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Antidiabetic Activity of Manonjaya Snakefruit Skin Extract with Zebra Fish (Danio rerio) as Animal Model Eti Rohaeti; Dea Nurafifah; Irmanida Batubara
Jurnal Jamu Indonesia Vol. 7 No. 3 (2022): Jurnal Jamu Indonesia
Publisher : Tropical Biopharmaca Research Center, IPB University

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.29244/jji.v7i3.201

Abstract

This research aimed to determine the active fraction and profile of active compounds in Manonjaya sSnakefruit skin extract that can be used as antidiabetic and the antidiabetic activity using zebra fish. Manonjaya sSnakefruit skin was extracted by maceration using 70% ethanol and then fractionated using n-hexane, ethyl acetate and methanol. The ethyl acetate fraction showed the highest yield and total phenolic content as well as the strongest α-glucosidase inhibition with an inhibition percentage of 64.43%. The ethyl acetate fraction provided the most active spot as an α-glucosidase inhibitor. The profile of active compounds that act as antidiabetic compound is flavonoid from glycosylflavone and flavone groups. Antidiabetic activity using zebra fish was indicates that Manonjaya Snakefruit skin extract from ethanol extract and ethyl acetate fraction can decrease blood sugar level of zebra fish after induced with alloxan. The crude extract group had 54.51% lower sugar content than the induction group and 41.70% lower than the negative control. Meanwhile, the ethyl acetate fraction group had 65.10% lower sugar content than the induction group and 55.27% lower than the negative control.
AKTIVITAS ANTIBAKTERI DAN TOKSISITAS MINYAK SERAI DAPUR (Cymbopogon citratus) DENGAN PERLAKUAN PEMEKATAN PADA SUHU BERBEDA Reni Febriani; Eti Rohaeti; Wulan Tri Wahyuni
Analit: Analytical and Environmental Chemistry Vol 6, No 2 (2021): Analit: Analytical and Environmental Chemistry
Publisher : Universitas Lampung Jl. Prof. Dr. Sumatri Brojonegoro No.1 Bandar Lampung

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (619.813 KB) | DOI: 10.23960/aec.v6i2.2021.p168-179

Abstract

Minyak atsiri serai (Cymbopogon citratus) dapur telah dipergunakan secara luas di berbagai industri, diantaranya sebagai agen antibakteri. Pada penelitian ini dievaluasi aktivitas antibakteri dan toksisitas minyak serai dapur yang dipekatkan pada suhu berbeda. Toksisitas dievaluasi melalui uji brine shrimp lethality test (BSLT), sementara uji antibakteri dilakukan terhadap bakteri Staphylococcus aureus dan S. epidermidis dengan metode difusi dan dilusi. Komponen kimia yang terdapat dalam minyak serai dianalisis menggunakan kromatografi gas spektrometri massa (GC-MS). Hasil yang diperoleh menunjukkan berdasarkan uji BSLT diperoleh nilai lethal concentration 50 (LC50) sampel minyak serai dapur semua perlakuan < 1000 ppm dengan LC50 terendah sebesar 16,53 ± 2,82 ppm. Nilai konsentrasi hambat minimun (KHM) dan konsentrasi bunuh minimum (KBM) minyak serai dapur terhadap S. aureus umumnya lebih rendah dibanding terhadap S. epidermidis. Aktivitas antibakteri minyak serai dapur terhadap S. aureus terkategori sangat kuat dengan nilai KHM dan KBM terendah ditunjukkan oleh minyak serai tanpa perlakuan pemekatan yaitu sebesar 1250 dan 2500 ppm terhadap S. aureus. Komposisi senyawa kimia utama dalam minyak serai dapur berdasarkan analisis GC-MS ialah sitral, neral dan β-myrcenehttp://dx.doi.org/10.23960/aec.v6.i2.2021.p168-179
MODIFIKASI SCREEN PRINTED CARBON ELECTRODE DENGAN POLIMER TERCETAK MOLEKUL (MIP) DAN APLIKASINYA UNTUK DETEKSI GLUTATION Eti Rohaeti; Wulan Tri Wahyuni; Moh Arif Rohman Nur Hakim
Analit: Analytical and Environmental Chemistry Vol 3, No 1 (2018): Analit: Analytical and Environmental Chemistry
Publisher : Universitas Lampung Jl. Prof. Dr. Sumatri Brojonegoro No.1 Bandar Lampung

