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All Journal Farmasains : Jurnal Farmasi dan Ilmu Kesehatan Journal of Tropical Life Science : International Journal of Theoretical, Experimental, and Applied Life Sciences Jurnal Kedokteran Brawijaya The Journal of Experimental Life Sciences (JELS) Biotropika Indonesian Journal of Biotechnology Indonesian Journal of Chemistry Biota Jurnal Riset Biologi dan Aplikasinya JSMARTech : Journal of Smart Bioprospecting and Technology Berkala Penelitian Hayati Tri Dharma Mandiri: Diseminasi dan Hilirisasi Riset Kepada Masyarakat (Jurnal Pengabdian Kepada Masyarakat) Makara Journal of Science
fatchiyah .
Departement Of Biology, Faculty Of Mathematic And Natural Science, Universitas Brawijaya, Malang
Agriculture, Biological Sciences & Forestry Biochemistry, Genetics & Molecular Biology Chemical Engineering, Chemistry & Bioengineering Chemistry Education Environmental Science Health Professions Immunology & microbiology Materials Science & Nanotechnology Medicine & Pharmacology Neuroscience Public Health Social Sciences

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Anti-Apoptotic Activity of Anthocyanins has Potential to inhibit Caspase-3 Signaling Sari, Dewi Ratih Tirto; Safitri, Anna; Cairns, James Robert Ketudat; Fatchiyah, Fatchiyah
Journal of Tropical Life Science Vol 10, No 1 (2020)
Publisher : Journal of Tropical Life Science

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (1484.263 KB) | DOI: 10.11594/jtls.10.01.03

Abstract

Caspase-3 is a biochemical marker for cell apoptosis. Several studies focused on exploring caspase inhibitor potential in natural compounds. Hence, in this study investigated the anthocyanins as anti-apoptotic potential activity through caspase-3 using molecular docking. Six types of anthocyanin were retrieved from PubChem database and caspase-3 protein was downloaded from Protein Data Bank. Anthocyanins and caspase-3 protein were docked using HEX 8.0 program and visualized using Discovery Studio 4.1 software. The interaction among cyanidin-3-O-glucoside, delphinidin-3-O-glucoside, pelargonidin-3-O-glucoside, peonidin-3-O-glucoside and petunidin-3-O-glucoside showed similar binding pattern on caspase-3 protein. All of them bind to BIR2 region and allosteric site of caspase-3, which are a crucial site for apoptosis regulation. Interestingly, malvidin-3-O-glucoside also interacted with caspase-3 in BIR1, BIR2 and BIR3 regions. In addition, anthocyanins-caspase-3 complex showed low energy and demonstrated several hydrogen bonds, hydrophobic interactions and van der Waals interactions, which indicated stable interaction. This study implies that all anthocyanins have potential as inhibitor of caspase-3 protein and might have potential as anti-apoptosis. Further in-vitro and in-vivo studies are need to confirm this experimental.
Level of mRNA Insulin Gene and Blood Glucose STZ-Induced Diabetic Rat are Improved by Glucomannan of Amorphophallus muelleri Blume from East Java Forest Indonesia Fatchiyah, Fatchiyah; Nurmasari, Damai Aulia; Masruro, Nuri; Rohmah, Rista Nikmatu; Triprisila, Lidwina Faraline; Mulyati, Mulyati; Yamada, Takahisa; Ohta, Takashi
Journal of Tropical Life Science Vol 9, No 2 (2019)
Publisher : Journal of Tropical Life Science

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (476.089 KB) | DOI: 10.11594/jtls.09.02.05

