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All Journal HAYATI Journal of Biosciences Jurnal Gizi dan Pangan Jurnal Ilmu Pertanian Indonesia ACTA VETERINARIA INDONESIANA Hemera Zoa Buletin Peternakan ILMU KELAUTAN: Indonesian Journal of Marine Sciences Jurnal Ilmu-Ilmu Peternakan (Indonesian Journal of Animal Science) Indonesian Journal of Biotechnology Jurnal Riset Veteriner Indonesia (Journal of The Indonesian Veterinary Research) Jurnal Kedokteran Hewan
Mokhamad Fahrudin
Departemen Anatomi Fisiologi Dan Farmakologi, Fakultas Kedokteran Hewan, Institut Pertanian Bogor, Jln Agathis, Dramaga, Bogor, Jawa Barat, Indonesia, 16680
Agriculture, Biological Sciences & Forestry Biochemistry, Genetics & Molecular Biology Earth & Planetary Sciences Health Professions Immunology & microbiology Materials Science & Nanotechnology Medicine & Pharmacology Veterinary Other

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Influence of Melatonin as an Antioxidant on the Preservation of Liquid Ram Semen in Tris-Fructose Egg Yolk Diluent Pattikawa, Vapriel Andhika; Karja, Ni Wayan Kurniani; Fahrudin, Mokhamad; Prasetyaningtyas, Wahono Esthi
Acta VETERINARIA Indonesiana Vol. 13 No. 1 (2025): Maret 2025
Publisher : IPB University

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.29244/avi.13.1.52-59

Abstract

Excessive free radicals can induce oxidative stress, leading to the inhibition of phosphorylation processes that supply energy for sperm capacitation and the acrosome reaction in spermatozoa during fertilization. This study investigated the impact of incorporating the antioxidant melatonin into the tris-fructose egg yolk diluent on the quality of ram liquid semen preserved at 4°C. Fresh semen with ≥70% motility was diluted in the tris-fructose egg yolk group (control), tris-fructose egg yolk with 0.5 mM melatonin (TFEY + M0.5), tris-fructose egg yolk with 1 mM melatonin (TFEY + M1), tris-fructose egg yolk with 2 mM melatonin (TFEY + M2). Semen was stored in the refrigerator at 4°C for 5 days (D0−D5). The motility, viability, abnormality, and plasma membrane integrity of the semen were evaluated every 24 hours. The results indicated no significant difference (P>0.05) between the control group and the liquid semen treatment at D0. Liquid semen quality was significantly improved with melatonin treatment compared to the control group. The results of this study indicate that TKTF + M0.5 had the highest motility and viability values ​​and can maintain abnormality values. The TKTF + M2 group had a higher plasma membrane integrity value than the other treatment groups.
Calcium Bioavailability and Serum Calcium Level in Pregnant Rats After Administration of Milk-Based Drinks Containing Lactic Acid Bacteria Nurwati, Yuni; Hardinsyah, Hardinsyah; Marliyati, Sri Anna; Fahrudin, Mokhamad
Jurnal Gizi dan Pangan Vol. 16 No. 3 (2021)
Publisher : The Food and Nutrition Society of Indonesia in collaboration with the Department of Community Nutrition, IPB University

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (291.107 KB) | DOI: 10.25182/jgp.2021.16.3.149-158

