extract during pregnancy should be avoided due to its potency to interfere the fetal development.  
Identifikasi bakteri enterik gram-negatif dan pola resistensi antimikroba pada burung psittacine yang dipelihara di Indonesia El Zamzami, Arfah Herawana; Safika; Fahrudin, Mokhamad; Kombo, Muhammad Piter; Almanda, Wisnu Rahmadan
ARSHI Veterinary Letters Vol. 9 No. 4 (2025): ARSHI Veterinary Letters - November 2025
Publisher : School of Veterinary Medicine and Biomedical Sciences, Bogor Agricultural University

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.29244/avl.9.4.111-112

Abstract

Psittacines are vulnerable to bacterial diseases, including gram-negative infections. A key challenge in treating these birds is antibiotic resistance, where antibiotics become ineffective against the bacteria. This study aimed to identify gram-negative bacteria present in 11 fecal samples of psittacine from Taman Mini Indonesia Indah (TMII) in East Jakarta, as well as to determine the presence of antibiotic resistance in these bacteria. In this study, bacteria were isolated using MacConkey Agar media, followed by Gram staining and biochemical tests for bacterial identification. Antibiotic resistance testing was conducted using the Kirby-Bauer method against seven antibiotics. The results of bacterial identification showed the presence of Klebsiella sp., Proteus mirabilis, and Escherichia coli in the fecal samples of Psittacine from TMII. Resistance to the antibiotics nalidixic acid, doxycycline, tetracycline, gentamicin, erythromycin, cefotaxime, and trimethoprim-sulfamethoxazole was observed, with trimethoprim-sulfamethoxazole demonstrating the lowest resistance rate among fecal Psittacine bacteria.
Short-term treatment of osteoarthritis-induced sheep with human umbilical cord–derived mesenchymal stem cells: A morphological and proteomic analysis Mohamad, Kusdiantoro; Situmeang, Adrian; Rakhmawati, Handina; Adnyane, I Ketut Mudite; Fahrudin, Mokhamad; Nurhidayat, Nurhidayat; Murti, Harry; Lubis, Andri Maruli Tua; Boediono, Arief
Jurnal Medik Veteriner Vol. 9 No. 1 (2026): April
Publisher : Universitas Airlangga

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20473/jmv.vol9.iss1.2026.106-123

Abstract

Knee joints are the primary sites affected by osteoarthritis (OA) in humans and animals. Although intra-articular human umbilical cord–derived mesenchymal stem cells (hUC-MSCs) show therapeutic promise, their combined use with hyaluronic acid (HA) remains underexplored in large animals. This study evaluated morphological and proteomic changes in a sheep osteoarthritis model treated with both agents. A total of twelve sheep were divided into four groups (n = 3 per group): control (normal and OA) and three treatment groups receiving hUC-MSCs, HA, or a combination of HA and hUC-MSCs (HA + hUC-MSCs). OA was induced by meniscectomy, and intra-articular injections were administered four weeks post-surgery. Macroscopic scoring was conducted according to the Osteoarthritis Research Society International system, while histological evaluation was performed using hematoxylin–eosin, Safranin O, and Masson’s trichrome staining. Proteomic analysis of synovial fluid was carried out using liquid chromatography–tandem mass spectrometry. The macroscopic OA score in the HA + hUC-MSC group was lower than in the other treatment groups and was not significantly different from the normal group (p > 0.05). Microscopic observations indicated that hUC-MSCs supported joint cartilage regeneration by preserving proteoglycan and collagen content. Proteomic profiling revealed that treatment with HA + hUC-MSCs modulated proteins associated with complement activation and coagulation pathways. The combination of HA and hUC-MSCs provided synergistic benefits for cartilage regeneration in a sheep OA model during a short treatment period. These findings suggest that this approach represents a promising regenerative therapy for OA in large animals within veterinary medicine and has translational relevance for human regenerative medicine.
Co-Authors Aisyah Fidela Siregar Al Mukhlas Fikri Alfiah, Elma Alkaustariyah Lubis Almanda, Wisnu Rahmadan Arief Boediono Berry Juliandi Boenjamin Setiawan Budiariati, Vista Cece Sumantri Diah Nugrahani Pristihadi Dwi Budiono El Zamzami, Arfah Herawana Elmanaviean, Muhammad Gusdinar, Rizal Hardinsyah Harry Murti Harry Murti Ita Djuwita Katrin Roosita Kazuhiro Kikuchi KAZUHIRO KIKUCHI Kazuhiro Kikuchi Ketut Adnyane Mudite Kombo, Muhammad Piter Kusdiantoro Mohamad Lubis, Andri Maruli Tua M Agus Setiadi M. Haviz Makhroja, Lalu Faris Naufal Maria Ulfah Muhamad Rizal Martua Damanik Muhammad Gunawan Muhammad Gunawan Muhammad Rosyid Ridlo Ni Wayan Kurniani Karja Noer Muhammad Dliyaul Haq Nugraha, Arifin Budiman Nurhidayat Nurhidayat Nurwati, Yuni Nur’aisyah Amrah Safitri Pattikawa, Vapriel Andhika Peni Soeprapti Hardjosworo R. Iis Arifiantini Rahmaniyah, Wiwit Ridhani Rakhmawati, Handina Ratih Rinendyaputri Ratih Rinendyaputri Ratih Rinendyaputri Rini Widyastuti Rukmiasih Rukmiasih Safika S, Safika Situmeang, Adrian Sri Anna Marliyati Srihadi Agungpriyono Tatsuyuki Suzuki Tatsuyuki Suzuki, Tatsuyuki Triyaningrum, Triyaningrum Vetnizah Juniantito Wahono Esthi Prasetyaningtyas Wahono Esthi Prasetyaningtyas Wahono Esthi Prasetyaningtyas Wiwit Ridhani Rahmaniyah Yusuf Kurniawan Zulfikar, Muhammad Irsyad