Abstract

Diabetes mellitus is one of metabolic disorder with high level of blood sugar glucose and reduces pancreatic-insulin levels that are altered by unhealthy food and lifestyle. To manage the blood glucose and insulin level, we need the diabetes dietary management related with insulin gene cascade. The purpose of this study was to investigate the influence glucomannan fiber to mRNA level of the insulin gene and genes cascade expression of target cells of type 1 diabetic rat. Three-month-old male (Rattus norvegicus strain Wistar) were fed with fiber of glucomannan of Amorphophallus muelleri Blume with dosage 0,06g/kg BW and 0.12g/kg BW every day during one month. RNA dot blotting with specific cDNA probe was performed mRNA level of insulin gene. The result of this study showed that glucomannan could reduce of body weight and blood glucose level significantly. The mRNA level of insulin gene of diabetic rats-treated 0.12g/kg BW glucomannan fiber dosage was the highest significance level among of all groups. In histopathological analysis, glucomannan was increasing significantly in IRS-1 level expression proliferate properly and consistently increasing of the PI3-K expression level in treated diabetic rats. This study indicates that the 0.12g/kg BW glucomannan was optimum dosage that effectively as role for alternative high-fiber therapy of type 1 diabetic through insulin receptor tyrosine kinase pathway. 
BZLF1 Expression of EBV is correlated with PARP1 Regulation on Nasopharyngeal Carcinoma Tissues fajri, wahyu nur laili; Rofi'i, Ahmad; Fatchiyah, Fatchiyah
Journal of Tropical Life Science Vol 3, No 1 (2013)
Publisher : Journal of Tropical Life Science

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Abstract

Nasopharyngeal carcinomas (NPC) is a cancer that arises in the epithelial tissue that covers the inside of the nasopharyngeal mucosa and nasopharynx. Infected Epstein Barr Virus (EBV) cell in a latent infection associated with the expression of nine latent proteins. Latent Membrane Protein 1 (LMP1) is one of latent proteins, and mayor EBV oncoprotein, with functions including virus growth, and to activate BamHI-Z Leftward Reading Frame 1 (BZLF1)-EBV, which can inhibit p53 to induce apoptotic resistance, metastasis, and immune modulation. The body will respond to the expansion of EBV infection with activation of Poly(ADP-ribose)Polymerase-1 (PARP1). The objective of study is to observe the expression of BZLF1 and determine PARP1 regulation in nasopharyngeal tissues. NPC-T2, NPC-T3 and polyp tissues slides are from Ulin Hospital, Banjarmasin. To characterize the necrotic cells such as pyknosis, karyorrhexsis, and karyolysis, histological slides were stained by HE that the necrotic cells measured by using a BX-53 microscope (Olympus) with CellSens Standard software. Tissues slides were stained by using immunofluorohistochemistry with EBV-BZLF1 antibody-Mouse anti-EBV monoclonal antibody against Goat anti-mouse IgG-FITC and anti-PARP1 antibody (MC-10) against Goat anti-mouse IgG labeled Rhodamin. The expression intensities were measured by Confocal Laser Scanning Microscope (Olympus). The percentage number of necrotic cells and BZLF1 and PARP1 expression intensity were analyzed using SPSS 16.0 by one-way ANOVA test with α = 0.05, beside that we use correlate and regression analyze. The research showed that the amount of karryorhexis higher than pyknosis and karyolysis in both tissues. BZLF1 expression 1.79 INT/sel (in polyp), 2.76 INT/sel (NPC Type 2) and 4.36 INT/sel (NPC Type 3), PARP1 expression 2.25 INT/sel (in polyp), 3.31 INT/sel (NPC Type 2), dan 5.93 INT/sel (NPC Type 3).The high of intensity of expression BZLF1 induced the increasing of PARP1 expression, but not correlated with percentage of necrotic cell. Interestingly, in all tissues (NPC-T1, NPC-T2 and polyp) is increased number of karyolysis cells properly, but not in pyknosis and karryorrhexis cells. The conclusion of this study indicated that the BZLF1 induce PARP1 to repair DNA damage against EBV infection.Keywords: BZLF1, CLSM, EBV, nasopharyngeal carcinoma, PARP1.
Predominant Bacterial Diversity in Rheumatoid Arthritis Rat After Treated with Caprine CSN1S2 Protein Suyanto, Eko; Fatchiyah, Fatchiyah
Journal of Tropical Life Science Vol 11, No 2 (2021)
Publisher : Journal of Tropical Life Science