Abstract

This study aims to evaluate calcium bioavailability through serum calcium level in pregnant rats treated with two Milk-Based Drinks (MBD) containing Lactic Acid Bacteria (LAB), i.e Lactobacillus Casei Shirota Strain (LcS) drink, and Four Strains Bacteria (FS) (Lactobacillus rhamnosus, Lactobacillus paracasei, Lactobacillus delbrueckii subp. bulgaricus, Streptococcus thermophiles). It was a completely randomized experimental study using 24 Sprague Dawley rats. Rats were divided into one negative control group with a normal nutritional status (A0) and three undernourished groups. The undernourished groups were divided into one positive control group (A1), one group receiving MBD containing LcS group (A2), and another group receiving MBD containing FS (A3). All rats received high protein diets during pregnancy. The intervention was started in early pregnancy (D0) until 19 days of pregnancy (D19). Blood samples were collected at the D0 and D19. No significant differences in food intake were found among the rats in all groups. Administration of MBD containing LAB in A2 and A3 showed significant (p<0.05) increment of calcium bioavailability (30.79±6.88%; 20.44±9.04%). Both MBDs treatment containing LAB showed no significant difference in serum calcium bioavailability (p>0.05). The results suggest that MBDs containing LAB are useful in enhancing calcium bioavailability.
-TOCOPHEROL IMPROVED THE QUALITY OF BEAGLE DOG BREED CHILLED SEMEN STORAGE AT 4 C IN TRIS-SKIM MILK EXTENDER Makhroja, Lalu Faris Naufal; Juniantito, Vetnizah; Fahrudin, Mokhamad; Prasetyaningtyas, Wahono Esthi; Karja, Ni Wayan Kurniani
Jurnal Kedokteran Hewan Vol 19, No 3 (2025): September
Publisher : Universitas Syiah Kuala

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.21157/j.ked.hewan.v19i3.44561

Abstract

Improvement of canine reproduction has been developed by artificial insemination (AI) for effective breeding. -tocopherol widely recognized as an antioxidant that has been used to improve the quality of the spermatozoa. This study evaluated the effect of -tocopherol supplementation in dog chilled semen quality. Beagle dog breed semen samples were obtained from male Beagles using massage technique. The semen was then diluted with tris-skim milk extender supplemented with -tocopherol of various concentration, 0% as control group (T-0), 0.1% (T-0.1), 0.2% (T-0.2), and 0.3% (T-0.3), then were stored at 4 C and evaluated daily for four days, particularly for variables namely sperm motility, sperm viability, and plasma membrane integrity. Results showed that the quality of spermatozoa was higher when spermatozoa preserved in extender containing of 0.1% or 0.2% -tocopherol compared to those of control and 0.3% -tocopherol group (P0.05). The highest quality found in group with 0.2% -tocopherol. In conclusion, supplementation -tocopherol 0.2% in dog chilled semen extender is effective for improving and maintaining chilled dog sperm quality for 4 days of storage.
THE QUALITY OF CHILLED RAM SEMEN AFTER STORAGE IN EXTENDER WITH ADDITION OF MELATONIN AT 4 C Triyaningrum, Triyaningrum; Fahrudin, Mokhamad; Karja, Ni Wayan Kurniani; Prasetyaningtyas, Wahono Esthi
Jurnal Kedokteran Hewan Vol 18, No 1 (2024): March
Publisher : Universitas Syiah Kuala

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.21157/j.ked.hewan.v18i1.34649

Abstract

Semen was collected using an artificial vagina, and then examined macroscopically and microscopically. Semen with motility of 70% was diluted into different extenders: tris egg yolk-glucose (TEYG), tris egg yolk-glucose extender with 0.5 mM melatonin (TEYG + M0.5), tris egg yolk-glucose extender with 1 mM melatonin (TEYG + M1), tris egg yolk-glucose extender with 2 mM melatonin (TEYG + M2). All semen samples were stored at 4 C for 5 days (H0-H5) and examined every 24 hours. Semen was examined for spermatozoa motility, viability, abnormality, and membrane plasma integrity. The results showed that there were no significant changes of semen quality on H0 (P0.05). In general, the melatonin supplemented groups had a higher semen quality value than the control. Melatonin supplementation can protect and maintain the quality of chilled semen. In addition, the result showed that TEYG + M1 extender was the best extender treatment, which was indicated by higher values than other treatments.
PERKEMBANGAN EMBRIO SAPI SETELAH FERTILISASI MENGGUNAKAN METODE INTRACYTOPLASMIC SPERM INJECTION (ICSI) DAN AKTIVASI DENGAN STRONTIUM Gunawan, Muhammad; Fahrudin, Mokhamad; Boediono, Arief
Jurnal Kedokteran Hewan Vol 8, No 2 (2014): September
Publisher : Universitas Syiah Kuala

