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Anticancer Activity of The Ethanol Fraction of Gnetum gnemon L. Seeds on HeLa Cell Lines Rahmawati, Devi; Ningrum, Dhecella Winy Cintya; Kenyori, Ivana Kinanti; Febriansah, Rifki; Octavia, Melany Ayu; Hermawansyah, Adi
Mutiara Medika: Jurnal Kedokteran dan Kesehatan Vol 23, No 2 (2023): July
Publisher : Universitas Muhammadiyah Yogyakarta

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.18196/mmjkk.v23i2.18421

Cervical cancer results from abnormal cell development in the cervix and is caused by the Human Papillomavirus (HPV). Cancer treatment with chemotherapy drugs has dangerous side effects because it can attack normal cells. Gnetum gnemon L. seeds are known to possess anticancer potential. Therefore, natural ingredients are necessary to develop new cancer drug strategies with minimal side effects. This study aims to determine the anticancer activity of the ethanol fraction of Gnetum gnemon L. Seeds (EFGS) against HeLa cells. The research method used in this study was an identification test to detect compounds from EFGS using the TLC method and the HeLa cell cytotoxic test using the MTT Assay method. The results showed that EFGS contained stilbenoid group compounds based on TLC-Densitometry, with an Rf value of 0.22 cm. The cytotoxic test results on HeLa cells indicated an IC50 value of 784 µg/mL, classifying it as having the potential as a moderate category anticancer agent. Therefore, this study indicates that EFGS can be utilized as a chemopreventive agent.

SAGO STARCH AND SODIUM ALGINATE AS NATURAL CROSSLINKING FOR CAPSULE ALTERNATIVES Harimurti, Sabtanti; Mulyanti, Amelia Nur'afni; Kusnindyasita, Anisa; Widada, Hari; Febriansah, Rifki; Suwanda, Totok; Muhtadi, Muhtadi
Jurnal Farmasi Sains dan Praktis Vol 9 No 3 (September-December 2023)
Publisher : Universitas Muhammadiyah Magelang

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.31603/pharmacy.v9i3.8856

Capsule shells are generally made from gelatin. The gelatin that is spread in the world is commonly derived from pigs. This animal is forbidden to be consumed by Muslims. This issue is due to the halal aspect. Sago starch and sodium alginate are halal materials that are capable of gelatinizing. This property made it able to be molded as a capsule. This research aims to determine the formula of sago and sodium alginate as a capsule and the evaluation. Three formulas were made with the ratio of sago and sodium alginate 100%: 0% (formula 1), 50%: 50% (formula 2), and 0%: 100% (formula 3). The capsule was manually molded using a food-grade stainless steel capsule mold. The size of the capsule, weight uniformity, swelling, and disintegration time were determined for a physical evaluation. SLD was used to determine the best formula, and the validation was done using One-sample T-test. The evaluations of the capsule shell were found ranged as follows: the size specifications were 21-22 mm, the body diameter was 6.1-7.2 mm, the cap diameter was 13.6-13.8 mm, weight uniformity was 0.10-0.26 gram, the swelling test was 433-1583%, and the disintegration time was 10.20-14.43 minutes. The best formula based on the SLD of the experiment was formula 3, which are two parameters that met the requirements, i.e., swelling and disintegration time. The sago and sodium alginate were crosslinked, and the capsule was made. However, the performance dislike of gelatin capsules. Continued research shall be done to find the optimum formula and its characteristics for alternative material on halal capsules.

Acute toxicity of the intranasal administration of Anredera cordifolia extract in Wistar Rats Widuri, Asti; Febriansah, Rifki
Pharmaciana Vol. 14 No. 2 (2024): Pharmaciana
Publisher : Universitas Ahmad Dahlan

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.12928/pharmaciana.v14i2.28249

Anredera cordifolia (AC) known as binahong plant in Indonesia is commonly used for traditional medicine since ancients. AC contains of secondary metabolites such as flavonoids that have anti-oxidative, anti-inflammatory, anti-mutagenic and anti-carcinogenic properties. Many flavonoids compound that has anti-inflammatory activity were potential for treatment of nasal inflammatory such as allergic rhinitis. This study aims to evaluate the preclinical safety of acute intranasal administration of AC extract in laboratory wistar rats. Acute toxicity using the intranasal route of administration of AC extract was evaluated on 30 female wistar rats, divided five rats for control, and each five of doses 5%, 10%, 25%, 50% and 75%. Observation during 14 days for the incidence of mortality and signs of toxicity. The AC extract intranasal administration doses at 5%, 10%, 25%, 50% and 75% did not show mortality or treatment related adverse events and did not showed significantly changes on blood profile. The AC extract was found safe until 75% for nasal administration in wistar rats.