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (461.832 KB) | DOI: 10.23960/aec.v3i1.2018.p

Abstract

Penelitian ini bertujuan memodifikasi screen printed carbon electrode (SPCE)  dengan polimer tercetak molekul (MIP) dan mengaplikasikannya untuk deteksi glutation tereduksi (GSH) secara tidak langsung dengan mediator bromin menggunakan teknik voltammetri siklik. SPCE dimodifikasi dengan MIP polipirola (MIPpy) melalui teknik elektrodeposisi. Pengukuran GSH dilakukan dalam elektrolit NaClO4 0.2 M yang mengandung 1 mM NaBr. Hasil yang diperoleh menunjukkan SPCE-MIPpy telah berhasil diperoleh melalui teknik elektrodeposisi. Pada pengukuran diperoleh puncak oksidasi bromida pada potensial ±0.63 V vs. Ag/AgCl. Puncak ini berkorelasi linear dengan konsentrasi GSH dalam larutan sehingga dapat digunakan sebagai dasar penentuan GSH. Polipirola meningkatkan konduktivitas listrik elektrode sehingga dapat meningkatkan puncak arus oksidasi GSH. Pengukuran glutation dengan SPCE-MIPpy menunjukkan limit deteksi dan limit kuantitasi yang lebih baik dibandingkan SPCE, yaitu sebesar 7.6626 µM dan 25.4092 µM. Keberadaan interference glukosa tidak mengganggu pengukuran glutation. Kinerja SPCE-MIPpy dalam pengukuran GSH disimpulkan lebih optimum dibandingkan SPCE.  DOI: http://dx.doi.org/10.23960/aec.v3.i1.2018.p47-60 
The Effect of Different Hydroponic Types and Nutrient Concentrations on the Chemical Composition and Antioxidant Capacity of Purwoceng (Pimpinella pruatjan) Extract Irmanida Batubara; Shadila Fira Asoka; Eni Sumarni; Herry Suhardiyanto; Mohamad Solahudin; Slamet Widodo; Supriyanto; Eti Rohaeti; Yudiwanti; Folkes Laumal; Erniati
HAYATI Journal of Biosciences Vol. 30 No. 5 (2023): September 2023
Publisher : Bogor Agricultural University, Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.4308/hjb.30.5.985-994

Abstract

Purwoceng (Pimpinella pruatjan) is an Indonesian native plant but difficult to cultivate. Hydroponics can be used as a solution for purwoceng cultivation. Hydroponic types and nutrient concentrations can affect purwoceng’s productivity. This study aimed to determine total phenolic content (TPC) and total flavonoid content (TFC), changes in steroid, and antioxidant capacity due to two hydroponic types (nonrecirculating and recirculating drip) and three nutrient concentrations (1,000, 1,500, and 2,000 ppm). Purwoceng was determined for its moisture content, ash content, and dry weight, and extracted with ethyl acetate for gas chromatography-mass spectrometry analysis and with ethanol for TPC, TFC, and antioxidant capacity. The data were analyzed statistically and grouped using principal component analysis (PCA) and heat map analysis (HMA). Nonrecirculating drip with high nutrient concentration at the aerial part was the best treatment to produce high TPC, TFC, and antioxidant capacity. There were no significant changes in steroid content. PCA showed that purwoceng extracts could be grouped in to two groups, i.e. aerial and root groups. HMA showed that purwoceng extracts could be distinguished from the abundance of palmitic acid and stigmasterol. The antioxidant capacity of purwoceng was directly proportional to the TPC value and also related to the presence of phytol, stigmasterol, and palmitic acid.
Antioxidant Activity of Jojoba (Simmondsia chinensis) Seed Residue Extract Arnold Patogi Siahaan; Eti Rohaeti; Ali Mahmoud Muddathir; Irmanida Batubara
Biosaintifika: Journal of Biology & Biology Education Vol 12, No 3 (2020): December 2020
Publisher : Department of Biology, Faculty of Mathematics and Sciences, Semarang State University . Ro