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.11594/jtls.11.02.01

Abstract

Introduction: Rheumatoid arthritis (RA) is an autoimmune and systemic inflammatory disease influenced by microbial abnormalities (dysbiosis) in the intestinal that affect changes in metabolism and immune system disorders. This study aimed to investigate the predominant intestinal microbiota in complete Freund’s Adjuvant-induced rheumatoid arthritis rats after treated with caprine milk CSN1S2 protein through fecal analysis based on PCR-DGGE and to construct the phylogenetic tree of bacteria as the evolutionary relationship. Method: The experimental animals were divided into 6 groups with 2 types of rat model, namely control rat (C group, CM group, and CY group) and RA rat (RA group, RAM group, and RAY group). Predominant cultivable microbiota was obtained by direct culture and analyzed using PCR-DGGE with some specific primers. The DNA sequences were analyzed and aligned using bioinformatics software to construct the phylogenetic tree. Results: We found that Lactobacillus group significantly increased in the control rat model and the predominant intestinal bacteria in RA rats were Enterococcus group (Enterococcus faecium and Enterococcus faecalis). Conclusions: The caprine milk CSN1S2 protein influences the composition of microbiota in RA rats with the emergence of predominant bacteria that are considered species of the Bacillus group, closely related to Bacillus coagulans, which can be promoted the growth of B. coagulans to suppress pathogenic bacteria in the development of RA disease.
Binding Inhibition Between Igf1r and Igf1 by Catechin of Black Tea Firdausi, Lina; Indra, M Rasjad; ., fatchiyah
Journal of Tropical Life Science Vol 2, No 3 (2012)
Publisher : Journal of Tropical Life Science

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Abstract

The natural compound of black tea is used as an alternative of obesity therapies in the world; particularly, the catechin family in tea leaves which has bioactive compounds such as EC, EGC and EGCG. Their bioactivity contributes to inhibit the ligand of Insulin-Like Growth Factor I Receptor (Igf-1r) binding-region to Igf-1 protein. To elucidate the inhibiton of Igf-1 expression and proliferating of Rattus norvegicus strain wistar adipose cell using black tea solution. The research used Rattus norvegicus strain wistar. After a 90-day treatment, the adipose tissues were picked up from the viscera of each experimental animal, and then the adipose tissues were embedded by paraffin. The paraffin sections were determined through immunohistochemistry with anti-Igf-1 antiserum, and were also analyzed through hematoxylin-eosin. A protein sequence of Igf-1, Igf-1r, and 3D structure of EC, EGC and EGCG from Gene Bank sites were used during in silico analysis. The sequences were aligned by BLAST program to identify the conserve and variable domain of IGF-1 protein isoforms. The 3D structures of IGF-1 and IGF-1R were constructed using Phyre program. The ligand among the 3D structures of IGF-1, IGF-1R and catechin compounds were analyzed using Hex 5.1 docking program. The data showed that the Igf-1 expression of adipose cells was reduced at 0,03 g/ml BTS and 0,045 g/ml BTS treatments. The result of BLAST analysis showed that IGF-1 (a, b, c, and d) isoforms conserved a domain from amino acid no 22 until 134; and this region was a variable region. The EGCG bound L1 domain of IGF-1R with E-total -235.3 KJ/mol which was lower than EC (-208,4 KJ/mol) and EGC (-142 KJ/mol). The total energy of IGF-1 (a, b, c, but not d isoform) which interacted with EGCG was around -223.7 KJ/mol, EC is -205.6 KJ/mol and EGC was -191.7 KJ/mol. However, EC, EGC and EGCG was only able to prevent the interaction between the L1 of IGF-1R with IGF-1 protein, but not the opposite.Keywords: Adipose cell, black-tea, proliferation, catechin, IGF-1, IGF-1R, inhibition, docking
Identification of Fingerprints Pattern in Mixed Family of Chinese-Javanese Ethnic Nikmah, Istiftakhun; Fatchiyah, Fatchiyah
Journal of Tropical Life Science Vol 7, No 3 (2017)
Publisher : Journal of Tropical Life Science

