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Journal : Makara Journal of Science

Identification of Avian Influenza Genetic Resistance Gene Marker in Chickens Susanti, R; Utami, Nur Rahayu; Rahayuningsih, Margareta; Fibriana, Fidia
Makara Journal of Science Vol. 21, No. 4
Publisher : UI Scholars Hub

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Abstract

The Mx gene is a potential candidate as a genetic resistance marker to the avian influenza virus in chickens. The purpose of this study is to identify the potential of Mx gene as a marker of resistance to avian influenza viruses in various breeds of chicken. The study used an exploratory design using three common chicken breeds. The Kampong chicken, the Hy-Line Brown laying hen strain, and the White Leghorn broiler strain were collected from the local farms, and blood samples were drawn from each. The DNA of each sample was amplified by a pair of Mx gene primers (NE-F2/F and NE-R2/R). The polymerase chain reaction (PCR) product of Mx gene fragment was cut using restriction enzymes RsaI to determine the genotype of the Mx gene. The PCR-RFLP analysis in this study showed that 100% of the broilers were GG genotype. In laying hens, 80% were AG genotype, 10% GG genotype, and 10% of the sample was not successfully analyzed. In the Kampong chicken, 68.4% were GG genotype, and 31.6% were AG genotype. The results showed that DNA mutation identification can be applied to determine the genotype chicken against virus. The genotypes of Mx genes in chickens indicates avian influenza virus resistance; whereas, the chicken phenotype does not suggest their level of resistance.
Co-Authors aditya permana putra Agung Wibowo Aji, Septiko Aldias Bahatmaka Alma’ruf, Fattah Amin Retnoningsih Andin Vita Amalia, Andin Vita Anindya Ardiansari Annisa, Uma Apichat Upaichit Apichat Upaichit, Apichat Ari Yuniastuti Arif Widiyatmoko, Arif Bambang Subali BHAKTI KARYADI Binti Asalal, Nurzaimah Darmawan, Melissa Salma Deni Parlindungan Dewi Larasati, Dewi Dewi Mustikaningtyas Dewi, Novi Ratna Dyah Rini Indriyanti Eka Yulida Fitriani Erna Noor Savitri, Erna Noor Febriyanto, Hendra Hadi, Surtini Haris, Amnan Heriyanti, Andhina Putri Hidayat, Indra Nurul Hidayat, Luky Kholil, Putri Alifa Listiaji, Prasetyo Lutfia Nur Hadiyanti Lutfia Nur Hadiyanti, Lutfia Nur Manshurin, Abi Margareta Rahayuningsih Margareta Rahayuningsih Nugroho Edi K Martien Herna Susanti Mayangsari , Effelie Dewi Mayanti, Alfiana Nur Rosita Miranita Khusniati Muchamad Dafip Najda Aliyatun Naezak Nana Kariada Trimartuti Naufal, M Ahganiya Ningrum, Nuraini Septia Nirwana Nisa , Jauha Khoirun Novi Ratna Dewi Nur Rahayu Utami Nur Rahayu Utami Nurazizah, Kaylha Salsabilla Nurdian Susilowati Nursulistari, Siti Parmin - Prasdika, Tegar Oki Purwadi, Cintiya Egi Putri, Arifa Lutfiya Amanda R Susanti R Susanti R Susanti Ramadhan, Awaludin Pandu Retno Sri Iswari Rinda Fitri Handayani Rizki Nor Amelia Sandi Pratama , Fajar Rizqi Sari, Linda Siti Harnina Bintari Sofia, Aya Sudarmin Sudarmin Sunyoto Sunyoto Susanti - Susanti - Syah, Ahmad Firman Tirtasari, Ni Luh Tuti Widianti Tuti Widianti Umami, Hanifatul Wicaksono, Maulana Malik Wiyanto - Wulandari, Tiara Dwi Zaenal Abidin