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.15294/biosaintifika.v12i3.23323

Abstract

Jojoba (Simmondsia chinensis (Link)) is a shrub plant that widely used as cosmetic ingredients especially jojoba oil. When making jojoba oil, the residue still reminds and become waste. This study aimed to determine the antioxidant activity of jojoba seed residue (JSR). Jojoba seed residue was collected from Sudan. The JSR extracted by maceration with n-hexane, ethyl acetate, and 70% ethanol solvent. The antioxidant activity was determined using DPPH (2,2-diphenyl-1-picrylhydrazyl) assay. The result showed that 70% ethanol extract of JSR had the highest antioxidant activity with 50% Inhibition Concentration (IC50) value of 219.42 mg/L. The 70% ethanol extract was fractionated by using silica gel column chromatography with gradient elution produced 10 fractions. All fractions showed antioxidant activity (IC50106-447 mg/L). Fraction 3 had the highest antioxidant activity with IC50 value of 106.26 mg/L. Therefore, JSR has a great potential for use as a raw material in cosmetic and pharmaceutical products industry, or as a source of bioactive compounds.
Detection of Hexavalent Chromium Ion in Water by Optode Membrane Arif, Zulhan; Munandar, Rafly; Rohaeti, Eti; Rafi, Mohamad
Indonesian Journal of Chemical Studies Vol. 2 No. 2 (2023): Indones. J. Chem. Stud., December 2023
Publisher : Indonesian Scholar Society

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.55749/ijcs.v2i2.39

Abstract

Chromium is a heavy metal that is often found as a water pollutant. High concentrations of hexavalent chromium (Cr(VI)) ion in nature are toxic and carcinogenic, so their presence in water needs to be monitored. Detection of heavy metals in water was carried out using an optical sensor membrane (optode). The optode was fabricated from cellulose triacetate polymer with plasticizers (oleic acid and acetophenone), aliquot 336, and the chromoionophore 1.5-diphenylcarbazide (DPC). The success synthesis of optode was evidenced by FTIR and SEM characterization. The optode performance produces a linear response in detecting Cr(VI) ion in the concentration range of 0.02-0.40 mg/L with an R2 of 0.9930, as well as the best conditioning at pH 3. The detection and quantitation limits are 0.0055 mg/L and 0.0165 mg/L. The sensitivity of the chromium optode was excellence, with a molar absorptivity value of 8.8303 × 106 M-1cm-1. The performance test results of the chromium optode were acceptable because they meet the specified requirements for minimum detection concentration value.
Simple Synthesis of Cellulose Triacetate from HVS Paper Waste and Its Application for Optode Arif, Zulhan; Fendy; Akhmad Aghzath, Ananjaya; Saprudin, Deden; Rohaeti, Eti
Indonesian Journal of Chemical Studies Vol. 3 No. 1 (2024): Indones. J. Chem. Stud., June 2024
Publisher : Indonesian Scholar Society