Ursolic Acid and Polydatin in Melinjo Seeds Inhibit AKT1 and GAPDH Protein and HTB-179 Cells Migration Febriansah, Rifki; Julita, Irna
Majalah Obat Tradisional Vol 29, No 3 (2024)
Publisher : Faculty of Pharmacy, Universitas Gadjah Mada

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.22146/mot.88227

The incidence of lung cancer in Indonesia by 2020 has reached 34.783 cases. Melinjo contains ursolic acid and polydatin, which can suppress cell proliferation and induce cell apoptosis, respectively. This study aims to determine the Melinjo seed ethyl acetate fraction (MSEAF) ability to inhibit lung cancer proliferation and migration towards HTB-179 cells using in vitro and in silico methods. Melinjo seed powder was macerated using 70% ethanol and fractionated with ethyl acetate. The fraction obtained was then analyzed using HPLC to detect the active compounds. The compounds obtained were further analyzed using bioinformatics to determine the target proteins. The docking method was performed between ursolic acid and polydatin compounds with each target protein to determine the binding affinity. The in vitro test was done using the MTT cytotoxicity assay and scratch wound healing assay methods. The results showed that MSEAF contains ursolic acid and polydatin with retention times of 12,475 minutes and 16,564 minutes, respectively. Ursolic acid protein targets were TP53 and AKT1 with docking scores of -6,3 kcal/mol and -7,4 kcal/mol, while polydatin target proteins were GAPDH and VEGFA with docking scores of -8,8 kcal/mol and -5,5 kcal/mol. The results of the MTT assay showed an IC50 value of 35,539 g/mL, and MSEAF inhibited the migration of HTB-179 cells by slowing the migration rate. This study suggested that the MSEAF contained ursolic acid and polydatin, which exhibited the ability to prevent the growth and migration of HTB-179 lung cancer, supported by the prediction of their ability to bind to TP53 and AKT1 proteins.

Ethnomedicine Study on Medicinal Plants in Nanggulan District, Kulon Progo Regency Kintoko, Kintoko; Saputri, Ginanjar Zukhruf; Novitasari, Putri Rachma; Desmayanti, Astri; Witasari, Hardi Astuti; Ranti, Imaniar; Yulianto, Yulianto; Febriansah, Rifki; Widyaningrum, Rachmawati; Utami, Febri; Setiyawati, Fitri Indah
Journal of Food and Pharmaceutical Sciences Vol 13, No 1 (2025): J.Food.Pharm.Sci
Publisher : Integrated Research and Testing Laboratory (LPPT) Universitas Gadjah Mada

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.22146/jfps.14189

Despite the advancements in conventional medicine, medicinal plants continue to play an important role in treating different human ailments, particularly in developing nations. This is based on locals' knowledge of medicinal plants for treating various ailments. Ethnomedicine is a branch of research investigating society's local wisdom for maintaining its health. According to diverse field studies, 40 different varieties of plants have been discovered that the native inhabitants of Kulon Progo Regency think are medicinal. The purpose of this research was to identify therapeutic herbs used by the Kulon Progo population. Traditional healers and members of the Kulon Progo village were interviewed as part of this study. The ethnomedicine data was analyzed using Use Value (UV), Frequency of Citation (FC), and Plant Part Value (PPV). The analytical results suggest that turmeric (0.78), galangal (0.67), and ginger (0.67) are the most important plants to society. Rhizomes (38.10%) and leaves (34.52) are the most commonly employed plant parts for medicinal purposes. Boiling it (47.06%) produces herbal medication from the plant extract.

Ethnomedicine Study on Medicinal Plants in Nanggulan District, Kulon Progo Regency Kintoko, Kintoko; Saputri, Ginanjar Zukhruf; Novitasari, Putri Rachma; Desmayanti, Astri; Witasari, Hardi Astuti; Ranti, Imaniar; Yulianto, Yulianto; Febriansah, Rifki; Widyaningrum, Rachmawati; Utami, Febri; Setiyawati, Fitri Indah
Journal of Food and Pharmaceutical Sciences Vol 13, No 1 (2025): J.Food.Pharm.Sci
Publisher : Integrated Research and Testing Laboratory (LPPT) Universitas Gadjah Mada

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.22146/jfps.14189

Despite the advancements in conventional medicine, medicinal plants continue to play an important role in treating different human ailments, particularly in developing nations. This is based on locals' knowledge of medicinal plants for treating various ailments. Ethnomedicine is a branch of research investigating society's local wisdom for maintaining its health. According to diverse field studies, 40 different varieties of plants have been discovered that the native inhabitants of Kulon Progo Regency think are medicinal. The purpose of this research was to identify therapeutic herbs used by the Kulon Progo population. Traditional healers and members of the Kulon Progo village were interviewed as part of this study. The ethnomedicine data was analyzed using Use Value (UV), Frequency of Citation (FC), and Plant Part Value (PPV). The analytical results suggest that turmeric (0.78), galangal (0.67), and ginger (0.67) are the most important plants to society. Rhizomes (38.10%) and leaves (34.52) are the most commonly employed plant parts for medicinal purposes. Boiling it (47.06%) produces herbal medication from the plant extract.