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.11594/jtls.07.03.12

Abstract

The aim of this study is to identify fingerprints patterns from the mixed family of Chinese-Javanese ethnic. The fingerprint patterns divided into three main patterns, i.e. arch, loop and whorl. The respondents were determined based on inclusion criteria (subject of research qualified as a sample) and the exclusion criteria (subjects which could not be used as a sample) through interviews and questionnaires. First, respondent’s fingers cleaned with alcohol or washed using soapy water. The patterns of fingerprint were taken by pressing one by one using an inked stamp pad. Then, printed on white paper which has been given a code and serial number of fingers for each respondent. The last step was to identify the specific characteristic of fingerprint patterns from the respondents and analyzed it while determining about similarity and variability of inter-generations. The results indicated that the mixed family of Chinese-Javanese ethnic had whorl variant pattern (plain whorl and double loop whorl) on the thumbs as a specific marker of Javanese ethnic. Otherwise, radial loop on the index and tented arch pattern on both index and little finger are the unique marker of Chinese ethnic. Thus, both Javanese and Chinese ethnic have co-dominant fingerprint patterns for mixed family of Chinese-Javanese ethnic.
Binding Inhibition Between Igf1r and Igf1 by Catechin of Black Tea Lina Firdausi; M Rasjad Indra; fatchiyah .
Journal of Tropical Life Science Vol. 2 No. 3 (2012)
Publisher : Journal of Tropical Life Science