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.55749/ijcs.v3i1.46

Abstract

The optode membrane is a membrane that can identify ions in an aqueous solution. One of the most widely used optode membranes is cellulose triacetate based. Cellulose triacetate (CTA) has the characteristics of hydrophobic, transparent, elastic, and affordable. There are sources of cellulose triacetate that can be utilized and waste paper is one of them. Waste paper is extracted to obtain the cellulose, then by acetylization reaction to produce cellulose triacetate. The resultant cellulose triacetate has a degree of substitution (DS) of 2.89 and an acetyl group percentage (% AG) of 43.64. The standard and synthesized CTA optode membrane are tested for performance with various parameters, ie optimum pH, optimum immersion time, working range, limit of detection (LOD), and limit of quantification (LOQ). The standard CTA optode membranes and synthesized worked at pH 3 and 4 with optimum immersion time for 15 min, respectively. The optode produced a linear response in detecting Cr(VI) ion in the concentration range of 0.02-1 mg/L for standard CTA with an R2 of 0.9726 and 1-25 mg/L for synthesized CTA with an R2 of 0.9764, The limit of detection (LOD) and limit of quantitation (LOQ) were 0.0015 mg/L and 0.0051 mg/L for standard CTA, while 0.0224 mg/L and 0.0749 mg/L for synthesis CTA respectively. Since both optode membranes' performance test results are adequate, the synthesis results of CTA optode membranes can be employed as one source of cellulose triacetate.
Faktor-Faktor Yang Berpengaruh Terhadap Turnover Intention Perawat Rohaeti, Eti; Novita, Astrid
Jurnal Ilmiah Kebidanan Indonesia Vol 11 No 01 (2021): Jurnal Ilmiah Kebidanan Indonesia (Indonesian Midwifery Scientific Journal) Sek
Publisher : Q PRESS

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.33221/jiki.v11i01.928

Abstract

Salah satu masalah yang berpengaruh dengan tenaga kerja dalam suatu organisasi adalah tingkat turnover yang tinggi. Data turnover perawat Rumah Sakit Permata Depok yang diperoleh adalah dari tahun 2016 sampai dengan tahun 2018 yaitu tahun 2016 didapatkan persentasi turnover sebesar 29%, tahun 2017 sebesar 20 % dan tahun 2018 sebesar 28%. Tujuan penelitian ini untuk mengetahui pengaruh langsung dan tidak langsung serta besar annya antara kepemimpinan, lingkungan kerja, beban kerja dan kepuasan kerja perawat terhadap turnover intention perawat di Rumah Sakit Permata Depok Tahun 2019. Metode yang digunakan dalam penelitian ini adalah pendekatan kuantitatif yang menggunakan desain cross-setional. Sampel yang digunakansebanyak 90 Perawat sebagai responden. Metode analisis yang digunakan adalah Structural Equation Model (SEM) diolah dengan mengunakan software SmartPLS 2.0 dan SPSS 20. Hasil pengujian hipotesis dengan SEM dan pengolahan data menggunakan software SmartPLS , didapat temuan bahwa variabel Turnover Intention perawat di Rumah Sakit Permata Depok dipengaruhi oleh Kepemimpinan (16,54%), lingkungan kerja (-6,72%), beban kerja (33,83%) dan kepuasan kerja (32,46%).Beban kerja merupakan faktor yang paling berpengaruh terhadap turnover intention perawat. Semakin tinggi tingkat beban kerja perawat, maka semakin tinggi turnover intention perawat dan sebaliknya semakin rendah tingkat beban kerja perawat, maka semakin rendah turnover intention perawat di Rumah Sakit Permata Depok. Untuk mengurangi beban kerja perawat yang tinggi, sebaiknya pihak rumah sakit mengembalikan peran dan fungsi Perawat sesuai dengan kompetensinya yaitu memberikan asuhan keperawatan kepada pasien dan keluarga, mengurangi pekerjaan administratif yang bukan tanggung jawab perawat. Salah satu masalah yang berpengaruh dengan tenaga kerja dalam suatu organisasi adalah tingkat turnover yang tinggi. Data turnover perawat Rumah Sakit Permata Depok yang diperoleh adalah dari tahun 2016 sampai dengan tahun 2018 yaitu tahun 2016 didapatkan persentasi turnover sebesar 29%, tahun 2017 sebesar 20 % dan tahun 2018 sebesar 28%. Tujuan penelitian ini untuk mengetahui pengaruh langsung dan tidak langsung serta besar annya antara kepemimpinan, lingkungan kerja, beban kerja dan kepuasan kerja perawat terhadap turnover intention perawat di Rumah Sakit Permata Depok Tahun 2019. Metode yang digunakan dalam penelitian ini adalah pendekatan kuantitatif yang menggunakan desain cross-setional. Sampel yang digunakansebanyak 90 Perawat sebagai responden. Metode analisis yang digunakan adalah Structural Equation Model (SEM) diolah dengan mengunakan software SmartPLS 2.0 dan SPSS 20. Hasil pengujian hipotesis dengan SEM dan pengolahan data menggunakan software SmartPLS , didapat temuan bahwa variabel Turnover Intention perawat di Rumah Sakit Permata Depok dipengaruhi oleh Kepemimpinan (16,54%), lingkungan kerja (-6,72%), beban kerja (33,83%) dan kepuasan kerja (32,46%).Beban kerja merupakan faktor yang paling berpengaruh terhadap turnover intention perawat. Semakin tinggi tingkat beban kerja perawat, maka semakin tinggi turnover intention perawat dan sebaliknya semakin rendah tingkat beban kerja perawat, maka semakin rendah turnover intention perawat di Rumah Sakit Permata Depok. Untuk mengurangi beban kerja perawat yang tinggi, sebaiknya pihak rumah sakit mengembalikan peran dan fungsi Perawat sesuai dengan kompetensinya yaitu memberikan asuhan keperawatan kepada pasien dan keluarga, mengurangi pekerjaan administratif yang bukan tanggung jawab perawat.
A Brief Review on Fabrication of Screen-Printed Carbon Electrode: Materials and Techniques Wahyuni, Wulan Tri; Putra, Budi Riza; Fauzi, Achmad; Ramadhanti, Desi; Rohaeti, Eti; Heryanto, Rudi
Indonesian Journal of Chemical Research Vol 8 No 3 (2021): Edition for January 2021
Publisher : Jurusan Kimia, Fakultas Sains dan Teknologi, Universitas Pattimura

