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Hepatoprotective Effect of Ethanolic Extract of Pegagan Leaves (Centella asiatica L. Urban) on Histological Damage of Hepatocyte on Mice Model induced by Parasetamol Putri, Naila Shofwati; ., Suyatmi; Febrinasari, Ratih Puspita
Nexus Biomedika Vol 4, No 1 (2015): Nexus Biomedika
Publisher : Nexus Biomedika

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Abstract

Background: Pegagan leaves (Centella asiatica L. Urban) contain antioxidative compounds that can protect hepatocytes from free radicals. The aim of this research was to know the hepatoprotective effect of ethanolic extract of pegagan leaves (Centella asiatica L. Urban) on histological damage of hepatocyte on mice model induced by paracetamol. Methods: This study was an experimental laboratory with the post test only control group design. Samples were 30 male Swiss webster mice, with 2-3 months old and ± 20 gr body weight divided into 5 groups equally. KN and KI were given distilled water for 14 days. In addition, KI was also given paracetamol in dose 5.07 mg/20 gbw on the 12th, 13th, 14th day. KP1, KP2, and KP3 were given ethanolic extract of pegagan leaves in dose 5 mg, 10 mg, and 20 mg per 20 gbw respectively for 14 days and paracetamol in dose 5.07 mg per 20 gbw on the 12th, 13th, 14th day. On the 15th day, mice were sacrificed and their livers were taken for preparation with paraffin block method and stained with HE. The features of histological damage of hepatocyte were assessed by counting the numbers of nucleus damage of hepotocyte (pyknosis, karyorrhexis, karyolysis) of 100 cells in the sentrolobular zone. The data were analyzed by One-way ANOVA test and Post Hoc LSD test. Results: The means of histological damage of hepatocyte were 16.08 ± 5.160; 66.00 ± 3.275; 44.58 ± 5.583; 31.67 ± 2.188; 26.17 ± 3.810 for KN, KI, KP1, KP2, KP3 respectively. The result of One-Way ANOVA test was *p = 0.000. The result of Post Hoc LSD test showed a  significant difference between five groups, *p = 0.000 for KN-KI, KN-KP1, KN-KP2, KN-KP3, KI-KP1, KI-KP2, KI-KP3, KP1-KP2, KP1-KP3 and *p = 0.002 for KP2-KP3. Conclusion: The ethanolic extract of pegagan leaves can prevent histological damage of hepatocyte on mice model induced by paracetamol. Keywords: ethanolic extract of pegagan leaves, paracetamol, histological damage of hepatocyte  
The Protection Effect of Centella asiatica L.Urban extract on the Kidney Histological Structure of Mice (Mus musculus ) induced by toxic doses of paracetamol Sakti, Berlian Permata; ., Muthmainah; Febrinasari, Ratih Puspita
Nexus Biomedika Vol 4, No 1 (2015): Nexus Biomedika
Publisher : Nexus Biomedika

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Abstract

Background: Centella asiatica L.Urban contains antioxidant compounds such as triterpenoid, essential oil and flavonoid. The compounds have significant activities to reduce oxidative stress that can make damage of kidney cells. This study aimed to investigate protection effect of Centella asiatica L.Urban extract on the kidney histological structure of mice (Mus musculus ) induced by toxic doses of paracetamol. Method: This study was an experimental laboratory research. Samples were 30 mice, Swiss webster type, 2-3 weeks old and ± 20 grams of weight. Samples were divided into 5 groups, each group consisted of 6 mice (Mus musculus). The control group (KK) was given no treatment, just regular feed. Induction group (KI) was induced by paracetamol but did not get any treatment, just got distilled water. KP1, KP2, and KP3 groups induced by paracetamol got treatment with Centella asiatica L.Urban extract at a dose of 5 mg, 10 mg and 20 mg per 20 gram of body weight. Kidney histological damage were assessed by the sum of the cell nucleus pyknosis, karyorrhexis, and karyolysis. Data were analyzed using one-way ANOVA and post hoc multiple comparison test. Result: The ANOVA test for all groups showed that p value was 0.000 (p < 0.05). The post hoc multiple comparisons test showed the significant differences between group KK-KI, KK-KP1, KK-KP2, KK-KP3, KI-KP1, KI-KP2, KI-KP3, KP1-KP2, KP1-KP3, and KP2-KP3. Conclusion: Centella asiatica L.Urban extract has a protection effect to kidney histological structure of mice (Mus musculus) induced by paracetamol. Keyword : Centella asiatica L.Urban, paracetamol, kidney damage.
Screening The Effectiveness of Meniran’s Leaf Extract (Phyllanthus niruri L.), Pepaya (Carica papaya L.), and Kunyit (Curcuma longa L.) as Antidengue In Vitro Ummiyati, Hutami Sri; Febrinasari, Ratih Puspita; Saptawati, Leli
Nexus Biomedika Vol 5, No 1 (2016): Nexus Biomedika
Publisher : Nexus Biomedika