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.21157/j.ked.hewan.v8i2.2651

Abstract

Penelitian ini bertujuan mengetahui pengaruh kombinasi intracytoplasmic sperm injection (ICSI) dan aktivasi dengan strontium untuk mengetahui perkembangan pronukleus dan perkembangan embrio sampai tahap blastosis. Kombinasi ICSI dan strontium 20 mM meningkatkan perkembangan pronukleus 2-PN mencapai 43,59%. Hasil perkembangan embrio pada perlakuan kombinasi ICSI dan strontium 20 mM mencapai tingkat perkembangan 2-4 sel (50,5%), 8-16 sel (43,73%), dan blastosis (15,63%). Kesimpulan dari hasil penelitian ini adalah kombinasi ICSI dengan aktivasi strontium 20 mM mampu menghasilkan perkembangan in vitro embrio sapi yang lebih baik.
The safety evaluation of ethanol extract of torbangun leaves (Coleus amboinicus Lour.) on mouse fetal development Alfiah, Elma; Damanik, Muhammad Rizal Martua; Roosita, Katrin; Fahrudin, Mokhamad
Jurnal Gizi dan Pangan Vol. 13 No. 1 (2018)
Publisher : The Food and Nutrition Society of Indonesia in collaboration with the Department of Community Nutrition, IPB University

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (4631.317 KB) | DOI: 10.25182/jgp.2018.13.1.27-32

Abstract

The purpose of this study was to evaluate the safety of ethanol extract from torbangun leaves on mouse fetal development. This research used 24 female pregnant mouse. The leaves extract was administered orally at dose level of 0; 0.56; 1.68 and 3.36 g/kg body weight. The extract was given from the first day until the eighteenth day of pregnancy period. The ethanol extract of Torbangun leaves contains flavonoids, saponins, tannins, and steroids. The result showed that consumption of 3.36 g/kg torbangun leaves extract had led to significant differences in the decrease of maternal weight change, number of live fetuses, fetal weight, and fetal length. Consumption of Torbangun leaves extract during pregnancy should be avoided due to its potency to interfere the fetal development.  
Co-Authors Aisyah Fidela Siregar Al Mukhlas Fikri Alfiah, Elma Alkaustariyah Lubis Arief Boediono Berry Juliandi Boenjamin Setiawan Budiariati, Vista Cece Sumantri Diah Nugrahani Pristihadi Dwi Budiono Elmanaviean, Muhammad Gusdinar, Rizal Hardinsyah Harry Murti Ita Djuwita Katrin Roosita Kazuhiro Kikuchi Kazuhiro Kikuchi KAZUHIRO KIKUCHI Ketut Adnyane Mudite Kusdiantoro Mohamad M Agus Setiadi M. Haviz Makhroja, Lalu Faris Naufal Maria Ulfah Muhamad Rizal Martua Damanik Muhammad Gunawan Muhammad Gunawan Muhammad Rosyid Ridlo Ni Wayan Kurniani Karja Noer Muhammad Dliyaul Haq Nugraha, Arifin Budiman Nurwati, Yuni Nur’aisyah Amrah Safitri Pattikawa, Vapriel Andhika Peni Soeprapti Hardjosworo R. Iis Arifiantini Rahmaniyah, Wiwit Ridhani Ratih Rinendyaputri Ratih Rinendyaputri Ratih Rinendyaputri Rini Widyastuti Rukmiasih Rukmiasih Sri Anna Marliyati Srihadi Agungpriyono Tatsuyuki Suzuki Tatsuyuki Suzuki, Tatsuyuki Triyaningrum, Triyaningrum Vetnizah Juniantito Wahono Esthi Prasetyaningtyas Wahono Esthi Prasetyaningtyas Wahono Esthi Prasetyaningtyas Wiwit Ridhani Rahmaniyah Yusuf Kurniawan Zulfikar, Muhammad Irsyad