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.30598/ijcr.2021.8-wul

Abstract

Screen-printed carbon electrode (SPCE) is one of the most interesting designs to combine a working (from carbon based material), reference, and counter electrode in a single-printed substrate. SPCE has been used in many electrochemical measurements due to its advantages for analysis in microscale. This paper summarises the main information about SPCE fabrication from the material and fabrication technique aspect on the flat substrate based on the work that has been published in the last 30 years. The success of SPCE fabrication is highly dependent on the composition of conductive ink which consists of conductive materials, binder, and solvents; substrate; and fabrication techniques. Among the carbon-based materials, the most widely used for SPCE fabrications are graphite, graphene, and carbon nanotubes. The frequent binder used are polymer-based materials such as polystyrene, polyaniline, poly 3,4-ethylenedioxythiophene:polystyrene sulfonate (PEDOT:PSS), and polyvinyl chloride. The solvents used for SPCE fabrication are varied including water and various organic solvents. The main characteristics of the SPCE substrate should be inert in order to avoid any interferences during electrochemical measurements. The screen printing and inkjet printing technique are preferred for SPCE fabrication due to easy fabrication and the possibility for mass production of SPCE.
Development of Differential Pulse Anodic Stripping Voltammetry Technique for Cadmium(II) Detection and Its Application in Water Spinach Putra, Budi Riza; Aristri, Manggar Arum; Rohaeti, Eti; Wahyuni, Wulan Tri
Indonesian Journal of Chemical Research Vol 9 No 2 (2021): Edition for September 2021
Publisher : Jurusan Kimia, Fakultas Sains dan Teknologi, Universitas Pattimura