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Introduction: Dengue Haemorrhagic Fever (DHF) is an infectious disease that is caused by dengue virus. There were 71.668 DHF cases with 641 mortalities in Indonesia which was recorded from early January to mid-December 2014. Recently, DHF doesn’t have a specific therapy. The aim of this study was screening about natural extracts in Indonesia as antidengue. Methods: This study was a laboratory experimental research with dengue virus serotype 2 strain New guinea C (DENV2 NGC) as a subject. This virus was infected into Huh-7 cell line. The study was conducted in 2 parts. First, dengue virus solution was mixed with natural extract and then this solution was infected to Huh-7 cell monolayer to obtain percent infectivity by FFU assay. Second,the natural extract was exposed to Huh-7 cell monolayer to obtain percent viability by MTT assay. The effective extract was selected if the extract had average percent infectivity £ 20% and average percent viability > 50%. Results: The percentage of viability of meniran’s leaf extract (Phyllanthus niruri L.), papaya (Carica papaya L.), and turmeric (Curcuma longa L.) were 96.2%, 97.5%, and 88.7% respectively. While their average percentage of infectivity were 63.9%, 10.5%, and 53.6%. Conclusion: The papaya’s leaf extract (Carica papaya L.) was effective as antidengue in vitro. Keywords: DENV2 NGC,dengue,  meniran, papaya’s leaf, turmeric 
Screening Effectiveness of The Leaf Extract of Psidium guajava, Euphorbia hirta, and Piper betle L. as Antidengue FAZA, AGYA GHILMAN; ., Hudiyono; Febrinasari, Ratih Puspita
Nexus Biomedika Vol 5, No 1 (2016): Nexus Biomedika
Publisher : Nexus Biomedika

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Introduction: Dengue Hemorrhagic Fever (DHF) is an infectious disease caused by dengue virus which commonly found in the tropic and sub-tropic region. Nowadays, the main treatment of DHF is supporting therapy by giving a replacement fluid. However, the number of cases and deaths due to dengue infection in Indonesia still high enough. Another solution to fix this problem is using Indonesian’s native plants which potentially has antiviral effect for dengue virus, such as guava leaf (Psidium guajava), patikan kerbau leaf (Euphorbia hirta), and betel leaf (Piper betle L.). The aim of this study is to determine the effectiveness of the leaf extract of guava (Psidium guajava), patikan kerbau (Euphorbia hirta), and betel (Piper betle L.) as antidengue in vitro. Methods: This study was laboratory experimental research. The subject of this research was dengue virus serotype 2 strain New Guinea C (DENV2 NGC) which was infected into Huh-7 cell line. The study was held in two places, they were Virology and Biology Molecular Laboratory, Department of Microbiology, Faculty of Medicine, University of Indonesia and Pharmaceutical Laboratory, Faculty of Mathematic and Science, Sebelas Maret University. The study was conducted in 2 parts. First, dengue virus solution was mixed with herbal extract in well which its bottom contained Huh-7 cell monolayer to obtain percent infectivity by FFU assay. Second, Huh-7 cell monolayer was exposed to the herbal extract solution to obtain percent viability by MTT assay. The effectiveness of herbal extract was based on two criterias, there were average percent infectivity £ 20% and average percent viability > 50%. Results: The leaf extract of Psidium guajava, Euphorbia hirta, and Piper betle L. have average percent infectivity respectively: 7.4%, 65.3%, and 68.4%. While the average percent viability of them were: 88.7%, 95.3%, 96.2%. Conclusion: The leaf extract of Psidium guajava is effective as antidengue in vitro. Keywords: Psidium guajava, Euphorbia hirta, Piper betle L., dengue virus serotype 2 strain New guinea C (DENV2 NGC), Huh-7 cell
The Influence of Broccoli Extract (Brassica oleracea var. Italica) to Liver Cells Damage in Mice (Mus musculus) Induced by Paracetamol Wulandari, Shinta Retno; Suparyanti, Endang Listyaningsih; Febrinasari, Ratih Puspita
Nexus Biomedika Vol 5, No 2 (2016): Nexus Biomedika
Publisher : Nexus Biomedika