Uji molecular docking dan bioinformatika terhadap meniran (Phyllanthus niruri L.) sebagai antivirus SARS-CoV-2 dan antikanker serviks Nuha Haifa ARIFIN; Rifki FEBRIANSAH
Menara Perkebunan Vol. 90 No. 1 (2022): 90 (1), 2022
Publisher : INDONESIAN OIL PALM RESEARCH INSTITUTE

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.22302/iribb.jur.mp.v90i1.477

Abstract The SARS-CoV-2 virus is a virus that emerged in late 2019 and has yet to find a cure. On the other hand, the incidence of cervical cancer in women continues to increase along with the emergence of cases of COVID-19 caused by SARS-CoV-2. Based on WHO data in 2020 stated that there were 107 per 72,314 cancer patients infected with SARS-CoV-2. Meniran (Phyllanthus niruri L.) is a herbaceous plant in Indonesia that has secondary metabolites derived from the tannin group, such as corilagin. This compound has the potential to be developed as an antiviral and anticancer agent. Thus, the purpose of this study was to determine the potential of corilagin in meniran herbs to act as an antiviral SARS-CoV-2 and cervical anticancer compared to the drug compounds molnupiravir and paclitaxel through the STITCH & STRING bioinformatics in silico test and molecular docking method. The results of the bioinformatics test of corilagin against the SARS-CoV-2 virus showed predictions of high protein binding to AGTR2 and ENPEP with a docking score of -10.9 and -9.9 kcal/mol, respectively. Meanwhile, cervical cancer cells showed the highest predicted protein binding to IL-10 and MAPK3 with a docking score of -10.5 and -10.8 kcal/mol. The docking score of molnupiravir against the COVID-19 virus protein, AGTR2, and ENPEP were -7.4 and -7.2 kcal/mol, respectively. The docking scores of paclitaxel for IL10 and MAPK3 were -8.2 and -8.9 kcal/mol, respectively. These values indicate that the activity of corilagin with proteins AGTR2, ENPEP, IL10, and MAPK3 has stronger affinity energy than the comparison drugs molnupiravir and paclitaxel. Thus, the corilagin compound from the tannin group in meniran (Phyllanthus niruri L.) has the potential to be developed and formulated as a treatment and prevention of SARS-CoV-2 antiviral and cervical anticancer.[Keywords: corilagin, cervical cancer, Phyllanthus niruri L., SARS-CoV-2] Abstrak Virus SARS-CoV-2 merupakan virus yang muncul di akhir tahun 2019 dan belum ditemukan pengobatannya. Di sisi lain, terjadinya kasus kanker serviks pada wanita masih terus meningkat beriringan dengan munculnya kasus COVID-19 yang disebabkan oleh SARS-CoV-2. Berdasarkan data studi WHO tahun 2020 menyatakan bahwa terdapat 107 per 72.314 pasien kanker yang terinfeksi SARS-CoV-2. Meniran (Phyllanthus niruri L.) merupakan tanaman herba di Indonesia yang memiliki senyawa metabolit sekunder yang berasal dari golongan tannin seperti corilagin. Senyawa ini memiliki potensi untuk dikembangkan sebagai agen antivirus dan antikanker. Penelitian ini bertujuan untuk mengetahui potensi corilagin yang terkandung pada herba meniran sebagai antivirus SARS-CoV-2 dan antikanker serviks yang dibandingkan dengan senyawa obat molnupiravir dan paclitaxel melalui uji in silico bioinformatika STITCH & STRING serta metode molecular docking. Hasil uji bioinformatika corilagin terhadap virus SARS-CoV-2 menunjukkan prediksi pengikatan protein yang tinggi pada AGTR2 dan ENPEP dengan docking score -10,9 dan -9,9 kcal/mol. Sedangkan pada sel kanker serviks menunjukkan prediksi pengikatan protein tertinggi pada IL-10 dan MAPK3 dengan docking score -10,5 dan -10,8 kcal/mol. Docking score molnupiravir terhadap protein virus Covid-19, AGTR2 dan ENPEP adalah -7,4 dan -7,2 kcal/mol. Docking score paclitaxel terhadap IL10 dan MAPK3 adalah -8,2 dan -8,9 kcal/mol. Nilai tersebut menunjukkan aktivitas corilagin dengan protein AGTR2, ENPEP, IL10, dan MAPK3 memiliki energi afinitas yang lebih kuat dibandingkan senyawa obat pembanding molnupiravir dan paclitaxel. Sehingga, senyawa corilagin dari golongan tannin pada meniran (Phyllanthus niruri L.) memiliki potensi untuk dikembangkan dan diformulasikan sebagai pengobatan serta pencegahan antivirus SARS-CoV-2 dan antikanker serviks.[Kata kunci: corilagin, kanker serviks, Phyllanthus niruri L., SARS-CoV-2]