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.30598/ijcr.2021.9-riz

Abstract

Cadmium is a toxic pollutant that is harmful to the environment and humans. The purpose of this research was to develop a method for cadmium(II) detection using differential pulse anodic stripping voltammetry (DPASV) using a glassy carbon electrode. The developed method was then applied for cadmium detection in the vegetable samples which is water spinach. The developed method was optimized in several parameters such as potential window, deposition potential, deposition time, and scan rate. The developed method for cadmium(II) detection was also investigated in its analytical performance includes linearity, precision, detection limit, and quantitation limit. The optimum conditions for cadmium(II) detection in 0.1 M KCl using DPASV technique obtained such as potential window from -1200 to -100 mV, deposition potential of -1100 mV (vs Ag/AgCl), and deposition time of 360 s. It was obtained good linearity for cadmium(II) detection using the DPASV technique with an R2 of 0.996. The precision was expressed as %SBR with 0.66%. The detection and quantitation limits for cadmium(II) detection were 0.4206 µM~0.0771 ppm and 0.5525 µM~0.1013 ppm, respectively. The developed method was then applied for cadmium(II) measurement in the water spinach sample and the obtained cadmium(II) concentration in water spinach was 0.2399 mg/Kg.
Co-Authors A. KUSNANTO Abdul Rohman Achmad Fauzi Achmad Fauzi Ade Heri Mulyati, Ade Heri Aditya Kurniawan Aditya Kurniawan Agustina, Rofiqoh Inayati Aini, Nazwa Aisyah Akhmad Aghzath, Ananjaya Ali Mahmoud Muddathir Ali Mahmoud Muddathir Ali Miftahudin Anak Agung Gede Sugianthara Antonio Kautsar Arinana Arinana Arnold Patogi Siahaan Arnold Patogi Siahaan Aryo Tedjo Asih Gayatri Astrid Novita, Astrid Bambang Prajogo Eko Wardoyo Budi Riza Putra Budi Riza Putra Budi Riza Putra Budi Santoso, Arianto Casnan Casnan Casnan, Casnan Citra Ajeng Palupi Dea Nurafifah Dea Nurafifah Deden Saprudin Derry Permana Dewi Anggraeni Septaningsih Diposani, Diva Putri Djeni Hendra Djeni Hendra Djoko Santoso Dodi Nandika Dudy Imanuddin Effendi Dyah Iswantini Eni Sumarni Erliza Noor Erniati Fadila Karunina Febriani, Elsa Fendy Folkes Laumal Gunawan, Fransisca Hadjomidjojo, Hartrisari Hafshah, Nurul Hartrisari Hadjomidjojo Hartrisari Hardjomidjojo Herry Suhardiyanto Ibrahim Ibrahim Ibrahim Ida Ayu Putu Sri Widnyani Irene, Angelina Irma Isnafia Arief Irmanida Batubara Irzaman, Irzaman Jaharuddin Jaharuddin, Jaharuddin Karomah, Alfi Hudatul Khoirotul Muzayanah KUSNANTO, A. Latifah K Darusman Latifah K Darusman Latifah K. Darusman Latifah K. Darusman Latifah Kosim Darusman Latifah Kosim Darusman Lina Karlinasari Linda Trivana Linda Trivana Linda Trivana, Linda Lucia Dhiantika Witasari, Lucia Dhiantika M. Rafi Madya, Muhammad Miftahul Makmum Syafi’i Manggar Arum Aristri Mardiana, Eni Moh Arif Rohman Nur Hakim Moh Nur Hakim Mohamad Rafi Mohamad Solahudin Muddathir, Ali Mahmoud Muhammad Bachri Amran Munandar, Rafly N. G. Pamungkas Novia Rahma Nur Adi Saputra Nur Adi Saputra Nurdiani Nurdiani, Nurdiani Nurhajawarsi Nurhajawarsi Nursifa Mawadah Purnawan Purnawan Purnawan Purnawan Putri, Silvi Agusri Rachmayanti, Yanti Rahadyanoto Trimulia Rahmawati, Heni Rahmawati, Wahyu Riski Ramadhanti, Desi Reni Febriani Reni Febriani, Reni Rinaldi Idroes Rudi Heryanto Santoso, Arianto Budi Satriah, Lilis - Septaningsih, Dewi Anggraini Shadila Fira Asoka Siahaan, Arnold Patogi Silvia, Riva Siti Aisyah Slamet Widodo Sri Sugiarti Supriyanto Syafi’i, Makmum Syifa Muflihah Tamida Elinda Taopik Ridwan Trisni Untari Dewi Ummu Salma Al Azizah Uswatun Hasanah Utami Dyah Syafitri Wulan Tri Wahyuni Yudiwanti Yuspian Nur Zaenal Abidin