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Introduction : Broccoli is known to contain antioxidants such as vitaminC, flavonoids and sulforaphane. Antioxidants can protect liver from damage caused by free radicals. This study aims to prove that broccoli extract protect liver cell from damage in mice induced by paracetamol in toxic dose and an increased doses of broccoli extract enhance the effects of protection to liver cell in mice. Methods : This study was an experimental laboratory with post test only controlled group design. Samples (30 mice) were taken by incidental sampling. Samples divided randomly into 5 groups, each group consisted of 6 mice. K1 was given distilled water. K2 was given paracetamol in toxic dose. KP1, KP2 and KP3 were given broccoli extract dose I, II and dose III respectively for 14 days and also were given paracetamol in toxic dose on day 12, 13 and 14. On day 15, the livers of mice were taken for making preparations with HE staining. The liver cell damages were observed by counting the nuclei of cells undergoing pyknosis, karyorrhexis and karyolysis. Data were analyzed by Oneway ANOVA and Post Hoc LSD analysis test (α = 0.05). Result : K2 had the most damaged cells (157.17 ± 7.834 cells) and the least damaged cells were at K1 (1.50 ± 1.049 cells). Oneway ANOVA test result was p = 0.00 (p < 0.05). Result of Post Hoc LSD analysis test showed a significant difference between groups on all pairs with p = 0.00 (p < 0.05). Conclusions : Broccoli extract protect liver cells from damage in mice induced by paracetamol. Increased doses of broccoli extract were proven to increase the protection effect on liver cells. Keywords: broccoli extract, paracetamol, liver cell damage 
The Effect of Soybean Powder (Glycine max) to the Histological Structure of Mice’s (Mus musculus) Liver Cells Induced by Used Cooking Oil Anggraeni, Selvia; Suparyanti, Endang Listyaningsih; Febrinasari, Ratih Puspita
Nexus Kedokteran Translasional Vol 3, No 1 (2014): Nexus Kedokteran Translasional
Publisher : Fakultas Kedokteran Universitas Sebelas Maret Surakarta

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Abstract

Background: Soybean contain antioxidants which have hepatoprotective effect; one of them is isoflavone. Soybean powder has the highest isoflavone among soybean products. This research aims to prove that the use of soybean powder can prevent damage of the histological structure of the mice’s liver cells induced by used cooking oil. Methods: This study was an experimental laboratory with a post-test only control group design. Twenty eight Swiss Webster male mice were divided into 4 groups. Negative control group (KN) was given distilled water, the positive control (KP) was given 0.06 ml/ 20 g BW used cooking oil, dose 1 (KD1) was given soybean powder 5.6 mg/ 20 g BW and used cooking oil, and dose 2 (KD2) was given soybean powder 11.2 mg/ 20 g BW and used cooking oil. Soybean powder was given for 14 days, while the used cooking oil was given orally on 8th-14th day. On the 15th day, mice were sacrificed, taken their liver, and made ​​preparations with HE staining. Liver cell damage was observed by counting the number of cells with pyknosis, karyorheksis, and karyolysis of 100 cells in liver centrolobular zone. Data were analyzed by statistical tests One-way ANOVA (Analysis of Variant) (α = 0.05). Results: The highest number of liver cells damage is on KP, followed by KD1, KD2, and at least on KN. Results of One-way ANOVA test showed significant differences among the four groups with p = 0.000 (p < 0.05). Results of Post Hoc Test of Multiple Comparasions using LSD test also showed significant differences between KN-KP, KN-KD1, KD2-KN, KP-KD1, KD2-KP, KD1-KD2 with respectively p = 0.000 (p < 0, 05). Conclusion: Soybean powder can prevent damage of the histological structure of the mice’s liver cells induced by used cooking oil. Keywords: soybean powder, used cooking oil, liver histological structure 
Effect of EthanolicExtract of Centella asiatica againts Adrenal Gland CortexCell Lipid Droplet Feature Induced by Chronic Immobilization Stress in Rats Zahran, Mochammad Fairuz; Wasita, Brian; Wiyono, Nanang; Febrinasari, Ratih Puspita
Nexus Kedokteran Translasional Vol 4, No 1 (2015): Nexus Kedokteran Translasional
Publisher : Fakultas Kedokteran Universitas Sebelas Maret Surakarta