Show Abstract | Download Original | Original Source | Check in Google Scholar

Abstract

The natural compound of black tea is used as an alternative of obesity therapies in the world; particularly, the catechin family in tea leaves which has bioactive compounds such as EC, EGC and EGCG. Their bioactivity contributes to inhibit the ligand of Insulin-Like Growth Factor I Receptor (Igf-1r) binding-region to Igf-1 protein. To elucidate the inhibiton of Igf-1 expression and proliferating of Rattus norvegicus strain wistar adipose cell using black tea solution. The research used Rattus norvegicus strain wistar. After a 90-day treatment, the adipose tissues were picked up from the viscera of each experimental animal, and then the adipose tissues were embedded by paraffin. The paraffin sections were determined through immunohistochemistry with anti-Igf-1 antiserum, and were also analyzed through hematoxylin-eosin. A protein sequence of Igf-1, Igf-1r, and 3D structure of EC, EGC and EGCG from Gene Bank sites were used during in silico analysis. The sequences were aligned by BLAST program to identify the conserve and variable domain of IGF-1 protein isoforms. The 3D structures of IGF-1 and IGF-1R were constructed using Phyre program. The ligand among the 3D structures of IGF-1, IGF-1R and catechin compounds were analyzed using Hex 5.1 docking program. The data showed that the Igf-1 expression of adipose cells was reduced at 0,03 g/ml BTS and 0,045 g/ml BTS treatments. The result of BLAST analysis showed that IGF-1 (a, b, c, and d) isoforms conserved a domain from amino acid no 22 until 134; and this region was a variable region. The EGCG bound L1 domain of IGF-1R with E-total -235.3 KJ/mol which was lower than EC (-208,4 KJ/mol) and EGC (-142 KJ/mol). The total energy of IGF-1 (a, b, c, but not d isoform) which interacted with EGCG was around -223.7 KJ/mol, EC is -205.6 KJ/mol and EGC was -191.7 KJ/mol. However, EC, EGC and EGCG was only able to prevent the interaction between the L1 of IGF-1R with IGF-1 protein, but not the opposite.Keywords: Adipose cell, black-tea, proliferation, catechin, IGF-1, IGF-1R, inhibition, docking
Co-Authors Abdullah Abdullah Adhya Dava Aligarh Yahya Adzral Alamsyah Agustin, Diah Eka Ahmad Hafidul Ahkam Akbar Farid Hasibuan Alam, Fajar Mustika Alvionita, Cicin Vinolia Anandari, Risma Nila Andyni, Regina Shania Anna Roosdiana Antonius Christianto Aris Soewondo Aru W Sudoyo Arumsari, Pamuji Lestari Atamimi, Fachrur Rozi Aulanni'am, Aulanni'am Bare, Yohanes Budiarti, Sarah Fadilah Cairns, James Robert Ketudat Choirunil Chotimah Christianto, Antonius Criswahyudianti, Elsa Rahmania Dewi Ratih Tirto Sari Dian Siswanto Djoko Wahono S Eko Suyanto Elan Herlina Elan Herlina, Elan Ernanin Dyah Wijayanti Ezra, Achmad Fadilla, Khalisa Fahmi, Muhamad fajri, wahyu nur laili Farida Rachmawati, Farida Fatma Yona, Hafidza Fauzi Yusuf, Fauzi Firdausi, Lina Gotoh, Takayuki Handono Kalim Harun Al Rasyid Damanik, Harun Al Rasyid Hasibuan, Akbar Farid Hazna Noor Meidinna Hermanto, Feri Eko Hose, Victor Alvianoes Guterez Husnah, Yeni Avidhatul Ilmiyah, Silvi Zakiyatul Iva Himmatul Aliyah Iva Himmatul Aliyyah Kamila, Fairuz Sarah Karuniasari, Nadaa Khairunnisa Hidaya, Amira Kurnianingsih, Nia Lidwina Faraline Triprisila Lidwina Faraline Triprisila Lina Firdausi M Rasjad Indra M Rasjad Indra Maekawa, Tatsuya Maisuroh, Dalilatul Mandai, Kouhei Mantow, Jellyta Pricilla Mardhiyah, Rihadatul Aisy Masruro, Nuri Miggy Uri Karitas Minnah, Siti Khaizatul Miyajima, Katsuhiro Muhammad Darwin P Mulyati Mulyati Muwaffiq Faza, Ahmad Nafisah, Wirdatun Najma Zahira Nakamura, Sanae Narwasthu, Sekararum Nathania, Nina Regina Naufal, Achmad Hanif Nia Kurniawan Nia Kurniawan Nia Kurniawan Nikmah, Istiftakhun Nurdiana Nurdiana Nurdiana Nurdiana Nurmasari, Damai Aulia Ohta, Takashi Ohta, Takeshi Palis, Christine Natalia Pertiwi, Kadita Octavia Pramudya, Muhammad Alif Imam Pratama, Ardo Cahya Putri Ramadhani, Putri Putri, Nenis Try Melani Putri, Siti Aqila Kharisma Rahmadini, Agnia Fadillah Rahmat Grahadi Rasjad Indra Rasjad Indra Reyhanditya, Davy Rijalullah, Muhammad Asyraf Rista Nikmatu Rohmah Rista Nikmatu Rohmah Rista Nikmatu Rohmah Rista Nikmatu Rohmah, Rista Nikmatu Rivqi Rifa Bia Rizka Vamelia Sulistya Ningrum Rizky Nurdiansyah Robiatul Adawiyah Rofi'i, Ahmad Rosyada, Nabila Nur Rosyadah, Nuraini Safira, Dona Safitri, Anna Sari, Dewi Ratih Tirto Sasase, Tomohiko Shafala Safa, Muhammad Shinohara, Masami Shinozaki, Yuichi Siti Nur Aisyah Soraya Widyasari Soraya Widyasari, Soraya Sri Rahayu Lestari Syafruddin Ilyas Talitha Pangestu, Twistka Tapiory, Adelia Adrianne Titin Andri Wihastuti Turhadi Turhadi Ulfah, Mumtaz Nabila Umar, Ja’far Uno, Kinuko Wachid, Nisa Nabila Aufa Wahyuningsih, Nadia Widadni, Vidya Utami Widyananda, Muhammad Hermawan Yamada, Takahisa Yamaguchi, Ayane Zaidah, Laili Nur Zain, Dhiyaa Syahlaa Bianca Febrinnisa Zyana Fithri Nur Faizah