Acute toxicity of the intranasal administration of Anredera cordifolia extract in Wistar Rats Widuri, Asti; Febriansah, Rifki
Pharmaciana Vol. 14 No. 2 (2024): Pharmaciana
Publisher : Universitas Ahmad Dahlan

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.12928/pharmaciana.v14i2.28249

Anredera cordifolia (AC) known as binahong plant in Indonesia is commonly used for traditional medicine since ancients. AC contains of secondary metabolites such as flavonoids that have anti-oxidative, anti-inflammatory, anti-mutagenic and anti-carcinogenic properties. Many flavonoids compound that has anti-inflammatory activity were potential for treatment of nasal inflammatory such as allergic rhinitis. This study aims to evaluate the preclinical safety of acute intranasal administration of AC extract in laboratory wistar rats. Acute toxicity using the intranasal route of administration of AC extract was evaluated on 30 female wistar rats, divided five rats for control, and each five of doses 5%, 10%, 25%, 50% and 75%. Observation during 14 days for the incidence of mortality and signs of toxicity. The AC extract intranasal administration doses at 5%, 10%, 25%, 50% and 75% did not show mortality or treatment related adverse events and did not showed significantly changes on blood profile. The AC extract was found safe until 75% for nasal administration in wistar rats.

Synthesis and Characterization of Ethanolic Extract of Red Betel Leaf as an Antiseptic Gel Harimurti, Sabtanti; Hidayaturahmah, Rizky; Arsito, Puguh Novi; Febriansah, Rifki; Widada, Hari
Jurnal Farmasi Sains dan Komunitas (Journal of Pharmaceutical Sciences and Community) Vol 21, No 1 (2024)
Publisher : Sanata Dharma University

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.24071/jpsc.006555

Hand hygiene is one way to maintain health. There are several ways to clean hands, namely by washing hands and applying a hand sanitizer. The use of hand sanitizer is increasing due to its practical nature. Utilizing natural materials for preparing hand sanitizer widely available around us will be beneficial, one of which is red betel leaf which some people of Indonesia empirically use for antiseptic. This study aims to develop antiseptic gel preparations with ethanolic extracts from red betel leaves. The extraction was done using maceration with 70% ethanol solvent. The formula for choosing a carbomer as a gelling agent with the red betel leaf extract concentration was 0%, 2.5%, 5%, 10%, and 15%. The assessments of the gel were physical and antiseptic evaluations. The physical evaluations included an organoleptic, pH, viscosity, adhesion, and dispersion tests. The antiseptic activity was determined by a replica method. The physical evaluation results of red betel gel revealed that the higher the red betel leaf extract levels are, the darker the green color will be, and the lower the pH and viscosity will be. Furthermore, the antiseptic activity showed that red betel extract gel effectively reduced the number of bacterial colonies.

Bioinformatic Test and Pharmacokinetic Profile Prediction of Gnetin-C Compound in Melinjo (Gnetum gnemon L.) Seeds Toward Colorectal Cancer Kenyori, Ivana Kinanti; Febriansah, Rifki
Indonesian Journal of Cancer Chemoprevention Vol 15, No 1 (2024)
Publisher : Indonesian Society for Cancer Chemoprevention

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.14499/indonesianjcanchemoprev15iss1pp18-25

Cancer is one of the unresolved health problems in the world, including Indonesia. One of the most common types of cancer is colorectal cancer. Melinjo (Gnetum gnemon L.) is one of Indonesian local commodities which has many benefits including its potential to be developed as an anticancer agent. Through bioinformatics and molecular docking technology, the aim of this study was to investigate the functions of gnetin-C from melinjo against colorectal cancer. PkCSM database were used to search the ADMET (absorption, distribution, metabolism, elimination and toxicity) properties and stitch-string database were used to identify common genes related to colon cancer. During identification of colon cancer related genes, STAT3 protein showed the highest degree score. Furthermore, molecular docking was carried out to find out the interaction between the STAT3 protein and gnetin c compound found in melinjo seeds. From the docking stage, the pose with the best affinity energy was obtained with a docking score of 1,966 kcal/mol. this shows that the compound gnetin c has the potential to be used as a new anticancer agent from natural ingredients.Keywords: Melinjo, Gnetum gnemon, colon cancer, bioinformatics, molecular docking.

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