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Background: Chronic stress can increase the lipid droplet in cortex cell and adrenal gland damage. Pegagan extract contains such as asiaticoside, flavonoids and triterpenoid are known have antioxidant effect. The objective of the present study was to investigate the beneficial effects of Ethanolic Extract of Centella asiatica (EEC) on adrenal gland cortexcell lipid droplet feature induced by immobilization stress in male rats. Method: This study was a laboratory experimental study with randomized post test only control group design. Paraffin blocks from thirty five male rats previously subjected with chronic immobilization stress and then divided into 6 groups: control group given distilled water 1 mg/kg, stress + PGA, stress + 150 mg/kg EEC, stress + 300 mg/kg EEC, stress + 600 mg/kg EEC and stress + fluoxetine 10 mg. The treatments were performed for 21 consecutive days. Lipid droplet was evaluated using Scoring Lipid Content from Hematoxylin Eosin staining. Statistic analysis was performed using Kruskall-Wallis test continued by Post Hoc Mann-Whitney Test with Bonferroni correction. Result: Results of Kruskall Wallis test showed significant differences in six groups with p = 0.000. Mann Whitney test with Bonferroni correction showed a significant difference between control group with stress + PGA and stress + 600 mg/kg EEC. Fluexetine as positive control show the most effective dose in this study (p = 0,000). There were no significant difference between fluoxetine as control positive and control group (p = 0,112). Conclusion: Stress + 600 mg/kg EEC has beneficial effect on preventing adrenal gland cortexcell lipid droplet feature induced by chronic immobilization stress in rat. Keywords : Centella asiatica, chronic immobilization stress, adrenal glands lipid droplet feature 
The Affecting Factors of Compliance Diabetes Mellitus Type 2 Treatment in Pandemic Era Febrinasari, Ratih Puspita; Solikah, Tri Agusti; Nasirochmi, Dyonisa; Dilma’aarij, Dilma’aarij
KEMAS: Jurnal Kesehatan Masyarakat Vol 17, No 4 (2022)
Publisher : Department of Public Health, Faculty of Sport Science, Universitas Negeri Semarang

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.15294/kemas.v17i4.29885

Abstract

Diabetes mellitus (DM) is one of the non-communicable diseases that cause serious health problems. This chronic disease requires lifelong treatment to prevent complications and death. In the pandemic era, DM became comorbid that contributed to the death rate in Covid-19 cases, so that patients with type 2 DM become important to comply with the treatment underway. Aim: This study aimed to evaluate the treatment compliance in patients with DM type 2 at the Purwodiningratan primary health care, Surakarta. Method: This study was conducted in 2020 used an analytical method with crosssectional design. Populations were all of the patients who experience DM type 2, sampling techniques used total sampling with 108 respondents. Univariate, bivariate analysis, and logistic regression were used in this study. Compliance levels measured used the MMAS-8 questionnaire. Result:  The results of this study were obtained from several independent variables that related to compliance with DM type 2 treatment. The descriptive analysis result of patient compliance showed that 70.4% of patients were obedient. The significant variable was the side effects on DM drugs with (OR = -0.44; 95% CI = -0.74 - 0.15; p = 0.003). Conclusion: Most of the patients were adherent to the treatment and the most influencing factor was the side effect of the drug.
The Relationship between Knowledge about Antibiotics with Usage Frequency of Non-prescription Antibiotics on Non-Medical Student of Sebelas Maret University Nadya Kemala Amira; Arsita Eka Prasetyawati; Ratih Puspita Febrinasari
Nexus Kedokteran Komunitas Vol 5, No 1 (2016): Nexus Kedokteran Komunitas
Publisher : Fakultas Kedokteran Universitas Sebelas Maret Surakarta

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Background: Antibiotics are the drugs most widely used and abused in the world. The most frequent form of antibiotic misuse is self-medication by various groups of society including students. Lacking of knowledge of the students, especially non-medical students, about antibiotics plays an important role in the discovery of many cases of antibiotics misuse. This study aimed to investigate the relationship between knowledge about antibiotics with usage frequency of non-prescription antibiotics on non-medical student of Sebelas Maret University. Method: This study was an observational analytic study with cross sectional approach. Sample collected from 186 non-medical students from 5 faculties in Sebelas Maret University which were randomly drawn by cluster random sampling technique. Each respondent was given a questionnaire regarding knowledge about antibiotics and the usage frequency of non-prescription antibiotics that has been tested for validity and reliability by researcher. The research data were tested with the nonparametric Spearman correlation test (? = 0.05). Result: Spearman correlation test results a significant correlation between knowledge about antibiotics with usage frequency of non-prescription antibiotics on non-medical students of Sebelas Maret University with p < 0.05 (p = 0.000). The test results also showed a strong correlation between the two variables with a correlation coefficient (r) -0.681, with increased knowledge about antibiotics will decrease the usage frequency of non-prescription antibiotics on non-medical students of Sebelas Maret University. Conclusion: There was a strong relationship between knowledge about antibiotics with the usage frequency of non-prescription antibiotics on non-medical students of Sebelas Maret University. Better knowledge of antibiotics, would reduce the usage frequency of non-prescription antibiotics. Keywords: antibiotics, knowledge about antibiotics, the usage frequency of non-prescription antibiotics
Pengaruh Pemberian Ekstrak Kulit Manggis (Garcinia Mangostana L.) Dalam Mencegah Peningkatan Kadar Proteinuria Tikus Putih (Rattus Norvegicus) Yang Diinduksi Gentamisin Asih Anggraini; Ratih Puspita Febrinasari; Nur Hafidha Hikmayani
Nexus Biomedika Vol 3, No 1 (2014): Nexus Biomedika
Publisher : Nexus Biomedika

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Abstract

Background : The increasing use of nephrotoxic drugs has increased the incidence of renal diseases. Research on herbs with potential nephroprotective effects is needed to offer adjuvant therapy for renal diseases. Mangosteen peel contains gamma-mangostin and antioxidant which can prevent renal damages. Methods : This research was a laboratory experiment with pre and post-test control group design. Subjects were 30 white, male, Wistar-strain rats (Rattus norvegicus), aged 2-3 months and weighed 150-230 grams. Subjects were randomly divided into 5 groups. Negative control group was treated nothing, while normal control group was treated 0.5% NaCMC. Treatment group 1, 2 and 3 were treated with mangosteen peel extract at dose 62.5 mg/kg-BW, 125 mg/kg-BW and 250 mg/kg-BW, respectively. The treatment was done for 14 days. In the last 7 days, the negative control group and the treatment groups 1, 2, and 3 were induced by gentamicin 100 mg/kg-BW. Urine samples of the subjects were collected before and after treatment to measure proteinuria levels. Data were analyzed by Kruskal-Wallis test and Mann-Whitney tests (? = 0.05) using SPSS 16.0 for Windows. Results : Result of Kruskal-Wallis test showed that proteinuria levels differed significantly across five groups (p = 0.008). The Mann-Whitney tests showed statistically significant differences between the negative control group and the other 4 groups (p < 0.05). No significant difference was found between the normal control group and the treatment groups 1, 2, and 3 (p > 0.05). Conclusion : Mangosteen (Garcinia mangostana L.) peel extract could prevent the increase of proteinuria levels in white rats (Rattus norvegicus) induced gentamicin. Keywords : Mangosteen Peel Extract, Proteinuria Level, Gentamicin
Co-Authors . Suyatmi . Suyatmi . Widardo ., Hudiyono ., Muthmainah ., Suyatmi A.A. Ketut Agung Cahyawan W Adi Magna Patriadi Nuhriawangsa AGYA GHILMAN FAZA Ahmad Ahid Mudayana Ahmad Ahid Mudayana Akhmad Azmiardi Alfi Yasmina Ali, Agni Shalha Amer Abdulwahab Al-aghbari, Nuha Amira, Nadya Kemala Anggraeni, Selvia Anis Nurhidayati Anshari, Shafira Yasmine Argyo Demartoto, Argyo Ari Natalia Probandari Ari Probandari Arsita Eka Prasetyawati Arsita Eka Prasetyawati Asaduddin, Aiman Hilmi Asih Anggraini Asih Anggraini Astrida Fesky Febrianty Astrika Yunita, Fresthy Bachnas, Muhammad Adrianes Berlian Permata Sakti Bhisma Murti Binti Maulina Binti Maulina Putri Brian Wasita Brian Wasita Brian Wasita Bustami, Mursyid Chintya Wulandarie Colti Sistiarani Colti Sistiarani Dara, Rizki Wulan Dedi Febriandaru Desy Puspa Putri Devi Ratna Mayasari DIAN LESTARI Didik Gunawan Tamtomo Dilma’aarij, Dilma’aarij Dyonisa Nasirochmi Pakha Endang Listyaningsih Suparyanti Erna Kristin Ernawati, Dwi Arini Eti Poncorini Pamungkasari Fairuz Zamani FAZA, AGYA GHILMAN Hamka, Dwi Wulandari Aaas Hanif Mustikasari Harioputro, Dhani Redhono Hariyanti, Lusiana Pradana Hartono Hartono Haruni, Diah Sekar Heni Hastuti Hudiyono . Hutami Sri Ummiyati Ida Nurwati Ignatius Gonggo Prihatmono Imanisa, Octavia Intan Indarta, Ardhia Fefrine Irma Prasetyowati iwan Dwiprahasto Kenneth Tan Khoiriyah, A'maliatul Kisrini . Komang Windayani Kristiani, Shinta Laksmi Wardhani, Tri Martya Ningrum Leli Saptawati Lilik Wijayanti Lilik Wijayanti Lilik Wijayanti Wijayanti Lisyawati, Shanti Lukman Aryoseto Ma&#039;rifatul Khoiriyah Ayda Maryati, Warsi Mashuri, Yusuf Ari Meintansari Manik MIFTAKHUL JANNAH Mochammad Fairuz Zahran Muchsin Doewes Mustara, Mustara Mustiqa Febriniata MUTHMAINAH . Muthmainah Muthmainah Nadya Kemala Amira Nafi'ah, Radifatun Naila Shofwati Putri Nanang Wiyono Nila Authoria Authoria Nita Dwi Astikasari Novelia Qothrunnada Novika, Revi Gama Hatta Nur Hafidha Hikmayani Nur Hafidha Hikmayani Nur Hasanah, Laras Eka Nur Hidayah Nurhasan Agung Prabowo Nurholis Majid Oktaviani oktaviani Paramasari Dirgahayu Paramasari Dirgahayu Poncorini Pamungkasari, Eti Primaningtyas, Widana Putri, Naila Shofwati Rahardjo, Setyo Sri Rio Nanda Pratama Rio Nanda Pratama Riris Arizka Wahyu Kumala Riris Arizka Wahyu Kumala, Riris Arizka Wahyu Rizaldy Pinzon RR. Ella Evrita Hestiandari Sakti, Berlian Permata Saptawati, Leli Satwika, Ariq Ratya Selvia Anggraeni Setyani, Rizka Ayu Setyani, Rizka Ayu Setyo Sri Rahardjo Setyo Sri Rahardjo Shinta Kristianti Shinta Retno Wulandari Shinta Shinta Sigit Setyawan Siswahyudianto Siti Fatimah R Solikah, Tri Agusti Sopianti, Densi Selpia Sri Darnoto Sri Mulyani Sri Mulyani Sugiarto Sumandjar, Tatar Sumardiyono Sumardiyono Sumardiyono Sumardiyono Suparyanti, Endang Listyaningsih Suparyanti, Endang Listyaningsih Susanto, Agnes Suyatmi . Syamsiar, Syamsiar Tri Agusti Sholikah Uki Retno Budihastuti Ummiyati, Hutami Sri Veronika Ika Budiastuti Vitri Widyaningsih Vitri Widyaningsih Wahyu Anjas Sari Wasita, Brian Widardo . Windayani, Komang Wiyono, Nanang Wulandari, Shinta Retno Yanuarini, Triatmi Andri Yulia Lanti Retno Dewi Yulia Sari